BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0862 (593 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy... 179 2e-46 SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 33 0.042 SPCC330.05c |ura4||orotidine 5'-phosphate decarboxylase Ura4 |Sc... 30 0.29 SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 29 0.51 SPAC2G11.03c |vps45||vacuolar sorting protein Vps 45|Schizosacch... 29 0.51 SPBC405.03c |||membrane transporter |Schizosaccharomyces pombe|c... 27 2.7 SPBC660.14 |mik1||mitotic inhibitor kinase Mik1|Schizosaccharomy... 26 4.8 SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr 2||... 26 4.8 SPBC4B4.03 |rsc1||RSC complex subunit Rsc1 |Schizosaccharomyces ... 25 6.3 SPBC530.05 |||transcription factor |Schizosaccharomyces pombe|ch... 25 6.3 SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 25 6.3 SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 25 6.3 SPBC36.05c |clr6||histone deacetylase |Schizosaccharomyces pombe... 25 6.3 SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces... 25 6.3 SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3... 25 6.3 SPCC285.11 |ucp10||UBA/UAS domain protein Ucp10|Schizosaccharomy... 25 8.3 SPAC9E9.12c |ybt1|abc1|ABC transporter Ybt1|Schizosaccharomyces ... 25 8.3 SPCC1795.01c |mad3|SPCC895.02|mitotic spindle checkpoint protein... 25 8.3 >SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 754 Score = 179 bits (436), Expect = 2e-46 Identities = 90/177 (50%), Positives = 112/177 (63%), Gaps = 14/177 (7%) Frame = +1 Query: 103 FTNIAKQYEHEFWLDMASLNVAKPLIITRVSEHINSIEKFVQRLIDNDMAYVSKNGSVYF 282 F +A +E +F DMA+LNV P +TRVSE++ I FVQR+ID AY +GSVYF Sbjct: 218 FRKLAAYWEDDFNKDMANLNVLPPTAVTRVSEYVPEIVDFVQRIIDRGYAYPVTDGSVYF 277 Query: 283 DTDKFSN----YGKLQ-------KMQDSGEPSDDE---KRNKMDFALWKSHKPEEPYWDT 420 DT+ F Y KL+ ++ GE S K+ DFALWK+ KP EP WD+ Sbjct: 278 DTEAFEKGGHFYAKLEPWNKGNRELIAEGEGSLAALTGKKRPGDFALWKASKPGEPSWDS 337 Query: 421 PWGKGRPGWHIECSAMVSKVFGSQLDIHAGGVDLQFPHHENEEAQSCAYHNIRQWAN 591 PW KGRPGWHIECS M S + GS +DIH+GG+DL FPHH+NE AQS AY + QW N Sbjct: 338 PWSKGRPGWHIECSVMASALLGSNIDIHSGGIDLAFPHHDNELAQSEAYFDCPQWVN 394 >SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 32.7 bits (71), Expect = 0.042 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = -1 Query: 575 ILWYAHDCASSFS**GNCRSTPPAWMSNCDPNT 477 ++WY H C++ F GNC ++ +PNT Sbjct: 6 VIWYCHSCSNEFQRPGNCPRCNSDFVEMVEPNT 38 >SPCC330.05c |ura4||orotidine 5'-phosphate decarboxylase Ura4 |Schizosaccharomyces pombe|chr 3|||Manual Length = 264 Score = 29.9 bits (64), Expect = 0.29 Identities = 13/46 (28%), Positives = 26/46 (56%) Frame = +1 Query: 190 VSEHINSIEKFVQRLIDNDMAYVSKNGSVYFDTDKFSNYGKLQKMQ 327 + HI+ +E F Q +++ +A K+ + F+ KF++ G K+Q Sbjct: 61 IKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVKLQ 106 >SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosaccharomyces pombe|chr 2|||Manual Length = 376 Score = 29.1 bits (62), Expect = 0.51 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = +1 Query: 310 KLQKMQDSGEPSDDEKRNKMDFALWKSHKPEEPY 411 +LQKMQD+ + D+ +R K++ L K + EE + Sbjct: 296 RLQKMQDNAKELDESRRKKIEL-LEKKERDEEQF 328 >SPAC2G11.03c |vps45||vacuolar sorting protein Vps 45|Schizosaccharomyces pombe|chr 1|||Manual Length = 558 Score = 29.1 bits (62), Expect = 0.51 Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 4/65 (6%) Frame = -2 Query: 487 IQILC*PLQNILYASQVCLY----PRGYPNRVPRACDFSKEQSPFYFFFHHLMVHHCLAF 320 +++LC L++ YA + P+ + R+ + DF +S FF +L+V++ LA Sbjct: 77 LRLLCEELRDPKYAEYHLYFTNVIPKSFLERLAESDDFEAVKSIQEFFLDYLVVNNDLAS 136 Query: 319 FVICH 305 F I H Sbjct: 137 FNIPH 141 >SPBC405.03c |||membrane transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 341 Score = 26.6 bits (56), Expect = 2.7 Identities = 14/24 (58%), Positives = 15/24 (62%) Frame = -1 Query: 275 TEPFLLT*AMSLSISLCTNFSILL 204 T P L+T MSLSI L F ILL Sbjct: 287 TSPLLVTVGMSLSIPLALFFDILL 310 >SPBC660.14 |mik1||mitotic inhibitor kinase Mik1|Schizosaccharomyces pombe|chr 2|||Manual Length = 581 Score = 25.8 bits (54), Expect = 4.8 Identities = 10/30 (33%), Positives = 19/30 (63%) Frame = +2 Query: 317 KKCKTVVNHQMMKKEIKWTLLFGKVTSPRN 406 K+ T ++ ++ K KW+L G +T+PR+ Sbjct: 125 KRAVTSLSTGLLSKREKWSLWEGNLTNPRS 154 >SPBC29A3.17 |gef3||RhoGEF Gef3|Schizosaccharomyces pombe|chr 2|||Manual Length = 525 Score = 25.8 bits (54), Expect = 4.8 Identities = 19/76 (25%), Positives = 37/76 (48%), Gaps = 7/76 (9%) Frame = -3 Query: 240 INKPLYEFLNTINVFRYPRYDEWLCYIQ*CHIKPEFMLILFSNICE-VCFT------FEA 82 I+K + + N+ N+F P ++ L ++ +L L S IC+ + F+ F Sbjct: 296 IDKKIAKLQNSTNIFYVPEHEPILAFVHQLSSSYTNLLNLRSAICDWLKFSRYHYLKFFT 355 Query: 81 FLNDFVVYIRNTHSGD 34 F+ + V+ ++T S D Sbjct: 356 FVEAYSVFCKDTKSAD 371 >SPBC4B4.03 |rsc1||RSC complex subunit Rsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 25.4 bits (53), Expect = 6.3 Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 4/52 (7%) Frame = +1 Query: 163 VAKPLIITRVSEH-INSIEKFVQR---LIDNDMAYVSKNGSVYFDTDKFSNY 306 +A +I ++S+H +IE+FV + DN ++ + VY D D NY Sbjct: 257 MALEVIQKKLSKHRYETIEQFVDDFNLMFDNAKSFNDPSSQVYRDADFLKNY 308 >SPBC530.05 |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 743 Score = 25.4 bits (53), Expect = 6.3 Identities = 8/16 (50%), Positives = 12/16 (75%) Frame = -2 Query: 421 GYPNRVPRACDFSKEQ 374 G P R+PRACD +++ Sbjct: 2 GKPKRIPRACDMCRKR 17 >SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 25.4 bits (53), Expect = 6.3 Identities = 11/25 (44%), Positives = 15/25 (60%), Gaps = 1/25 (4%) Frame = -2 Query: 433 LYPRG-YPNRVPRACDFSKEQSPFY 362 L PRG YP+ ++CDF + FY Sbjct: 131 LSPRGNYPSEAEKSCDFYGQPLHFY 155 >SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 25.4 bits (53), Expect = 6.3 Identities = 12/40 (30%), Positives = 21/40 (52%) Frame = -1 Query: 338 SPLSCIFCNLP*FENLSVSKYTEPFLLT*AMSLSISLCTN 219 SPL + NL F+ +++S+Y P + + SL +N Sbjct: 71 SPLLAKWANLKAFQRVNISQYVHPLKEAEGIKIVASLSSN 110 >SPBC36.05c |clr6||histone deacetylase |Schizosaccharomyces pombe|chr 2|||Manual Length = 405 Score = 25.4 bits (53), Expect = 6.3 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = -3 Query: 255 ISHVVINKPLYEFLNTINVFRYPRYDEWLCY 163 + ++V+N LYE LN I R R D C+ Sbjct: 35 VHNLVVNYNLYEKLNVITPVRATRNDMTRCH 65 >SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 745 Score = 25.4 bits (53), Expect = 6.3 Identities = 13/57 (22%), Positives = 26/57 (45%), Gaps = 2/57 (3%) Frame = +1 Query: 235 IDNDMAYVSKNGSVYFDTDKFSNYGKLQK--MQDSGEPSDDEKRNKMDFALWKSHKP 399 + + ++V K S Y D ++ ++ D E S + +N +DF + +H P Sbjct: 577 VSDAKSFVKKIKSTYIDDSSSNSPSDIESNYSADDNEISKSKAQNAIDFNVTNTHSP 633 >SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3|||Manual Length = 378 Score = 25.4 bits (53), Expect = 6.3 Identities = 10/37 (27%), Positives = 20/37 (54%) Frame = +1 Query: 139 WLDMASLNVAKPLIITRVSEHINSIEKFVQRLIDNDM 249 W D+ VA+ + I EH+N+ +F + L + ++ Sbjct: 9 WTDVKDKPVARVVFIHGFGEHVNAYPEFFEALNERNI 45 >SPCC285.11 |ucp10||UBA/UAS domain protein Ucp10|Schizosaccharomyces pombe|chr 3|||Manual Length = 427 Score = 25.0 bits (52), Expect = 8.3 Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 6/60 (10%) Frame = +2 Query: 278 ILIPINSQTMANYKKCKTV------VNHQMMKKEIKWTLLFGKVTSPRNPIGIPPGVKAD 439 I P S+ + +KK TV V++ + +KE FG+ TS NP+ P K D Sbjct: 332 IRFPDGSRAVRRFKKDDTVESVYNYVDYMLFEKEEPEE--FGRATSSSNPVTPPSDYKHD 389 >SPAC9E9.12c |ybt1|abc1|ABC transporter Ybt1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1427 Score = 25.0 bits (52), Expect = 8.3 Identities = 10/33 (30%), Positives = 21/33 (63%), Gaps = 1/33 (3%) Frame = -1 Query: 110 FVKCVLLSRPFLMILSSISV-IPIAETIFILKN 15 F+ +L + ++ ++S+ +PIAE + +LKN Sbjct: 750 FLSTLLQHKTIILFTHNVSLCLPIAENVIVLKN 782 >SPCC1795.01c |mad3|SPCC895.02|mitotic spindle checkpoint protein Mad3|Schizosaccharomyces pombe|chr 3|||Manual Length = 310 Score = 25.0 bits (52), Expect = 8.3 Identities = 33/128 (25%), Positives = 47/128 (36%), Gaps = 8/128 (6%) Frame = +1 Query: 118 KQYEHEFWLDMA------SLNVAKPLIITRVSEHINSIEKFVQRLIDNDMAYVSKNGSVY 279 +Q+ H WL+ A + N PL T S +I I + ++ + SVY Sbjct: 192 QQFTHR-WLEFAPQSFSSNTNSVNPLQTTFESTNIQEISQSRTKISKPKFKF-----SVY 245 Query: 280 FDTDKFSNYGKLQKMQDSGEPSDDEKRNKMDFALWKSHK-P-EEPYWDTPWGKGRPGWHI 453 D D G+ Q G K N + W K P + P P GK + + Sbjct: 246 SDADGSGKDGQPGTWQTLGTVDQRRKENNISATSWVGEKLPLKSPRKLDPLGK----FQV 301 Query: 454 ECSAMVSK 477 C VSK Sbjct: 302 HCDEEVSK 309 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,669,594 Number of Sequences: 5004 Number of extensions: 59954 Number of successful extensions: 193 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 187 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 192 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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