Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG1013
(653 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 127 7e-30
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 124 7e-29
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 120 8e-28
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 118 5e-27
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 35 0.054
At1g70330.1 68414.m08091 equilibrative nucleoside transporter fa... 31 0.67
At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK... 30 1.5
At5g38990.1 68418.m04717 protein kinase family protein contains ... 29 3.6
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim... 29 3.6
At3g46390.1 68416.m05029 hypothetical protein 28 4.7
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 28 4.7
At5g39000.1 68418.m04718 protein kinase family protein contains ... 27 8.2
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 27 8.2
At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containi... 27 8.2
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 127 bits (306), Expect = 7e-30
Identities = 77/200 (38%), Positives = 104/200 (52%), Gaps = 11/200 (5%)
Frame = +2
Query: 86 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-- 259
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + +V RW+N I
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDA 62
Query: 260 ---ASYTPAERK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXX-----VDLFGSGXXXXXX 412
S AE +G+SP+ VDLFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSSPITEEAVATPPAADSKDTAAEEEDDDDVDLFGEETEEEKK 122
Query: 413 XXXXXXXXXLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 592
+A + K S K KSS+L+D+KPWDDETDMK++E VR+I+MEGL WG
Sbjct: 123 AAEE------RAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWG 176
Query: 593 ASKLVPVGYGINKLQIMCVI 652
ASKLVPVGYGI KL IMC I
Sbjct: 177 ASKLVPVGYGIKKLHIMCTI 196
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 124 bits (298), Expect = 7e-29
Identities = 75/200 (37%), Positives = 104/200 (52%), Gaps = 11/200 (5%)
Frame = +2
Query: 86 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-- 259
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + + RWYN I
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDA 62
Query: 260 ---ASYTPAERK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXX-----VDLFGSGXXXXXX 412
S AE +G++P+ VDLFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSAPITEEAVATPPAADSKDAAADEEDDDDVDLFGEETEEEKK 122
Query: 413 XXXXXXXXXLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 592
+A + K S K KSS+L+D+KPWDDETDMK++E V++I+MEGL WG
Sbjct: 123 AAEE------RAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWG 176
Query: 593 ASKLVPVGYGINKLQIMCVI 652
ASKLVPVGYGI KLQI+C I
Sbjct: 177 ASKLVPVGYGIKKLQILCTI 196
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 120 bits (289), Expect = 8e-28
Identities = 71/189 (37%), Positives = 94/189 (49%), Gaps = 3/189 (1%)
Frame = +2
Query: 95 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTP 274
D+ T +G+ + ++LA K+Y+SG S DV+V+ V P+ P+ +WY +AS
Sbjct: 7 DLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVASQLA 66
Query: 275 AE---RKTWSQGTSPLXXXXXXXXXXXXXXXXXXXXVDLFGSGXXXXXXXXXXXXXXXLK 445
+ Q +DLFG +
Sbjct: 67 KSFPGKAVGVQFGGSAAAAPAVEAEAPAAAADDDDDMDLFGDETEEEKKAAEE------R 120
Query: 446 AYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPVGYGI 625
A K +KKP KSS+L+DVKPWDDETDMK++E VR +EM GL WGASKLVPVGYGI
Sbjct: 121 EAAKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPVGYGI 180
Query: 626 NKLQIMCVI 652
KL IM I
Sbjct: 181 KKLTIMFTI 189
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 118 bits (283), Expect = 5e-27
Identities = 71/193 (36%), Positives = 96/193 (49%), Gaps = 7/193 (3%)
Frame = +2
Query: 95 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTP 274
D+ T +GL L ++LA K+Y+SG S DV+V+ V + P P+ +WY+ +AS+
Sbjct: 7 DLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASHLA 66
Query: 275 AERKTWSQGTS-------PLXXXXXXXXXXXXXXXXXXXXVDLFGSGXXXXXXXXXXXXX 433
+ G P +DLF
Sbjct: 67 KSFPGKADGVRVGGGVAPPSEAHPHTEEPAADGDGDDDDDIDLFADETEDEKKAAEE--- 123
Query: 434 XXLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGASKLVPV 613
+ A K +KK KSS+LL+VKPWDDETDMK++E VR+++M GL WGASKLVPV
Sbjct: 124 ---REAAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVPV 180
Query: 614 GYGINKLQIMCVI 652
GYGI KL IM I
Sbjct: 181 GYGIKKLTIMMTI 193
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 34.7 bits (76), Expect = 0.054
Identities = 23/57 (40%), Positives = 29/57 (50%), Gaps = 9/57 (15%)
Frame = +2
Query: 116 LNDLNQYLAEKSYV--SGYTPSQADVQVFEQV-------GKAPAANLPHVLRWYNQI 259
L LN LA KS + +G TPS ADV VF + + +PHV+RW N I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At1g70330.1 68414.m08091 equilibrative nucleoside transporter
family protein contains similarity to SWISS-PROT:Q14542
equilibrative nucleoside transporter 2 (Equilibrative
nitrobenzylmercaptopurine riboside-insensitive
nucleoside transporter, Equilibrative NBMPR-insensitive
nucleoside transporter, Nucleoside transporter, ei-type,
36 kDa nucleolar protein HNP36, Hydrophobic nucleolar
protein, 36 kDa, Delayed-early response protein 12)
[Homo sapiens]
Length = 450
Score = 31.1 bits (67), Expect = 0.67
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = -2
Query: 592 SPEKAFHFNSAYLVFHFLHIGFIIPW 515
+P ++HF AY+++ L +GF++PW
Sbjct: 56 APSDSYHF--AYIIYFTLGVGFLLPW 79
>At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK,
putative similar to cyclin dependent kinase C
[Lycopersicon esculentum] gi|15215944|emb|CAC51391
Length = 513
Score = 29.9 bits (64), Expect = 1.5
Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = +2
Query: 131 QYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLR--WYNQIASYTPAERK 286
+ L K + G T ++ +++E G +N P V + WYNQ+ S P +R+
Sbjct: 233 ELLNGKPILPGKTENEQLNKIYELCGSPDESNWPGVSKMPWYNQMKSSRPLKRR 286
>At5g38990.1 68418.m04717 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 880
Score = 28.7 bits (61), Expect = 3.6
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +3
Query: 228 FPTYYDGIIKLLHTLRLNAKLGLRAPAH*PPVLNPRLPPQ 347
+PTYYD I+ + L+L+ G A + P L+P PPQ
Sbjct: 387 YPTYYDAILSGVEILKLSNSDGNLAGLNPIPQLSP--PPQ 424
>At5g08415.1 68418.m00991 lipoic acid synthase family protein
similar to lipoic acid synthase from Arabidopsis
thaliana [gi:3928758], from Mus musculus [gi:14669826]
Pfam profile PF04055: radical SAM domain protein
Length = 394
Score = 28.7 bits (61), Expect = 3.6
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +2
Query: 461 KSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 592
K KP +I K+SI+L + D+E +KE +R I+++ L G
Sbjct: 287 KISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328
>At3g46390.1 68416.m05029 hypothetical protein
Length = 69
Score = 28.3 bits (60), Expect = 4.7
Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = +2
Query: 440 LKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWGA--SKLVPV 613
+K Y+ K K+ + +I +D+K +M + E + I M G++W S L+ V
Sbjct: 1 MKRYSTKHFKRILIANNVNIDIDLKTGIVAKNMAKKEKIEQVISMGGIMWEGLNSSLIKV 60
Query: 614 GYGINKLQI 640
+ K QI
Sbjct: 61 DEALLKQQI 69
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 28.3 bits (60), Expect = 4.7
Identities = 16/32 (50%), Positives = 17/32 (53%)
Frame = +2
Query: 116 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGK 211
L DL Y YVS PSQA V + EQ GK
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGK 525
>At5g39000.1 68418.m04718 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 873
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/56 (25%), Positives = 28/56 (50%)
Frame = +3
Query: 231 PTYYDGIIKLLHTLRLNAKLGLRAPAH*PPVLNPRLPPQQRKTTMTTTLIYLVLVT 398
P YYD I+ + L++N G A + P+++P L P + + +++ +T
Sbjct: 390 PKYYDAILNGVEILKMNDPDGNLAGPNPDPLVSPDLIPNRATPRIRKNKSHILPIT 445
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 27.5 bits (58), Expect = 8.2
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 116 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANL 229
+N+ + + ++ V G TP+QADV+V VG A +
Sbjct: 386 MNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEI 423
>At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 617
Score = 27.5 bits (58), Expect = 8.2
Identities = 9/36 (25%), Positives = 18/36 (50%)
Frame = +2
Query: 482 IAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLW 589
+ S++L D+K WD+ M+ + ++ G W
Sbjct: 535 VISSNVLADMKMWDESAKMRALMRDRGVVKTPGCSW 570
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,905,386
Number of Sequences: 28952
Number of extensions: 272098
Number of successful extensions: 777
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 755
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 776
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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