BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0919 (521 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 226 2e-60 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 226 2e-60 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 154 9e-39 SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 152 3e-38 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 152 3e-38 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 92 4e-20 SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 44 1e-05 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 42 4e-05 SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 41 1e-04 SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy... 27 1.7 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 5.2 SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccha... 25 6.8 SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha... 25 6.8 SPCC1753.02c |git3||G-protein coupled receptor Git3|Schizosaccha... 25 9.0 SPBC2F12.09c |atf21||transcription factor Atf21|Schizosaccharomy... 25 9.0 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 226 bits (552), Expect = 2e-60 Identities = 106/117 (90%), Positives = 110/117 (94%) Frame = +1 Query: 46 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 225 MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 226 IQKESTLHLVLRLRGGTIEPSLRILAMKYNCEKMICRKCYARLHPRATNCRKTKCTH 396 IQKESTLHLVLRLRGG IEPSL+ LA KYNCEK ICRKCYARL PRATNCRK KC H Sbjct: 61 IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGH 117 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 226 bits (552), Expect = 2e-60 Identities = 106/117 (90%), Positives = 110/117 (94%) Frame = +1 Query: 46 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 225 MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 226 IQKESTLHLVLRLRGGTIEPSLRILAMKYNCEKMICRKCYARLHPRATNCRKTKCTH 396 IQKESTLHLVLRLRGG IEPSL+ LA KYNCEK ICRKCYARL PRATNCRK KC H Sbjct: 61 IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRKKKCGH 117 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 154 bits (373), Expect = 9e-39 Identities = 77/92 (83%), Positives = 83/92 (90%), Gaps = 1/92 (1%) Frame = +1 Query: 1 LQEFGTRHVFHR-SNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLI 177 +Q+ T H+ R MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLI Sbjct: 61 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLI 120 Query: 178 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 273 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG Sbjct: 121 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152 Score = 154 bits (373), Expect = 9e-39 Identities = 77/92 (83%), Positives = 83/92 (90%), Gaps = 1/92 (1%) Frame = +1 Query: 1 LQEFGTRHVFHR-SNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLI 177 +Q+ T H+ R MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLI Sbjct: 137 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLI 196 Query: 178 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 273 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG Sbjct: 197 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 228 Score = 154 bits (373), Expect = 9e-39 Identities = 77/92 (83%), Positives = 83/92 (90%), Gaps = 1/92 (1%) Frame = +1 Query: 1 LQEFGTRHVFHR-SNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLI 177 +Q+ T H+ R MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLI Sbjct: 213 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLI 272 Query: 178 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 273 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG Sbjct: 273 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 304 Score = 154 bits (373), Expect = 9e-39 Identities = 77/92 (83%), Positives = 83/92 (90%), Gaps = 1/92 (1%) Frame = +1 Query: 1 LQEFGTRHVFHR-SNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLI 177 +Q+ T H+ R MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLI Sbjct: 289 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLI 348 Query: 178 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 273 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG Sbjct: 349 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 380 Score = 152 bits (369), Expect = 3e-38 Identities = 73/76 (96%), Positives = 76/76 (100%) Frame = +1 Query: 46 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 225 MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 226 IQKESTLHLVLRLRGG 273 IQKESTLHLVLRLRGG Sbjct: 61 IQKESTLHLVLRLRGG 76 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 152 bits (369), Expect = 3e-38 Identities = 73/76 (96%), Positives = 76/76 (100%) Frame = +1 Query: 46 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 225 MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 226 IQKESTLHLVLRLRGG 273 IQKESTLHLVLRLRGG Sbjct: 61 IQKESTLHLVLRLRGG 76 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 152 bits (369), Expect = 3e-38 Identities = 73/76 (96%), Positives = 76/76 (100%) Frame = +1 Query: 46 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 225 MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 226 IQKESTLHLVLRLRGG 273 IQKESTLHLVLRLRGG Sbjct: 61 IQKESTLHLVLRLRGG 76 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 92.3 bits (219), Expect = 4e-20 Identities = 40/77 (51%), Positives = 57/77 (74%) Frame = +1 Query: 46 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 225 M I VKTLTGK I L+++ +D + +K ++++KEGIPP QQRLI+AGKQ+ D + Y+ Sbjct: 1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYH 60 Query: 226 IQKESTLHLVLRLRGGT 276 ++ S LHLVL LRGG+ Sbjct: 61 LEGGSVLHLVLALRGGS 77 >SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 44.0 bits (99), Expect = 1e-05 Identities = 22/71 (30%), Positives = 42/71 (59%), Gaps = 1/71 (1%) Frame = +1 Query: 76 KTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKES-TLHL 252 K + +V A I +K KIQ ++ ++Q+LI++G+ L D +T+ +YNI+++ + + Sbjct: 12 KFVISDVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEYNIKEQDFIVCM 71 Query: 253 VLRLRGGTIEP 285 V R + T P Sbjct: 72 VSRPKTSTSTP 82 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 42.3 bits (95), Expect = 4e-05 Identities = 21/73 (28%), Positives = 39/73 (53%) Frame = +1 Query: 46 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 225 + + +K + + V++ ++ +K I I ++QRLI+AG+ L+D +L Y Sbjct: 4 ISLTIKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYK 63 Query: 226 IQKESTLHLVLRL 264 IQ ++HLV L Sbjct: 64 IQDGHSIHLVKTL 76 >SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 40.7 bits (91), Expect = 1e-04 Identities = 19/58 (32%), Positives = 33/58 (56%) Frame = +1 Query: 73 GKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTL 246 GK LE+E ++T +K ++ +PP++Q++I G QL+D L I+ +TL Sbjct: 10 GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATL 67 >SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces pombe|chr 2|||Manual Length = 603 Score = 27.1 bits (57), Expect = 1.7 Identities = 21/78 (26%), Positives = 36/78 (46%), Gaps = 2/78 (2%) Frame = +1 Query: 49 QIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSD--Y 222 +I V T+ K +V + T+ +K I P D+ +LI AG+ L + L + + Sbjct: 5 RIRVTTVDQKVGIFQVPRTKTVLELKELIAVTFEAPADRLKLIHAGRVLRNETPLEEILH 64 Query: 223 NIQKESTLHLVLRLRGGT 276 + T HLV+ + T Sbjct: 65 DATDLVTFHLVIAVFNST 82 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 25.4 bits (53), Expect = 5.2 Identities = 13/35 (37%), Positives = 21/35 (60%) Frame = -3 Query: 126 FDIFDSVGSFHLQCNGLPRKGFDEYLHCVAPMKNV 22 F ++S+G + L C + FDEYL+ A ++NV Sbjct: 2887 FKPYESLGKYMLSCLDDGKYAFDEYLY-HAEIQNV 2920 >SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccharomyces pombe|chr 2|||Manual Length = 485 Score = 25.0 bits (52), Expect = 6.8 Identities = 27/116 (23%), Positives = 48/116 (41%), Gaps = 13/116 (11%) Frame = +1 Query: 61 KTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFA------GKQLE-------D 201 K TGKT + + + ++ K+KIQ +P + L + GK + Sbjct: 89 KNGTGKTAAFVIPSLEKVDTKKSKIQTLILVPTRELALQTSQVCKTLGKHMNVKVMVTTG 148 Query: 202 GRTLSDYNIQKESTLHLVLRLRGGTIEPSLRILAMKYNCEKMICRKCYARLHPRAT 369 G TL D I+ T+H+V+ G ++ + + +A C + + L P T Sbjct: 149 GTTLRDDIIRLNDTVHIVVGTPGRVLDLAGKGVADFSECTTFVMDEADKLLSPEFT 204 >SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 541 Score = 25.0 bits (52), Expect = 6.8 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = -3 Query: 120 IFDSVGSFHLQCNGLPRKGFDEYL 49 IF VG+F C+ L ++GF E L Sbjct: 43 IFPGVGNFGFVCDSLAKQGFLEPL 66 >SPCC1753.02c |git3||G-protein coupled receptor Git3|Schizosaccharomyces pombe|chr 3|||Manual Length = 466 Score = 24.6 bits (51), Expect = 9.0 Identities = 10/33 (30%), Positives = 21/33 (63%) Frame = -1 Query: 131 LALTFSIVSEASTSNVMVFPVRVLTNICIVLLL 33 L TF++ STS++++ R L N+ I++++ Sbjct: 4 LDYTFNVSDATSTSSIIIVSRRELANLRIMVII 36 >SPBC2F12.09c |atf21||transcription factor Atf21|Schizosaccharomyces pombe|chr 2|||Manual Length = 355 Score = 24.6 bits (51), Expect = 9.0 Identities = 18/64 (28%), Positives = 25/64 (39%), Gaps = 3/64 (4%) Frame = +1 Query: 67 LTGKTITLEVEASDTIENVKAKIQDKEGIP---PDQQRLIFAGKQLEDGRTLSDYNIQKE 237 L G T EV T+ + + +G P P + I A +G TL+ YN Sbjct: 94 LKGPERTSEVSLPQTVNLSEISNNNDKGQPTNTPPVRSTIVAPSLYSEGSTLNHYNNGSH 153 Query: 238 STLH 249 LH Sbjct: 154 QVLH 157 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,218,730 Number of Sequences: 5004 Number of extensions: 45341 Number of successful extensions: 136 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 128 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 136 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 212331630 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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