BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG1014
(607 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 62 4e-10
At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 61 6e-10
At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 60 1e-09
At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 58 3e-09
At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 57 8e-09
At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 57 8e-09
At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 57 1e-08
At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 56 1e-08
At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 55 4e-08
At4g12110.1 68417.m01923 sterol desaturase family protein simila... 33 0.19
At4g22753.1 68417.m03284 sterol desaturase family protein simila... 32 0.34
At2g37700.1 68415.m04623 CER1 protein, putative similar to CER1 ... 31 0.45
At4g22756.1 68417.m03285 sterol desaturase family protein simila... 31 0.78
At1g02190.2 68414.m00150 CER1 protein, putative similar to CER1 ... 30 1.4
At1g02190.1 68414.m00149 CER1 protein, putative similar to CER1 ... 30 1.4
At5g46650.1 68418.m05748 zinc finger (C3HC4-type RING finger) fa... 28 5.5
At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase fam... 28 5.5
At1g02205.2 68414.m00154 CER1 protein identical to maize gl1 hom... 27 7.3
At1g02205.1 68414.m00153 CER1 protein identical to maize gl1 hom... 27 7.3
At5g41880.1 68418.m05099 DNA primase small subunit family contai... 27 9.6
At1g61080.1 68414.m06877 proline-rich family protein 27 9.6
At1g31780.1 68414.m03901 conserved oligomeric Golgi complex comp... 27 9.6
>At3g15870.1 68416.m02007 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 292
Score = 61.7 bits (143), Expect = 4e-10
Identities = 27/89 (30%), Positives = 46/89 (51%), Gaps = 2/89 (2%)
Frame = +2
Query: 335 WVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLR 514
WV +HR HHK+ +T DPH+ T+GF+FSH+ W+ + G+ ++ D+ P R
Sbjct: 104 WVSNHRYHHKHCETQRDPHSPTQGFWFSHMAWIFDTSSILENCGGEE-NVDDLVRQPFYR 162
Query: 515 FQKKYAIPFIGTICFIIPTLA--PMYFWG 595
F ++ + + F+ P+ WG
Sbjct: 163 FLQRTVLLHMMAYSFLFYFCGGMPLLVWG 191
>At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to
delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180
GI:2970034 from [Arabidopsis thaliana]
Length = 305
Score = 60.9 bits (141), Expect = 6e-10
Identities = 29/89 (32%), Positives = 49/89 (55%), Gaps = 2/89 (2%)
Frame = +2
Query: 335 WVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLR 514
WV HR HH++TD+D DPH+ GF+FSH+ WL + +V+ G+ ++ D+ +
Sbjct: 116 WVSTHRYHHQFTDSDRDPHSPNEGFWFSHLLWLFDTGY-LVEKCGRRTNVEDLKRQWYYK 174
Query: 515 FQKKYAIPFIGTICFIIPTLAPMYF--WG 595
F ++ + I T F++ + F WG
Sbjct: 175 FLQRTVLYHILTFGFLLYYFGGLSFLTWG 203
>At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to
delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from
[Arabidopsis thaliana]
Length = 307
Score = 59.7 bits (138), Expect = 1e-09
Identities = 30/89 (33%), Positives = 48/89 (53%), Gaps = 2/89 (2%)
Frame = +2
Query: 335 WVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLR 514
WV HR HH++TD++ DPH+ GF+FSH+ W+ + +V G+ ++ D+ R
Sbjct: 118 WVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWIYDSAY-LVSKCGRRANVEDLKRQWFYR 176
Query: 515 FQKKYAIPFIGTICFIIPTLAPMYF--WG 595
F +K + I + F + L M F WG
Sbjct: 177 FLQKTVLFHILGLGFFLFYLGGMSFVTWG 205
>At1g06360.1 68414.m00672 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 58.4 bits (135), Expect = 3e-09
Identities = 24/78 (30%), Positives = 43/78 (55%), Gaps = 2/78 (2%)
Frame = +2
Query: 335 WVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKS--LDMSDIYCNPL 508
WV HR HH++TD+D DPH+ GF+FSH+ W+ ++ K G++ +D+ +
Sbjct: 110 WVSIHRFHHQFTDSDRDPHSPIEGFWFSHVWWICDTRYIKYKCGGRNNVMDLKQQWFYWF 169
Query: 509 LRFQKKYAIPFIGTICFI 562
LR + + T+ ++
Sbjct: 170 LRMTIGFHVLMFWTVLYL 187
>At1g06100.1 68414.m00639 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 57.2 bits (132), Expect = 8e-09
Identities = 30/87 (34%), Positives = 44/87 (50%)
Frame = +2
Query: 335 WVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLR 514
WV HR HH++TD+D DPH+ GF+FSH+ W+ + K G+ ++ D+ R
Sbjct: 110 WVSIHRFHHQFTDSDRDPHSPIEGFWFSHVLWIFDTDYIREK-CGRRNNVMDLKQQWFYR 168
Query: 515 FQKKYAIPFIGTICFIIPTLAPMYFWG 595
F KK + I +I Y WG
Sbjct: 169 FLKKTLVLHILAFWTLI------YLWG 189
>At1g06090.1 68414.m00638 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]
Length = 299
Score = 57.2 bits (132), Expect = 8e-09
Identities = 30/87 (34%), Positives = 44/87 (50%)
Frame = +2
Query: 335 WVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLR 514
WV HR HH++TD+D DPH+ GF+FSH+ W+ + K G+ ++ D+ R
Sbjct: 110 WVSTHRFHHQFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYR 168
Query: 515 FQKKYAIPFIGTICFIIPTLAPMYFWG 595
F + IG TL +Y WG
Sbjct: 169 FLRN----TIGLHILTFWTL--VYLWG 189
>At1g06120.1 68414.m00641 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported
by cDNA:gi_12083275_gb_AF332434.1_AF332434
Length = 299
Score = 56.8 bits (131), Expect = 1e-08
Identities = 20/46 (43%), Positives = 29/46 (63%)
Frame = +2
Query: 335 WVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGK 472
WV HR HH++TD+D DPH+ GF+FSH+ W+ + K G+
Sbjct: 110 WVSTHRFHHQFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGR 155
>At3g15850.1 68416.m02005 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 371
Score = 56.4 bits (130), Expect = 1e-08
Identities = 27/89 (30%), Positives = 43/89 (48%), Gaps = 2/89 (2%)
Frame = +2
Query: 335 WVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLR 514
WV HR HH++ D+D DPH+ GF+FSH+ W+ + + + G+ ++ D+ P R
Sbjct: 181 WVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMF-DTNTITQRCGEPNNVGDLEKQPFYR 239
Query: 515 FQKKYAI--PFIGTICFIIPTLAPMYFWG 595
F + I P + P WG
Sbjct: 240 FLRTTYILHPLALAVALYAMGGFPFIVWG 268
>At1g06350.1 68414.m00671 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 300
Score = 54.8 bits (126), Expect = 4e-08
Identities = 27/78 (34%), Positives = 39/78 (50%), Gaps = 2/78 (2%)
Frame = +2
Query: 335 WVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKS--LDMSDIYCNPL 508
WV HR HH++TD+D DPH+ G FSHI W+ ++ K G+ LD+ +
Sbjct: 111 WVSIHRFHHQFTDSDRDPHSPKEGLLFSHILWIFDTQYIKYKCGGRDNVLDLKKQWFYKF 170
Query: 509 LRFQKKYAIPFIGTICFI 562
LR I TI ++
Sbjct: 171 LRRTIAVHILMFWTILYL 188
>At4g12110.1 68417.m01923 sterol desaturase family protein similar
to sterol 4-alpha-methyl-oxidase GI:16973471 from
[Arabidopsis thaliana]; contains Pfam profile PF01598:
Sterol desaturase
Length = 298
Score = 32.7 bits (71), Expect = 0.19
Identities = 14/38 (36%), Positives = 24/38 (63%)
Frame = +2
Query: 257 LLVYMDFISVSPQQNWLHRFLNTIFTWVRDHRLHHKYT 370
L+VY F+ W+HRF ++ + + + HR+HH+YT
Sbjct: 132 LVVY--FLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYT 167
>At4g22753.1 68417.m03284 sterol desaturase family protein similar
to sterol 4-alpha-methyl-oxidase GI:16973471 from
[Arabidopsis thaliana]; contains Pfam profile PF01598:
Sterol desaturase
Length = 291
Score = 31.9 bits (69), Expect = 0.34
Identities = 13/38 (34%), Positives = 24/38 (63%)
Frame = +2
Query: 257 LLVYMDFISVSPQQNWLHRFLNTIFTWVRDHRLHHKYT 370
L+VY F+ W+HR+++ + + + HR+HH+YT
Sbjct: 128 LVVY--FLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYT 163
>At2g37700.1 68415.m04623 CER1 protein, putative similar to CER1
GI:1199467 and maize gl1 homolog (glossy1 locus)
GI:1209703 from [Arabidopsis thaliana]; may be involved
in wax biosynthesis; contains a SUR2-type
hydroxylase/desaturase catalytic domain (PS50242)
Length = 493
Score = 31.5 bits (68), Expect = 0.45
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +2
Query: 302 WLHRFLNTIFTWVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMV 457
W HR L+ F + R H HH T +P + F HIG+ L+ P++
Sbjct: 140 WFHRALHHHFLYSRYHSHHHSSIVT--EPITSVVHPFAEHIGYTLILGLPLI 189
>At4g22756.1 68417.m03285 sterol desaturase family protein similar
to sterol 4-alpha-methyl-oxidase GI:16973471 from
[Arabidopsis thaliana]; contains Pfam profile PF01598:
Sterol desaturase
Length = 299
Score = 30.7 bits (66), Expect = 0.78
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 257 LLVYMDFISVSPQQNWLHRFLNTIFTWVRDHRLHHKYT 370
L+VY F+ W+HRF + + + + H +HH+YT
Sbjct: 132 LVVY--FLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYT 167
>At1g02190.2 68414.m00150 CER1 protein, putative similar to CER1
GI:1199467 and maize gl1 homolog (glossy1 locus)
GI:1209703 from [Arabidopsis thaliana]
Length = 623
Score = 29.9 bits (64), Expect = 1.4
Identities = 18/52 (34%), Positives = 24/52 (46%)
Frame = +2
Query: 302 WLHRFLNTIFTWVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMV 457
W HR L+ F + R H HH T +P + F HI + L+ PMV
Sbjct: 144 WFHRALHHHFLYSRYHSHHHSSIVT--EPITSVVHPFAEHIAYTLLFAIPMV 193
>At1g02190.1 68414.m00149 CER1 protein, putative similar to CER1
GI:1199467 and maize gl1 homolog (glossy1 locus)
GI:1209703 from [Arabidopsis thaliana]
Length = 627
Score = 29.9 bits (64), Expect = 1.4
Identities = 18/52 (34%), Positives = 24/52 (46%)
Frame = +2
Query: 302 WLHRFLNTIFTWVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMV 457
W HR L+ F + R H HH T +P + F HI + L+ PMV
Sbjct: 144 WFHRALHHHFLYSRYHSHHHSSIVT--EPITSVVHPFAEHIAYTLLFAIPMV 193
>At5g46650.1 68418.m05748 zinc finger (C3HC4-type RING finger)
family protein contains similarity to RING-H2 zinc
finger protein ATL6 [Arabidopsis thaliana] GI:4928403;
contains Pfam profile PF00097: Zinc finger, C3HC4 type
(RING finger)
Length = 289
Score = 27.9 bits (59), Expect = 5.5
Identities = 11/41 (26%), Positives = 19/41 (46%)
Frame = +1
Query: 217 IVVMNVIRFSYLHIAGLYGLYLCFTSAKLATSVFEHHFHMG 339
++++ VI I G + +Y C T + HH+H G
Sbjct: 26 VIILTVILLVVFFI-GFFAIYFCKCFYHTLTEAWNHHYHNG 65
>At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase family
protein low similarity to dTDP-D-glucose-4,6-dehydratase
from Aneurinibacillus thermoaerophilus GI:16357461,
Sphingomonas sp. GI:1314581; contains Pfam profile
PF01370: NAD dependent epimerase/dehydratase family;
putative NDP-rhamnose synthase (rhm2 gene) GI:31559258
Length = 667
Score = 27.9 bits (59), Expect = 5.5
Identities = 20/60 (33%), Positives = 26/60 (43%), Gaps = 4/60 (6%)
Frame = +2
Query: 371 DTDADPHN----ATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLRFQKKYAIP 538
DT P N GF SH+ L+R +P KI LD D YC+ L ++ P
Sbjct: 3 DTTYKPKNILITGAAGFIASHVANRLIRNYPDYKIV--VLDKLD-YCSDLKNLDPSFSSP 59
>At1g02205.2 68414.m00154 CER1 protein identical to maize gl1
homolog (glossy1 locus) GI:1209703 and CER1 GI:1199467
from [Arabidopsis thaliana]
Length = 625
Score = 27.5 bits (58), Expect = 7.3
Identities = 15/52 (28%), Positives = 25/52 (48%)
Frame = +2
Query: 302 WLHRFLNTIFTWVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMV 457
WLH+ L+ F + R H HH T +P + F HI + ++ P++
Sbjct: 144 WLHKALHHHFLYSRYHSHHHSSIVT--EPITSVIHPFAEHIAYFILFAIPLL 193
>At1g02205.1 68414.m00153 CER1 protein identical to maize gl1
homolog (glossy1 locus) GI:1209703 and CER1 GI:1199467
from [Arabidopsis thaliana]
Length = 580
Score = 27.5 bits (58), Expect = 7.3
Identities = 15/52 (28%), Positives = 25/52 (48%)
Frame = +2
Query: 302 WLHRFLNTIFTWVRDHRLHHKYTDTDADPHNATRGFFFSHIGWLLVRKHPMV 457
WLH+ L+ F + R H HH T +P + F HI + ++ P++
Sbjct: 144 WLHKALHHHFLYSRYHSHHHSSIVT--EPITSVIHPFAEHIAYFILFAIPLL 193
>At5g41880.1 68418.m05099 DNA primase small subunit family contains
Pfam profile: PF01896 DNA primase small subunit
Length = 407
Score = 27.1 bits (57), Expect = 9.6
Identities = 8/25 (32%), Positives = 16/25 (64%)
Frame = -2
Query: 249 IAKTYHVHHYNFVFDGRGLGCNEFF 175
I ++VH+ +DG+ GC+E++
Sbjct: 22 IENDFNVHYLRIYYDGKHPGCDEYY 46
>At1g61080.1 68414.m06877 proline-rich family protein
Length = 907
Score = 27.1 bits (57), Expect = 9.6
Identities = 19/54 (35%), Positives = 26/54 (48%)
Frame = +1
Query: 61 LNNL**VDKIEI*ITKMPPNSVDKTNETEYLKDNHVDYEKLIAPQASPIKHKIV 222
LN L +DK+E TK+ VD ++ L EKL++P A P K V
Sbjct: 810 LNQL--LDKVERYFTKIKETMVDISSNCMELALKEKRDEKLVSPDAKPSLKKTV 861
>At1g31780.1 68414.m03901 conserved oligomeric Golgi complex
component-related / COG complex component-related
similar to Conserved oligomeric Golgi complex component
6 (Swiss-Prot:Q9Y2V7) [Homo sapiens];
Length = 706
Score = 27.1 bits (57), Expect = 9.6
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = -3
Query: 521 SEILKEDYNKYLTCQGSFQQFSPLDVFAPTTSRCVR-KKNLS*HYEGLHQ 375
SE LK + L +GS +F + V + CVR K L+ YE ++Q
Sbjct: 628 SECLKAFFGLVLGSEGSLPEFEQIQVPKLRSEACVRVAKTLAEAYEVIYQ 677
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,706,634
Number of Sequences: 28952
Number of extensions: 257922
Number of successful extensions: 573
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 568
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 573
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1206913392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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