BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0889 (560 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 72 5e-14 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 57 2e-09 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 56 4e-09 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 52 6e-08 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 47 2e-06 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 44 2e-05 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 32 0.050 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 30 0.27 SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 29 0.62 SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Sch... 28 1.1 SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 27 1.4 SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 25 5.8 SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 5.8 SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 25 7.6 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 72.1 bits (169), Expect = 5e-14 Identities = 34/90 (37%), Positives = 51/90 (56%) Frame = +2 Query: 107 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXX 286 + DS + K+ + + DKLVV+DF ATWCGPCK I PK ++ + Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQF-SNTYSDATFIKVDVDQLS 61 Query: 287 XXASEYNINSMPTFVFVKNGKKLDEFSGAN 376 A+E +++MP+F KNG+K++E GAN Sbjct: 62 EIAAEAGVHAMPSFFLYKNGEKIEEIVGAN 91 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 56.8 bits (131), Expect = 2e-09 Identities = 28/91 (30%), Positives = 47/91 (51%) Frame = +2 Query: 107 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXX 286 ++ D TR++ DK+ V+DF A WCGPCK + P L+++ +E Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKL-SEQNQKASFIAVNADKFS 78 Query: 287 XXASEYNINSMPTFVFVKNGKKLDEFSGANV 379 A + + ++PT V + G++LD GA+V Sbjct: 79 DIAQKNGVYALPTMVLFRKGQELDRIVGADV 109 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 56.0 bits (129), Expect = 4e-09 Identities = 26/73 (35%), Positives = 38/73 (52%), Gaps = 1/73 (1%) Frame = +2 Query: 161 VVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXX-ASEYNINSMPTFVFV 337 + +D A WCGPCK I P ++A++ AS + +MPTFVF Sbjct: 22 LAVDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFF 81 Query: 338 KNGKKLDEFSGAN 376 +NGK++D +GAN Sbjct: 82 ENGKQIDMLTGAN 94 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 52.0 bits (119), Expect = 6e-08 Identities = 24/74 (32%), Positives = 37/74 (50%), Gaps = 1/74 (1%) Frame = +2 Query: 152 DKLVVIDFMATWCGPCKMIGPKLDEIAAEM-XXXXXXXXXXXXXXXXXASEYNINSMPTF 328 DK++++ F A WCG CK + P+ + A E+ SEY+I PT Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTL 98 Query: 329 VFVKNGKKLDEFSG 370 KNGK++ ++SG Sbjct: 99 NVFKNGKQISQYSG 112 Score = 36.7 bits (81), Expect = 0.002 Identities = 19/68 (27%), Positives = 31/68 (45%) Frame = +2 Query: 155 KLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVF 334 K V+++F A WCG CK + P +++A E S +I+ PT +F Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATENDIS-VSISGFPTIMF 432 Query: 335 VKNGKKLD 358 K K++ Sbjct: 433 FKANDKVN 440 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 47.2 bits (107), Expect = 2e-06 Identities = 23/94 (24%), Positives = 42/94 (44%) Frame = +2 Query: 95 MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXX 274 MS+ I + + L +++++++F A W PCK + D+ A + Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKD-TKNAVFLKIEA 59 Query: 275 XXXXXXASEYNINSMPTFVFVKNGKKLDEFSGAN 376 A +++N++P FV + K L SGAN Sbjct: 60 EKFSDIAESFDVNAVPLFVLIHGAKVLARISGAN 93 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 44.0 bits (99), Expect = 2e-05 Identities = 28/108 (25%), Positives = 49/108 (45%), Gaps = 3/108 (2%) Frame = +2 Query: 59 VILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAA- 235 VI L ++ + ++ ++L+ + A K +I+F ATWCG CK + P +E+ A Sbjct: 9 VIFALFALVFASGVVELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGAL 67 Query: 236 -EMXXXXXXXXXXXXXXXXXASEYNINSMPTFV-FVKNGKKLDEFSGA 373 E A +Y+I PT + F +G + ++S A Sbjct: 68 FEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNA 115 Score = 30.3 bits (65), Expect = 0.20 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = +2 Query: 155 KLVVIDFMATWCGPCKMIGPKLDEI 229 K V+++F A WCG CK + P + + Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 32.3 bits (70), Expect = 0.050 Identities = 31/121 (25%), Positives = 46/121 (38%), Gaps = 2/121 (1%) Frame = +2 Query: 20 TLSLACFLFAF*TVILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPC 199 TL LACF +F S +I + +S + + + G LVV F A WCG C Sbjct: 10 TLFLACFSLVSG---VFGYSPMFGSNTIEL-NSKNFRKFVKAKGPSLVV--FYAPWCGYC 63 Query: 200 KMIGPKLDEIAAEMXXXXXXXXXXXXXXXXXA--SEYNINSMPTFVFVKNGKKLDEFSGA 373 K + P ++A+ + A S+Y + PT V K S Sbjct: 64 KKLVPTYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSST 123 Query: 374 N 376 + Sbjct: 124 D 124 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 29.9 bits (64), Expect = 0.27 Identities = 24/104 (23%), Positives = 44/104 (42%), Gaps = 3/104 (2%) Frame = +2 Query: 59 VILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLD---EI 229 V+ F+ + + + D+D L++ +++ I + CG CK +GP D E Sbjct: 15 VVAFITMVQTLVSGVPLTDND-LESEVSKG---TWFIKYYLPSCGACKRLGPMWDNMVEK 70 Query: 230 AAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVFVKNGKKLDE 361 A E +S NI ++PT +NG+ ++E Sbjct: 71 AKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLYLYQNGEIVEE 114 >SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 28.7 bits (61), Expect = 0.62 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -1 Query: 533 KFITDVKNIFYSFIKNVYMFFFFNSILVSI 444 KFI D +N F+ I Y F F ++L+ + Sbjct: 436 KFILDSQNFFFESINTEYSFIIFTNLLMHL 465 >SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 27.9 bits (59), Expect = 1.1 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = -2 Query: 538 SSNSLQTLKTFFIHLLKTYTCFFFLIP 458 SS +L +F+ L++ + CFFF+ P Sbjct: 50 SSGPFISLSFWFLSLVRGFVCFFFMFP 76 >SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 27.5 bits (58), Expect = 1.4 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = -1 Query: 557 QTQKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSIGGC 435 ++ + N ++TD +NIF +F+ N + F + L I C Sbjct: 71 KSSESNSIDYLTDTQNIFPNFVNNENNYQFSTAPLNPIDAC 111 >SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1029 Score = 25.4 bits (53), Expect = 5.8 Identities = 9/22 (40%), Positives = 16/22 (72%) Frame = -2 Query: 547 KLISSNSLQTLKTFFIHLLKTY 482 KL+SSN+LQ + F+ ++K + Sbjct: 412 KLVSSNTLQAMSHFYATMIKLF 433 >SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 676 Score = 25.4 bits (53), Expect = 5.8 Identities = 16/59 (27%), Positives = 30/59 (50%) Frame = -2 Query: 406 FKDSCFEFVDVSAREFVQFLAILNEDERRHRVNVVLAGDVLALINVHLHNDDGI*HFGG 230 F + +E+VD ++ + + F+ L + ++ GD + INV L + D +FGG Sbjct: 114 FSANLYEYVDGNS-DGISFVLNLENNNDTSVYHMTFHGDRVKPINVFLGSTDVTPNFGG 171 >SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 25.0 bits (52), Expect = 7.6 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = +2 Query: 113 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAA 235 + D + RL EAG + +A CGPC + + D+IA+ Sbjct: 431 ERDGITERLEEAGATV-----LANACGPCIGMWKRTDDIAS 466 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,985,910 Number of Sequences: 5004 Number of extensions: 35076 Number of successful extensions: 138 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 128 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 133 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 236012634 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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