Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0985
(528 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g50170.1 68416.m05485 hypothetical protein contains Pfam prof... 31 0.63
At3g20720.1 68416.m02622 expressed protein 29 2.6
At5g43840.1 68418.m05360 heat shock transcription factor family ... 28 3.4
At3g56990.1 68416.m06344 glycine-rich protein conserved hypothet... 28 3.4
At3g10540.1 68416.m01265 3-phosphoinositide-dependent protein ki... 28 3.4
At1g30420.1 68414.m03718 ATP-binding cassette transport protein,... 28 3.4
At1g69920.1 68414.m08046 glutathione S-transferase, putative sim... 28 4.5
At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA li... 28 4.5
At1g80750.1 68414.m09474 60S ribosomal protein L7 (RPL7A) simila... 27 5.9
At5g04510.2 68418.m00450 3-phosphoinositide-dependent protein ki... 27 7.8
At5g04510.1 68418.m00451 3-phosphoinositide-dependent protein ki... 27 7.8
At1g12550.1 68414.m01455 oxidoreductase family protein similar t... 27 7.8
At1g05150.1 68414.m00518 calcium-binding EF hand family protein ... 27 7.8
>At3g50170.1 68416.m05485 hypothetical protein contains Pfam profile
PF03140: Plant protein of unknown function
Length = 541
Score = 30.7 bits (66), Expect = 0.63
Identities = 16/31 (51%), Positives = 21/31 (67%)
Frame = +1
Query: 190 TPNVQNLLKRLTRPDFTKVFRKRNNQGLHAL 282
TPN ++LLKRLTR T+V KR Q +H +
Sbjct: 333 TPNTRSLLKRLTRN--TRVVDKRQQQLVHCV 361
>At3g20720.1 68416.m02622 expressed protein
Length = 1083
Score = 28.7 bits (61), Expect = 2.6
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +2
Query: 281 LKLPNISFSRKKSLNWKLLKLMKKPMCF 364
L + N+SF+ +L +LL L K P CF
Sbjct: 468 LHIENLSFAESPTLKVRLLNLEKDPACF 495
>At5g43840.1 68418.m05360 heat shock transcription factor family
protein contains Pfam profile: PF00447 HSF-type
DNA-binding domain
Length = 282
Score = 28.3 bits (60), Expect = 3.4
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +1
Query: 208 LLKRLTRPDFTKVFRKRNNQGL 273
LLK++ +P F + RKRN QG+
Sbjct: 173 LLKKIKKPSFLQSLRKRNLQGI 194
>At3g56990.1 68416.m06344 glycine-rich protein conserved
hypothetical protein SPCC330.09 - Schizosaccharomyces
pombe, PIR:T41319
Length = 711
Score = 28.3 bits (60), Expect = 3.4
Identities = 19/63 (30%), Positives = 32/63 (50%)
Frame = +1
Query: 136 NIVPSFYDGEQDPAPKFFTPNVQNLLKRLTRPDFTKVFRKRNNQGLHALKTPKYKFLTKE 315
+++PS++ E PAPK+ +P ++NL + L T ++ YKFL E
Sbjct: 341 SLIPSYFIPELGPAPKWCSP-LENLTEELEESAQTTIY-------------DNYKFLAME 386
Query: 316 ELE 324
+LE
Sbjct: 387 DLE 389
>At3g10540.1 68416.m01265 3-phosphoinositide-dependent protein
kinase, putative similar to 3-phosphoinositide-dependent
protein kinase-1 [Oryza sativa] gi|5001830|gb|AAD37166
Length = 486
Score = 28.3 bits (60), Expect = 3.4
Identities = 25/82 (30%), Positives = 39/82 (47%), Gaps = 2/82 (2%)
Frame = +1
Query: 235 FTKVFR--KRNNQGLHALKTPKYKFLTKEELELEIAKANEKADVLLHIPPVLKIHKPIDE 408
++KV R K++N ++ALK KF+TKE + D L H P ++K+ +
Sbjct: 56 YSKVVRAKKKDNGTVYALKIMDKKFITKENKTAYVKLERIVLDQLEH-PGIVKLFFTFQD 114
Query: 409 VLSRDPALIGYDTSKYLFTDIT 474
S AL + + LF IT
Sbjct: 115 TQSLYMALESCEGGE-LFDQIT 135
>At1g30420.1 68414.m03718 ATP-binding cassette transport protein,
putative contains Pfam profiles PF00005: ABC
transporter, PF00664: ABC transporter transmembrane
region
Length = 1488
Score = 28.3 bits (60), Expect = 3.4
Identities = 13/43 (30%), Positives = 24/43 (55%)
Frame = +1
Query: 355 DVLLHIPPVLKIHKPIDEVLSRDPALIGYDTSKYLFTDITFGI 483
D+ + PV+ H + ++ DPA +GY + +F +TFG+
Sbjct: 314 DLSQFVGPVILSHI-LQSMIEGDPAWVGYVYAFLIFFGVTFGV 355
>At1g69920.1 68414.m08046 glutathione S-transferase, putative
similar to glutathione transferase GB:CAA09188
[Alopecurus myosuroides]; supported by cDNA gi:15451157
gb:AY050343.
Length = 254
Score = 27.9 bits (59), Expect = 4.5
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 242 KFSARETIKVYMPLKLPNISFSRKK 316
KF A E +K YMP I F++KK
Sbjct: 227 KFRAHEAVKPYMPTVAEFIEFAKKK 251
>At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA
ligase (VALRS) nearly identical to SP|P93736 Valyl-tRNA
synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (ValRS)
{Arabidopsis thaliana}
Length = 1108
Score = 27.9 bits (59), Expect = 4.5
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = -1
Query: 195 WCKKLWSRVLFAIVK 151
WC KLW+ V FA++K
Sbjct: 782 WCNKLWNAVRFAMMK 796
>At1g80750.1 68414.m09474 60S ribosomal protein L7 (RPL7A) similar
to ribosomal protein L7 GB:AAA03081 GI:307388 from [Homo
sapiens]
Length = 247
Score = 27.5 bits (58), Expect = 5.9
Identities = 14/43 (32%), Positives = 25/43 (58%)
Frame = +1
Query: 292 KYKFLTKEELELEIAKANEKADVLLHIPPVLKIHKPIDEVLSR 420
++K L E+L EIA+ + ++ LK++KP+ +VL R
Sbjct: 179 EHKILGIEDLVNEIARVGDHFREVMRFLGPLKLNKPVADVLHR 221
>At5g04510.2 68418.m00450 3-phosphoinositide-dependent protein
kinase, putative similar to 3-phosphoinositide-dependent
protein kinase-1 [Oryza sativa] gi|5001830|gb|AAD37166
Length = 408
Score = 27.1 bits (57), Expect = 7.8
Identities = 24/82 (29%), Positives = 38/82 (46%), Gaps = 2/82 (2%)
Frame = +1
Query: 235 FTKVFR--KRNNQGLHALKTPKYKFLTKEELELEIAKANEKADVLLHIPPVLKIHKPIDE 408
++KV R K+ ++ALK KF+TKE + D L H P ++K++ +
Sbjct: 55 YSKVVRAKKKETGTVYALKIMDKKFITKENKTAYVKLERIVLDQLEH-PGIIKLYFTFQD 113
Query: 409 VLSRDPALIGYDTSKYLFTDIT 474
S AL + + LF IT
Sbjct: 114 TSSLYMALESCEGGE-LFDQIT 134
>At5g04510.1 68418.m00451 3-phosphoinositide-dependent protein
kinase, putative similar to 3-phosphoinositide-dependent
protein kinase-1 [Oryza sativa] gi|5001830|gb|AAD37166
Length = 491
Score = 27.1 bits (57), Expect = 7.8
Identities = 24/82 (29%), Positives = 38/82 (46%), Gaps = 2/82 (2%)
Frame = +1
Query: 235 FTKVFR--KRNNQGLHALKTPKYKFLTKEELELEIAKANEKADVLLHIPPVLKIHKPIDE 408
++KV R K+ ++ALK KF+TKE + D L H P ++K++ +
Sbjct: 55 YSKVVRAKKKETGTVYALKIMDKKFITKENKTAYVKLERIVLDQLEH-PGIIKLYFTFQD 113
Query: 409 VLSRDPALIGYDTSKYLFTDIT 474
S AL + + LF IT
Sbjct: 114 TSSLYMALESCEGGE-LFDQIT 134
>At1g12550.1 68414.m01455 oxidoreductase family protein similar to
glyoxylate reductase from Homo sapiens (gi:6002730);
contains Pfam D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain PF02826
Length = 323
Score = 27.1 bits (57), Expect = 7.8
Identities = 16/40 (40%), Positives = 25/40 (62%)
Frame = +1
Query: 331 IAKANEKADVLLHIPPVLKIHKPIDEVLSRDPALIGYDTS 450
+A+++E VLLH PP L +DE+L+R+ + DTS
Sbjct: 1 MAESSEPPVVLLHRPPSLTF---MDEILTREFRTLITDTS 37
>At1g05150.1 68414.m00518 calcium-binding EF hand family protein low
similarity to O-linked GlcNAc transferase [Homo sapiens]
GI:2266994; contains Pfam profiles PF00036: EF hand,
PF00515: TPR Domain
Length = 808
Score = 27.1 bits (57), Expect = 7.8
Identities = 17/52 (32%), Positives = 25/52 (48%), Gaps = 3/52 (5%)
Frame = +1
Query: 136 NIVPSFYDGEQDPAPKFFTPNVQNLLKRL---TRPDFTKVFRKRNNQGLHAL 282
N VP Y G P PN++ +L+RL +PD + K N+ + AL
Sbjct: 560 NDVPVSYSGSGGPTKSIRKPNLEEILRRLLSSLKPDTFQGAIKAINEKILAL 611
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,342,771
Number of Sequences: 28952
Number of extensions: 237498
Number of successful extensions: 505
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 496
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 505
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 977150592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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