Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0624
(523 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g21150.1 68418.m02526 PAZ domain-containing protein / piwi do... 68 4e-12
At2g27880.1 68415.m03380 argonaute protein, putative / AGO, puta... 58 4e-09
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 58 4e-09
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 58 4e-09
At2g27040.1 68415.m03249 PAZ domain-containing protein / piwi do... 58 5e-09
At1g69440.1 68414.m07979 PAZ domain-containing protein / piwi do... 57 8e-09
At1g31280.1 68414.m03828 PAZ domain-containing protein / piwi do... 56 1e-08
At1g31290.1 68414.m03829 PAZ domain-containing protein / piwi do... 56 1e-08
At5g43810.1 68418.m05357 pinhead protein (PINHEAD) / zwille prot... 49 2e-06
At5g21030.1 68418.m02501 PAZ domain-containing protein / piwi do... 48 4e-06
At2g32940.1 68415.m04038 PAZ domain-containing protein / piwi do... 45 4e-05
At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID... 31 0.62
At5g24090.1 68418.m02829 acidic endochitinase (CHIB1) identical ... 30 0.82
At5g55830.1 68418.m06957 lectin protein kinase, putative similar... 29 1.9
At4g14170.1 68417.m02188 pentatricopeptide (PPR) repeat-containi... 29 1.9
At2g37420.1 68415.m04589 kinesin motor protein-related 29 2.5
At3g21290.1 68416.m02690 dentin sialophosphoprotein-related cont... 28 3.3
At1g22010.1 68414.m02753 hypothetical protein 28 4.4
At4g23000.1 68417.m03318 calcineurin-like phosphoesterase family... 27 5.8
>At5g21150.1 68418.m02526 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|O04379 Argonaute
protein (AGO1) {Arabidopsis thaliana}; contains Pfam
profile: PF02170 PAZ (Piwi Argonaut and Zwille), PF02171
Piwi domain
Length = 896
Score = 67.7 bits (158), Expect = 4e-12
Identities = 50/152 (32%), Positives = 77/152 (50%), Gaps = 14/152 (9%)
Frame = +1
Query: 109 IVSKLMNTGRQMGMNVTQPKMACFN-----------IRINDLHKSMLHALEKQVNFLVVV 255
++ L+ GR+ G+NV P N +R+ ++ + + L K FL+ +
Sbjct: 495 LIRDLIKCGREKGINVEPPFKDVINENPQFRNAPATVRVENMFEQIKSKLPKPPLFLLCI 554
Query: 256 VSGR-GRDYYHKLKQIAELKVGILTQCIKEDTATRRMNPQTARNILLKVNSKLMGINQAL 432
++ R D Y K+ + +GI+TQCI A R+N Q N+LLK+N+KL G+N L
Sbjct: 555 LAERKNSDVYGPWKKKNLVDLGIVTQCI----APTRLNDQYLTNVLLKINAKLGGLNSLL 610
Query: 433 ENRSIPQCLKGGAV--MIVGADVTHPSPDQSN 522
P K V +IVG DV+H SP QS+
Sbjct: 611 AMERSPAMPKVTQVPTIIVGMDVSHGSPGQSD 642
>At2g27880.1 68415.m03380 argonaute protein, putative / AGO,
putative similar to SP|O04379 Argonaute protein (AGO1)
{Arabidopsis thaliana}; contains Pfam profiles PF02170:
PAZ domain, PF02171: Piwi domain
Length = 997
Score = 58.0 bits (134), Expect = 4e-09
Identities = 33/96 (34%), Positives = 54/96 (56%), Gaps = 3/96 (3%)
Frame = +1
Query: 244 LVVVVSGRGRDYYHKLKQIAELKVGILTQCIKEDTATRRMNPQTARNILLKVNSKLMGIN 423
L++V+ Y K+K+I E ++GI++QC + ++N Q N+ LK+N K G N
Sbjct: 638 LLIVILPDVTGSYGKIKRICETELGIVSQCC-QPRQVNKLNKQYMENVALKINVKTGGRN 696
Query: 424 QALEN---RSIPQCLKGGAVMIVGADVTHPSPDQSN 522
L + R+IP + +I+GADVTHP P + +
Sbjct: 697 TVLNDAIRRNIP-LITDRPTIIMGADVTHPQPGEDS 731
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 58.0 bits (134), Expect = 4e-09
Identities = 34/101 (33%), Positives = 55/101 (54%), Gaps = 3/101 (2%)
Frame = +1
Query: 229 KQVNFLVVVVSGRGRDYYHKLKQIAELKVGILTQCIKEDTATRRMNPQTARNILLKVNSK 408
K+++ L+V++ Y LK+I E ++GI++QC + M+ Q N+ LK+N K
Sbjct: 674 KEIDLLIVILPDNNGSLYGDLKRICETELGIVSQCCLTKHVFK-MSKQYMANVALKINVK 732
Query: 409 LMGINQALEN---RSIPQCLKGGAVMIVGADVTHPSPDQSN 522
+ G N L + R IP + +I GADVTHP P + +
Sbjct: 733 VGGRNTVLVDALSRRIP-LVSDRPTIIFGADVTHPHPGEDS 772
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 58.0 bits (134), Expect = 4e-09
Identities = 34/101 (33%), Positives = 55/101 (54%), Gaps = 3/101 (2%)
Frame = +1
Query: 229 KQVNFLVVVVSGRGRDYYHKLKQIAELKVGILTQCIKEDTATRRMNPQTARNILLKVNSK 408
K+++ L+V++ Y LK+I E ++GI++QC + M+ Q N+ LK+N K
Sbjct: 672 KEIDLLIVILPDNNGSLYGDLKRICETELGIVSQCCLTKHVFK-MSKQYMANVALKINVK 730
Query: 409 LMGINQALEN---RSIPQCLKGGAVMIVGADVTHPSPDQSN 522
+ G N L + R IP + +I GADVTHP P + +
Sbjct: 731 VGGRNTVLVDALSRRIP-LVSDRPTIIFGADVTHPHPGEDS 770
>At2g27040.1 68415.m03249 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|Q9QZ81
Eukaryotic translation initiation factor 2C 2 {Rattus
norvegicus}; contains Pfam profiles PF02171: Piwi
domain, PF02170: PAZ domain
Length = 924
Score = 57.6 bits (133), Expect = 5e-09
Identities = 38/116 (32%), Positives = 60/116 (51%), Gaps = 3/116 (2%)
Frame = +1
Query: 184 IRINDLHKSMLHALEKQVNFLVVVVSGR-GRDYYHKLKQIAELKVGILTQCIKEDTATRR 360
IR+ ++ K + L F++ V+ + D Y K+ + GI+TQC+ TR+
Sbjct: 558 IRVENMFKDIQSKLPGVPQFILCVLPDKKNSDLYGPWKKKNLTEFGIVTQCM---APTRQ 614
Query: 361 MNPQTARNILLKVNSKLMGINQALENRSIP--QCLKGGAVMIVGADVTHPSPDQSN 522
N Q N+LLK+N+KL G+N L P + +I+G DV+H SP QS+
Sbjct: 615 PNDQYLTNLLLKINAKLGGLNSMLSVERTPAFTVISKVPTIILGMDVSHGSPGQSD 670
>At1g69440.1 68414.m07979 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|Q9XGW1 PINHEAD
protein (ZWILLE protein) {Arabidopsis thaliana};
contains Pfam profiles PF02171: Piwi domain, PF02170:
PAZ domain
Length = 990
Score = 56.8 bits (131), Expect = 8e-09
Identities = 33/93 (35%), Positives = 53/93 (56%), Gaps = 4/93 (4%)
Frame = +1
Query: 244 LVVVVSGRGRDYYHKLKQIAELKVGILTQCIKEDTATRRMNPQTARNILLKVNSKLMGIN 423
L++ V + Y LK+I+E ++G++TQC T +++ Q N+ LK+N+K+ G
Sbjct: 649 LIICVMEKKHKGYGDLKRISETRIGVVTQCCLYPNIT-KLSSQFVSNLALKINAKIGGSM 707
Query: 424 QALEN---RSIPQCLK-GGAVMIVGADVTHPSP 510
L N IP+ L+ V+ +GADVTHP P
Sbjct: 708 TELYNSIPSHIPRLLRPDEPVIFMGADVTHPHP 740
>At1g31280.1 68414.m03828 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|O04379 Argonaute
protein (AGO1) {Arabidopsis thaliana}, SP|Q9XGW1 PINHEAD
protein (ZWILLE protein) {Arabidopsis thaliana};
contains Pfam profiles PF02171: Piwi domain, PF02170:
PAZ domain
Length = 1013
Score = 56.4 bits (130), Expect = 1e-08
Identities = 35/88 (39%), Positives = 48/88 (54%)
Frame = +1
Query: 244 LVVVVSGRGRDYYHKLKQIAELKVGILTQCIKEDTATRRMNPQTARNILLKVNSKLMGIN 423
LV+ R D Y LK IAE K+G++TQC AT+ Q N+ LK+N+K+ G N
Sbjct: 665 LVLCAMSRKDDGYKTLKWIAETKLGLVTQCFLTGPATKG-GDQYRANLALKMNAKVGGSN 723
Query: 424 QALENRSIPQCLKGGAVMIVGADVTHPS 507
L + + K VM +GADV HP+
Sbjct: 724 VELMD-TFSFFKKEDEVMFIGADVNHPA 750
>At1g31290.1 68414.m03829 PAZ domain-containing protein / piwi
domain-containing protein contains Pfam profiles PF02170:
PAZ domain, PF02171: Piwi domain
Length = 1194
Score = 56.0 bits (129), Expect = 1e-08
Identities = 33/90 (36%), Positives = 48/90 (53%), Gaps = 2/90 (2%)
Frame = +1
Query: 244 LVVVVSGRGRDYYHKLKQIAELKVGILTQCIKEDTATR--RMNPQTARNILLKVNSKLMG 417
LV+ D Y LK IAE K+G++TQC +A + ++ Q N+ LK+N+K+ G
Sbjct: 841 LVLCAMTGKHDGYKTLKWIAETKLGLVTQCFLTISAIKGETVSDQYLANLALKINAKVGG 900
Query: 418 INQALENRSIPQCLKGGAVMIVGADVTHPS 507
N L + K VM +GADV HP+
Sbjct: 901 TNVELVDNIFSFFKKEDKVMFIGADVNHPA 930
>At5g43810.1 68418.m05357 pinhead protein (PINHEAD) / zwille protein
(ZWILLE) identical to SP|Q9XGW1 PINHEAD protein (ZWILLE
protein) {Arabidopsis thaliana}; contains Pfam profiles
PF02171: Piwi domain, PF02170: PAZ domain
Length = 988
Score = 49.2 bits (112), Expect = 2e-06
Identities = 32/104 (30%), Positives = 52/104 (50%), Gaps = 3/104 (2%)
Frame = +1
Query: 202 HKSMLHALEKQVNFLVVVVSGRGRDYYHKLKQIAELKVGILTQCIKEDTATRRMNPQTAR 381
H SM K++ L+ ++ Y LK+I E ++G+++QC + ++ Q
Sbjct: 612 HTSMNKTKGKELELLLAILPDNNGSLYGDLKRICETELGLISQCCLTKHVFK-ISKQYLA 670
Query: 382 NILLKVNSKLMGINQALENR---SIPQCLKGGAVMIVGADVTHP 504
N+ LK+N K+ G N L + IP + +I GADVTHP
Sbjct: 671 NVSLKINVKMGGRNTVLVDAISCRIP-LVSDIPTIIFGADVTHP 713
>At5g21030.1 68418.m02501 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|O04379 Argonaute
protein (AGO1) {Arabidopsis thaliana}, SP|Q9XGW1 PINHEAD
protein (ZWILLE protein) {Arabidopsis thaliana};
contains Pfam profiles PF02171: Piwi domain, PF02170:
PAZ domain
Length = 850
Score = 48.0 bits (109), Expect = 4e-06
Identities = 41/155 (26%), Positives = 74/155 (47%), Gaps = 15/155 (9%)
Frame = +1
Query: 103 EDIVSKLMNTGRQMGMNVTQP-----------KMACFNIRINDLHKSMLHAL-EKQVNFL 246
+ I+ L G+ G+NV P K A ++R++ + + + L E FL
Sbjct: 470 QKIIDDLTRCGKMKGINVDSPYHVVFEENPQFKDATGSVRVDKMFQHLQSILGEVPPKFL 529
Query: 247 VVVVSGRGRDYYHKLKQIAELKVGILTQCIKEDTATRRMNPQTARNILLKVNSKLMGINQ 426
+ ++ + D Y K + +CI + +N Q N+LLK+N+KL G+N
Sbjct: 530 LCILEKKNSDVYEKSCSMWNC------ECI---VPPQNLNDQYLTNLLLKINAKLGGLNS 580
Query: 427 ALE---NRSIPQCLKGGAVMIVGADVTHPSPDQSN 522
L+ + ++P ++ + I+G DV+H SP QS+
Sbjct: 581 VLDMELSGTMPLVMRVPTI-IIGMDVSHGSPGQSD 614
>At2g32940.1 68415.m04038 PAZ domain-containing protein / piwi
domain-containing protein similar to SP|O04379 Argonaute
protein (AGO1) {Arabidopsis thaliana}, SP|Q9XGW1 PINHEAD
protein (ZWILLE protein) {Arabidopsis thaliana};
contains Pfam profiles PF02171: Piwi domain, PF02170:
PAZ domain
Length = 878
Score = 44.8 bits (101), Expect = 4e-05
Identities = 32/96 (33%), Positives = 51/96 (53%), Gaps = 3/96 (3%)
Frame = +1
Query: 244 LVVVVSGRGRDYYHKLKQIAELKVGILTQCIKEDTATRRMNPQTARNILLKVNSKLMGIN 423
L ++ + D Y K+I + GI TQCI +++ Q N+LLK+NSKL GIN
Sbjct: 543 LCILPERKTSDIYGPWKKICLTEEGIHTQCI----CPIKISDQYLTNVLLKINSKLGGIN 598
Query: 424 QAL---ENRSIPQCLKGGAVMIVGADVTHPSPDQSN 522
L + +IP + +I+G DV+H P +++
Sbjct: 599 SLLGIEYSYNIP-LINKIPTLILGMDVSHGPPGRAD 633
>At1g67120.1 68414.m07636 midasin-related similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q12019)
[Saccharomyces cerevisiae]; similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo
sapiens]; contains Prosite PS00017: ATP/GTP-binding site
motif A (P-loop)
Length = 5336
Score = 30.7 bits (66), Expect = 0.62
Identities = 20/88 (22%), Positives = 41/88 (46%), Gaps = 3/88 (3%)
Frame = +1
Query: 100 YEDIVSKLMNTGRQMGMNVTQPKMACFNIRINDLHKSMLHALEKQVNFLVVV---VSGRG 270
Y+++ L++ R + + F KS+ HA+EK+ N + + +S G
Sbjct: 3074 YQEMPGSLLSIARSLFQQIIYTHQKSFESETFVAIKSVFHAIEKKQNKMDGIQNLISLIG 3133
Query: 271 RDYYHKLKQIAELKVGILTQCIKEDTAT 354
++KLK + VG L + + D+++
Sbjct: 3134 SSSHNKLKSVTHSFVGPLAKRLYSDSSS 3161
>At5g24090.1 68418.m02829 acidic endochitinase (CHIB1) identical to
SP|P19172 Acidic endochitinase precursor (EC 3.2.1.14)
{Arabidopsis thaliana}
Length = 302
Score = 30.3 bits (65), Expect = 0.82
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = -1
Query: 166 LVGSRSCPFVDRYSLASTRCLHNTSTWIQFYGN 68
L G+ CPF DR ++ WIQFY N
Sbjct: 182 LTGAPQCPFPDRLMGSALNTKRFDYVWIQFYNN 214
>At5g55830.1 68418.m06957 lectin protein kinase, putative similar to
receptor lectin kinase 3 [Arabidopsis thaliana]
gi|4100060|gb|AAD00733; contains protein kinase domain,
Pfam:PF00069; contains legume lectins alpha and beta
domains, Pfam:PF00138 and Pfam:PF00139
Length = 681
Score = 29.1 bits (62), Expect = 1.9
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -3
Query: 197 SFIRMLKQAIFGWVTFMPICRPVFISFDTMS 105
S R L F W+T + + +P+F+S D M+
Sbjct: 2 SLSRKLLVIFFTWITALSMSKPIFVSSDNMN 32
>At4g14170.1 68417.m02188 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 458
Score = 29.1 bits (62), Expect = 1.9
Identities = 27/87 (31%), Positives = 40/87 (45%), Gaps = 4/87 (4%)
Frame = -2
Query: 477 HHSPTL*ALRNGTILQCLIYSHKLRIHL**NV---PSCLWVHSARGGIFFDTLRQYTHFE 307
HHSP LR+ +++H LR L NV + +S +F +L + H
Sbjct: 23 HHSPNAKHLRH-------LHAHLLRTFLYSNVVLSSKLVLAYSKLNHLFPTSLSVFWHMP 75
Query: 306 FGNLFQLVIIIAAPSRNDH-DQEIDLF 229
+ N+F III SR+ + IDLF
Sbjct: 76 YRNIFSWNIIIGEFSRSGFASKSIDLF 102
>At2g37420.1 68415.m04589 kinesin motor protein-related
Length = 1039
Score = 28.7 bits (61), Expect = 2.5
Identities = 22/99 (22%), Positives = 39/99 (39%)
Frame = +1
Query: 118 KLMNTGRQMGMNVTQPKMACFNIRINDLHKSMLHALEKQVNFLVVVVSGRGRDYYHKLKQ 297
K +NT Q +NVT MA N ++ D S +++ + D + ++
Sbjct: 873 KNLNTKYQQELNVTMEDMAKENEKVQDEFTSTFSSMDANFVSRTNELHAAVNDSLMQDRE 932
Query: 298 IAELKVGILTQCIKEDTATRRMNPQTARNILLKVNSKLM 414
E I+ C+ + T + + Q NI K L+
Sbjct: 933 NKETTEAIVETCMNQVTLLQENHGQAVSNIRNKAEQSLI 971
>At3g21290.1 68416.m02690 dentin sialophosphoprotein-related contains
weak similarity to Dentin sialophosphoprotein precursor
(Swiss-Prot:Q9NZW4) [Homo sapiens]
Length = 1192
Score = 28.3 bits (60), Expect = 3.3
Identities = 22/69 (31%), Positives = 39/69 (56%), Gaps = 2/69 (2%)
Frame = +1
Query: 223 LEKQVNFLVVVVSGRGRDYYHKLKQIAELKVGILTQCIKEDTATRRMNPQTA-RNILLKV 399
L+ Q +L + G G KQ++EL++G L + + EDTA + + +T+ R LK
Sbjct: 863 LDSQRTYLAKLPKGNGPVLQ---KQVSELELGELPEPLGEDTALKPIEEKTSFRQSNLKP 919
Query: 400 N-SKLMGIN 423
+ S+ +GI+
Sbjct: 920 STSEKLGID 928
>At1g22010.1 68414.m02753 hypothetical protein
Length = 163
Score = 27.9 bits (59), Expect = 4.4
Identities = 14/49 (28%), Positives = 25/49 (51%)
Frame = +1
Query: 280 YHKLKQIAELKVGILTQCIKEDTATRRMNPQTARNILLKVNSKLMGINQ 426
Y K I+ +V IL QC+ + T + +++ L V+ +MGI +
Sbjct: 107 YITFKFISRTRVRILEQCVLSEMTTTSRIFEDEKDMWLPVDESVMGIEE 155
>At4g23000.1 68417.m03318 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 932
Score = 27.5 bits (58), Expect = 5.8
Identities = 12/31 (38%), Positives = 21/31 (67%)
Frame = +1
Query: 418 INQALENRSIPQCLKGGAVMIVGADVTHPSP 510
IN L+N SI L+ G ++++G D+ +P+P
Sbjct: 438 INVPLDNDSIS--LERGNILLIGGDLAYPNP 466
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,133,873
Number of Sequences: 28952
Number of extensions: 288249
Number of successful extensions: 723
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 693
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 713
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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