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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= P5PG0719
         (554 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos...   127   1e-30
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch...    54   1e-08
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    47   2e-06
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2...    47   2e-06
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha...    46   3e-06
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac...    44   2e-05
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo...    41   1e-04
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...    40   2e-04
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac...    39   4e-04
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    36   0.004
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac...    36   0.004
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch...    34   0.012
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch...    33   0.037
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst...    31   0.11 
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki...    28   1.1  
SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces p...    28   1.1  
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo...    27   1.9  
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac...    26   3.2  
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    25   5.7  
SPAC12B10.04 |||tubulin-tyrosine ligase |Schizosaccharomyces pom...    25   7.5  
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1...    25   7.5  
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    25   7.5  
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb...    25   9.9  

>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
           Mms2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 139

 Score =  127 bits (306), Expect = 1e-30
 Identities = 52/113 (46%), Positives = 83/113 (73%)
 Frame = +1

Query: 214 NFRLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGT 393
           NF+LLEELE+G+KG+G+ + S+GL + DD+TL+ W   I+GP  + +ENR+YSLKI C  
Sbjct: 7   NFKLLEELEKGEKGLGESSCSYGLTNADDITLSDWNATILGPAHSVHENRIYSLKIHCDA 66

Query: 394 RYPDEPPTARFISRIHMNCVNSQTGLVDNRQVPILARWQRDYTIKTVLQEVRR 552
            YPD PP   F+SRI++  V+ +TG V+  ++  L  W+R+Y+++TVL ++++
Sbjct: 67  NYPDAPPIVTFVSRINLPGVDGETGKVNPHKIDCLRHWKREYSMETVLLDLKK 119


>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
           Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 217

 Score = 54.4 bits (125), Expect = 1e-08
 Identities = 36/107 (33%), Positives = 53/107 (49%), Gaps = 1/107 (0%)
 Frame = +1

Query: 220 RLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRY 399
           R+ +EL   Q+    G   W +  D    ++H  GM  GP  TPYE   + + IE    Y
Sbjct: 8   RIAKELADVQQDKQAGIQVWTINDD----ISHLKGMFRGPEGTPYEGGYFVVDIEIPIDY 63

Query: 400 PDEPPTARFISRIHMNCVNSQTGLVDNRQVPILA-RWQRDYTIKTVL 537
           P  PP   F ++I+   V+SQTG +    + IL  +W   YT+K+ L
Sbjct: 64  PFRPPKMNFDTKIYHPNVSSQTGAI---CLDILKDQWSPVYTMKSAL 107


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 47.2 bits (107), Expect = 2e-06
 Identities = 31/111 (27%), Positives = 55/111 (49%), Gaps = 1/111 (0%)
 Frame = +1

Query: 220 RLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRY 399
           RL+ + ++ Q+    G +S    SD+ M    W  +IIGP  TP+E+  + L +    +Y
Sbjct: 8   RLMRDFKRMQQDPPAG-VSASPVSDNVML---WNAVIIGPADTPFEDGTFKLVLSFDEQY 63

Query: 400 PDEPPTARFISRI-HMNCVNSQTGLVDNRQVPILARWQRDYTIKTVLQEVR 549
           P++PP  +F+S + H N   +    +D  Q     RW   Y +  +L  ++
Sbjct: 64  PNKPPLVKFVSTMFHPNVYANGELCLDILQ----NRWSPTYDVAAILTSIQ 110


>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 176

 Score = 47.2 bits (107), Expect = 2e-06
 Identities = 27/83 (32%), Positives = 37/83 (44%), Gaps = 1/83 (1%)
 Frame = +1

Query: 292 DDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMNCVNSQTG 468
           D D  L HW G I GP  T YE   + + +     YP  PPT  F S + H N   S   
Sbjct: 53  DSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNI 112

Query: 469 LVDNRQVPILARWQRDYTIKTVL 537
            +D     +  +W   Y ++T+L
Sbjct: 113 CLD----ILKDKWSAVYNVQTIL 131


>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
           Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score = 46.4 bits (105), Expect = 3e-06
 Identities = 27/81 (33%), Positives = 39/81 (48%), Gaps = 1/81 (1%)
 Frame = +1

Query: 307 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLVDNRQ 486
           L HW   I+GP  +PY   ++ L I   T YP +PP   F +RI+   +NS   +     
Sbjct: 30  LFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSIC---- 85

Query: 487 VPILA-RWQRDYTIKTVLQEV 546
           + IL  +W    TI  VL  +
Sbjct: 86  LDILRDQWSPALTISKVLLSI 106


>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
           Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 160

 Score = 43.6 bits (98), Expect = 2e-05
 Identities = 27/77 (35%), Positives = 37/77 (48%), Gaps = 1/77 (1%)
 Frame = +1

Query: 220 RLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRY 399
           RL +E    Q G   G +   L+   D+ + HW  +I GP  TPYE   + L I     Y
Sbjct: 7   RLHKEYASFQAGDISGLLL--LKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGY 64

Query: 400 PDEPPTARFISRI-HMN 447
           P  PP+  F ++I H N
Sbjct: 65  PISPPSVYFQTKIVHPN 81


>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
           Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 40.7 bits (91), Expect = 1e-04
 Identities = 23/69 (33%), Positives = 38/69 (55%)
 Frame = +1

Query: 220 RLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRY 399
           RL++E ++  +   DG I+ G  ++DD     W  +I GP  TP+E  +Y   ++  + Y
Sbjct: 9   RLMKEYKELTENGPDG-ITAGPSNEDDFFT--WDCLIQGPDGTPFEGGLYPATLKFPSDY 65

Query: 400 PDEPPTARF 426
           P  PPT +F
Sbjct: 66  PLGPPTLKF 74


>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score = 39.9 bits (89), Expect = 2e-04
 Identities = 27/84 (32%), Positives = 39/84 (46%), Gaps = 2/84 (2%)
 Frame = +1

Query: 220 RLLEELEQGQKGVGDGTISWGLESDDD-MTLTHWTGMIIGPPRTPYENRMYSLKIECGTR 396
           RL EE +Q ++    G  +   +S D  + L +W   I G P+T +E  +Y L +     
Sbjct: 8   RLQEERKQWRRDHPFGFYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEE 67

Query: 397 YPDEPPTARFISRI-HMNCVNSQT 465
           YP  PP  RF   + H N   S T
Sbjct: 68  YPTRPPKCRFTPPLFHPNVYPSGT 91


>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
           Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 167

 Score = 39.1 bits (87), Expect = 4e-04
 Identities = 27/76 (35%), Positives = 37/76 (48%), Gaps = 1/76 (1%)
 Frame = +1

Query: 223 LLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYP 402
           L ++L++ QK    G  S GL   DD ++  W  MIIGP  T YE   +   +     YP
Sbjct: 10  LRKQLKEIQKNPPQG-FSVGLV--DDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYP 66

Query: 403 DEPPTARFISRI-HMN 447
             PP  +F + I H N
Sbjct: 67  LMPPKMKFTTEIWHPN 82


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 35.9 bits (79), Expect = 0.004
 Identities = 16/42 (38%), Positives = 21/42 (50%)
 Frame = +1

Query: 307 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFIS 432
           +  W  +I GPP TPYE   Y   +     YP +PP  R I+
Sbjct: 34  ILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMIT 75


>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
           Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 155

 Score = 35.9 bits (79), Expect = 0.004
 Identities = 15/48 (31%), Positives = 23/48 (47%)
 Frame = +1

Query: 298 DMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 441
           D  L HW    +GP  + Y    +   ++    YP +PPT  F +RI+
Sbjct: 33  DDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIY 80


>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
           Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score = 34.3 bits (75), Expect = 0.012
 Identities = 14/47 (29%), Positives = 26/47 (55%)
 Frame = +1

Query: 334 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLV 474
           GP  TPY   ++ + +E  + YP + P+  F++RI    ++  +G V
Sbjct: 38  GPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSV 84


>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
           Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 32.7 bits (71), Expect = 0.037
 Identities = 18/74 (24%), Positives = 38/74 (51%)
 Frame = +1

Query: 220 RLLEELEQGQKGVGDGTISWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRY 399
           R+++E+E   +    G ++   E +    L ++   + GP ++ YE   + L++     Y
Sbjct: 6   RIIKEIETLTRDPPPGIVAAPTEDN----LRYFKITMEGPQQSAYEGGKFHLELFLPDEY 61

Query: 400 PDEPPTARFISRIH 441
           P  PP  RF+++I+
Sbjct: 62  PMMPPNVRFLTKIY 75


>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
           Pst2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1075

 Score = 31.1 bits (67), Expect = 0.11
 Identities = 20/64 (31%), Positives = 32/64 (50%), Gaps = 4/64 (6%)
 Frame = +1

Query: 205 CHGNFRLLEELEQGQKGVGDGTISWGLESDDDMTLTHW----TGMIIGPPRTPYENRMYS 372
           C  ++RLL   E+     G    +WG+ +DD ++   W    +G I+   +TPYE  M  
Sbjct: 337 CGPSYRLLPVEERNISCSGRDDFAWGILNDDWVSHPTWASEESGFIV-QRKTPYEEAMTK 395

Query: 373 LKIE 384
           L+ E
Sbjct: 396 LEEE 399


>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase
           kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1436

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = -3

Query: 504 SSKNWDLSIIHKSSLTIDTVHMNPRNKAGCWW 409
           S+ NW  S+++  SL  D +  NP+NK    W
Sbjct: 291 STVNWVQSVLNLPSLLSDDLMANPKNKERFEW 322


>SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 584

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 12/22 (54%), Positives = 16/22 (72%)
 Frame = -3

Query: 324 SSPMSKSHIIIAFKTPRNGSIA 259
           SSPMS+SH+ I+ + PR G  A
Sbjct: 439 SSPMSESHVQISSEMPRTGMSA 460


>SPBC215.01 ||SPBC3B9.20|GTPase activating
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 27.1 bits (57), Expect = 1.9
 Identities = 13/32 (40%), Positives = 20/32 (62%)
 Frame = -3

Query: 144 KLRLIQSSFIA*ALPKQIRFI*SKYGHLQQSN 49
           K RL++S F   +LP Q+RF+   Y +L+  N
Sbjct: 27  KFRLLESQFALYSLPCQLRFV--HYNNLEDPN 56


>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
           Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 177

 Score = 26.2 bits (55), Expect = 3.2
 Identities = 9/37 (24%), Positives = 19/37 (51%)
 Frame = +1

Query: 331 IGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 441
           I P    Y+   +  +I+    YP +PP  + +++I+
Sbjct: 58  IRPDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIY 94


>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 412

 Score = 25.4 bits (53), Expect = 5.7
 Identities = 17/51 (33%), Positives = 26/51 (50%)
 Frame = -3

Query: 348 CPWWANNHSSPMSKSHIIIAFKTPRNGSIAHSLLALFKFF*ETKIPVAPQR 196
           C  W +N  S  + S + + +++    S A    A+F+   ETKIPV P R
Sbjct: 24  CECWDHNLCSSQA-SEMDLIYQSQDTHSCASKQDAVFQLLSETKIPV-PNR 72


>SPAC12B10.04 |||tubulin-tyrosine ligase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 403

 Score = 25.0 bits (52), Expect = 7.5
 Identities = 13/47 (27%), Positives = 21/47 (44%)
 Frame = -3

Query: 540 LKNCFDGIVTLPSSKNWDLSIIHKSSLTIDTVHMNPRNKAGCWWLIR 400
           LKN    +    S+K  +        +  D V+ NP+ K  C ++IR
Sbjct: 27  LKNSGKELTFTNSAKEANFQWAQYEDIDFDEVYKNPKTKLCCSYVIR 73


>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1841

 Score = 25.0 bits (52), Expect = 7.5
 Identities = 16/49 (32%), Positives = 24/49 (48%)
 Frame = -2

Query: 334 Q*SFQSNE*ESYHHRFQDPKKWFHRPLPFGLVQVLLRDENSRGTTTPDD 188
           Q S QS++  +     Q P K FHRP P  L + + R  +   T+  D+
Sbjct: 785 QMSRQSSQPSTVSTILQIPDKKFHRPFPRHLHRYVGRSASESLTSEKDE 833


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 25.0 bits (52), Expect = 7.5
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = -1

Query: 287 SRPQEMVPSPTPFWPCSSSSKRRKFPWHHN 198
           S P  MV SPT +  CS++  +  FP  H+
Sbjct: 111 SHPSYMVQSPTSYHACSNN--QSPFPHSHH 138


>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1334

 Score = 24.6 bits (51), Expect = 9.9
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = -2

Query: 274 KWFHRPLPFGLVQVLLRDEN 215
           K F RP+P  L+Q+ L D+N
Sbjct: 890 KVFQRPIPLLLLQLFLVDDN 909


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,374,549
Number of Sequences: 5004
Number of extensions: 49628
Number of successful extensions: 139
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 231978230
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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