Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= P5PG1031
         (668 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase f...    48   6e-06
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ...    33   0.17 
At1g72590.1 68414.m08394 3-oxo-5-alpha-steroid 4-dehydrogenase f...    30   1.6  
At4g14250.1 68417.m02198 UBX domain-containing protein low simil...    29   2.1  
At3g04310.1 68416.m00456 expressed protein                             29   2.8  
At4g20240.1 68417.m02957 cytochrome P450, putative similar to |C...    28   6.5  

>At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein / steroid 5-alpha-reductase family
           protein similar to synaptic glycoprotein SC2 spliced
           variant from Homo sapiens [EMBL:AF038958], SC2 from
           Rattus sp. [gi:256994]; contains Pfam
           3-oxo-5-alpha-steroid 4-dehydrogenase domain PF02544
          Length = 310

 Score = 48.0 bits (109), Expect = 6e-06
 Identities = 25/64 (39%), Positives = 31/64 (48%), Gaps = 6/64 (9%)
 Frame = -2

Query: 667 NFVSCPNYTYEFGSWLFFTIMTKCAPAGLFAAAGFYQMAVWAIGKHRNYKKEF------P 506
           N V+C NYT E   WL F I T+     +F A     M  WA+GKH   +K F      P
Sbjct: 238 NIVTCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKP 297

Query: 505 DYPK 494
            YP+
Sbjct: 298 KYPR 301


>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
           putative / steroid 5-alpha-reductase, putative identical
           to gi:1280611; contains a steroid 5-alpha reductase,
           C-terminal domain
          Length = 262

 Score = 33.1 bits (72), Expect = 0.17
 Identities = 24/62 (38%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
 Frame = -2

Query: 661 VSCPNYTYEFGSWLFFTIMT-KCAPAGLFAAAGFYQMAVWAIGKHRNYKKEF-PDYPKGR 488
           VSCPNY  E   WL + +MT   A  G F       +   A   H+ Y  +F  +YPK R
Sbjct: 196 VSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYT-CSNLFPRARASHKWYIAKFKEEYPKTR 254

Query: 487 KA 482
           KA
Sbjct: 255 KA 256


>At1g72590.1 68414.m08394 3-oxo-5-alpha-steroid 4-dehydrogenase
           family protein / steroid 5-alpha-reductase family
           protein similar to Arabidopsis thaliana DET2
           [gi:1280611]; contains Pfam 3-oxo-5-alpha-steroid
           4-dehydrogenase domain PF02544
          Length = 320

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
 Frame = -2

Query: 661 VSCPNYTYEFGSWLFFTIMTKCAPAGLFAAAGFYQMAV-WAIGK-HRNYKKEFPDYPKGR 488
           VSCP++  E   +L   I +      ++   GF    + +A G+ HR Y ++F +YP  R
Sbjct: 253 VSCPHFLAEIVLYLGLLISSGGTDISIWLLFGFVAANLTYAAGETHRWYLQKFENYPASR 312

Query: 487 KA 482
            A
Sbjct: 313 HA 314


>At4g14250.1 68417.m02198 UBX domain-containing protein low
           similarity to 60S ribosomal protein L2 [Nicotiana
           tabacum] GI:9230281; contains Pfam profile PF00789: UBX
           domain
          Length = 724

 Score = 29.5 bits (63), Expect = 2.1
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = -3

Query: 555 WPFGPSVNIATTRRNSQTTPKAVKLFSHSFYK 460
           W  GP  NIA+  RN +T      L S++FY+
Sbjct: 523 WDAGPHENIASLTRNRRTETAETCLSSYNFYE 554


>At3g04310.1 68416.m00456 expressed protein
          Length = 269

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 10/30 (33%), Positives = 15/30 (50%)
 Frame = -3

Query: 387 WSNKKFTNIDIDNFDISNRYWLVPWWVSDD 298
           W  + F +    +F+  NR    PWW+ DD
Sbjct: 47  WEEELFRDEGFASFEFGNRKKKRPWWLDDD 76


>At4g20240.1 68417.m02957 cytochrome P450, putative similar to
           |C71R_ARATH Cytochrome P450 71A27 (SP:O65438)
           [Arabidopsis thaliana]
          Length = 865

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = +3

Query: 441 IKLSNLIYRMNGRIALRPLG 500
           + L+N +YR N R+  RPLG
Sbjct: 842 VGLANFVYRFNWRVETRPLG 861


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,618,006
Number of Sequences: 28952
Number of extensions: 249542
Number of successful extensions: 510
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 501
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 510
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)