Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0635
(533 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g11910.1 68416.m01460 ubiquitin-specific protease, putative s... 78 4e-15
At5g06600.2 68418.m00746 ubiquitin-specific protease 12 (UBP12) ... 77 1e-14
At5g06600.1 68418.m00745 ubiquitin-specific protease 12 (UBP12) ... 77 1e-14
At1g32800.1 68414.m04043 PHD finger protein-related contains low... 31 0.48
At3g02090.2 68416.m00175 mitochondrial processing peptidase beta... 30 1.1
At3g02090.1 68416.m00174 mitochondrial processing peptidase beta... 30 1.1
At2g24650.1 68415.m02944 transcriptional factor B3 family protei... 29 1.9
At1g36510.1 68414.m04539 hypothetical protein 29 1.9
At3g06530.1 68416.m00757 BAP28-related similar to Protein BAP28 ... 27 5.9
At5g13290.1 68418.m01526 protein kinase family protein contains ... 27 7.9
>At3g11910.1 68416.m01460 ubiquitin-specific protease, putative strong
similarity to ubiquitin-specific protease 12 (UBP12)
[Arabidopsis thaliana] GI:11993471; contains Pfam
profiles PF00443: Ubiquitin carboxyl-terminal hydrolase,
PF00917: MATH domain
Length = 1115
Score = 77.8 bits (183), Expect = 4e-15
Identities = 50/145 (34%), Positives = 73/145 (50%), Gaps = 10/145 (6%)
Frame = +2
Query: 53 RIEEIPRDELNLQEDEMLVPCAHFYK-----QVYATFGIPFYARFKHNEPFEAVKDRLQK 217
R EEIP +E N+ ++ L+ HF K Q FG PF+ E E +K R+QK
Sbjct: 978 RAEEIPEEEKNIGPNDRLIHVYHFTKEAGQNQQVQNFGEPFFLVIHEGETLEEIKTRIQK 1037
Query: 218 KLDIPDKEWEKYNFAVVTNGRPNYISEGATVNINDFRLSSNTNLSHPDATARPWFGLEHI 397
KL +PD+++ K+ FA + GRP+Y+ + V N F+ + GLEHI
Sbjct: 1038 KLHVPDEDFAKWKFASFSMGRPDYLLD-TDVVYNRFQRRD------VYGAWEQYLGLEHI 1090
Query: 398 NKTPKRSRV-----NYLEKAIKIYN 457
+ PKR+ + EK +KIYN
Sbjct: 1091 DNAPKRAYAANQNRHAYEKPVKIYN 1115
>At5g06600.2 68418.m00746 ubiquitin-specific protease 12 (UBP12)
almost identical to ubiquitin-specific protease 12
GI:11993471 [Arabidopsis thaliana], one amino acid
difference
Length = 1115
Score = 76.6 bits (180), Expect = 1e-14
Identities = 49/145 (33%), Positives = 73/145 (50%), Gaps = 10/145 (6%)
Frame = +2
Query: 53 RIEEIPRDELNLQEDEMLVPCAHFYK-----QVYATFGIPFYARFKHNEPFEAVKDRLQK 217
R EEIP +E N+ ++ L+ HF K Q FG PF+ E E +K+R+QK
Sbjct: 978 RAEEIPEEEKNIGPNDRLILVYHFAKETGQNQQVQNFGEPFFLVIHEGETLEEIKNRIQK 1037
Query: 218 KLDIPDKEWEKYNFAVVTNGRPNYISEGATVNINDFRLSSNTNLSHPDATARPWFGLEHI 397
KL + D+++ K+ FA ++ GRP Y+ + V N F+ + GLEH
Sbjct: 1038 KLHVSDEDFAKWKFAFMSMGRPEYLQD-TDVVYNRFQRRD------VYGAFEQYLGLEHA 1090
Query: 398 NKTPKRSRV-----NYLEKAIKIYN 457
+ TPKR+ + EK +KIYN
Sbjct: 1091 DTTPKRAYAANQNRHAYEKPVKIYN 1115
>At5g06600.1 68418.m00745 ubiquitin-specific protease 12 (UBP12)
almost identical to ubiquitin-specific protease 12
GI:11993471 [Arabidopsis thaliana], one amino acid
difference
Length = 1116
Score = 76.6 bits (180), Expect = 1e-14
Identities = 49/145 (33%), Positives = 73/145 (50%), Gaps = 10/145 (6%)
Frame = +2
Query: 53 RIEEIPRDELNLQEDEMLVPCAHFYK-----QVYATFGIPFYARFKHNEPFEAVKDRLQK 217
R EEIP +E N+ ++ L+ HF K Q FG PF+ E E +K+R+QK
Sbjct: 979 RAEEIPEEEKNIGPNDRLILVYHFAKETGQNQQVQNFGEPFFLVIHEGETLEEIKNRIQK 1038
Query: 218 KLDIPDKEWEKYNFAVVTNGRPNYISEGATVNINDFRLSSNTNLSHPDATARPWFGLEHI 397
KL + D+++ K+ FA ++ GRP Y+ + V N F+ + GLEH
Sbjct: 1039 KLHVSDEDFAKWKFAFMSMGRPEYLQD-TDVVYNRFQRRD------VYGAFEQYLGLEHA 1091
Query: 398 NKTPKRSRV-----NYLEKAIKIYN 457
+ TPKR+ + EK +KIYN
Sbjct: 1092 DTTPKRAYAANQNRHAYEKPVKIYN 1116
>At1g32800.1 68414.m04043 PHD finger protein-related contains low
similarity to PHD-finger domain proteins
Length = 398
Score = 31.1 bits (67), Expect = 0.48
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = +2
Query: 155 PFYARFKHNEPFEAVKDRLQKKLDIPDKEW-EKYNFAVVTNGRP 283
P + E F A K+R++KK+++ DKE +K F T RP
Sbjct: 238 PMMTNKRRKELFGASKERMKKKVEVVDKEEDDKKGFVGKTGNRP 281
>At3g02090.2 68416.m00175 mitochondrial processing peptidase beta
subunit, putative similar to mitochondrial processing
peptidase beta subunit, mitochondrial precursor,
Beta-MPP [Human] SWISS-PROT:O75439
Length = 535
Score = 29.9 bits (64), Expect = 1.1
Identities = 20/67 (29%), Positives = 32/67 (47%), Gaps = 4/67 (5%)
Frame = +2
Query: 191 EAVKDRLQKKLDIPDKEWEKYNFAVVTNGRPNYISEGATVNI----NDFRLSSNTNLSHP 358
E +K+++ KKL+ PDK + KY N+I + N R+++ +NLS
Sbjct: 59 EIIKNKI-KKLENPDKRFLKYASPHPILASHNHILSAPETRVTTLPNGLRVATESNLSAK 117
Query: 359 DATARPW 379
AT W
Sbjct: 118 TATVGVW 124
>At3g02090.1 68416.m00174 mitochondrial processing peptidase beta
subunit, putative similar to mitochondrial processing
peptidase beta subunit, mitochondrial precursor,
Beta-MPP [Human] SWISS-PROT:O75439
Length = 531
Score = 29.9 bits (64), Expect = 1.1
Identities = 20/67 (29%), Positives = 32/67 (47%), Gaps = 4/67 (5%)
Frame = +2
Query: 191 EAVKDRLQKKLDIPDKEWEKYNFAVVTNGRPNYISEGATVNI----NDFRLSSNTNLSHP 358
E +K+++ KKL+ PDK + KY N+I + N R+++ +NLS
Sbjct: 59 EIIKNKI-KKLENPDKRFLKYASPHPILASHNHILSAPETRVTTLPNGLRVATESNLSAK 117
Query: 359 DATARPW 379
AT W
Sbjct: 118 TATVGVW 124
>At2g24650.1 68415.m02944 transcriptional factor B3 family protein
low similarity to reproductive meristem gene 1 from
[Brassica oleracea var. botrytis] GI:3170424,
[Arabidopsis thaliana] GI:13604227; contains Pfam
profile PF02362: B3 DNA binding domain
Length = 1440
Score = 29.1 bits (62), Expect = 1.9
Identities = 26/109 (23%), Positives = 48/109 (44%), Gaps = 7/109 (6%)
Frame = +2
Query: 116 AHFYKQVYATFGIPFYARFKHNEPF--EAVKDR--LQKKLDIPDKEWE-KYNFAVVTNGR 280
+ Y Q + T F+ E F E ++ R + KLD DK WE K + +T+G
Sbjct: 4 SRIYPQFFHTLVPSFHTHLMIPEDFFSEYIEGRSVAELKLDFSDKSWEVKLSDRRITDGW 63
Query: 281 PNYI--SEGATVNINDFRLSSNTNLSHPDATARPWFGLEHINKTPKRSR 421
++ ++ ++ FR N + H ++ +EH ++ PK +
Sbjct: 64 EEFVVANDFRIGDVVAFRYVGNL-VFHVSNLGPNYYEIEHNDELPKEKK 111
>At1g36510.1 68414.m04539 hypothetical protein
Length = 351
Score = 29.1 bits (62), Expect = 1.9
Identities = 15/28 (53%), Positives = 20/28 (71%), Gaps = 1/28 (3%)
Frame = -2
Query: 490 AGETRRAATGSVVYFDGLL-QVVDAGSL 410
+GE+R T S VY+D +L Q+VD GSL
Sbjct: 68 SGESRIIDTFSFVYYDDVLGQIVDVGSL 95
>At3g06530.1 68416.m00757 BAP28-related similar to Protein BAP28
(Swiss-Prot:Q9H583) [Homo sapiens]
Length = 1830
Score = 27.5 bits (58), Expect = 5.9
Identities = 11/40 (27%), Positives = 21/40 (52%)
Frame = +2
Query: 329 LSSNTNLSHPDATARPWFGLEHINKTPKRSRVNYLEKAIK 448
L N+++SHP ++ WF L H + + ++ L +K
Sbjct: 427 LDGNSDMSHPFVDSKLWFRLHHPRAAVRCAALSSLNGVLK 466
>At5g13290.1 68418.m01526 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 376
Score = 27.1 bits (57), Expect = 7.9
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +3
Query: 6 PSSHSTRSSYRRPDSTE*RRYLETN*IY 89
PSSH+ S Y P+S++ RY + + I+
Sbjct: 255 PSSHTAVSCYSAPESSQSNRYTDKSDIF 282
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,431,603
Number of Sequences: 28952
Number of extensions: 200224
Number of successful extensions: 614
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 592
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 607
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 984125600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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