Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0699
(611 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 287 7e-79
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 282 2e-77
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 146 2e-36
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 103 2e-23
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.019
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch... 27 1.6
SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosacc... 25 6.5
SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch... 25 8.6
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 25 8.6
SPCC16C4.10 |||6-phosphogluconolactonase |Schizosaccharomyces po... 25 8.6
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 25 8.6
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 287 bits (705), Expect = 7e-79
Identities = 130/181 (71%), Positives = 154/181 (85%)
Frame = +2
Query: 68 KEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAI 247
KE++D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++
Sbjct: 116 KEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSV 175
Query: 248 YPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLI 427
YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI
Sbjct: 176 YPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLI 235
Query: 428 GQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKGYHEQLSVAEI 607
Q+VSSITASLRF G+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA K +HE SV EI
Sbjct: 236 AQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEI 295
Query: 608 T 610
T
Sbjct: 296 T 296
Score = 45.2 bits (102), Expect = 8e-06
Identities = 16/22 (72%), Positives = 22/22 (100%)
Frame = +1
Query: 4 RLITGKEDAANNYARGHYTIGE 69
+++TGKEDA+NNYARGHYT+G+
Sbjct: 95 QMVTGKEDASNNYARGHYTVGK 116
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 282 bits (692), Expect = 2e-77
Identities = 128/181 (70%), Positives = 153/181 (84%)
Frame = +2
Query: 68 KEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAI 247
KE+VD V D+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++
Sbjct: 112 KELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSV 171
Query: 248 YPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLI 427
YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI
Sbjct: 172 YPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLI 231
Query: 428 GQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKGYHEQLSVAEI 607
Q+VSSITASLRF+G+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA K +HE SV EI
Sbjct: 232 AQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEI 291
Query: 608 T 610
T
Sbjct: 292 T 292
Score = 46.4 bits (105), Expect = 3e-06
Identities = 18/22 (81%), Positives = 22/22 (100%)
Frame = +1
Query: 4 RLITGKEDAANNYARGHYTIGE 69
+LITGKEDA+NNYARGHYT+G+
Sbjct: 91 QLITGKEDASNNYARGHYTVGK 112
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 146 bits (354), Expect = 2e-36
Identities = 68/180 (37%), Positives = 106/180 (58%)
Frame = +2
Query: 71 EIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIY 250
E+ D VLD +R+ A+ C LQGF + H LL+ ++ +Y + F++
Sbjct: 111 ELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVA 170
Query: 251 PAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIG 430
PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVS 230
Query: 431 QIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKGYHEQLSVAEIT 610
+++ +T S RF G LN DL + N+VP+PR+HF +V +AP+ + + +SV E+T
Sbjct: 231 AVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELT 290
Score = 25.8 bits (54), Expect = 4.9
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +1
Query: 7 LITGKEDAANNYARGHYTIG 66
+I G+ A N +A+GHYT G
Sbjct: 90 IIYGQSGAGNIWAKGHYTEG 109
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 103 bits (248), Expect = 2e-23
Identities = 54/164 (32%), Positives = 87/164 (53%), Gaps = 1/164 (0%)
Frame = +2
Query: 68 KEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAI 247
+ I + ++D I + AD L+GF + H L+ERL+ Y KK +++
Sbjct: 112 ERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSV 171
Query: 248 YPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRL 424
+P Q VS VV+PYNS+L ++D ++DN A+ I L + PT+ N+L
Sbjct: 172 FPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQL 231
Query: 425 IGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAP 556
+ ++S+ T +LR+ G +N DL +L+P PR HF L +Y P
Sbjct: 232 VSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTP 275
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 33.9 bits (74), Expect = 0.019
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = -2
Query: 517 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 356
R + E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 27.5 bits (58), Expect = 1.6
Identities = 31/111 (27%), Positives = 46/111 (41%), Gaps = 8/111 (7%)
Frame = -2
Query: 550 VRDQWEVDTGVRHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAA 371
V D VDT V + +A ES R++ D S Q + E T A
Sbjct: 454 VADDDAVDTSVTATDDFD-ASASSSNAYESYRNTYTDDMDSSSIYQTSLSNVKTEETTPA 512
Query: 370 DV---IDGFIV-----DHESTVRVLKGCVGGEDRVVGLDDGSGNLRRGVDG 242
+ +DG ++ +HE V+ G E ++ DDGSG +R +DG
Sbjct: 513 EPASKVDGVVLYDFTGEHEG---VITASEGQEFTLLEPDDGSGWVRVKIDG 560
>SPAC6G9.04 |mug79||meiotically upregulated gene
Mug79|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 25.4 bits (53), Expect = 6.5
Identities = 15/58 (25%), Positives = 27/58 (46%)
Frame = -2
Query: 511 QVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDGFIVDHE 338
Q ++ VH ++A E R N + QVG +N ++ D+ + F+ D+E
Sbjct: 601 QSQIKSQSVHHENAAEEIRKMRNLRNSRINSQQVGEKVFVNPDVKTMDIQETFLQDYE 658
>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 25.0 bits (52), Expect = 8.6
Identities = 10/36 (27%), Positives = 18/36 (50%)
Frame = -2
Query: 139 ESLQTGTLVSELADSVQNQIYDFLLQWCSDHGHSCW 32
E+ + G + E A+ ++YDFL C+ + W
Sbjct: 127 EAYEGGPIDLERANVTNKEVYDFLQTACAKYNIGFW 162
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 25.0 bits (52), Expect = 8.6
Identities = 8/29 (27%), Positives = 14/29 (48%)
Frame = -2
Query: 118 LVSELADSVQNQIYDFLLQWCSDHGHSCW 32
L S+++ NQI + + WC + W
Sbjct: 1857 LYSQMSSDENNQILELIAGWCEHLAQNTW 1885
>SPCC16C4.10 |||6-phosphogluconolactonase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 257
Score = 25.0 bits (52), Expect = 8.6
Identities = 21/91 (23%), Positives = 40/91 (43%), Gaps = 2/91 (2%)
Frame = -2
Query: 301 EDRVVGLDDGSGNLRRGVDGELQFRLLAVVNGETLHQ*GSE--PRAGTSTEGVEDQESLQ 128
++R+V LDD + N + +L F + + +H E + V D+ Q
Sbjct: 68 DERIVPLDDENSNY--ALCKKLIFDKFEGFDPKKIHTINPELLKENPIDPQNVADEYEKQ 125
Query: 127 TGTLVSELADSVQNQIYDFLLQWCSDHGHSC 35
+ + + +V+ ++D LL C GH+C
Sbjct: 126 LVHVFAN-SSTVKVPVFDLLLLGCGPDGHTC 155
>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 25.0 bits (52), Expect = 8.6
Identities = 20/56 (35%), Positives = 26/56 (46%)
Frame = +2
Query: 413 LNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKGY 580
LNR+ QIV + + + +NV L E Q L RI + A AEKGY
Sbjct: 688 LNRIDDQIVFNKLSEKNLEDIVNVRLDEVQQRLND-RRIILTVTEAARKWLAEKGY 742
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,430,960
Number of Sequences: 5004
Number of extensions: 47892
Number of successful extensions: 172
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 267622334
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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