BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG1014 (607 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1281.06c |||acyl-coA desaturase |Schizosaccharomyces pombe|c... 92 7e-20 SPAC1687.16c |erg3||C-5 sterol desaturase Erg3 |Schizosaccharomy... 32 0.057 SPBC27B12.03c |||lathosterol oxidase |Schizosaccharomyces pombe|... 29 0.70 SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 28 1.2 SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 27 2.8 SPAC19D5.05c |||U3 snoRNP-associated protein Imp3 |Schizosacchar... 26 4.9 SPAC1687.02 |||CAAX prenyl protease |Schizosaccharomyces pombe|c... 26 4.9 SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizo... 25 6.5 SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces po... 25 8.6 >SPCC1281.06c |||acyl-coA desaturase |Schizosaccharomyces pombe|chr 3|||Manual Length = 479 Score = 91.9 bits (218), Expect = 7e-20 Identities = 37/95 (38%), Positives = 62/95 (65%) Frame = +2 Query: 314 FLNTIFTWVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDI 493 F +I W RDHR HH+YTDTD DP+N +GF+++H+GW+++ ++P + G+S D+SD+ Sbjct: 136 FEGSIRWWSRDHRAHHRYTDTDKDPYNVKKGFWYAHVGWMIILQNP--RRIGRS-DVSDL 192 Query: 494 YCNPLLRFQKKYAIPFIGTICFIIPTLAPMYFWGE 598 +P + F ++ +P + FI P+L WG+ Sbjct: 193 NSDPFVMFNHRHFLPIASFMAFIFPSLFCGLLWGD 227 >SPAC1687.16c |erg3||C-5 sterol desaturase Erg3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 300 Score = 32.3 bits (70), Expect = 0.057 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = +2 Query: 302 WLHRFLNTIFTWVRDHRLHHKY 367 W HRFL+ + + R H+LHHK+ Sbjct: 135 WAHRFLHHRYVYPRLHKLHHKW 156 >SPBC27B12.03c |||lathosterol oxidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 329 Score = 28.7 bits (61), Expect = 0.70 Identities = 13/41 (31%), Positives = 24/41 (58%) Frame = +2 Query: 245 AIYILLVYMDFISVSPQQNWLHRFLNTIFTWVRDHRLHHKY 367 +I + L++ DF+ W+HR L+ + + H+LHHK+ Sbjct: 154 SIALFLLFSDFLIY-----WIHRALHHRWLYAPLHKLHHKW 189 >SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 471 Score = 27.9 bits (59), Expect = 1.2 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +1 Query: 121 SVDKTNETEYLKDNHVDYEKLIAPQASPIKHKIVVM 228 S+ N EYL D+ V+ KL + P+K K++ + Sbjct: 28 SLTSINSAEYLYDSFVEISKLPLEKKLPLKWKLITV 63 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 26.6 bits (56), Expect = 2.8 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = -3 Query: 434 TTSRCVRKKNLS*HYEGLHQC 372 T + V KKN+S H+EG+ +C Sbjct: 1538 TDALLVLKKNISMHFEGVEEC 1558 >SPAC19D5.05c |||U3 snoRNP-associated protein Imp3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 183 Score = 25.8 bits (54), Expect = 4.9 Identities = 15/47 (31%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Frame = -3 Query: 539 MVLRTFSEILKEDYNKYLTCQGSFQQFS-PLDVFAPTTSRCVRKKNL 402 MV+R + +E+Y KY G F+Q + L + PT ++ +NL Sbjct: 31 MVMRRYHISKREEYQKYNIICGKFRQLAHRLSLLDPTDPFRLQYENL 77 >SPAC1687.02 |||CAAX prenyl protease |Schizosaccharomyces pombe|chr 1|||Manual Length = 271 Score = 25.8 bits (54), Expect = 4.9 Identities = 17/56 (30%), Positives = 26/56 (46%) Frame = +1 Query: 163 HVDYEKLIAPQASPIKHKIVVMNVIRFSYLHIAGLYGLYLCFTSAKLATSVFEHHF 330 H YE L+A + I + V++FSY + G Y +L ++ L S H F Sbjct: 162 HHTYEFLLAYPNAYIA--AALQTVVQFSYTTVFGWYTTHLFLSTHSLFPSFLVHAF 215 >SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 456 Score = 25.4 bits (53), Expect = 6.5 Identities = 13/23 (56%), Positives = 15/23 (65%) Frame = -3 Query: 473 SFQQFSPLDVFAPTTSRCVRKKN 405 SF +PL VFAP TSR +R N Sbjct: 201 SFDPLAPL-VFAPNTSRVLRVTN 222 >SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces pombe|chr 1|||Manual Length = 639 Score = 25.0 bits (52), Expect = 8.6 Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 3/41 (7%) Frame = +1 Query: 115 PNSVDKTNETEYLKDNHVDYE---KLIAPQASPIKHKIVVM 228 PN V N + + NH+D E KLI + ++ +I V+ Sbjct: 589 PNQVSMPNISNIVSTNHLDVETQMKLIMDENQRLREQIAVL 629 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,528,751 Number of Sequences: 5004 Number of extensions: 52413 Number of successful extensions: 123 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 120 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 122 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 266270664 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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