Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0894
(538 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g20360.1 68415.m02377 expressed protein 120 8e-28
At1g19540.1 68414.m02434 isoflavone reductase, putative similar ... 36 0.017
At1g75290.1 68414.m08746 isoflavone reductase, putative similar ... 33 0.12
At1g75280.1 68414.m08745 isoflavone reductase, putative identica... 33 0.12
At2g44040.1 68415.m05475 dihydrodipicolinate reductase family pr... 32 0.21
At1g75300.1 68414.m08747 isoflavone reductase, putative identica... 32 0.21
At2g45310.1 68415.m05639 NAD-dependent epimerase/dehydratase fam... 31 0.64
At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondri... 29 1.5
At3g59890.2 68416.m06683 dihydrodipicolinate reductase family pr... 29 1.5
At3g59890.1 68416.m06684 dihydrodipicolinate reductase family pr... 29 1.5
At3g03980.1 68416.m00419 short-chain dehydrogenase/reductase (SD... 29 2.0
At2g34070.1 68415.m04171 expressed protein 29 2.6
At5g19060.1 68418.m02266 expressed protein ; expression supporte... 28 3.4
At4g11370.1 68417.m01833 zinc finger (C3HC4-type RING finger) fa... 28 3.4
At3g46810.1 68416.m05081 DC1 domain-containing protein contains ... 28 4.5
At1g02000.1 68414.m00118 NAD-dependent epimerase/dehydratase fam... 28 4.5
At5g54960.1 68418.m06845 pyruvate decarboxylase, putative strong... 27 7.9
At5g50310.1 68418.m06229 kelch repeat-containing protein similar... 27 7.9
At5g44400.1 68418.m05436 FAD-binding domain-containing protein s... 27 7.9
At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family pr... 27 7.9
At3g58660.1 68416.m06538 60S ribosomal protein-related contains ... 27 7.9
>At2g20360.1 68415.m02377 expressed protein
Length = 402
Score = 120 bits (288), Expect = 8e-28
Identities = 60/125 (48%), Positives = 90/125 (72%)
Frame = +3
Query: 159 HLNGSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYVCNKLG 338
HLNG+ + Y +++ ++ +LA ++GTGGRSS +GIVATVFG TGF+GRY+ +L
Sbjct: 34 HLNGTDNCRY--SSSLATKGVGHLA--RKGTGGRSSVSGIVATVFGATGFLGRYLVQQLA 89
Query: 339 KIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAKAVRYSNVVINLVGRDY 518
K+G+Q+++P+RG + LK+ GDLGQV+ + DE+SI + +NVVINL+GR+Y
Sbjct: 90 KMGSQVLVPFRGSEDSPRHLKLMGDLGQVVPMKFDPRDEDSIKAVMAKANVVINLIGREY 149
Query: 519 ETKNF 533
ET+NF
Sbjct: 150 ETRNF 154
>At1g19540.1 68414.m02434 isoflavone reductase, putative similar to
SP|P52577; contains isoflavone reductase domain PF02716
Length = 310
Score = 35.9 bits (79), Expect = 0.017
Identities = 26/79 (32%), Positives = 39/79 (49%), Gaps = 4/79 (5%)
Frame = +3
Query: 288 VFGCTGFVGRYVCNKLGKIGTQLILPYR----GDFYDAQRLKVCGDLGQVLFTPYHLLDE 455
V G TG +G+ + + K G R D AQ ++ DLG + L D+
Sbjct: 7 VIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYG-SLSDK 65
Query: 456 ESIAKAVRYSNVVINLVGR 512
ES+ KA++ +VVI+ VGR
Sbjct: 66 ESLVKAIKQVDVVISAVGR 84
>At1g75290.1 68414.m08746 isoflavone reductase, putative similar to
SP|P52577 Isoflavone reductase homolog P3 (EC 1.3.1.-)
{Arabidopsis thaliana}; contains Pfam profile PF02716:
Isoflavone reductase
Length = 323
Score = 33.1 bits (72), Expect = 0.12
Identities = 24/77 (31%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +3
Query: 288 VFGCTGFVGRYVCNKLGKIG-TQLILPYRGDFYDAQRLKVCGDLGQVLFTPYH--LLDEE 458
V G TG +G+ + K G + L L D + K + T H L D E
Sbjct: 10 VIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGDLNDHE 69
Query: 459 SIAKAVRYSNVVINLVG 509
S+ KA++ ++VVI+ VG
Sbjct: 70 SLVKAIKQADVVISTVG 86
>At1g75280.1 68414.m08745 isoflavone reductase, putative identical
to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: isoflavone reductase
Length = 310
Score = 33.1 bits (72), Expect = 0.12
Identities = 23/77 (29%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +3
Query: 288 VFGCTGFVGRYVCNKLGKIG-TQLILPYRGDFYDAQRLKVCGDLGQVLFTPYH--LLDEE 458
V G TG++G+++ K G + L D + K + T H L D E
Sbjct: 10 VIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLNDHE 69
Query: 459 SIAKAVRYSNVVINLVG 509
S+ KA++ +VVI+ VG
Sbjct: 70 SLVKAIKQVDVVISTVG 86
>At2g44040.1 68415.m05475 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae} ;
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 347
Score = 32.3 bits (70), Expect = 0.21
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +3
Query: 270 NGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYDAQRLKVCG 410
NGI V GC+G +G+ V G ++ + + D QR++VCG
Sbjct: 71 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPISFGSAGEDGQRVEVCG 118
>At1g75300.1 68414.m08747 isoflavone reductase, putative identical
to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: Isoflavone reductase
Length = 322
Score = 32.3 bits (70), Expect = 0.21
Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 4/78 (5%)
Frame = +3
Query: 288 VFGCTGFVGRYVCNKLGKIGTQLILPYR----GDFYDAQRLKVCGDLGQVLFTPYHLLDE 455
V G TG++G ++ K G R D ++ ++ DLG V L D
Sbjct: 10 VIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLG-VTILHGDLNDH 68
Query: 456 ESIAKAVRYSNVVINLVG 509
ES+ KA++ +VVI+ +G
Sbjct: 69 ESLVKAIKQVDVVISTIG 86
>At2g45310.1 68415.m05639 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam profile
PF01370 NAD dependent epimerase/dehydratase family
Length = 437
Score = 30.7 bits (66), Expect = 0.64
Identities = 18/43 (41%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +3
Query: 261 SSFNGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYD 386
S+ NGI V G GFVG +V L + G +I L D+YD
Sbjct: 92 STNNGITVLVTGAAGFVGTHVSAALKRRGDGVIGLDNFNDYYD 134
>At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondrial
/ ADP/ATP translocase 2 / adenine nucleotide
translocator 2 (ANT2) identical to SWISS-PROT:P40941
ADP,ATP carrier protein 2, mitochondrial precursor
(Adenine nucleotide translocator 2) [Arabidopsis
thaliana]
Length = 385
Score = 29.5 bits (63), Expect = 1.5
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 7/100 (7%)
Frame = +3
Query: 168 GSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIV---ATVFGCTGFVGRYVCNKLG 338
G+ S++++ + +Y+ R N + + GG FNG+V G G Y +
Sbjct: 200 GASSLLFVYSLDYARTRLANDSKSAKKGGGERQFNGLVDVYKKTLKSDGIAGLYRGFNIS 259
Query: 339 KIGTQLILPYRG---DFYDAQR-LKVCGDLGQVLFTPYHL 446
G I+ YRG YD+ + + + GDL F + L
Sbjct: 260 CAG---IIVYRGLYFGLYDSVKPVLLTGDLQDSFFASFAL 296
>At3g59890.2 68416.m06683 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae};
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 343
Score = 29.5 bits (63), Expect = 1.5
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +3
Query: 270 NGIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDA-QRLKVCG 410
NGI V GC+G +G+ V G ++ G +A Q ++VCG
Sbjct: 67 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPTSFGSVEEAGQTVEVCG 114
>At3g59890.1 68416.m06684 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae};
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 349
Score = 29.5 bits (63), Expect = 1.5
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +3
Query: 270 NGIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDA-QRLKVCG 410
NGI V GC+G +G+ V G ++ G +A Q ++VCG
Sbjct: 73 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPTSFGSVEEAGQTVEVCG 120
>At3g03980.1 68416.m00419 short-chain dehydrogenase/reductase (SDR)
family protein similar to short-chain type
dehydrogenase/reductase SP:Q08632 [Picea abies]
Length = 270
Score = 29.1 bits (62), Expect = 2.0
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = +3
Query: 273 GIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDAQRL 398
G VA V G + +GR + L ++G ++++ Y DA+R+
Sbjct: 16 GRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERV 57
>At2g34070.1 68415.m04171 expressed protein
Length = 385
Score = 28.7 bits (61), Expect = 2.6
Identities = 19/55 (34%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +3
Query: 162 LNGS-MSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYV 323
L+G+ ++ Y+K + S K LAA GG+ G T GC F GR+V
Sbjct: 20 LSGADQALAYVKKPHVSQRNKTALAAVAGRGGGKEMLKGRKQT-SGCNLFQGRWV 73
>At5g19060.1 68418.m02266 expressed protein ; expression supported
by MPSS
Length = 551
Score = 28.3 bits (60), Expect = 3.4
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -2
Query: 279 RCR*SYCGHQYLAYMLQGWVYDPNCS*RL 193
RCR +C A GWVY +CS +L
Sbjct: 272 RCREPWCDGALSALESNGWVYSSHCSFKL 300
>At4g11370.1 68417.m01833 zinc finger (C3HC4-type RING finger)
family protein strong similarity to RING-H2 finger
protein RHA1a [Arabidopsis thaliana] GI:3790554;
contains Pfam profile PF00097: Zinc finger, C3HC4 type
(RING finger)
Length = 159
Score = 28.3 bits (60), Expect = 3.4
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 2/81 (2%)
Frame = -2
Query: 444 DGKE*TGPGLNLRTLSTFGHHRNRLC-MVKLTGYQFFPI-CCTHSVRQIRCNRKLWLRCR 271
D E + P L LST N L +V+ + P CCT + + K+ +
Sbjct: 45 DHNETSAPDLTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPK 104
Query: 270 *SYCGHQYLAYMLQGWVYDPN 208
CGH + Y L W+ D N
Sbjct: 105 ---CGHVFHHYCLDRWIVDYN 122
>At3g46810.1 68416.m05081 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 686
Score = 27.9 bits (59), Expect = 4.5
Identities = 16/66 (24%), Positives = 28/66 (42%)
Frame = +3
Query: 303 GFVGRYVCNKLGKIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAKAVRY 482
GF G Y C+K + R D +D L+ G + P+ ++DE +I
Sbjct: 328 GFYGAYSCSKCSSYVVHSLCATRKDVWDMVELE--GTPEEEEIAPFEVVDENTIKHMSHD 385
Query: 483 SNVVIN 500
+++ N
Sbjct: 386 HDLIFN 391
>At1g02000.1 68414.m00118 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam profile
PF01370 NAD dependent epimerase/dehydratase family
Length = 434
Score = 27.9 bits (59), Expect = 4.5
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = +3
Query: 270 NGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYD 386
NG+ V G GFVG +V L + G ++ L D+YD
Sbjct: 90 NGVSVLVTGAAGFVGTHVSAALKRRGDGVLGLDNFNDYYD 129
>At5g54960.1 68418.m06845 pyruvate decarboxylase, putative strong
similarity to pyruvate decarboxylase 1 [Vitis vinifera]
GI:10732644; contains InterPro entry IPR000399: Pyruvate
decarboxylase
Length = 607
Score = 27.1 bits (57), Expect = 7.9
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = +3
Query: 285 TVFGCTGFVGRYVCNKLGKIGTQLILPYRGDF 380
TV C +GRY+ +L +IG + GDF
Sbjct: 39 TVSPCDATLGRYLARRLVEIGVTDVFSVPGDF 70
>At5g50310.1 68418.m06229 kelch repeat-containing protein similar to
Kelch repeats protein 3 (SP:Q08979) [Saccharomyces
cerevisiae]; contains Pfam PF01344: Kelch motif (6
repeats)
Length = 666
Score = 27.1 bits (57), Expect = 7.9
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +3
Query: 348 TQLILPYRGDFYDAQRLKVCGDL 416
T+LIL Y G+FY+ Q+ V GDL
Sbjct: 82 TELIL-YGGEFYNGQKTYVYGDL 103
>At5g44400.1 68418.m05436 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 537
Score = 27.1 bits (57), Expect = 7.9
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = -2
Query: 351 GYQFFPICCTHSVRQIRCNRKLWLRCR*SYCGHQY--LAYMLQ 229
G+ F PI +H I C++KL + R GH Y L+Y+ Q
Sbjct: 85 GFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQ 127
>At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain ;annotation temporarily based on
supporting cDNA gi|17224394|gb|AF246291.1|AF246291
Length = 637
Score = 27.1 bits (57), Expect = 7.9
Identities = 16/68 (23%), Positives = 29/68 (42%)
Frame = +3
Query: 168 GSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYVCNKLGKIG 347
G+M++ K + N A + G G + S N + TGF G ++G G
Sbjct: 506 GTMTMKRKKPCDAGERTSANCANNETGNGKKVSVNNVGEAEPADTGFTGLTDNLRIGSFG 565
Query: 348 TQLILPYR 371
++++ R
Sbjct: 566 NEVVIELR 573
>At3g58660.1 68416.m06538 60S ribosomal protein-related contains
weak similarity to 60S ribosomal protein L10A (CSA-19)
(NEDD-6) (Swiss-Prot:P53026) [Mus musculus]
Length = 446
Score = 27.1 bits (57), Expect = 7.9
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = +3
Query: 6 TRNIVYTLHTRIEFVHFKWEYYQLYK*ELSQYLS 107
T N++ TL+ +EF+ KW Y + +LS+ L+
Sbjct: 222 TENVMATLNGLVEFLPNKWTYVRSLHLKLSESLA 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,209,113
Number of Sequences: 28952
Number of extensions: 221410
Number of successful extensions: 531
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 504
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 531
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 993966856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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