Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0982
(575 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51840.1 68416.m05685 short-chain acyl-CoA oxidase identical ... 54 7e-08
At3g45300.1 68416.m04891 isovaleryl-CoA-dehydrogenase (IVD) iden... 51 5e-07
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 37 0.008
At3g55160.1 68416.m06126 expressed protein 32 0.32
At4g23060.1 68417.m03325 calmodulin-binding family protein conta... 28 3.9
At2g32850.2 68415.m04025 protein kinase family protein contains ... 28 3.9
At2g32850.1 68415.m04024 protein kinase family protein contains ... 28 3.9
At4g37120.1 68417.m05257 expressed protein 28 5.1
At5g33200.1 68418.m03919 hypothetical protein predicted replicat... 27 6.8
At4g34440.1 68417.m04894 protein kinase family protein contains ... 27 6.8
At1g01320.1 68414.m00048 tetratricopeptide repeat (TPR)-containi... 27 9.0
>At3g51840.1 68416.m05685 short-chain acyl-CoA oxidase identical to
Short-chain acyl CoA oxidase [Arabidopsis thaliana]
GI:5478795; contains InterPro entry IPR006089: Acyl-CoA
dehydrogenase
Length = 436
Score = 54.0 bits (124), Expect = 7e-08
Identities = 33/125 (26%), Positives = 59/125 (47%), Gaps = 1/125 (0%)
Frame = +1
Query: 199 LTDEQKALQELARKFTKDEIIPVAGQYDKTGEYPWPVVKKAWEVGLMNGHIPEHCGGIGN 378
LT E++A+++ R+ + E+ P+ +Y + E+P+ + K +G+ G I + G
Sbjct: 55 LTPEEQAIRKKVRECMEKEVAPIMTEYWEKAEFPFHITPKLGAMGVAGGSIKGY--GCPG 112
Query: 379 FGVFEECIVADELA-FXXXXXXXXXXXXSLGQTPIIIAGNKEQQKKYLGRLIDEPLVAAY 555
+ I E+A SLG I + G++ Q++KYL L VA +
Sbjct: 113 LSITANAIATAEIARVDASCSTFILVHSSLGMLTIALCGSEAQKEKYLPSLAQLNTVACW 172
Query: 556 GVTEP 570
+TEP
Sbjct: 173 ALTEP 177
>At3g45300.1 68416.m04891 isovaleryl-CoA-dehydrogenase (IVD)
identical to isovaleryl-CoA-dehydrogenase precursor
[Arabidopsis thaliana] GI:5596622
Length = 409
Score = 51.2 bits (117), Expect = 5e-07
Identities = 40/127 (31%), Positives = 57/127 (44%), Gaps = 5/127 (3%)
Frame = +1
Query: 205 DEQKALQELARKFTKDEIIPVAGQYDKTGEYPWPVVKKAWEV-GLMNGH---IPEHCGGI 372
D Q +E KF +D I P A + DKT +P V W++ G N H PE GG+
Sbjct: 33 DTQLQFKESVSKFAQDNIAPHAERIDKTNSFPKDV--NLWKLMGEFNLHGITAPEEYGGL 90
Query: 373 GNFGVFEECIVADELAFXXXXXXXXXXXXS-LGQTPIIIAGNKEQQKKYLGRLIDEPLVA 549
G G CI +E++ S L ++ G Q++KYL +LI V
Sbjct: 91 G-LGYLYHCIAMEEISRASGSVALSYGAHSNLCINQLVRNGTAAQKEKYLPKLISGEHVG 149
Query: 550 AYGVTEP 570
A ++EP
Sbjct: 150 ALAMSEP 156
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 37.1 bits (82), Expect = 0.008
Identities = 23/70 (32%), Positives = 31/70 (44%), Gaps = 2/70 (2%)
Frame = -1
Query: 467 PKDVPPIAVVMPVHPNASSSATMHSSKTPKF-PMPPQCSGMCPFIRPTSQAFFTTGHGYS 291
P P P P+ S ++ +S +P + P P S P PTS A+ T GYS
Sbjct: 1648 PAYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPGYS 1707
Query: 290 PVL-SY*PAT 264
P SY P +
Sbjct: 1708 PTSPSYSPTS 1717
Score = 34.3 bits (75), Expect = 0.059
Identities = 22/70 (31%), Positives = 30/70 (42%), Gaps = 2/70 (2%)
Frame = -1
Query: 467 PKDVPPIAVVMPVHPNASSSATMHSSKTPKF-PMPPQCSGMCPFIRPTSQAFFTTGHGYS 291
P P P P+ S ++ +S +P + P P S P PTS A+ T YS
Sbjct: 1599 PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSPAYS 1658
Query: 290 PVL-SY*PAT 264
P SY P +
Sbjct: 1659 PTSPSYSPTS 1668
Score = 34.3 bits (75), Expect = 0.059
Identities = 20/73 (27%), Positives = 32/73 (43%), Gaps = 1/73 (1%)
Frame = -1
Query: 467 PKDVPPIAVVMPVHPNASSSATMHSSKTPKF-PMPPQCSGMCPFIRPTSQAFFTTGHGYS 291
P P P P+ S ++ +S +P + P P S P PTS ++ YS
Sbjct: 1676 PSYSPTSPSYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYS 1735
Query: 290 PVLSY*PATGMIS 252
P ++Y P+ +S
Sbjct: 1736 PSIAYSPSNARLS 1748
Score = 32.3 bits (70), Expect = 0.24
Identities = 21/70 (30%), Positives = 30/70 (42%), Gaps = 2/70 (2%)
Frame = -1
Query: 467 PKDVPPIAVVMPVHPNASSSATMHSSKTPKF-PMPPQCSGMCPFIRPTSQAFFTTGHGYS 291
P P P P+ S ++ +S +P + P P S P PTS ++ T YS
Sbjct: 1613 PSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPTSPSYSPTSPSYS 1672
Query: 290 PVL-SY*PAT 264
P SY P +
Sbjct: 1673 PTSPSYSPTS 1682
Score = 31.5 bits (68), Expect = 0.42
Identities = 19/59 (32%), Positives = 27/59 (45%), Gaps = 2/59 (3%)
Frame = -1
Query: 434 PVHPNASSSATMHSSKTPKF-PMPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PAT 264
P P S ++ +S +P + P P S P PTS ++ T YSP SY P +
Sbjct: 1568 PTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTS 1626
Score = 27.9 bits (59), Expect = 5.1
Identities = 16/47 (34%), Positives = 22/47 (46%), Gaps = 1/47 (2%)
Frame = -1
Query: 425 PNASSSATMHSSKTPKF-PMPPQCSGMCPFIRPTSQAFFTTGHGYSP 288
P S S+ +S +P + P P S P PTS + + GYSP
Sbjct: 1543 PGYSPSSPGYSPTSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSP 1589
>At3g55160.1 68416.m06126 expressed protein
Length = 2149
Score = 31.9 bits (69), Expect = 0.32
Identities = 17/33 (51%), Positives = 24/33 (72%)
Frame = +2
Query: 407 RTNLRLDVLASRQLLGARP*DKLPSLLLEIRSS 505
++NLR+ VLASR L+G +KL S+LL I S+
Sbjct: 1395 QSNLRVRVLASRALVGLVSNEKLQSVLLRIAST 1427
>At4g23060.1 68417.m03325 calmodulin-binding family protein contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 543
Score = 28.3 bits (60), Expect = 3.9
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Frame = -2
Query: 427 IQTQVRPLRCTLRRRQNSLCPRNVLECVRSLGQLPK-PSLQRATGTPPSYHIDPPQ 263
++T R L+RR + + + E + Q+P PSL +T PPS+H P+
Sbjct: 26 VETPSRSTSSNLKRRWSFVKSKREKEST-PINQVPHTPSLPNSTPPPPSHHQSSPR 80
>At2g32850.2 68415.m04025 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 670
Score = 28.3 bits (60), Expect = 3.9
Identities = 25/72 (34%), Positives = 31/72 (43%), Gaps = 1/72 (1%)
Frame = -1
Query: 491 PAIMMGVCPKDVPPIAVVMPVHPNASSSATMHSSKTPKF-PMPPQCSGMCPFIRPTSQAF 315
PA + P D PP VH +S SAT S + P PP SG P AF
Sbjct: 299 PANLQKSLP-DRPPEMQSTGVHDGSSKSATKPSPAPRRSPPPPPPSSGESDSGGPLG-AF 356
Query: 314 FTTGHGYSPVLS 279
+ T H + V+S
Sbjct: 357 WATQHAKTSVVS 368
>At2g32850.1 68415.m04024 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 650
Score = 28.3 bits (60), Expect = 3.9
Identities = 25/72 (34%), Positives = 31/72 (43%), Gaps = 1/72 (1%)
Frame = -1
Query: 491 PAIMMGVCPKDVPPIAVVMPVHPNASSSATMHSSKTPKF-PMPPQCSGMCPFIRPTSQAF 315
PA + P D PP VH +S SAT S + P PP SG P AF
Sbjct: 299 PANLQKSLP-DRPPEMQSTGVHDGSSKSATKPSPAPRRSPPPPPPSSGESDSGGPLG-AF 356
Query: 314 FTTGHGYSPVLS 279
+ T H + V+S
Sbjct: 357 WATQHAKTSVVS 368
>At4g37120.1 68417.m05257 expressed protein
Length = 536
Score = 27.9 bits (59), Expect = 5.1
Identities = 12/33 (36%), Positives = 16/33 (48%), Gaps = 2/33 (6%)
Frame = +3
Query: 396 VHRSGRTCVWMYWHHDSYWGH--VLRTNSHHYC 488
VH + T VW W D WG+ +T + YC
Sbjct: 394 VHANNHTSVWGSWWKDHQWGYKCCQQTIRNSYC 426
>At5g33200.1 68418.m03919 hypothetical protein predicted replication
A1 proteins - Arabidopsis thaliana
Length = 426
Score = 27.5 bits (58), Expect = 6.8
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = +3
Query: 330 WPNERTHSRTLRGHREFWRL 389
+P TH RT+ ++EFW+L
Sbjct: 53 YPGTLTHLRTMEHNKEFWKL 72
>At4g34440.1 68417.m04894 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 670
Score = 27.5 bits (58), Expect = 6.8
Identities = 17/56 (30%), Positives = 22/56 (39%), Gaps = 2/56 (3%)
Frame = -1
Query: 491 PAIMMGVCPKDVPPIAVVMP-VHPNASSSATMHSS-KTPKFPMPPQCSGMCPFIRP 330
P I P PP P P++S + S +TP+ P PP G P P
Sbjct: 49 PDISASFSPPPAPPTQETSPPTSPSSSPPVVANPSPQTPENPSPPAPEGSTPVTPP 104
>At1g01320.1 68414.m00048 tetratricopeptide repeat (TPR)-containing
protein low similarity to SP|P46825 Kinesin light chain
(KLC) {Loligo pealeii}; contains Pfam profile PF00515:
TPR Domain
Length = 1797
Score = 27.1 bits (57), Expect = 9.0
Identities = 10/35 (28%), Positives = 17/35 (48%)
Frame = -1
Query: 467 PKDVPPIAVVMPVHPNASSSATMHSSKTPKFPMPP 363
P + P + M + P+ + T+H P +P PP
Sbjct: 1578 PSIIRPTPIGMNIGPSWPVNMTLHHGPPPPYPSPP 1612
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,279,828
Number of Sequences: 28952
Number of extensions: 258451
Number of successful extensions: 921
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 703
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 902
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1121903184
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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