Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0815
(519 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12770.1 68417.m02004 auxilin-related low similarity to SP|Q2... 31 0.61
At1g74120.1 68414.m08584 mitochondrial transcription termination... 29 1.9
At2g20840.1 68415.m02456 secretory carrier membrane protein (SCA... 29 2.5
At1g04120.1 68414.m00401 ABC transporter family protein Strong s... 28 3.3
At1g49760.1 68414.m05580 polyadenylate-binding protein, putative... 27 5.7
At1g29590.1 68414.m03618 eukaryotic translation initiation facto... 27 5.7
At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha catal... 27 7.6
At4g26990.1 68417.m03883 expressed protein 27 7.6
At4g12610.1 68417.m01987 transcription initiation factor IIF alp... 27 7.6
At2g12100.1 68415.m01300 Ulp1 protease family protein contains P... 27 7.6
At1g45090.1 68414.m05169 Ulp1 protease family protein similar to... 27 7.6
At1g01110.1 68414.m00014 expressed protein contains Prosite PS00... 27 7.6
>At4g12770.1 68417.m02004 auxilin-related low similarity to
SP|Q27974 Auxilin {Bos taurus}
Length = 909
Score = 30.7 bits (66), Expect = 0.61
Identities = 25/101 (24%), Positives = 41/101 (40%)
Frame = +1
Query: 13 AGIRHEAASGGRLTAKLHASSARQHHAGVYTCSEQPVQRVRVLVEPAPQTSSPDSSTIAP 192
A R AA+G R A+ A+ AR+ E V+ R VE A + ++ A
Sbjct: 595 AEARERAAAGAREKAEKAAAEARERANAEVREKEAKVRAERAAVERAAAEARGRAAAQAK 654
Query: 193 VTEPTQELVNYEALKYIVGRPFNLNCTLAVPLDSFEIVWKK 315
+ + + ++ V RP ++ P D F+ W K
Sbjct: 655 AKQQQENNNDLDSFFNSVSRPSSVPRQRTNPPDPFQDSWNK 695
>At1g74120.1 68414.m08584 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 445
Score = 29.1 bits (62), Expect = 1.9
Identities = 12/44 (27%), Positives = 25/44 (56%)
Frame = +1
Query: 289 DSFEIVWKKNSVPLGEVAGLKERYELQNKGLTFQIKGRSNEDDY 420
D+F++VWK+ V L E+ ++++ E + F I ++ +Y
Sbjct: 301 DAFKVVWKEPRVILYEIEDIEKKIEFLTNRMGFHINCLADVPEY 344
>At2g20840.1 68415.m02456 secretory carrier membrane protein (SCAMP)
family protein contains Pfam domain, PF04144: SCAMP
family
Length = 282
Score = 28.7 bits (61), Expect = 2.5
Identities = 21/74 (28%), Positives = 32/74 (43%), Gaps = 1/74 (1%)
Frame = +1
Query: 157 QTSSPDSSTIAPVTEPTQELVNYEALKYIVGRPFNLNCTLAVPLDSFEIVWKKNSVPLGE 336
Q+ S D I P PT L + P++ T+ +PLDS + + K +
Sbjct: 5 QSHSFDDGEINPFANPTSVPAATSKLSPLPPEPYDRGATMDIPLDSGKDLKAKEKELREK 64
Query: 337 VAGLKER-YELQNK 375
A LK R E++ K
Sbjct: 65 EAELKRREQEIKRK 78
>At1g04120.1 68414.m00401 ABC transporter family protein Strong
similarity to MRP-like ABC transporter gb|U92650 from A.
thaliana and canalicular multi-drug resistance protein
gb|L49379 from Rattus norvegicus
Length = 1514
Score = 28.3 bits (60), Expect = 3.3
Identities = 9/26 (34%), Positives = 19/26 (73%)
Frame = -1
Query: 291 IQWHGESAIQIERASNDVLQGLIIDQ 214
+ W G S IQ+ R+S+D+ + L++++
Sbjct: 195 LAWRGVSGIQVTRSSSDLQEPLLVEE 220
>At1g49760.1 68414.m05580 polyadenylate-binding protein, putative /
PABP, putative similar to poly(A)-binding protein
GB:AAF66825 GI:7673359 from [Nicotiana tabacum]
Length = 671
Score = 27.5 bits (58), Expect = 5.7
Identities = 12/39 (30%), Positives = 23/39 (58%)
Frame = +3
Query: 303 RMEEEQRASRRGGRPQGAVRAPEQGAHLPDQGPQ*RGRL 419
+ +++Q+ R GG +GA+ P+Q + + Q RGR+
Sbjct: 492 QQQQQQQQQRPGGGRRGALPQPQQPSPMMQQQMHPRGRM 530
>At1g29590.1 68414.m03618 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 285
Score = 27.5 bits (58), Expect = 5.7
Identities = 19/77 (24%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = -2
Query: 371 FWSSYRSLRPATS-PRGTLFFFHTISNESSGTARVQFKLNGRPTMYFKAS*LTSSWVGSV 195
FWS Y ++ P T G+ + E + G+ TM+F + L S+W+ ++
Sbjct: 148 FWSLYNNIHPPTKWVSGSDLYCFKDKIEPKWEDPICAN-GGKWTMFFPRATLESNWLNTL 206
Query: 194 TGAIVD--ESGDDV*GA 150
+ + + GD++ GA
Sbjct: 207 LALVGEQFDQGDEICGA 223
>At5g67100.1 68418.m08460 DNA-directed DNA polymerase alpha
catalytic subunit, putative similar to SP|O48653 DNA
polymerase alpha catalytic subunit (EC 2.7.7.7) {Oryza
sativa}; contains Pfam profiles: PF03175 DNA polymerase
type B, organellar and viral, PF00136 DNA polymerase
family B, PF03104 DNA polymerase family B, exonuclease
domain
Length = 1492
Score = 27.1 bits (57), Expect = 7.6
Identities = 14/47 (29%), Positives = 25/47 (53%)
Frame = +1
Query: 307 WKKNSVPLGEVAGLKERYELQNKGLTFQIKGRSNEDDYGNYTCGLKN 447
+++ VP GE LK Y +++ L +KG S G++T L++
Sbjct: 459 FERPDVPAGEQYVLKINYSFKDRPLPEDLKGESFSALLGSHTSALEH 505
>At4g26990.1 68417.m03883 expressed protein
Length = 474
Score = 27.1 bits (57), Expect = 7.6
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = +1
Query: 100 YTCSEQPVQRVRVLVEPAPQTSSPDSSTIAPVTEPTQELV 219
YT VQ +LV P+PQ S + V +Q+LV
Sbjct: 351 YTAQYHSVQAAPMLVNPSPQVMVARSGQLVYVQSVSQDLV 390
>At4g12610.1 68417.m01987 transcription initiation factor IIF alpha
subunit (TFIIF-alpha) family protein low similarity to
SP|Q05913 Transcription initiation factor IIF, alpha
subunit (TFIIF-alpha) (Transcription factor 5, large
chain) (TF5A) {Drosophila melanogaster}; contains Pfam
profile PF05793: Transcription initiation factor IIF,
alpha subunit (TFIIF-alpha)
Length = 543
Score = 27.1 bits (57), Expect = 7.6
Identities = 15/44 (34%), Positives = 22/44 (50%)
Frame = +1
Query: 70 SSARQHHAGVYTCSEQPVQRVRVLVEPAPQTSSPDSSTIAPVTE 201
SS ++ A + S P + V+ EPA +S S+ PVTE
Sbjct: 442 SSLKEERANTVSKSNTPTKAVKA--EPASAPASSSSAATGPVTE 483
>At2g12100.1 68415.m01300 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At5g28270, At2g05450,
At1g45090, At2g16180, At2g06750
Length = 1224
Score = 27.1 bits (57), Expect = 7.6
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +3
Query: 336 GGRPQGAVRAPEQGAHLPDQGPQ*RGRLRQLHVRPQEPNGTYQSLDGHGKRARQDDQ 506
GG P+ V APE Q P+ + R ++ N + +++ G G+ A ++D+
Sbjct: 733 GGNPEEEVVAPESQQLEEPQSPEVKVRQEFAFEVTEDGNPSAETICGEGEEALKEDK 789
>At1g45090.1 68414.m05169 Ulp1 protease family protein similar to
At5g28270, At2g12100, At2g05450, At2g16180, At2g06750;
contains Pfam profile PF02902: Ulp1 protease family,
C-terminal catalytic domain
Length = 1210
Score = 27.1 bits (57), Expect = 7.6
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +3
Query: 336 GGRPQGAVRAPEQGAHLPDQGPQ*RGRLRQLHVRPQEPNGTYQSLDGHGKRARQDDQ 506
GG P+ V APE Q P+ + R ++ N + +++ G G+ A ++D+
Sbjct: 724 GGNPEEEVVAPESQQLEEPQSPEVKVRQEFAFEVTEDGNPSAETICGEGEEALKEDK 780
>At1g01110.1 68414.m00014 expressed protein contains Prosite
PS00165: Serine/threonine dehydratases
pyridoxal-phosphate attachment site
Length = 364
Score = 27.1 bits (57), Expect = 7.6
Identities = 13/52 (25%), Positives = 24/52 (46%)
Frame = +2
Query: 71 LQLGSTTPESTHAASNPCSAYGSWWSLRLRHHHPTRQRLLRSPSPPKNWSIM 226
+++ ++ P S A +P R HH+ +R +PSP K+ I+
Sbjct: 155 VEIDTSQPYSRTGAGSPSRGQRPSSPSRTSHHYQSRNNFSATPSPAKSRPIL 206
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.314 0.130 0.386
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,652,551
Number of Sequences: 28952
Number of extensions: 235689
Number of successful extensions: 657
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 634
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 657
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 947539968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
- SilkBase 1999-2023 -