Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0862
(593 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31170.1 68415.m03805 tRNA synthetase class I (C) family prot... 165 2e-41
At5g38830.1 68418.m04697 tRNA synthetase class I (C) family prot... 163 6e-41
At3g56300.1 68416.m06258 tRNA synthetase class I (C) family prot... 128 3e-30
At4g09570.1 68417.m01575 calcium-dependent protein kinase, putat... 31 0.77
At2g01270.1 68415.m00040 thioredoxin family protein low similari... 31 0.77
At2g44830.1 68415.m05582 protein kinase, putative similar to pro... 29 1.8
At2g04865.1 68415.m00502 expressed protein ; expression supporte... 29 1.8
At5g65930.2 68418.m08300 kinesin-like calmodulin-binding protein... 29 2.3
At5g65930.1 68418.m08299 kinesin-like calmodulin-binding protein... 29 2.3
At1g35670.1 68414.m04435 calcium-dependent protein kinase 2 (CDP... 29 2.3
At3g20010.1 68416.m02531 SNF2 domain-containing protein / helica... 28 5.4
At5g23450.2 68418.m02752 diacylglycerol kinase family protein co... 27 7.1
At5g23450.1 68418.m02751 diacylglycerol kinase family protein co... 27 7.1
At4g31010.2 68417.m04403 expressed protein 27 7.1
At4g31010.1 68417.m04404 expressed protein 27 7.1
At3g22730.1 68416.m02867 F-box family protein contains Pfam PF00... 27 7.1
At2g43000.1 68415.m05336 no apical meristem (NAM) family protein... 27 7.1
At5g04550.1 68418.m00455 expressed protein contains Pfam domain ... 27 9.4
At1g26610.1 68414.m03241 zinc finger (C2H2 type) family protein ... 27 9.4
>At2g31170.1 68415.m03805 tRNA synthetase class I (C) family protein
similar to cysteine-tRNA ligase [Escherichia coli]
GI:41203; contains Pfam profile PF01406: tRNA
synthetases class I (C)
Length = 563
Score = 165 bits (401), Expect = 2e-41
Identities = 80/168 (47%), Positives = 108/168 (64%), Gaps = 9/168 (5%)
Frame = +1
Query: 109 NIAKQYEHEFWLDMASLNVAKPLIITRVSEHINSIEKFVQRLIDNDMAYVSKNGSVYFDT 288
++++++ EF DM L P + RVS+HI I +++++DN AY +G +YF
Sbjct: 150 SLSRRFCEEFNRDMEQLQCLDPSVQPRVSDHIPQIIDLIKQILDNGYAY-KVDGDIYFSV 208
Query: 289 DKFSNYGKL--QKMQDS--GE--PSDDEKRNKMDFALWKSHKPEEPYWDTPWGKGRPGWH 450
DKF YGKL +K++D+ GE D K++ DFALWK+ K EP+W++PWG+GRPGWH
Sbjct: 209 DKFPTYGKLSGRKLEDNRAGERVAVDTRKKHPADFALWKAAKEGEPFWESPWGRGRPGWH 268
Query: 451 IECSAMVSKVFGSQLDIHAGGVDLQFPHHENEEAQSCA---YHNIRQW 585
IECSAM + G DIH GG+DL FPHHENE AQSCA NI W
Sbjct: 269 IECSAMSAAYLGYSFDIHGGGMDLVFPHHENEIAQSCAACDSSNISYW 316
>At5g38830.1 68418.m04697 tRNA synthetase class I (C) family protein
similar to SP|Q06752 Cysteinyl-tRNA synthetase (EC
6.1.1.16) (Cysteine--tRNA ligase) (CysRS) {Bacillus
subtilis}; contains Pfam profile PF01406: tRNA
synthetases class I (C)
Length = 511
Score = 163 bits (397), Expect = 6e-41
Identities = 77/158 (48%), Positives = 105/158 (66%), Gaps = 6/158 (3%)
Frame = +1
Query: 109 NIAKQYEHEFWLDMASLNVAKPLIITRVSEHINSIEKFVQRLIDNDMAYVSKNGSVYFDT 288
+++ ++ E+ +DM +L P RVSEH+++I K ++++I+ D YV + G V+F
Sbjct: 93 DLSNRFCDEYLVDMGALQCLPPTHQPRVSEHMDNIIKMIEKIIEKDCGYVVE-GDVFFSV 151
Query: 289 DKFSNYGKL--QKMQDS--GE--PSDDEKRNKMDFALWKSHKPEEPYWDTPWGKGRPGWH 450
DK NYGKL Q ++ + GE D KRN DFALWK+ KP+EP W++PWG GRPGWH
Sbjct: 152 DKSPNYGKLSGQLLEHTRAGERVAVDSRKRNPADFALWKAAKPDEPSWESPWGPGRPGWH 211
Query: 451 IECSAMVSKVFGSQLDIHAGGVDLQFPHHENEEAQSCA 564
IECSAM + DIH GG DL+FPHHENE AQ+CA
Sbjct: 212 IECSAMSVHYLSPKFDIHGGGADLKFPHHENEIAQTCA 249
>At3g56300.1 68416.m06258 tRNA synthetase class I (C) family protein
similar to cysteinyl-tRNA synthetase [Methanococcus
maripaludis] GI:6599476; contains Pfam profile PF01406:
tRNA synthetases class I (C)
Length = 489
Score = 128 bits (309), Expect = 3e-30
Identities = 68/158 (43%), Positives = 95/158 (60%), Gaps = 6/158 (3%)
Frame = +1
Query: 109 NIAKQYEHEFWLDMASLNVAKPLIITRVSEHINSIEKFVQRLIDNDMAYVSKNGSVYFDT 288
+++ ++ E+ LDMA+L P RVS+H+ I K ++++I+N Y + G V+F
Sbjct: 92 DLSNRFCEEYLLDMAALQCLLPTHQPRVSDHMEQIIKMIEKIIENGCGY-AVGGDVFFSV 150
Query: 289 DKFSNYGKL--QKMQ--DSGE--PSDDEKRNKMDFALWKSHKPEEPYWDTPWGKGRPGWH 450
DK +YG+L Q++ +G+ D KRN DFAL K+ K EP W++PWG GRPGWH
Sbjct: 151 DKSPSYGQLSGQRLDHTQAGKRVAVDSRKRNPADFALRKAAKSGEPSWESPWGHGRPGWH 210
Query: 451 IECSAMVSKVFGSQLDIHAGGVDLQFPHHENEEAQSCA 564
IE DIH GG DL+FPHHENE AQ+CA
Sbjct: 211 IE------------FDIHGGGADLKFPHHENEIAQTCA 236
>At4g09570.1 68417.m01575 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Arabidopsis thaliana] gi|604881|dbj|BAA04830;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 501
Score = 30.7 bits (66), Expect = 0.77
Identities = 19/61 (31%), Positives = 30/61 (49%)
Frame = +1
Query: 340 PSDDEKRNKMDFALWKSHKPEEPYWDTPWGKGRPGWHIECSAMVSKVFGSQLDIHAGGVD 519
PSDD K DF L +KP + +D G P + ++ K +G ++D+ + GV
Sbjct: 160 PSDDAKLKATDFGLSVFYKPGQYLYDV---VGSP--YYVAPEVLKKCYGPEIDVWSAGVI 214
Query: 520 L 522
L
Sbjct: 215 L 215
>At2g01270.1 68415.m00040 thioredoxin family protein low similarity
to quiescin [Homo sapiens] GI:13257405; contains Pfam
profiles PF00085: Thioredoxin, PF04777: Erv1 / Alr
family
Length = 495
Score = 30.7 bits (66), Expect = 0.77
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = +3
Query: 366 NGLCSLEKSQARGTLLGYPLG*RQTWLAYRMFCNGQQS 479
+G CS EKS TL +P+ + Y MFC G ++
Sbjct: 255 SGNCSYEKSGGNDTLGNFPICGKDVPRGYYMFCRGSKN 292
>At2g44830.1 68415.m05582 protein kinase, putative similar to
protein kinase PVPK-1 [Phaseolus vulgaris]
SWISS-PROT:P15792
Length = 765
Score = 29.5 bits (63), Expect = 1.8
Identities = 19/88 (21%), Positives = 42/88 (47%)
Frame = +1
Query: 154 SLNVAKPLIITRVSEHINSIEKFVQRLIDNDMAYVSKNGSVYFDTDKFSNYGKLQKMQDS 333
S+N+A+ + I S ++ ++ ++ D Y ++ S+ D+ + S + DS
Sbjct: 261 SMNLARAMSIANSSSATSTTQRS-DVVLSMDKNYFDRSISMVLDSFE-STKTSASRASDS 318
Query: 334 GEPSDDEKRNKMDFALWKSHKPEEPYWD 417
S++ + +L K HK +P+W+
Sbjct: 319 SGLSEESSWSNFTGSLNKPHKGNDPWWN 346
>At2g04865.1 68415.m00502 expressed protein ; expression supported
by MPSS
Length = 667
Score = 29.5 bits (63), Expect = 1.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 268 GSVYFDTDKFSNYGKLQKMQDSGEPSDDEKRNKMD 372
GS+ DTDKF KLQ+ D EP++ K D
Sbjct: 580 GSLLDDTDKFVEESKLQEEFDGEEPTESYDVKKED 614
>At5g65930.2 68418.m08300 kinesin-like calmodulin-binding protein
(ZWICHEL) identical to kinesin-like protein GI:2224925
from [Arabidopsis thaliana]
Length = 1260
Score = 29.1 bits (62), Expect = 2.3
Identities = 22/93 (23%), Positives = 41/93 (44%)
Frame = +1
Query: 109 NIAKQYEHEFWLDMASLNVAKPLIITRVSEHINSIEKFVQRLIDNDMAYVSKNGSVYFDT 288
NI K+ + E + K ++ RVSE N +E Q L + SKN +
Sbjct: 768 NIEKKKKEEVEIHQKRYEQEKKVLKLRVSELENKLEVLAQDLDSAESTIESKNSDMLLLQ 827
Query: 289 DKFSNYGKLQKMQDSGEPSDDEKRNKMDFALWK 387
+ +L++M++ D +++N+ A+ K
Sbjct: 828 NNLKELEELREMKE-----DIDRKNEQTAAILK 855
>At5g65930.1 68418.m08299 kinesin-like calmodulin-binding protein
(ZWICHEL) identical to kinesin-like protein GI:2224925
from [Arabidopsis thaliana]
Length = 1259
Score = 29.1 bits (62), Expect = 2.3
Identities = 22/93 (23%), Positives = 41/93 (44%)
Frame = +1
Query: 109 NIAKQYEHEFWLDMASLNVAKPLIITRVSEHINSIEKFVQRLIDNDMAYVSKNGSVYFDT 288
NI K+ + E + K ++ RVSE N +E Q L + SKN +
Sbjct: 767 NIEKKKKEEVEIHQKRYEQEKKVLKLRVSELENKLEVLAQDLDSAESTIESKNSDMLLLQ 826
Query: 289 DKFSNYGKLQKMQDSGEPSDDEKRNKMDFALWK 387
+ +L++M++ D +++N+ A+ K
Sbjct: 827 NNLKELEELREMKE-----DIDRKNEQTAAILK 854
>At1g35670.1 68414.m04435 calcium-dependent protein kinase 2 (CDPK2)
identical to calcium-dependent protein kinase
[Arabidopsis thaliana] gi|604881|dbj|BAA04830; contains
protein kinase domain, Pfam:PF00069; contains EF hand
domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 495
Score = 29.1 bits (62), Expect = 2.3
Identities = 18/61 (29%), Positives = 29/61 (47%)
Frame = +1
Query: 340 PSDDEKRNKMDFALWKSHKPEEPYWDTPWGKGRPGWHIECSAMVSKVFGSQLDIHAGGVD 519
P DD K DF L +KP + +D G P + ++ K +G ++D+ + GV
Sbjct: 161 PKDDAKLKATDFGLSVFYKPGQYLYDV---VGSP--YYVAPEVLKKCYGPEIDVWSAGVI 215
Query: 520 L 522
L
Sbjct: 216 L 216
>At3g20010.1 68416.m02531 SNF2 domain-containing protein / helicase
domain-containing protein / RING finger
domain-containing protein similar to transcription
factor RUSH-1alpha [Oryctolagus cuniculus] GI:1655930;
contains Pfam profiles PF00271: Helicase conserved
C-terminal domain, PF00176: SNF2 family N-terminal
domain, PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 1047
Score = 27.9 bits (59), Expect = 5.4
Identities = 19/65 (29%), Positives = 33/65 (50%)
Frame = +2
Query: 194 RNTLIVLRNSYRGLLITTWLM*VKTVQYILIPINSQTMANYKKCKTVVNHQMMKKEIKWT 373
+NT+ L + +R L + + I +PI+ + YKK + V+ M+++ K T
Sbjct: 581 QNTIDDLYSYFRFLRYDPYAVYKSFYSTIKVPISRNSCQGYKKLQAVLRAIMLRR-TKGT 639
Query: 374 LLFGK 388
LL GK
Sbjct: 640 LLDGK 644
>At5g23450.2 68418.m02752 diacylglycerol kinase family protein
contains INTERPRO domain, IPR001206, DAG-kinase
catalytic domain
Length = 763
Score = 27.5 bits (58), Expect = 7.1
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = -2
Query: 559 MIVPLHSHDEEIVDLLLQHGC 497
++ P HD+ +D+LL HGC
Sbjct: 667 VVAPNSEHDDGTMDMLLVHGC 687
>At5g23450.1 68418.m02751 diacylglycerol kinase family protein
contains INTERPRO domain, IPR001206, DAG-kinase
catalytic domain
Length = 763
Score = 27.5 bits (58), Expect = 7.1
Identities = 9/21 (42%), Positives = 14/21 (66%)
Frame = -2
Query: 559 MIVPLHSHDEEIVDLLLQHGC 497
++ P HD+ +D+LL HGC
Sbjct: 667 VVAPNSEHDDGTMDMLLVHGC 687
>At4g31010.2 68417.m04403 expressed protein
Length = 341
Score = 27.5 bits (58), Expect = 7.1
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +1
Query: 334 GEPSDDEKRNKMDFALWKSHKPEEP 408
G D +KR K+ LWK H+P P
Sbjct: 245 GRNYDPKKRPKIPLMLWKPHEPVYP 269
>At4g31010.1 68417.m04404 expressed protein
Length = 405
Score = 27.5 bits (58), Expect = 7.1
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +1
Query: 334 GEPSDDEKRNKMDFALWKSHKPEEP 408
G D +KR K+ LWK H+P P
Sbjct: 245 GRNYDPKKRPKIPLMLWKPHEPVYP 269
>At3g22730.1 68416.m02867 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box
protein interaction domain; similar to hypothetical
protein GB:AAD25583 from [Arabidopsis thaliana]
Length = 372
Score = 27.5 bits (58), Expect = 7.1
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = -3
Query: 270 TVFTYISHVVINKPLYEFLNTINVFRYPRYDEWLCYIQ*CH 148
+V I+++ ++ P EF +N+ YP Y E + + CH
Sbjct: 63 SVCPMIANLSVSAPSIEFKGALNLKNYPPYSEEVDIHEACH 103
>At2g43000.1 68415.m05336 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) domain;
Length = 275
Score = 27.5 bits (58), Expect = 7.1
Identities = 13/24 (54%), Positives = 16/24 (66%), Gaps = 2/24 (8%)
Frame = +2
Query: 368 WTL--LFGKVTSPRNPIGIPPGVK 433
WTL +F +VTS RNP +PP K
Sbjct: 159 WTLCRIFKRVTSQRNPTILPPNRK 182
>At5g04550.1 68418.m00455 expressed protein contains Pfam domain
PF05003: protein of unknown function (DUF668)
Length = 599
Score = 27.1 bits (57), Expect = 9.4
Identities = 12/48 (25%), Positives = 22/48 (45%)
Frame = +1
Query: 253 YVSKNGSVYFDTDKFSNYGKLQKMQDSGEPSDDEKRNKMDFALWKSHK 396
++S N S+Y +T+ ++ + K S E + D WK H+
Sbjct: 144 FISSNASLYQETEILADLEQTFKRMKSNESATDNLLEYQKKVTWKRHE 191
>At1g26610.1 68414.m03241 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 455
Score = 27.1 bits (57), Expect = 9.4
Identities = 20/91 (21%), Positives = 37/91 (40%), Gaps = 5/91 (5%)
Frame = +1
Query: 199 HINSIEKFVQR---LIDNDMAYVSKNGSVYFDTDKFSN--YGKLQKMQDSGEPSDDEKRN 363
++ ++E+ ++ ++DN M +NG V++D+D + + K DS D RN
Sbjct: 226 NVKNVEELCKKNKLVVDNQMRSGEENGDVHYDSDNSDSGYFRNGPKKLDSDVSVDGFFRN 285
Query: 364 KMDFALWKSHKPEEPYWDTPWGKGRPGWHIE 456
K D + R W+ E
Sbjct: 286 KTIMGSGSGFNSSPTKQDRDMNRFRKEWYKE 316
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,659,585
Number of Sequences: 28952
Number of extensions: 302587
Number of successful extensions: 866
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 841
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 859
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1180950720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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