BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0964 (659 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 93 2e-20 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 87 2e-18 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 66 6e-12 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 54 1e-08 SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 46 4e-06 SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 29 0.45 SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 28 1.0 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 1.4 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 3.2 SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 27 3.2 SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa... 26 4.2 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 26 5.5 SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 26 5.5 SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 25 7.3 SPAC31G5.10 |eta2||Myb family transcriptional regulator Eta2|Sch... 25 7.3 SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 25 9.7 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 93.5 bits (222), Expect = 2e-20 Identities = 51/134 (38%), Positives = 78/134 (58%), Gaps = 6/134 (4%) Frame = +1 Query: 247 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 414 + +Q+AEF+EAF L D D+DG I N+L SLG+ + EL +M+ E +G I+ Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66 Query: 415 FTQLLTLFANRMSGGSDEDDVVINAFKTFDEEGK--IDSERLRHALMTWGDKFSADEVDE 588 FT+ LT+ A +M +D ++ V AFK FD++G I E L H L + G++ S +EV + Sbjct: 67 FTEFLTMMARKMK-DTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVAD 125 Query: 589 AYDQMDIDDKGYID 630 + D D G I+ Sbjct: 126 MIREADTDGDGVIN 139 Score = 28.3 bits (60), Expect = 1.0 Identities = 19/53 (35%), Positives = 25/53 (47%), Gaps = 1/53 (1%) Frame = +1 Query: 502 DEEGKID-SERLRHALMTWGDKFSADEVDEAYDQMDIDDKGYIDTTKLIAMLT 657 D G ID +E L D + +EV EA+ D D GYI +L +LT Sbjct: 60 DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLT 112 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 87.0 bits (206), Expect = 2e-18 Identities = 47/130 (36%), Positives = 70/130 (53%), Gaps = 2/130 (1%) Frame = +1 Query: 202 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD 381 ++ AKR S F+ + Q+ E KEAF L+D D DG IG+ D++ SL + ASE ++ Sbjct: 28 AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87 Query: 382 EMVGEASGPINFTQLLTLFANRMSGGSDEDDVVINAFKTFD--EEGKIDSERLRHALMTW 555 M + PIN LT + + S +D ++ AF TFD + GKI +R AL + Sbjct: 88 HMFESINPPINLAAFLTAMGSMLCRISPRND-LLEAFSTFDDTQSGKIPISTMRDALSSM 146 Query: 556 GDKFSADEVD 585 GD+ EV+ Sbjct: 147 GDRMDPQEVE 156 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 65.7 bits (153), Expect = 6e-12 Identities = 39/130 (30%), Positives = 69/130 (53%), Gaps = 2/130 (1%) Frame = +1 Query: 247 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQL 426 S++Q E KEAF L D DKDG+I + + + SLG ++ EL ++ E I+ + Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63 Query: 427 LTLFANRMSGGSDEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQ 600 ++ +N++ E++ I AF+ FD++ G I++ + + T G+K S +EV + Sbjct: 64 MSFVSNKLRETESEEE-YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQE 122 Query: 601 MDIDDKGYID 630 D + G D Sbjct: 123 ADPTNSGSFD 132 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 54.4 bits (125), Expect = 1e-08 Identities = 36/142 (25%), Positives = 68/142 (47%), Gaps = 6/142 (4%) Frame = +1 Query: 247 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 414 +++Q + EAF+L D DKD I ++LRA +LG A + E+ +++ + G + Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91 Query: 415 FTQLLTLFANRMSGGSDEDDVVINAFKTF--DEEGKIDSERLRHALMTWGDKFSADEVDE 588 + + ++ D + + AF+ F DE GKI LR + E++ Sbjct: 92 MEDFVRVMTEKIV-ERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEA 150 Query: 589 AYDQMDIDDKGYIDTTKLIAML 654 ++ D+D G I+ + IA++ Sbjct: 151 MIEEFDLDQDGEINEQEFIAIM 172 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 46.4 bits (105), Expect = 4e-06 Identities = 33/133 (24%), Positives = 62/133 (46%), Gaps = 4/133 (3%) Frame = +1 Query: 268 FKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFTQLLTLFANR 447 +K+AF L D G I K + + G+ + E+ E+ ++ Q L + NR Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVL-NR 66 Query: 448 MSGGS--DEDDVVINAFKTFDEE--GKIDSERLRHALMTWGDKFSADEVDEAYDQMDIDD 615 +G + + + F+ FD++ G I LR+ L + G+K S +E+DE + + D Sbjct: 67 PNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD 126 Query: 616 KGYIDTTKLIAML 654 G ++ + M+ Sbjct: 127 -GMVNYHDFVQMI 138 Score = 40.7 bits (91), Expect = 2e-04 Identities = 18/42 (42%), Positives = 26/42 (61%) Frame = +1 Query: 265 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 390 EF + FQ+ D D G+IG +LR SLG S +E+DE++ Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119 >SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 29.5 bits (63), Expect = 0.45 Identities = 17/47 (36%), Positives = 26/47 (55%) Frame = -1 Query: 398 ASPTISSSSFSDASLPRESKVARKSFLPMMPSLSWSISWNASLNSAT 258 AS SSS S +++ V+ S P + + SISWN+S ++AT Sbjct: 126 ASNYTQSSSNSTSTMNSTGSVSGGSVYPTNSTTNSSISWNSSTSAAT 172 >SPAC1687.14c |||EF hand family protein, unknown role|Schizosaccharomyces pombe|chr 1|||Manual Length = 76 Score = 28.3 bits (60), Expect = 1.0 Identities = 10/19 (52%), Positives = 14/19 (73%) Frame = +1 Query: 574 DEVDEAYDQMDIDDKGYID 630 +E +EA+D D+ KGYID Sbjct: 12 EEAEEAFDLFDVTHKGYID 30 Score = 27.1 bits (57), Expect = 2.4 Identities = 15/42 (35%), Positives = 22/42 (52%) Frame = +1 Query: 265 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 390 E +EAF L D G I DLR + LG ++++L M+ Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 27.9 bits (59), Expect = 1.4 Identities = 17/46 (36%), Positives = 26/46 (56%) Frame = -1 Query: 395 SPTISSSSFSDASLPRESKVARKSFLPMMPSLSWSISWNASLNSAT 258 S +++SSS + +SL S + S P + S S+S A+ NSAT Sbjct: 146 SSSLASSSTTSSSLASSSTNSTTSATPTSSATSSSLSSTAASNSAT 191 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 26.6 bits (56), Expect = 3.2 Identities = 22/83 (26%), Positives = 35/83 (42%) Frame = -1 Query: 479 TTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMMPSL 300 +TSSSS L++ S +S ISSSS S +S S S Sbjct: 242 STSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTSSTISSSSSSSSSPT 301 Query: 299 SWSISWNASLNSATCFCENIEKT 231 S S + ++S +S++ F + + Sbjct: 302 STSSTISSSSSSSSSFSSTLSSS 324 >SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 882 Score = 26.6 bits (56), Expect = 3.2 Identities = 24/56 (42%), Positives = 31/56 (55%), Gaps = 2/56 (3%) Frame = -1 Query: 464 SEPPDMRLANRVK-S*VKL-MGPEASPTISSSSFSDASLPRESKVARKSFLPMMPS 303 S P R+A+ +K S VKL + P+A + SS+ S ASL ES R S P PS Sbjct: 542 SRPRLPRVASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSS--PTKPS 595 >SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 26.2 bits (55), Expect = 4.2 Identities = 23/63 (36%), Positives = 30/63 (47%) Frame = -1 Query: 488 ALITTSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMM 309 A I S SS+PP R S V+ A ++S+ FS P ES + S LP + Sbjct: 272 APIPFSVSSDPPSTM--GRSNSAVQSPSYFAHSLVNSTEFST---PNESLSSLPSILPSI 326 Query: 308 PSL 300 PSL Sbjct: 327 PSL 329 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 25.8 bits (54), Expect = 5.5 Identities = 21/73 (28%), Positives = 35/73 (47%) Frame = -1 Query: 476 TSSSSEPPDMRLANRVKS*VKLMGPEASPTISSSSFSDASLPRESKVARKSFLPMMPSLS 297 +SSSS ++ S +S + SSSS S +S S + S +P+ S S Sbjct: 156 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSVPITSSTS 215 Query: 296 WSISWNASLNSAT 258 S S ++S +S++ Sbjct: 216 SSHSSSSSSSSSS 228 >SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizosaccharomyces pombe|chr 3|||Manual Length = 457 Score = 25.8 bits (54), Expect = 5.5 Identities = 11/33 (33%), Positives = 22/33 (66%), Gaps = 1/33 (3%) Frame = -1 Query: 398 ASPTISSSSFSDASLPRESKVARKSF-LPMMPS 303 +SP++ +++ + P++S V+RKSF P P+ Sbjct: 35 SSPSVPEGTYTVLNNPKQSTVSRKSFSAPTSPT 67 >SPCC584.16c |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 231 Score = 25.4 bits (53), Expect = 7.3 Identities = 13/28 (46%), Positives = 16/28 (57%) Frame = -1 Query: 383 SSSSFSDASLPRESKVARKSFLPMMPSL 300 SSSS S +S P + +RK P MP L Sbjct: 3 SSSSSSGSSSPASKRSSRKRQTPPMPEL 30 >SPAC31G5.10 |eta2||Myb family transcriptional regulator Eta2|Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 25.4 bits (53), Expect = 7.3 Identities = 13/23 (56%), Positives = 17/23 (73%) Frame = +2 Query: 17 NSGSPGLQEFGTRVSSSSIVQHT 85 N+ LQEFG R+SSSS+ +HT Sbjct: 268 NNEGVDLQEFGFRMSSSSL-RHT 289 >SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1274 Score = 25.0 bits (52), Expect = 9.7 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +1 Query: 256 QVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASE 369 + A+FK+ F DKD + + +L A + L +LA + Sbjct: 212 EYAKFKDKFPEAPLDKDNLQTQKELEARIECLKQLAEK 249 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,965,212 Number of Sequences: 5004 Number of extensions: 31349 Number of successful extensions: 173 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 147 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 167 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 299817502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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