Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0800
(538 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 228 2e-60
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 227 3e-60
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 227 3e-60
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 227 3e-60
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 165 1e-41
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 165 1e-41
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 165 1e-41
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 165 1e-41
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 164 4e-41
At1g26170.1 68414.m03194 importin beta-2 subunit family protein ... 30 0.85
At1g43620.2 68414.m05008 UDP-glucose:sterol glucosyltransferase,... 29 1.5
At1g43620.1 68414.m05007 UDP-glucose:sterol glucosyltransferase,... 29 1.5
At1g22400.1 68414.m02801 UDP-glucoronosyl/UDP-glucosyl transfera... 29 1.5
At4g27010.1 68417.m03885 expressed protein ; expression support... 29 2.6
At3g17690.1 68416.m02258 cyclic nucleotide-binding transporter 2... 28 4.5
At1g73450.1 68414.m08503 protein kinase, putative similar to nuc... 28 4.5
At2g04620.1 68415.m00470 cation efflux family protein potential ... 27 6.0
At5g24080.1 68418.m02828 protein kinase family protein contains ... 27 7.9
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 228 bits (558), Expect = 2e-60
Identities = 110/138 (79%), Positives = 127/138 (92%)
Frame = +3
Query: 120 ETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKL 299
+ Q A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR+ESLTD SKL
Sbjct: 3 DVQMADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKL 62
Query: 300 DSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQAGADISM 479
D EL+I+++P+K+ TL+IID+GIGMTKADLVNNLGTIA+SGTK FMEALQAGAD+SM
Sbjct: 63 DGQPELFIRLVPDKSNKTLSIIDSGIGMTKADLVNNLGTIARSGTKEFMEALQAGADVSM 122
Query: 480 IGQFGVGFYSSYLVADRV 533
IGQFGVGFYS+YLVA++V
Sbjct: 123 IGQFGVGFYSAYLVAEKV 140
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 227 bits (556), Expect = 3e-60
Identities = 112/134 (83%), Positives = 122/134 (91%)
Frame = +3
Query: 132 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGK 311
A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR+ESLTD SKLD
Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQP 61
Query: 312 ELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQAGADISMIGQF 491
EL+I IIP+K TLTIID+GIGMTKADLVNNLGTIA+SGTK FMEAL AGAD+SMIGQF
Sbjct: 62 ELFIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEFMEALAAGADVSMIGQF 121
Query: 492 GVGFYSSYLVADRV 533
GVGFYS+YLVAD+V
Sbjct: 122 GVGFYSAYLVADKV 135
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 227 bits (556), Expect = 3e-60
Identities = 112/134 (83%), Positives = 122/134 (91%)
Frame = +3
Query: 132 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGK 311
A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR+ESLTD SKLD
Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQP 61
Query: 312 ELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQAGADISMIGQF 491
EL+I IIP+K TLTIID+GIGMTKADLVNNLGTIA+SGTK FMEAL AGAD+SMIGQF
Sbjct: 62 ELFIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEFMEALAAGADVSMIGQF 121
Query: 492 GVGFYSSYLVADRV 533
GVGFYS+YLVAD+V
Sbjct: 122 GVGFYSAYLVADKV 135
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 227 bits (556), Expect = 3e-60
Identities = 112/134 (83%), Positives = 122/134 (91%)
Frame = +3
Query: 132 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGK 311
A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR+ESLTD SKLD
Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFESLTDKSKLDGQP 61
Query: 312 ELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQAGADISMIGQF 491
EL+I IIP+K TLTIID+GIGMTKADLVNNLGTIA+SGTK FMEAL AGAD+SMIGQF
Sbjct: 62 ELFIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEFMEALAAGADVSMIGQF 121
Query: 492 GVGFYSSYLVADRV 533
GVGFYS+YLVAD+V
Sbjct: 122 GVGFYSAYLVADKV 135
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 165 bits (402), Expect = 1e-41
Identities = 83/140 (59%), Positives = 108/140 (77%), Gaps = 2/140 (1%)
Frame = +3
Query: 120 ETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKL 299
+T + E F FQAE+++LM +IIN+ YSNK+IFLRELISN+SDALDKIR+ +LTD L
Sbjct: 70 KTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIFLRELISNASDALDKIRFLALTDKDVL 129
Query: 300 DSGK--ELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQAGADI 473
G +L I+I +K + L+I D GIGMTK DL+ NLGTIAKSGT AF+E +Q+ D+
Sbjct: 130 GEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKEDLIKNLGTIAKSGTSAFVEKMQSSGDL 189
Query: 474 SMIGQFGVGFYSSYLVADRV 533
++IGQFGVGFYS+YLVAD +
Sbjct: 190 NLIGQFGVGFYSAYLVADYI 209
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 165 bits (402), Expect = 1e-41
Identities = 83/140 (59%), Positives = 108/140 (77%), Gaps = 2/140 (1%)
Frame = +3
Query: 120 ETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKL 299
+T + E F FQAE+++LM +IIN+ YSNK+IFLRELISN+SDALDKIR+ +LTD L
Sbjct: 70 KTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIFLRELISNASDALDKIRFLALTDKDVL 129
Query: 300 DSGK--ELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQAGADI 473
G +L I+I +K + L+I D GIGMTK DL+ NLGTIAKSGT AF+E +Q+ D+
Sbjct: 130 GEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKEDLIKNLGTIAKSGTSAFVEKMQSSGDL 189
Query: 474 SMIGQFGVGFYSSYLVADRV 533
++IGQFGVGFYS+YLVAD +
Sbjct: 190 NLIGQFGVGFYSAYLVADYI 209
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 165 bits (402), Expect = 1e-41
Identities = 79/145 (54%), Positives = 114/145 (78%), Gaps = 4/145 (2%)
Frame = +3
Query: 111 EEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDP 290
E+ T+ E F +QAE+++L+ LI+++ YS+KE+FLREL+SN+SDALDK+R+ S+T+P
Sbjct: 67 EKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSHKEVFLRELVSNASDALDKLRFLSVTEP 126
Query: 291 SKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQ---- 458
S L G +L I+I P+ + GT+TI DTGIGMTK +L++ LGTIA+SGT F++AL+
Sbjct: 127 SLLGDGGDLEIRIKPDPDNGTITITDTGIGMTKEELIDCLGTIAQSGTSKFLKALKENKD 186
Query: 459 AGADISMIGQFGVGFYSSYLVADRV 533
GAD +IGQFGVGFYS++LVA++V
Sbjct: 187 LGADNGLIGQFGVGFYSAFLVAEKV 211
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 165 bits (402), Expect = 1e-41
Identities = 79/145 (54%), Positives = 114/145 (78%), Gaps = 4/145 (2%)
Frame = +3
Query: 111 EEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDP 290
E+ T+ E F +QAE+++L+ LI+++ YS+KE+FLREL+SN+SDALDK+R+ S+T+P
Sbjct: 67 EKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSHKEVFLRELVSNASDALDKLRFLSVTEP 126
Query: 291 SKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQ---- 458
S L G +L I+I P+ + GT+TI DTGIGMTK +L++ LGTIA+SGT F++AL+
Sbjct: 127 SLLGDGGDLEIRIKPDPDNGTITITDTGIGMTKEELIDCLGTIAQSGTSKFLKALKENKD 186
Query: 459 AGADISMIGQFGVGFYSSYLVADRV 533
GAD +IGQFGVGFYS++LVA++V
Sbjct: 187 LGADNGLIGQFGVGFYSAFLVAEKV 211
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 164 bits (398), Expect = 4e-41
Identities = 80/139 (57%), Positives = 107/139 (76%), Gaps = 4/139 (2%)
Frame = +3
Query: 129 PAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSG 308
P E F +QAE+++LM LI+N+ YSNKE+FLRELISN+SDALDK+RY S+T+P
Sbjct: 91 PPPAEKFEYQAEVSRLMDLIVNSLYSNKEVFLRELISNASDALDKLRYLSVTNPELSKDA 150
Query: 309 KELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQ----AGADIS 476
+L I+I +K G +T+ D+GIGMT+ +LV+ LGTIA+SGT FM+AL+ AG D +
Sbjct: 151 PDLDIRIYADKENGIITLTDSGIGMTRQELVDCLGTIAQSGTAKFMKALKDSKDAGGDNN 210
Query: 477 MIGQFGVGFYSSYLVADRV 533
+IGQFGVGFYS++LVADRV
Sbjct: 211 LIGQFGVGFYSAFLVADRV 229
>At1g26170.1 68414.m03194 importin beta-2 subunit family protein
similar to Importin9 isoform 1 [Mus musculus]
GI:15186756; contains Pfam profile PF03810:
Importin-beta N-terminal domain
Length = 931
Score = 30.3 bits (65), Expect = 0.85
Identities = 15/50 (30%), Positives = 30/50 (60%)
Frame = +3
Query: 156 QAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDS 305
++E+ + S + +TF S+ +++LR I + D+ D RY+S + LD+
Sbjct: 259 ESELMGMFSPLWHTFESSLQVYLRSSIDGAEDSYDG-RYDSDGEEKSLDT 307
>At1g43620.2 68414.m05008 UDP-glucose:sterol glucosyltransferase,
putative similar to UDP-glucose:sterol
glucosyltransferase [Arabidopsis thaliana] GI:2462931;
contains Pfam profile: PF03033 glycosyltransferase
family 28 N-terminal domain
Length = 615
Score = 29.5 bits (63), Expect = 1.5
Identities = 13/52 (25%), Positives = 30/52 (57%)
Frame = +3
Query: 219 FLRELISNSSDALDKIRYESLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTG 374
+ R L S A+ ++ + L++ K ++L ++++ +N+GT+ +ID G
Sbjct: 75 YSRSLTEKGSPAIHNLKLDRLSEQEK----QKLIVELVRIQNDGTVEVIDNG 122
>At1g43620.1 68414.m05007 UDP-glucose:sterol glucosyltransferase,
putative similar to UDP-glucose:sterol
glucosyltransferase [Arabidopsis thaliana] GI:2462931;
contains Pfam profile: PF03033 glycosyltransferase
family 28 N-terminal domain
Length = 615
Score = 29.5 bits (63), Expect = 1.5
Identities = 13/52 (25%), Positives = 30/52 (57%)
Frame = +3
Query: 219 FLRELISNSSDALDKIRYESLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTG 374
+ R L S A+ ++ + L++ K ++L ++++ +N+GT+ +ID G
Sbjct: 75 YSRSLTEKGSPAIHNLKLDRLSEQEK----QKLIVELVRIQNDGTVEVIDNG 122
>At1g22400.1 68414.m02801 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 489
Score = 29.5 bits (63), Expect = 1.5
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +3
Query: 189 INTFYSNKEIFLRELISNSSDALDKIRYESLTD 287
+NT Y++ FLR SN+ D L R+ES+ D
Sbjct: 45 VNTVYNHNR-FLRSRGSNALDGLPSFRFESIAD 76
>At4g27010.1 68417.m03885 expressed protein ; expression supported
by MPSS
Length = 2535
Score = 28.7 bits (61), Expect = 2.6
Identities = 17/50 (34%), Positives = 30/50 (60%)
Frame = +3
Query: 213 EIFLRELISNSSDALDKIRYESLTDPSKLDSGKELYIKIIPNKNEGTLTI 362
+I++ EL SN D++++ E LDS K +Y++ +PN+ EG+ I
Sbjct: 580 DIWISELCSNPIDSVEEA--EMCFHIKLLDSLK-IYVRAVPNELEGSFDI 626
>At3g17690.1 68416.m02258 cyclic nucleotide-binding transporter 2 /
CNBT2 (CNGC19) identical to cyclic nucleotide-binding
transporter 2 (CNBT2) GI:8131900 from [Arabidopsis
thaliana]; member of the cyclic nucleotide-gated channel
family (CNGC)- see PMID:11500563
Length = 743
Score = 27.9 bits (59), Expect = 4.5
Identities = 20/72 (27%), Positives = 36/72 (50%)
Frame = +3
Query: 99 KKMPEEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYES 278
+++PE++ + EVE + + A L+ F + + R++ + L K+R S
Sbjct: 506 RRLPEDIRKRVREVERYTWAATRGVNEELL---FENMPDDLQRDIRRHLFKFLKKVRIFS 562
Query: 279 LTDPSKLDSGKE 314
L D S LDS +E
Sbjct: 563 LMDESVLDSIRE 574
>At1g73450.1 68414.m08503 protein kinase, putative similar to
nuclear serine/threonine protein kinase GI:3582644 from
[Rattus norvegicus]
Length = 1152
Score = 27.9 bits (59), Expect = 4.5
Identities = 21/62 (33%), Positives = 32/62 (51%), Gaps = 6/62 (9%)
Frame = +3
Query: 150 AFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDA---LDKIRYE---SLTDPSKLDSGK 311
A Q+ + ++ + N +SN E LR +SN SD L K+ E S D ++ +GK
Sbjct: 2 ADQSSVDGILEFLRNNRFSNAEEALRNELSNRSDINGFLQKLMLEEKDSSKDSNERANGK 61
Query: 312 EL 317
EL
Sbjct: 62 EL 63
>At2g04620.1 68415.m00470 cation efflux family protein potential
member of the cation diffusion facilitator (CDF) family,
or cation efflux (CE) family, see PMID:11500563
Length = 798
Score = 27.5 bits (58), Expect = 6.0
Identities = 12/56 (21%), Positives = 21/56 (37%), Gaps = 2/56 (3%)
Frame = +2
Query: 320 HQDHSQQERGHSYD--HRYRYWYDQGRFGEQFGNHREIWY*SFHGGSSSRCRHQHD 481
H++H Q H ++ H + + + + NH H + C H HD
Sbjct: 579 HEEHHQHSDSHKHEEHHEHDHHHHSHSHKHEECNHNHDHEHQSHSHNHEECNHNHD 634
>At5g24080.1 68418.m02828 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 470
Score = 27.1 bits (57), Expect = 7.9
Identities = 15/28 (53%), Positives = 19/28 (67%)
Frame = -1
Query: 397 KSALVIPIPVSMIVRVPSFLLGMILMYS 314
+ LVIPI V M+V V LLGM+L Y+
Sbjct: 69 QKVLVIPIVVGMLVLVA--LLGMLLYYN 94
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,516,988
Number of Sequences: 28952
Number of extensions: 243597
Number of successful extensions: 681
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 660
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 674
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 993966856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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