BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0719 (554 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 127 1e-30 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 54 1e-08 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 47 2e-06 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 47 2e-06 SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 46 3e-06 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 44 2e-05 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 41 1e-04 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 40 2e-04 SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 39 4e-04 SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 36 0.004 SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 36 0.004 SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 34 0.012 SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 33 0.037 SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 31 0.11 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 28 1.1 SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces p... 28 1.1 SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 1.9 SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 26 3.2 SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 5.7 SPAC12B10.04 |||tubulin-tyrosine ligase |Schizosaccharomyces pom... 25 7.5 SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 25 7.5 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 25 7.5 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 25 9.9 >SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizosaccharomyces pombe|chr 3|||Manual Length = 139 Score = 127 bits (306), Expect = 1e-30 Identities = 52/113 (46%), Positives = 83/113 (73%) Frame = +1 Query: 214 NFRLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGT 393 NF+LLEELE+G+KG+G+ + S+GL + DD+TL+ W I+GP + +ENR+YSLKI C Sbjct: 7 NFKLLEELEKGEKGLGESSCSYGLTNADDITLSDWNATILGPAHSVHENRIYSLKIHCDA 66 Query: 394 RYPDEPPTARFISRIHMNCVNSQTGLVDNRQVPILARWQRDYTIKTVLQEVRR 552 YPD PP F+SRI++ V+ +TG V+ ++ L W+R+Y+++TVL ++++ Sbjct: 67 NYPDAPPIVTFVSRINLPGVDGETGKVNPHKIDCLRHWKREYSMETVLLDLKK 119 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 54.4 bits (125), Expect = 1e-08 Identities = 36/107 (33%), Positives = 53/107 (49%), Gaps = 1/107 (0%) Frame = +1 Query: 220 RLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRY 399 R+ +EL Q+ G W + D ++H GM GP TPYE + + IE Y Sbjct: 8 RIAKELADVQQDKQAGIQVWTINDD----ISHLKGMFRGPEGTPYEGGYFVVDIEIPIDY 63 Query: 400 PDEPPTARFISRIHMNCVNSQTGLVDNRQVPILA-RWQRDYTIKTVL 537 P PP F ++I+ V+SQTG + + IL +W YT+K+ L Sbjct: 64 PFRPPKMNFDTKIYHPNVSSQTGAI---CLDILKDQWSPVYTMKSAL 107 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 47.2 bits (107), Expect = 2e-06 Identities = 31/111 (27%), Positives = 55/111 (49%), Gaps = 1/111 (0%) Frame = +1 Query: 220 RLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRY 399 RL+ + ++ Q+ G +S SD+ M W +IIGP TP+E+ + L + +Y Sbjct: 8 RLMRDFKRMQQDPPAG-VSASPVSDNVML---WNAVIIGPADTPFEDGTFKLVLSFDEQY 63 Query: 400 PDEPPTARFISRI-HMNCVNSQTGLVDNRQVPILARWQRDYTIKTVLQEVR 549 P++PP +F+S + H N + +D Q RW Y + +L ++ Sbjct: 64 PNKPPLVKFVSTMFHPNVYANGELCLDILQ----NRWSPTYDVAAILTSIQ 110 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 47.2 bits (107), Expect = 2e-06 Identities = 27/83 (32%), Positives = 37/83 (44%), Gaps = 1/83 (1%) Frame = +1 Query: 292 DDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMNCVNSQTG 468 D D L HW G I GP T YE + + + YP PPT F S + H N S Sbjct: 53 DSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNI 112 Query: 469 LVDNRQVPILARWQRDYTIKTVL 537 +D + +W Y ++T+L Sbjct: 113 CLD----ILKDKWSAVYNVQTIL 131 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 46.4 bits (105), Expect = 3e-06 Identities = 27/81 (33%), Positives = 39/81 (48%), Gaps = 1/81 (1%) Frame = +1 Query: 307 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLVDNRQ 486 L HW I+GP +PY ++ L I T YP +PP F +RI+ +NS + Sbjct: 30 LFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSIC---- 85 Query: 487 VPILA-RWQRDYTIKTVLQEV 546 + IL +W TI VL + Sbjct: 86 LDILRDQWSPALTISKVLLSI 106 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 43.6 bits (98), Expect = 2e-05 Identities = 27/77 (35%), Positives = 37/77 (48%), Gaps = 1/77 (1%) Frame = +1 Query: 220 RLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRY 399 RL +E Q G G + L+ D+ + HW +I GP TPYE + L I Y Sbjct: 7 RLHKEYASFQAGDISGLLL--LKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGY 64 Query: 400 PDEPPTARFISRI-HMN 447 P PP+ F ++I H N Sbjct: 65 PISPPSVYFQTKIVHPN 81 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 40.7 bits (91), Expect = 1e-04 Identities = 23/69 (33%), Positives = 38/69 (55%) Frame = +1 Query: 220 RLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRY 399 RL++E ++ + DG I+ G ++DD W +I GP TP+E +Y ++ + Y Sbjct: 9 RLMKEYKELTENGPDG-ITAGPSNEDDFFT--WDCLIQGPDGTPFEGGLYPATLKFPSDY 65 Query: 400 PDEPPTARF 426 P PPT +F Sbjct: 66 PLGPPTLKF 74 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 39.9 bits (89), Expect = 2e-04 Identities = 27/84 (32%), Positives = 39/84 (46%), Gaps = 2/84 (2%) Frame = +1 Query: 220 RLLEELEQGQKGVGDGTISWGLESDDD-MTLTHWTGMIIGPPRTPYENRMYSLKIECGTR 396 RL EE +Q ++ G + +S D + L +W I G P+T +E +Y L + Sbjct: 8 RLQEERKQWRRDHPFGFYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEE 67 Query: 397 YPDEPPTARFISRI-HMNCVNSQT 465 YP PP RF + H N S T Sbjct: 68 YPTRPPKCRFTPPLFHPNVYPSGT 91 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 39.1 bits (87), Expect = 4e-04 Identities = 27/76 (35%), Positives = 37/76 (48%), Gaps = 1/76 (1%) Frame = +1 Query: 223 LLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYP 402 L ++L++ QK G S GL DD ++ W MIIGP T YE + + YP Sbjct: 10 LRKQLKEIQKNPPQG-FSVGLV--DDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYP 66 Query: 403 DEPPTARFISRI-HMN 447 PP +F + I H N Sbjct: 67 LMPPKMKFTTEIWHPN 82 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 35.9 bits (79), Expect = 0.004 Identities = 16/42 (38%), Positives = 21/42 (50%) Frame = +1 Query: 307 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFIS 432 + W +I GPP TPYE Y + YP +PP R I+ Sbjct: 34 ILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMIT 75 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 35.9 bits (79), Expect = 0.004 Identities = 15/48 (31%), Positives = 23/48 (47%) Frame = +1 Query: 298 DMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 441 D L HW +GP + Y + ++ YP +PPT F +RI+ Sbjct: 33 DDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIY 80 >SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 34.3 bits (75), Expect = 0.012 Identities = 14/47 (29%), Positives = 26/47 (55%) Frame = +1 Query: 334 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLV 474 GP TPY ++ + +E + YP + P+ F++RI ++ +G V Sbjct: 38 GPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSV 84 >SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Schizosaccharomyces pombe|chr 1|||Manual Length = 148 Score = 32.7 bits (71), Expect = 0.037 Identities = 18/74 (24%), Positives = 38/74 (51%) Frame = +1 Query: 220 RLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRY 399 R+++E+E + G ++ E + L ++ + GP ++ YE + L++ Y Sbjct: 6 RIIKEIETLTRDPPPGIVAAPTEDN----LRYFKITMEGPQQSAYEGGKFHLELFLPDEY 61 Query: 400 PDEPPTARFISRIH 441 P PP RF+++I+ Sbjct: 62 PMMPPNVRFLTKIY 75 >SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1075 Score = 31.1 bits (67), Expect = 0.11 Identities = 20/64 (31%), Positives = 32/64 (50%), Gaps = 4/64 (6%) Frame = +1 Query: 205 CHGNFRLLEELEQGQKGVGDGTISWGLESDDDMTLTHW----TGMIIGPPRTPYENRMYS 372 C ++RLL E+ G +WG+ +DD ++ W +G I+ +TPYE M Sbjct: 337 CGPSYRLLPVEERNISCSGRDDFAWGILNDDWVSHPTWASEESGFIV-QRKTPYEEAMTK 395 Query: 373 LKIE 384 L+ E Sbjct: 396 LEEE 399 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 27.9 bits (59), Expect = 1.1 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = -3 Query: 504 SSKNWDLSIIHKSSLTIDTVHMNPRNKAGCWW 409 S+ NW S+++ SL D + NP+NK W Sbjct: 291 STVNWVQSVLNLPSLLSDDLMANPKNKERFEW 322 >SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 584 Score = 27.9 bits (59), Expect = 1.1 Identities = 12/22 (54%), Positives = 16/22 (72%) Frame = -3 Query: 324 SSPMSKSHIIIAFKTPRNGSIA 259 SSPMS+SH+ I+ + PR G A Sbjct: 439 SSPMSESHVQISSEMPRTGMSA 460 >SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 834 Score = 27.1 bits (57), Expect = 1.9 Identities = 13/32 (40%), Positives = 20/32 (62%) Frame = -3 Query: 144 KLRLIQSSFIA*ALPKQIRFI*SKYGHLQQSN 49 K RL++S F +LP Q+RF+ Y +L+ N Sbjct: 27 KFRLLESQFALYSLPCQLRFV--HYNNLEDPN 56 >SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 26.2 bits (55), Expect = 3.2 Identities = 9/37 (24%), Positives = 19/37 (51%) Frame = +1 Query: 331 IGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 441 I P Y+ + +I+ YP +PP + +++I+ Sbjct: 58 IRPDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIY 94 >SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 25.4 bits (53), Expect = 5.7 Identities = 17/51 (33%), Positives = 26/51 (50%) Frame = -3 Query: 348 CPWWANNHSSPMSKSHIIIAFKTPRNGSIAHSLLALFKFF*ETKIPVAPQR 196 C W +N S + S + + +++ S A A+F+ ETKIPV P R Sbjct: 24 CECWDHNLCSSQA-SEMDLIYQSQDTHSCASKQDAVFQLLSETKIPV-PNR 72 >SPAC12B10.04 |||tubulin-tyrosine ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 403 Score = 25.0 bits (52), Expect = 7.5 Identities = 13/47 (27%), Positives = 21/47 (44%) Frame = -3 Query: 540 LKNCFDGIVTLPSSKNWDLSIIHKSSLTIDTVHMNPRNKAGCWWLIR 400 LKN + S+K + + D V+ NP+ K C ++IR Sbjct: 27 LKNSGKELTFTNSAKEANFQWAQYEDIDFDEVYKNPKTKLCCSYVIR 73 >SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1841 Score = 25.0 bits (52), Expect = 7.5 Identities = 16/49 (32%), Positives = 24/49 (48%) Frame = -2 Query: 334 Q*SFQSNE*ESYHHRFQDPKKWFHRPLPFGLVQVLLRDENSRGTTTPDD 188 Q S QS++ + Q P K FHRP P L + + R + T+ D+ Sbjct: 785 QMSRQSSQPSTVSTILQIPDKKFHRPFPRHLHRYVGRSASESLTSEKDE 833 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 25.0 bits (52), Expect = 7.5 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = -1 Query: 287 SRPQEMVPSPTPFWPCSSSSKRRKFPWHHN 198 S P MV SPT + CS++ + FP H+ Sbjct: 111 SHPSYMVQSPTSYHACSNN--QSPFPHSHH 138 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 24.6 bits (51), Expect = 9.9 Identities = 10/20 (50%), Positives = 14/20 (70%) Frame = -2 Query: 274 KWFHRPLPFGLVQVLLRDEN 215 K F RP+P L+Q+ L D+N Sbjct: 890 KVFQRPIPLLLLQLFLVDDN 909 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,374,549 Number of Sequences: 5004 Number of extensions: 49628 Number of successful extensions: 139 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 139 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 231978230 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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