BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG1017 (631 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1281.06c |||acyl-coA desaturase |Schizosaccharomyces pombe|c... 103 2e-23 SPAC1687.16c |erg3||C-5 sterol desaturase Erg3 |Schizosaccharomy... 32 0.059 SPBC27B12.03c |||lathosterol oxidase |Schizosaccharomyces pombe|... 29 0.73 SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 28 1.3 SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 27 3.0 SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 27 3.0 SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo... 26 3.9 SPAC19D5.05c |||U3 snoRNP-associated protein Imp3 |Schizosacchar... 26 5.2 SPAC1687.02 |||CAAX prenyl protease |Schizosaccharomyces pombe|c... 26 5.2 SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizo... 25 6.8 SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces po... 25 9.0 >SPCC1281.06c |||acyl-coA desaturase |Schizosaccharomyces pombe|chr 3|||Manual Length = 479 Score = 103 bits (248), Expect = 2e-23 Identities = 45/120 (37%), Positives = 74/120 (61%) Frame = +2 Query: 269 FLNTIFTWVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDI 448 F +I W RDHR HH+YTDTD DP+N +GF+++H+GW+++ ++P + G+S D+SD+ Sbjct: 136 FEGSIRWWSRDHRAHHRYTDTDKDPYNVKKGFWYAHVGWMIILQNP--RRIGRS-DVSDL 192 Query: 449 YCNPLLRFQKKYAIPFIGTICFIIPTLAPMYFWGESLNNAWHITVLRYIFSLNGTFLVNS 628 +P + F ++ +P + FI P+L WG+ ++ V R +F + TF VNS Sbjct: 193 NSDPFVMFNHRHFLPIASFMAFIFPSLFCGLLWGDYRGGYFYAGVCRLVFVHHATFCVNS 252 >SPAC1687.16c |erg3||C-5 sterol desaturase Erg3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 300 Score = 32.3 bits (70), Expect = 0.059 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = +2 Query: 257 WLHRFLNTIFTWVRDHRLHHKY 322 W HRFL+ + + R H+LHHK+ Sbjct: 135 WAHRFLHHRYVYPRLHKLHHKW 156 >SPBC27B12.03c |||lathosterol oxidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 329 Score = 28.7 bits (61), Expect = 0.73 Identities = 13/41 (31%), Positives = 24/41 (58%) Frame = +2 Query: 200 AIYILLVYMDFISVSPQQNWLHRFLNTIFTWVRDHRLHHKY 322 +I + L++ DF+ W+HR L+ + + H+LHHK+ Sbjct: 154 SIALFLLFSDFLIY-----WIHRALHHRWLYAPLHKLHHKW 189 >SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 471 Score = 27.9 bits (59), Expect = 1.3 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +1 Query: 76 SVDKTNETEYLKDNHVDYEKLIAPQASPIKHKIVVM 183 S+ N EYL D+ V+ KL + P+K K++ + Sbjct: 28 SLTSINSAEYLYDSFVEISKLPLEKKLPLKWKLITV 63 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 26.6 bits (56), Expect = 3.0 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = -3 Query: 389 TTSRCVRKKNLS*HYEGLHQC 327 T + V KKN+S H+EG+ +C Sbjct: 1538 TDALLVLKKNISMHFEGVEEC 1558 >SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1463 Score = 26.6 bits (56), Expect = 3.0 Identities = 26/97 (26%), Positives = 37/97 (38%), Gaps = 4/97 (4%) Frame = +2 Query: 326 DTDADPHNATRGFFFSHI--GWLLVRKHPMVKIAGKS-LDMSDIYCNPLLRFQKKYAIPF 496 +T P T FS I GWL P++K ++ L SD + P I F Sbjct: 169 ETGLRPSRFTYANIFSRISFGWL----SPLMKFGYRNYLTESDAWSLPPAERSSNLTIVF 224 Query: 497 IGT-ICFIIPTLAPMYFWGESLNNAWHITVLRYIFSL 604 I + +Y WG N W +TV+ + L Sbjct: 225 EKNWISHAKKKKSSLYMWGVLFLNHWKLTVVIIVLKL 261 >SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharomyces pombe|chr 3|||Manual Length = 1369 Score = 26.2 bits (55), Expect = 3.9 Identities = 11/28 (39%), Positives = 15/28 (53%) Frame = +2 Query: 539 YFWGESLNNAWHITVLRYIFSLNGTFLV 622 +FWG L W I V+ F L G +L+ Sbjct: 1181 FFWGSKLTKLWKIVVMTVSF-LVGAWLI 1207 >SPAC19D5.05c |||U3 snoRNP-associated protein Imp3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 183 Score = 25.8 bits (54), Expect = 5.2 Identities = 15/47 (31%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Frame = -3 Query: 494 MVLRTFSEILKEDYNKYLTCQGSFQQFS-PLDVFAPTTSRCVRKKNL 357 MV+R + +E+Y KY G F+Q + L + PT ++ +NL Sbjct: 31 MVMRRYHISKREEYQKYNIICGKFRQLAHRLSLLDPTDPFRLQYENL 77 >SPAC1687.02 |||CAAX prenyl protease |Schizosaccharomyces pombe|chr 1|||Manual Length = 271 Score = 25.8 bits (54), Expect = 5.2 Identities = 17/56 (30%), Positives = 26/56 (46%) Frame = +1 Query: 118 HVDYEKLIAPQASPIKHKIVVMNVIRFSYLHIAGLYGLYLCFTSAKLATSVFEHHF 285 H YE L+A + I + V++FSY + G Y +L ++ L S H F Sbjct: 162 HHTYEFLLAYPNAYIA--AALQTVVQFSYTTVFGWYTTHLFLSTHSLFPSFLVHAF 215 >SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 456 Score = 25.4 bits (53), Expect = 6.8 Identities = 13/23 (56%), Positives = 15/23 (65%) Frame = -3 Query: 428 SFQQFSPLDVFAPTTSRCVRKKN 360 SF +PL VFAP TSR +R N Sbjct: 201 SFDPLAPL-VFAPNTSRVLRVTN 222 >SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces pombe|chr 1|||Manual Length = 639 Score = 25.0 bits (52), Expect = 9.0 Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 3/41 (7%) Frame = +1 Query: 70 PNSVDKTNETEYLKDNHVDYE---KLIAPQASPIKHKIVVM 183 PN V N + + NH+D E KLI + ++ +I V+ Sbjct: 589 PNQVSMPNISNIVSTNHLDVETQMKLIMDENQRLREQIAVL 629 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,703,891 Number of Sequences: 5004 Number of extensions: 58311 Number of successful extensions: 145 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 142 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 144 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 279695522 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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