Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0680
(605 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase / lon... 71 6e-13
At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase, puta... 60 1e-09
At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase famil... 59 3e-09
At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase, puta... 56 2e-08
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 53 1e-07
At4g14070.1 68417.m02172 AMP-binding protein, putative similar t... 52 4e-07
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil... 50 1e-06
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil... 50 1e-06
At3g23790.1 68416.m02990 AMP-binding protein, putative similar t... 49 2e-06
At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase famil... 48 5e-06
At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein ... 46 2e-05
At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-c... 44 8e-05
At5g27600.1 68418.m03305 AMP-binding protein, putative similar t... 44 1e-04
At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein ... 44 1e-04
At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein ... 44 1e-04
At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase / lon... 43 1e-04
At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-c... 37 0.012
At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14) ident... 37 0.012
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl... 36 0.021
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl... 36 0.021
At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase fam... 33 0.15
At1g20500.1 68414.m02554 4-coumarate--CoA ligase family / 4-coum... 33 0.15
At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein ... 33 0.15
At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase fam... 33 0.19
At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase fam... 32 0.26
At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl... 31 0.59
At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) simil... 31 0.59
At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein ... 30 1.0
At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) simil... 30 1.0
At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl... 30 1.0
At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl... 30 1.0
At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) simil... 29 1.8
At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein ... 29 3.2
At1g21540.1 68414.m02694 AMP-binding protein, putative strong si... 28 4.2
At1g21530.1 68414.m02693 AMP-binding protein, putative strong si... 28 4.2
At3g28790.1 68416.m03593 expressed protein 28 5.5
At1g31812.1 68414.m03905 acyl-CoA binding protein / ACBP identic... 27 7.3
At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase fam... 27 7.3
At3g03570.1 68416.m00360 expressed protein similar to hypothetic... 27 9.6
At1g68600.1 68414.m07839 expressed protein contains Pfam profile... 27 9.6
At1g25480.1 68414.m03164 expressed protein contains Pfam profile... 27 9.6
>At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to
acyl-CoA synthetase (MF7P) from Brassica napus
[gi:1617270]
Length = 666
Score = 70.9 bits (166), Expect = 6e-13
Identities = 55/197 (27%), Positives = 92/197 (46%), Gaps = 12/197 (6%)
Frame = +1
Query: 49 RYPNATALATKK-ADGKWHKITYKQYQDRVRIIAK---AFLKLGLDRYHSVSILGFNSEQ 216
+YPN L ++ DGK K ++ YQ+ I+ K + +G+ I G NS +
Sbjct: 57 KYPNNPMLGRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPE 116
Query: 217 WFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDKKQLDKILSVKHKLPLL 396
W I+ T ADA + S +I V++KK + + + +
Sbjct: 117 WIISMEACNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYM 176
Query: 397 KAIVQWEG-------PVDTSIPGIYSWDQLLEIG-AKEPDTQLNEILKSIAVNECCTLVY 552
K +V + G +T IY+WD+ L++G K+ D + + KS + CT++Y
Sbjct: 177 KTVVSFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKK--KS----DICTIMY 230
Query: 553 TSGTVGPPKAVMLSHDN 603
TSGT G PK VM+S+++
Sbjct: 231 TSGTTGDPKGVMISNES 247
>At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase,
putative / long-chain acyl-CoA synthetase, putative
similar to acyl-CoA synthetase (MF7P) gi:1617270 from
Brassica napus
Length = 666
Score = 59.7 bits (138), Expect = 1e-09
Identities = 49/196 (25%), Positives = 88/196 (44%), Gaps = 11/196 (5%)
Frame = +1
Query: 49 RYPNATALATKK-ADGKWHKITYKQYQDRVRIIAK---AFLKLGLDRYHSVSILGFNSEQ 216
+YPN L ++ ++GK K +K Y++ I+ K + G+ I G N +
Sbjct: 57 KYPNNRMLGRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCE 116
Query: 217 WFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDKKQLDKILSVKHKLPLL 396
W I+ T A A + + +I V++KK + + + +
Sbjct: 117 WIISMEACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYM 176
Query: 397 KAIVQWEG-----PVDTSIPG--IYSWDQLLEIGAKEPDTQLNEILKSIAVNECCTLVYT 555
K +V + G + G I+SWD+ L++G + Q +K ++ CT++YT
Sbjct: 177 KTVVSFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGK---QYELPIKK--PSDICTIMYT 231
Query: 556 SGTVGPPKAVMLSHDN 603
SGT G PK VM+S+++
Sbjct: 232 SGTTGDPKGVMISNES 247
>At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
similar to GI:1617270 (MF7P) and gi:1617628 (MF45P) from
[Brassica napus] ; contains Pfam AMP-binding enzyme
domain PF00501
Length = 660
Score = 58.8 bits (136), Expect = 3e-09
Identities = 58/202 (28%), Positives = 88/202 (43%), Gaps = 12/202 (5%)
Frame = +1
Query: 28 LLSRTTARYPNATALATKK-AD---GKWHKITYKQYQDRVRIIAKAFLKLGLDRYHSVSI 195
+ S++ ++P+ L ++ D G + TYK+ + V I A G + V I
Sbjct: 47 IFSKSVEKFPDNNMLGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGI 106
Query: 196 LGFNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDKKQLDKILSV 375
G N QW IA T + A + +E + + VQD K + +L
Sbjct: 107 YGVNCPQWIIAMEACAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTK-IKGLLEP 165
Query: 376 KHKLPL-LKAIVQWEGPVD-----TSIPGI--YSWDQLLEIGAKEPDTQLNEILKSIAVN 531
K LKAIV + D S G+ YSW L +G ++P+ + A N
Sbjct: 166 DCKCAKRLKAIVSFTNVSDELSHKASEIGVKTYSWIDFLHMGREKPE----DTNPPKAFN 221
Query: 532 ECCTLVYTSGTVGPPKAVMLSH 597
CT++YTSGT G PK V+L+H
Sbjct: 222 -ICTIMYTSGTSGDPKGVVLTH 242
>At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase,
putative / long-chain acyl-CoA synthetase, putative
similar to GI:1617270 (MF7P) from [Brassica napus]
Length = 665
Score = 55.6 bits (128), Expect = 2e-08
Identities = 49/208 (23%), Positives = 92/208 (44%), Gaps = 11/208 (5%)
Frame = +1
Query: 13 ISVPGLLSRTTARYPNATALATKK-ADGKWHKITYKQYQDRVRIIAK---AFLKLGLDRY 180
+S + + + PN L ++ DGK K ++ Y++ ++ K + +G+ +
Sbjct: 45 VSAWDIFRLSVEKSPNNPMLGRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKG 104
Query: 181 HSVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDKKQLD 360
I G NS +W I+ T A A + + ++ ++ K +
Sbjct: 105 DKCGIYGANSPEWIISMEACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISE 164
Query: 361 KILSVKHKLPLLKAIVQW-EGPVDTSIPG------IYSWDQLLEIGAKEPDTQLNEILKS 519
+ + LK IV + E + + IYSWDQ L++G + +L E +S
Sbjct: 165 LLKTAPKSTKYLKYIVSFGEVTNNQRVEAERHRLTIYSWDQFLKLGEGK-HYELPEKRRS 223
Query: 520 IAVNECCTLVYTSGTVGPPKAVMLSHDN 603
+ CT++YTSGT G PK V+L++++
Sbjct: 224 ----DVCTIMYTSGTTGDPKGVLLTNES 247
>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to acyl
CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
Length = 665
Score = 53.2 bits (122), Expect = 1e-07
Identities = 51/200 (25%), Positives = 81/200 (40%), Gaps = 12/200 (6%)
Frame = +1
Query: 34 SRTTARYPNATALA----TKKADGKWHKITYKQYQDRVRIIAKAFLKLGLDRYHSVSILG 201
S +YPN L T G + ITYK+ D I A G+D H I G
Sbjct: 52 SEAVKKYPNEQMLGQRVTTDSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYG 111
Query: 202 FNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDKKQLDKILSVKH 381
N +W IA + +A + + ++ VQ+K + K
Sbjct: 112 ANCPEWIIAMEACMSQGITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKG 171
Query: 382 KLPLLKAIVQWEGPVDTS--------IPGIYSWDQLLEIGAKEPDTQLNEILKSIAVNEC 537
LK IV + G V ++ ++SW++ +G + + L K+ +
Sbjct: 172 CSSNLKTIVSF-GEVSSTQKEEAKNQCVSLFSWNEFSLMGNLD-EANLPRKRKT----DI 225
Query: 538 CTLVYTSGTVGPPKAVMLSH 597
CT++YTSGT G PK V+L++
Sbjct: 226 CTIMYTSGTTGEPKGVILNN 245
>At4g14070.1 68417.m02172 AMP-binding protein, putative similar to
AMP-binding protein [gi:1617272] from Brassica napus;
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA acyl-CoA synthetase-like protein
GI:20799730
Length = 727
Score = 51.6 bits (118), Expect = 4e-07
Identities = 48/210 (22%), Positives = 87/210 (41%), Gaps = 14/210 (6%)
Frame = +1
Query: 16 SVPGLLSRTTARYPNATALATKKADGKWHKITYKQYQDRVRIIAKAFLKLGLDRYHSVSI 195
+VP + + +Y + AL D K+TYKQ + + A+ LG+ +++
Sbjct: 98 AVPDIWRSSAEKYGDRVALVDPYHDPPL-KLTYKQLEQEILDFAEGLRVLGVKADEKIAL 156
Query: 196 LGFNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDKKQLDKIL-S 372
NS +W ++D + +S + S + V + + ++I S
Sbjct: 157 FADNSCRWLVSDQGIMATGAVNVVRGSRSSVEELLQIYRHSESVAIVVDNPEFFNRIAES 216
Query: 373 VKHKLPLLKAIVQWEGPVDTSIPG----IYSWDQLLEIGAKEP-------DTQL--NEIL 513
K L I+ W G +YS+ +++ G + DT+ N+ +
Sbjct: 217 FTSKASLRFLILLWGEKSSLVTQGMQIPVYSYAEIINQGQESRAKLSASNDTRSYRNQFI 276
Query: 514 KSIAVNECCTLVYTSGTVGPPKAVMLSHDN 603
S ++ ++YTSGT G PK VML+H N
Sbjct: 277 DS---DDTAAIMYTSGTTGNPKGVMLTHRN 303
>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 50.0 bits (114), Expect = 1e-06
Identities = 42/174 (24%), Positives = 75/174 (43%), Gaps = 4/174 (2%)
Frame = +1
Query: 94 KWHKITYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXXXX 273
KW +Y + +RV A + +G + V+I +WFIA
Sbjct: 128 KWQ--SYGEVFERVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQSITVVTIY 185
Query: 274 TTNSADACFHCLESSRANICAVQDKKQLDKILSVKHKLPLLKAIVQWEGP-VDTSIPGIY 450
+ +A + L +R + + D KQL K+ +++ L +K I+ E VD + +
Sbjct: 186 ASLGEEALIYSLNETRVSTL-ICDSKQLKKLSAIQSSLKTVKNIIYIEEDGVDVASSDVN 244
Query: 451 SWDQLLEIGAKEPDT--QLNEILKSI-AVNECCTLVYTSGTVGPPKAVMLSHDN 603
S + E + Q N + + + N +++TSG+ G PK VM++H N
Sbjct: 245 SMGDITVSSISEVEKLGQKNAVQPILPSKNGVAVIMFTSGSTGLPKGVMITHGN 298
>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 50.0 bits (114), Expect = 1e-06
Identities = 42/174 (24%), Positives = 75/174 (43%), Gaps = 4/174 (2%)
Frame = +1
Query: 94 KWHKITYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXXXX 273
KW +Y + +RV A + +G + V+I +WFIA
Sbjct: 128 KWQ--SYGEVFERVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQSITVVTIY 185
Query: 274 TTNSADACFHCLESSRANICAVQDKKQLDKILSVKHKLPLLKAIVQWEGP-VDTSIPGIY 450
+ +A + L +R + + D KQL K+ +++ L +K I+ E VD + +
Sbjct: 186 ASLGEEALIYSLNETRVSTL-ICDSKQLKKLSAIQSSLKTVKNIIYIEEDGVDVASSDVN 244
Query: 451 SWDQLLEIGAKEPDT--QLNEILKSI-AVNECCTLVYTSGTVGPPKAVMLSHDN 603
S + E + Q N + + + N +++TSG+ G PK VM++H N
Sbjct: 245 SMGDITVSSISEVEKLGQKNAVQPILPSKNGVAVIMFTSGSTGLPKGVMITHGN 298
>At3g23790.1 68416.m02990 AMP-binding protein, putative similar to
AMP-binding protein GB:CAA96521 from [Brassica napus]
(Plant Mol. Biol. (1997) 33 (5), 911-922); contains Pfam
AMP-binding enzyme domain PF00501; identical to cDNA
acyl-CoA synthetase-like protein GI:20799732
Length = 722
Score = 49.2 bits (112), Expect = 2e-06
Identities = 45/208 (21%), Positives = 85/208 (40%), Gaps = 12/208 (5%)
Frame = +1
Query: 16 SVPGLLSRTTARYPNATALATKKADGKWHKITYKQYQDRVRIIAKAFLKLGLDRYHSVSI 195
SVP + + +Y + A+ D TY+Q + + + +G+ +++
Sbjct: 82 SVPDIWRSSVEKYGDRVAVVDPYHDPP-STFTYRQLEQEILDFVEGLRVVGVKADEKIAL 140
Query: 196 LGFNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDKKQLDKIL-S 372
NS +W +AD + +S + S + V + + ++I S
Sbjct: 141 FADNSCRWLVADQGIMATGAVNVVRGSRSSVEELLQIYCHSESVALVVDNPEFFNRIAES 200
Query: 373 VKHKLPLLKAIVQWEGPVDTSIPG----IYSWDQLLEIG-------AKEPDTQLNEILKS 519
+K I+ W G +YS++++ + G A+ D+ E +
Sbjct: 201 FSYKAAPKFVILLWGEKSSLVTAGRHTPVYSYNEIKKFGQERRAKFARSNDSGKYEY-EY 259
Query: 520 IAVNECCTLVYTSGTVGPPKAVMLSHDN 603
I ++ T++YTSGT G PK VML+H N
Sbjct: 260 IDPDDIATIMYTSGTTGNPKGVMLTHQN 287
>At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS9) similar to LACS 3 [SP|O95573] from Homo Sapiens,
LACS 3 [SP|Q63151] from Rattus norvegicus; contains Pfam
HMM hit: AMP-binding enzymes PF00501
Length = 691
Score = 48.0 bits (109), Expect = 5e-06
Identities = 35/174 (20%), Positives = 75/174 (43%), Gaps = 3/174 (1%)
Frame = +1
Query: 91 GKWHKITYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXXX 270
G + +T+ + + V A +++G V+I E+WFI+
Sbjct: 102 GDYEWLTFGKTLEAVCDFASGLVQIGHKTEERVAIFADTREEWFISLQGCFRRNVTVVTI 161
Query: 271 XTTNSADACFHCLESSRANICAVQDKKQLDKILSVKHKLPLLKAIVQWEGPVDTSIPGIY 450
++ +A H L + + K+L K++ + +L +K ++ + + + +
Sbjct: 162 YSSLGEEALCHSLNETEVTT-VICGSKELKKLMDISQQLETVKRVICMDDEFPSDVNSNW 220
Query: 451 ---SWDQLLEIGAKEPDTQLNEILKSIAVNECCTLVYTSGTVGPPKAVMLSHDN 603
S+ + ++G + P + +AV ++YTSG+ G PK VM++H N
Sbjct: 221 MATSFTDVQKLGRENPVDPNFPLSADVAV-----IMYTSGSTGLPKGVMMTHGN 269
>At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
gi:112801 from Petroselinum crispum, GB:AAD40664 from
[Solanum tuberosum] (J. Biol. Chem. 266 (13), 8551-8559
(1991)); contains Pfam AMP-binding enzyme domain PF00501
Length = 542
Score = 46.0 bits (104), Expect = 2e-05
Identities = 25/59 (42%), Positives = 33/59 (55%)
Frame = +1
Query: 427 DTSIPGIYSWDQLLEIGAKEPDTQLNEILKSIAVNECCTLVYTSGTVGPPKAVMLSHDN 603
+ I G +W LLE G K DT EIL++ + C L ++SGT G K VML+H N
Sbjct: 151 EEKIEGAVNWKDLLEAGDKCGDTDNEEILQT----DLCALPFSSGTTGLQKGVMLTHRN 205
>At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501; acyl-activating
enzyme superfamily; identical to cDNA 4-coumarate-CoA
ligase-like protein (At4g05160) GI:29893226
Length = 544
Score = 44.0 bits (99), Expect = 8e-05
Identities = 47/196 (23%), Positives = 81/196 (41%)
Frame = +1
Query: 16 SVPGLLSRTTARYPNATALATKKADGKWHKITYKQYQDRVRIIAKAFLKLGLDRYHSVSI 195
S+ L R ++ YP+ A+A +T+ Q + V +A F +LG+ + V I
Sbjct: 28 SLVSFLFRNSSSYPSKLAIADSDTGDS---LTFSQLKSAVARLAHGFHRLGIRKNDVVLI 84
Query: 196 LGFNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDKKQLDKILSV 375
NS Q+ + L + + ++ S I + + DKI
Sbjct: 85 FAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKII-ISVNQLFDKIKGF 143
Query: 376 KHKLPLLKAIVQWEGPVDTSIPGIYSWDQLLEIGAKEPDTQLNEILKSIAVNECCTLVYT 555
+ LL + E P ++ I S+D ++E+ + EI +S + L+Y+
Sbjct: 144 DLPVVLLGSKDTVEIPPGSNSK-ILSFDNVMELSEPVSEYPFVEIKQS----DTAALLYS 198
Query: 556 SGTVGPPKAVMLSHDN 603
SGT G K V L+H N
Sbjct: 199 SGTTGTSKGVELTHGN 214
>At5g27600.1 68418.m03305 AMP-binding protein, putative similar to
AMP-binding protein (MF39P) gi:1617274 from Brassica
napus, long-chain-fatty-acid--CoA ligase - Brassica
napus, EMBL:Z72152; contains Pfam AMP-binding enzyme
domain PF00501
Length = 700
Score = 43.6 bits (98), Expect = 1e-04
Identities = 41/175 (23%), Positives = 74/175 (42%), Gaps = 4/175 (2%)
Frame = +1
Query: 91 GKWHKITYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXXX 270
G++ +TY + + I L G+++ V + N +W + D
Sbjct: 114 GEYSWMTYGEAASERQAIGSGLLFHGVNQGDCVGLYFINRPEWLVVDHACAAYSFVSVPL 173
Query: 271 XTTNSADACFHCLESSRANICAVQDKKQ-LDKILSVKHKLPLLKAIVQWEGPVDTSIPGI 447
T DA + + AN+ A+ Q L+ +LS ++P ++ IV G D +P +
Sbjct: 174 YDTLGPDAVKFVV--NHANLQAIFCVPQTLNILLSFLAEIPSIRLIVV-VGGADEHLPSL 230
Query: 448 YSWDQLLEIGAKEPDTQLNEILKSIAV---NECCTLVYTSGTVGPPKAVMLSHDN 603
+ + ++ +Q L + + T+ YTSGT G PK V+L+H N
Sbjct: 231 PRGTGVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGN 285
>At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 473
Score = 43.6 bits (98), Expect = 1e-04
Identities = 40/166 (24%), Positives = 75/166 (45%)
Frame = +1
Query: 106 ITYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNS 285
+T+ + V +A ++G+ + H V +L NS + + L N+
Sbjct: 58 LTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNT 117
Query: 286 ADACFHCLESSRANICAVQDKKQLDKILSVKHKLPLLKAIVQWEGPVDTSIPGIYSWDQL 465
++ ++ S + A + L KI + KLP+ ++ E VD+ + +L
Sbjct: 118 SNEIAKQIKDSNP-VLAFTTSQLLPKISAAAKKLPI---VLMDEERVDS----VGDVRRL 169
Query: 466 LEIGAKEPDTQLNEILKSIAVNECCTLVYTSGTVGPPKAVMLSHDN 603
+E+ KEP N + + + ++ TL+Y+SGT G K V+ SH N
Sbjct: 170 VEMMKKEPSG--NRVKERVDQDDTATLLYSSGTTGMSKGVISSHRN 213
>At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 546
Score = 43.6 bits (98), Expect = 1e-04
Identities = 40/166 (24%), Positives = 75/166 (45%)
Frame = +1
Query: 106 ITYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNS 285
+T+ + V +A ++G+ + H V +L NS + + L N+
Sbjct: 58 LTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNT 117
Query: 286 ADACFHCLESSRANICAVQDKKQLDKILSVKHKLPLLKAIVQWEGPVDTSIPGIYSWDQL 465
++ ++ S + A + L KI + KLP+ ++ E VD+ + +L
Sbjct: 118 SNEIAKQIKDSNP-VLAFTTSQLLPKISAAAKKLPI---VLMDEERVDS----VGDVRRL 169
Query: 466 LEIGAKEPDTQLNEILKSIAVNECCTLVYTSGTVGPPKAVMLSHDN 603
+E+ KEP N + + + ++ TL+Y+SGT G K V+ SH N
Sbjct: 170 VEMMKKEPSG--NRVKERVDQDDTATLLYSSGTTGMSKGVISSHRN 213
>At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase (LACS6) strong similarity
to AMP-binding protein (MF39P) gi:1617274 from Brassica
napus, similar to putative long-chain-fatty-acid--CoA
ligase (brain isozyme) GB:P33124 [Rattus norvegicus];
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA AtLACS6 for long-chain acyl-CoA
synthetase GI:22531705
Length = 701
Score = 43.2 bits (97), Expect = 1e-04
Identities = 45/181 (24%), Positives = 72/181 (39%), Gaps = 9/181 (4%)
Frame = +1
Query: 88 DGKWHKITYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXX 267
D KW +TY + + + G+ SV I N +W I D
Sbjct: 115 DYKW--MTYGEAGTARTALGSGLVHHGIPMGSSVGIYFINRPEWLIVDHACSSYSYVSVP 172
Query: 268 XXTTNSADACFHCLESS--RANICAVQDKKQLDKILSVKHKLPLLKAIVQWEGPVDT--S 435
T DA + + +A C + L+ +LS ++P ++ +V G +++ S
Sbjct: 173 LYDTLGPDAVKFIVNHATVQAIFCVAET---LNSLLSCLSEMPSVRLVVVVGGLIESLPS 229
Query: 436 IPG-----IYSWDQLLEIGAKEPDTQLNEILKSIAVNECCTLVYTSGTVGPPKAVMLSHD 600
+P + S+ LL G P +A T+ YTSGT G PK V+L+H
Sbjct: 230 LPSSSGVKVVSYSVLLNQGRSNPQRFFPPKPDDVA-----TICYTSGTTGTPKGVVLTHA 284
Query: 601 N 603
N
Sbjct: 285 N 285
>At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2
[gi:12229665] and 4CL1 [gi:12229649] from [Arabidopsis
thaliana], 4CL1 [gi:12229631] from Nicotiana tabacum
Length = 570
Score = 36.7 bits (81), Expect = 0.012
Identities = 42/167 (25%), Positives = 74/167 (44%), Gaps = 3/167 (1%)
Frame = +1
Query: 106 ITYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNS 285
+TY Q +R IA +LG+ V +L NS ++ ++ L +
Sbjct: 74 LTYADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYT 133
Query: 286 ADACFHCLESSRANICAVQDKKQLDKILSVKHKLPLLKAIVQW--EGPVDTSIPGIYSWD 459
++S A + + K +DK+ ++K+ L+ + G V +S G S+
Sbjct: 134 QPEIAKQAKASAAKMI-ITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFT 192
Query: 460 QLLEIGAKEPDTQLNEILK-SIAVNECCTLVYTSGTVGPPKAVMLSH 597
+L + A E E+LK I+ + + Y+SGT G PK VM++H
Sbjct: 193 ELTQ--ADE-----TELLKPKISPEDTVAMPYSSGTTGLPKGVMITH 232
>At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14)
identical to acyl-activating enzyme 14 [Arabidopsis
thaliana]; similar to SP|Q42524 4-coumarate--CoA ligase
1 (EC 6.2.1.12) (4-coumaroyl-CoA synthase 1)
{Arabidopsis thaliana}; contains Pfam profile PF00501:
AMP-binding enzyme; identical to cDNA acyl-activating
enzyme 14 (At1g30520) GI:29893263
Length = 560
Score = 36.7 bits (81), Expect = 0.012
Identities = 42/170 (24%), Positives = 70/170 (41%), Gaps = 5/170 (2%)
Frame = +1
Query: 103 KITYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSE---QWFIADLXXXXXXXXXXXXX 273
K T +++ D V +A ++LGL VSI FNS+ +W +A
Sbjct: 32 KRTGREFVDGVLSLAAGLIRLGLRNGDVVSIAAFNSDLFLEWLLAVALVGGVVAPLNYRW 91
Query: 274 TTNSADACFHCLESSRANICAVQDKKQLDKILSVKH-KLPLLKAIVQWEGPVDTSIPGIY 450
+ A +E + V D+ + + V++ +P LK V E TS
Sbjct: 92 SLKEAKMAMLLVEP----VLLVTDETCVSWCIDVQNGDIPSLKWRVLME---STSTDFAN 144
Query: 451 SWDQLLEIGAKEPDTQLNEILK-SIAVNECCTLVYTSGTVGPPKAVMLSH 597
+Q L + T + + + A ++ + +TSGT G PK V +SH
Sbjct: 145 ELNQFLTTEMLKQRTLVPSLATYAWASDDAVVICFTSGTTGRPKGVTISH 194
>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 490
Score = 35.9 bits (79), Expect = 0.021
Identities = 48/201 (23%), Positives = 87/201 (43%), Gaps = 6/201 (2%)
Frame = +1
Query: 13 ISVPGLLSRTTARYPNATALATKKA--DGKW-HKITYKQYQDRVRIIAKAFLKLGLDRYH 183
I +P LS + N + ATK +G H TY R IA F KLG+++
Sbjct: 32 IYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFHKLGVNQND 91
Query: 184 SVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDKKQLDK 363
V +L N ++ ++ L + ++S + + + + +DK
Sbjct: 92 VVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLI-ITEARYVDK 150
Query: 364 ILSVKHKLPLLKAIVQWEGPVDTSIP-GIYSWDQLLEIGAKEPDTQLNEILKSIAVN--E 534
I +++ ++ IV + IP G + +L + T+ +E++ S+ ++ +
Sbjct: 151 IKPLQNDDGVV--IVCIDDNESVPIPEGCLRFTELTQ-----STTEASEVIDSVEISPDD 203
Query: 535 CCTLVYTSGTVGPPKAVMLSH 597
L Y+SGT G PK VML+H
Sbjct: 204 VVALPYSSGTTGLPKGVMLTH 224
>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 561
Score = 35.9 bits (79), Expect = 0.021
Identities = 48/201 (23%), Positives = 87/201 (43%), Gaps = 6/201 (2%)
Frame = +1
Query: 13 ISVPGLLSRTTARYPNATALATKKA--DGKW-HKITYKQYQDRVRIIAKAFLKLGLDRYH 183
I +P LS + N + ATK +G H TY R IA F KLG+++
Sbjct: 32 IYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFHKLGVNQND 91
Query: 184 SVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDKKQLDK 363
V +L N ++ ++ L + ++S + + + + +DK
Sbjct: 92 VVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLI-ITEARYVDK 150
Query: 364 ILSVKHKLPLLKAIVQWEGPVDTSIP-GIYSWDQLLEIGAKEPDTQLNEILKSIAVN--E 534
I +++ ++ IV + IP G + +L + T+ +E++ S+ ++ +
Sbjct: 151 IKPLQNDDGVV--IVCIDDNESVPIPEGCLRFTELTQ-----STTEASEVIDSVEISPDD 203
Query: 535 CCTLVYTSGTVGPPKAVMLSH 597
L Y+SGT G PK VML+H
Sbjct: 204 VVALPYSSGTTGLPKGVMLTH 224
>At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 535
Score = 33.1 bits (72), Expect = 0.15
Identities = 43/206 (20%), Positives = 82/206 (39%), Gaps = 7/206 (3%)
Frame = +1
Query: 1 HEAPISVPGLLSRTTARYPNATALATKKADGKWHKITYKQYQDRVRIIAKAFLKLGLDRY 180
+ P++ L R + YPN T++ + + T+ Q DR +A + + L + +
Sbjct: 10 NNVPLTPITFLKRASECYPNRTSIIYGQT-----RFTWPQTYDRCCRLAASLISLNIAKN 64
Query: 181 HSVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDK---- 348
VS++ N+ + T A + L ++ I +
Sbjct: 65 DVVSVVAPNTPAIYEMHFAVPMAGAVLNPINTRLDATSITTILRHAQPKILFIHRNFEPL 124
Query: 349 -KQLDKILSVKH-KLPLLKAIVQWEGPVDTSIPGIYSWDQLLEIGAKEPDTQLNEILKSI 522
+++ +LS +L LL + ++ L+++G EP + L E + I
Sbjct: 125 AREILHLLSCDDLQLNLLVIFIDEYNSAKRVSSEELDYESLIQMG--EPTSPLVENMFRI 182
Query: 523 A-VNECCTLVYTSGTVGPPKAVMLSH 597
+ +L YTSGT PK V++SH
Sbjct: 183 QNEQDPISLNYTSGTTADPKGVVISH 208
>At1g20500.1 68414.m02554 4-coumarate--CoA ligase family /
4-coumaroyl-CoA synthase family similar to SP|Q42524 and
SP|Q9S725; contains Pfam AMP-binding enzyme domain
PF00501
Length = 412
Score = 33.1 bits (72), Expect = 0.15
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = +1
Query: 463 LLEIGAKEPDTQLNEILKSIAVNECCTLVYTSGTVGPPKAVMLSHDN 603
L E+ KEP Q + + ++ ++Y+SGT GP K V+ SH N
Sbjct: 177 LSEMVKKEPSGQ--RVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRN 221
>At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|Q9S725 from Arabidopsis thaliana and SP|P17814 from
Oryza sativa; contains Pfam AMP-binding enzyme domain
PF00501
Length = 565
Score = 33.1 bits (72), Expect = 0.15
Identities = 41/162 (25%), Positives = 70/162 (43%), Gaps = 5/162 (3%)
Frame = +1
Query: 133 VRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLE 312
V +A LG+ + + V IL NS + I L N++D +
Sbjct: 84 VERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIG 143
Query: 313 SSRANICAVQDKKQLDKILSVKH-KLPLLKAIVQWEGPVDTSIPGIYSWDQLLEIGAKEP 489
SR + A K + K+ + + LP +V + D +P D++ +G E
Sbjct: 144 DSRP-VLAFTTCKLVSKLAAASNFNLP----VVLMD---DYHVPSQSYGDRVKLVGRLET 195
Query: 490 --DTQLNE--ILKSIAVNECCTLVYTSGTVGPPKAVMLSHDN 603
+T+ +E + + + ++ L+Y+SGT G K VMLSH N
Sbjct: 196 MIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRN 237
>At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 580
Score = 32.7 bits (71), Expect = 0.19
Identities = 44/207 (21%), Positives = 83/207 (40%), Gaps = 8/207 (3%)
Frame = +1
Query: 1 HEAPISVPGLLSRTTARYPNATALATKKADGKWHKITYKQYQDRVRIIAKAFLKLGLDRY 180
+ P++ L R + YPN T++ K + T+ Q DR +A + + L + +
Sbjct: 10 NNVPLTPMTFLKRASECYPNRTSIIYGKT-----RFTWPQTYDRCCRLAASLISLNISKN 64
Query: 181 HSVSILGFNS----EQWFIADLXXXXXXXXXXXXXTTNSADACFHC----LESSRANICA 336
VS++ N+ E F + T+ A H L R+
Sbjct: 65 DVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRSFEAL 124
Query: 337 VQDKKQLDKILSVKHKLPLLKAIVQWEGPVDTSIPGIYSWDQLLEIGAKEPDTQLNEILK 516
++ L LP++ I + + P S + ++ L++ G P + + + +
Sbjct: 125 ARESLHLLSSEDSNLNLPVI-FIHENDFPKRASFEEL-DYECLIQRGEPTP-SMVARMFR 181
Query: 517 SIAVNECCTLVYTSGTVGPPKAVMLSH 597
++ +L YTSGT PK V++SH
Sbjct: 182 IQDEHDPISLNYTSGTTADPKGVVISH 208
>At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 2 AMPBP2 (AMPBP2) GI:20799712
Length = 603
Score = 32.3 bits (70), Expect = 0.26
Identities = 19/50 (38%), Positives = 27/50 (54%)
Frame = +1
Query: 448 YSWDQLLEIGAKEPDTQLNEILKSIAVNECCTLVYTSGTVGPPKAVMLSH 597
YS+D E K D++ EI+K + ++ YTSGT PK V+ SH
Sbjct: 215 YSFDYEYETLLKSGDSEF-EIIKPRCEWDPISINYTSGTTSRPKGVVYSH 263
>At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 /
4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725
4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
(4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana}
Length = 556
Score = 31.1 bits (67), Expect = 0.59
Identities = 13/36 (36%), Positives = 23/36 (63%)
Frame = +1
Query: 490 DTQLNEILKSIAVNECCTLVYTSGTVGPPKAVMLSH 597
+ +++ I + I+ + L ++SGT G PK VML+H
Sbjct: 182 EPRVDSIPEKISPEDVVALPFSSGTTGLPKGVMLTH 217
>At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 11 (At1g66120) GI:29893230,
acyl-activating enzyme 11 [Arabidopsis thaliana]
GI:29893231
Length = 572
Score = 31.1 bits (67), Expect = 0.59
Identities = 43/205 (20%), Positives = 78/205 (38%), Gaps = 6/205 (2%)
Frame = +1
Query: 1 HEAPISVPGLLSRTTARYPNATALATKKADGKWHKITYKQYQDRVRIIAKAFLKLGLDRY 180
+ P++ L R + YPN T++ + + T+ Q DR +A + L L + R
Sbjct: 10 NNVPLTPITFLKRASECYPNRTSIIYGQT-----RFTWPQTYDRCCRLAASLLSLNITRN 64
Query: 181 HSVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDK---- 348
VSIL N + T A L + I V +
Sbjct: 65 DVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYEFAPL 124
Query: 349 -KQLDKILSVKHKLPLLKAIVQWEGPVDTS-IPGIYSWDQLLEIGAKEPDTQLNEILKSI 522
+++ +++ P + I+ E T ++ L+ G P + + + +
Sbjct: 125 IQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSAS-MFRVH 183
Query: 523 AVNECCTLVYTSGTVGPPKAVMLSH 597
++ +L YTSGT PK V++SH
Sbjct: 184 NEHDPISLNYTSGTTADPKGVVISH 208
>At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2,
Arabidopsis thaliana [gi:12229665], 4CL1, Nicotiana
tabacum [gi:12229631]; contains Pfam AMP-binding enzyme
domain PF00501
Length = 550
Score = 30.3 bits (65), Expect = 1.0
Identities = 17/47 (36%), Positives = 24/47 (51%)
Frame = +1
Query: 463 LLEIGAKEPDTQLNEILKSIAVNECCTLVYTSGTVGPPKAVMLSHDN 603
L E+ KEP Q + + ++ L+Y+SGT G K V SH N
Sbjct: 179 LTEMMKKEPSGQA--VRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGN 223
>At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 12 (At1g65890) mRNA
GI:29893228, acyl-activating enzyme 12 [Arabidopsis
thaliana] GI:29893229
Length = 578
Score = 30.3 bits (65), Expect = 1.0
Identities = 42/205 (20%), Positives = 80/205 (39%), Gaps = 6/205 (2%)
Frame = +1
Query: 1 HEAPISVPGLLSRTTARYPNATALATKKADGKWHKITYKQYQDRVRIIAKAFLKLGLDRY 180
+ P++ L R + YPN T++ K + T+ Q DR +A + + L + +
Sbjct: 10 NNVPLTPITFLKRASECYPNRTSIIYGKT-----RFTWPQTYDRCCRLAASLISLNIGKN 64
Query: 181 HSVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNSADACFHCLESSRANICAVQDK---- 348
VS++ N+ + T A + L ++ I +
Sbjct: 65 DVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRSFEPL 124
Query: 349 -KQLDKILSVKHKLPLLKAIVQWEGPVDTSIPGIYS-WDQLLEIGAKEPDTQLNEILKSI 522
+++ ++LS + L I E + S ++ L++ G P L +
Sbjct: 125 AREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTP-LLLARMFCIQ 183
Query: 523 AVNECCTLVYTSGTVGPPKAVMLSH 597
++ +L YTSGT PK V++SH
Sbjct: 184 DEHDPISLNYTSGTTADPKGVVISH 208
>At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 495
Score = 30.3 bits (65), Expect = 1.0
Identities = 34/165 (20%), Positives = 67/165 (40%), Gaps = 2/165 (1%)
Frame = +1
Query: 109 TYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNSA 288
TY + R +A KLG+ + + IL NS ++ + + ++
Sbjct: 77 TYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTS 136
Query: 289 DACFHCLESSRANICAVQDKKQLDKILSVKHKLPLLKAIVQWEGPVDTSIPGIYSWDQLL 468
+ L+SS A + + + +DK+ ++ L L+ D P + L
Sbjct: 137 QELYKQLKSSGAKLI-ITHSQYVDKLKNLGENLTLITT--------DEPTP-----ENCL 182
Query: 469 EIGAKEPDTQLNEILKSIAV--NECCTLVYTSGTVGPPKAVMLSH 597
D + N +++ + ++ L ++SGT G PK V+L+H
Sbjct: 183 PFSTLITDDETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTH 227
>At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 561
Score = 30.3 bits (65), Expect = 1.0
Identities = 34/165 (20%), Positives = 67/165 (40%), Gaps = 2/165 (1%)
Frame = +1
Query: 109 TYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXXXXTTNSA 288
TY + R +A KLG+ + + IL NS ++ + + ++
Sbjct: 77 TYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTS 136
Query: 289 DACFHCLESSRANICAVQDKKQLDKILSVKHKLPLLKAIVQWEGPVDTSIPGIYSWDQLL 468
+ L+SS A + + + +DK+ ++ L L+ D P + L
Sbjct: 137 QELYKQLKSSGAKLI-ITHSQYVDKLKNLGENLTLITT--------DEPTP-----ENCL 182
Query: 469 EIGAKEPDTQLNEILKSIAV--NECCTLVYTSGTVGPPKAVMLSH 597
D + N +++ + ++ L ++SGT G PK V+L+H
Sbjct: 183 PFSTLITDDETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTH 227
>At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) similar
to malonyl CoA synthetase GB:AAF28840 from
[Bradyrhizobium japonicum]; contains Pfam AMP-binding
enzyme domain PF00501; identical to cDNA acyl-activating
enzyme 13 (At3g16170) GI:29893232, acyl-activating
enzyme 13 [Arabidopsis thaliana] GI:29893233
Length = 544
Score = 29.5 bits (63), Expect = 1.8
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = +1
Query: 544 LVYTSGTVGPPKAVMLSHDN 603
+VYTSGT G PK V+ +H++
Sbjct: 175 IVYTSGTTGKPKGVVHTHNS 194
>At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL
from Pinus taeda, gi:515503, gi:1143308; contains Pfam
AMP-binding enzyme domain PF00501
Length = 566
Score = 28.7 bits (61), Expect = 3.2
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +1
Query: 520 IAVNECCTLVYTSGTVGPPKAVMLSHDN 603
I ++ ++Y+SGT G K V+L+H N
Sbjct: 201 IKQDDVAAIMYSSGTTGASKGVLLTHRN 228
>At1g21540.1 68414.m02694 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 9 AMPBP9 (AMPBP9) GI:20799726
Length = 550
Score = 28.3 bits (60), Expect = 4.2
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = +1
Query: 544 LVYTSGTVGPPKAVMLSH 597
L YTSGT PK V+LSH
Sbjct: 192 LNYTSGTTSSPKGVVLSH 209
>At1g21530.1 68414.m02693 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 10 AMPBP10 (AMPBP10) GI:20799728
Length = 547
Score = 28.3 bits (60), Expect = 4.2
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = +1
Query: 544 LVYTSGTVGPPKAVMLSH 597
L YTSGT PK V+LSH
Sbjct: 189 LNYTSGTTSSPKGVVLSH 206
>At3g28790.1 68416.m03593 expressed protein
Length = 608
Score = 27.9 bits (59), Expect = 5.5
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = +1
Query: 337 VQDKKQLDKILSVKHKLPLLKAIVQWEGPVDTSIPGI 447
V+ K+Q +++S+K K L+ A+V+W V T + +
Sbjct: 134 VEQKRQGSRMMSLKQKKELVVAMVKWTRMVITFVKSV 170
>At1g31812.1 68414.m03905 acyl-CoA binding protein / ACBP identical
to acyl-CoA-binding protein (ACBP) [Arabidopsis
thaliana] SWISS-PROT:P57752
Length = 92
Score = 27.5 bits (58), Expect = 7.3
Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 6/41 (14%)
Frame = +1
Query: 418 GPVDTSIPGIYS------WDQLLEIGAKEPDTQLNEILKSI 522
GPVDTS PG++S WD + K + +N+ + +
Sbjct: 39 GPVDTSRPGMFSMKERAKWDAWKAVEGKSSEEAMNDYITKV 79
>At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to adenosine monophosphate binding
protein 1 AMPBP1 (AMPBP1) GI:20799710
Length = 556
Score = 27.5 bits (58), Expect = 7.3
Identities = 39/172 (22%), Positives = 69/172 (40%), Gaps = 7/172 (4%)
Frame = +1
Query: 103 KITYKQYQDRVRIIAKAFLKLGLDRYHSVSILGFNSEQWFIADLXXXXXXXXXXXXXTTN 282
K T++Q +DR IA A +LG+ VS+L N +
Sbjct: 41 KYTWRQTRDRCVRIASALSQLGISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRH 100
Query: 283 SADACFHCLESSRANICA-----VQDKKQLDKILSVK-HKLPLLKAIVQWEGPVDTSIPG 444
+ L S + +Q + +ILS K K+P+L I + P+ S+
Sbjct: 101 DSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKGDKVPILVLIPE---PLTQSVSR 157
Query: 445 IYSWDQLLEIGAKEPDTQLN-EILKSIAVNECCTLVYTSGTVGPPKAVMLSH 597
++++E + + E+++ + ++ YTSGT PK V+ SH
Sbjct: 158 KKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSGTTSSPKGVVYSH 209
>At3g03570.1 68416.m00360 expressed protein similar to hypothetical
protein GB:CAB38918 [Arabidopsis thaliana]
Length = 607
Score = 27.1 bits (57), Expect = 9.6
Identities = 26/90 (28%), Positives = 42/90 (46%), Gaps = 7/90 (7%)
Frame = +1
Query: 325 NICAVQDKKQLDKILSVKHKLPLLKAIV--QWEGPVDTSI--PGIYSWDQLLEIGAKEPD 492
NIC + + +LS H PL+ A Q G + T + ++ L EI A + +
Sbjct: 433 NICGAEKGEPAALLLSPSHTPPLISADFSRQPSGSLFTIFLTSPVQAFCLLSEISASDME 492
Query: 493 TQL---NEILKSIAVNECCTLVYTSGTVGP 573
T + E L S ++NE + + TS T+ P
Sbjct: 493 TDIFTKAEKLLSSSMNEWASTLATSDTLHP 522
>At1g68600.1 68414.m07839 expressed protein contains Pfam profile
PF01027: Uncharacterized protein family UPF0005
Length = 537
Score = 27.1 bits (57), Expect = 9.6
Identities = 11/33 (33%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = -3
Query: 306 AVEACV-GRVGCIYSRRIPTSVDSCKIGDEPLF 211
++E CV G + C+ RIP+ + + + D+PL+
Sbjct: 236 SLEGCVNGYLQCVEYERIPSKILTYQASDDPLY 268
>At1g25480.1 68414.m03164 expressed protein contains Pfam profile
PF01027: Uncharacterized protein family UPF0005
Length = 548
Score = 27.1 bits (57), Expect = 9.6
Identities = 11/33 (33%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = -3
Query: 306 AVEACV-GRVGCIYSRRIPTSVDSCKIGDEPLF 211
++E CV G + C+ RIP+ + + + D+PL+
Sbjct: 247 SLEGCVNGYLQCVEYERIPSKILTYQASDDPLY 279
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,444,472
Number of Sequences: 28952
Number of extensions: 311225
Number of successful extensions: 936
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 893
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 922
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1206913392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -