BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0639
(389 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1A4.06c |||mitochondrial matrix protein import protein|Schiz... 27 1.0
SPAC1071.10c |pma1||P-type proton ATPase Pma1 |Schizosaccharomyc... 26 1.8
SPCC1020.01c |pma2|SPCC1393.01|P-type proton ATPase Pma2 |Schizo... 25 3.1
SPBC31A8.01c |cwl1|rtn1, SPBC651.13c|reticulon-like protein|Schi... 25 3.1
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 25 4.1
SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 5.5
SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 24 7.2
>SPBC1A4.06c |||mitochondrial matrix protein import
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 383
Score = 27.1 bits (57), Expect = 1.0
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1
Query: 187 MLKYITAKWLYRHSYS 234
+L+Y T +W+ RHSYS
Sbjct: 13 ILRYSTKRWMNRHSYS 28
>SPAC1071.10c |pma1||P-type proton ATPase Pma1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 919
Score = 26.2 bits (55), Expect = 1.8
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +2
Query: 275 VTQKQNRNAVMNHVFLRSLSFIEKTLHHNYLVRISR 382
+ Q QNR V N + F+E +L N+L+ ++R
Sbjct: 776 IAQGQNRGIVQNFGVQDEVLFLEISLTENWLIFVTR 811
>SPCC1020.01c |pma2|SPCC1393.01|P-type proton ATPase Pma2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1010
Score = 25.4 bits (53), Expect = 3.1
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +2
Query: 275 VTQKQNRNAVMNHVFLRSLSFIEKTLHHNYLVRISR 382
+ Q QNR V N + F++ +L N+L+ I+R
Sbjct: 864 IAQGQNRGIVQNFGVQDEVLFLQISLTENWLIFITR 899
>SPBC31A8.01c |cwl1|rtn1, SPBC651.13c|reticulon-like
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 308
Score = 25.4 bits (53), Expect = 3.1
Identities = 17/68 (25%), Positives = 28/68 (41%), Gaps = 1/68 (1%)
Frame = +1
Query: 136 YYNLKKSIAKTLDDYVIMLKYITAKWLYRHSYS*NVFSHLAGENILCDSKAESKCSNE-S 312
+ NL + +T ++ I L+ + V SH I CDSKA + N
Sbjct: 155 WINLPRLFFRTFRYVFLITSIIEFGGLFASNGKRGVLSHFRSSYITCDSKALDRIVNSIV 214
Query: 313 RFFTILII 336
F +++I
Sbjct: 215 DIFNVMLI 222
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 25.0 bits (52), Expect = 4.1
Identities = 9/18 (50%), Positives = 11/18 (61%)
Frame = +3
Query: 321 YDPYHLSRKRFTTIIWFV 374
Y PYH + K FT IW +
Sbjct: 696 YTPYHPNEKLFTAGIWTI 713
>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 24.6 bits (51), Expect = 5.5
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +1
Query: 148 KKSIAKTLDDYVIMLKYITAKWLY 219
K+S+ L+ I LKY + KWLY
Sbjct: 43 KESVLHFLELIFINLKYQSKKWLY 66
>SPAC16A10.08c |mug74|SPAC589.01c|sequence
orphan|Schizosaccharomyces pombe|chr 1|||Manual
Length = 285
Score = 24.2 bits (50), Expect = 7.2
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = +1
Query: 280 SKAESKCSNESRFFTILIIYRENASPQLFGS 372
S+AE C + L YREN SP L+GS
Sbjct: 139 SEAEKSCDFYGQ---PLHFYRENTSPCLYGS 166
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,563,985
Number of Sequences: 5004
Number of extensions: 29712
Number of successful extensions: 50
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 48
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 50
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 128029482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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