Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0604
(416 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putati... 116 8e-27
At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putati... 113 5e-26
At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putati... 109 1e-24
At2g11270.1 68415.m01208 citrate synthase-related contains simil... 44 4e-05
At2g21720.1 68415.m02584 hypothetical protein contains Pfam prof... 29 1.3
At2g20290.1 68415.m02370 myosin, putative similar to myosin (GI:... 29 1.7
At5g41770.1 68418.m05086 crooked neck protein, putative / cell c... 28 2.2
At4g16442.1 68417.m02489 integral membrane family protein contai... 28 2.2
At3g15750.1 68416.m01995 expressed protein 28 2.2
At5g17490.1 68418.m02052 gibberellin response modulator, putativ... 28 2.9
At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical... 27 3.8
At1g75870.1 68414.m08812 hypothetical protein 27 3.8
At3g61570.1 68416.m06896 intracellular protein transport protein... 27 5.1
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 27 5.1
At1g76950.1 68414.m08958 zinc finger protein (PRAF1) / regulator... 27 5.1
At1g15210.1 68414.m01818 ABC transporter family protein Similar ... 27 5.1
At5g55520.1 68418.m06915 expressed protein weak similarity to ph... 27 6.7
At4g04530.1 68417.m00660 Ulp1 protease family protein (snoR29) c... 27 6.7
At1g34570.1 68414.m04296 expressed protein 27 6.7
At1g23050.1 68414.m02880 hydroxyproline-rich glycoprotein family... 27 6.7
At1g09870.1 68414.m01111 histidine acid phosphatase family prote... 27 6.7
At4g04950.1 68417.m00719 thioredoxin family protein similar to P... 26 8.9
At2g35760.1 68415.m04388 integral membrane family protein contai... 26 8.9
At1g65630.1 68414.m07444 DegP protease, putative contains simila... 26 8.9
>At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 474
Score = 116 bits (278), Expect = 8e-27
Identities = 53/113 (46%), Positives = 80/113 (70%)
Frame = +2
Query: 77 REVALFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHG 256
R V+ F SR+ Q + + ++ S+ +LKS LQE IP++Q+++++ + +HG
Sbjct: 5 RSVSAFTRLRSRVQGQQSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLKSEHG 64
Query: 257 STKVGEVTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAK 415
++G +TVDM+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+
Sbjct: 65 KVQLGNITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQ 117
>At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 473
Score = 113 bits (272), Expect = 5e-26
Identities = 53/113 (46%), Positives = 81/113 (71%)
Frame = +2
Query: 77 REVALFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHG 256
R V+ F SR+ + Q + + ++ S+ +LKS LQE IP++Q+++++ + +HG
Sbjct: 5 RSVSAFTRLRSRVGQ-QSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLKSEHG 63
Query: 257 STKVGEVTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAK 415
++G +TVDM+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+
Sbjct: 64 KVQLGNITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQ 116
>At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putative
strong similarity to SP|Q43175 Citrate synthase,
mitochondrial precursor {Solanum tuberosum}; contains
Pfam profile PF00285: Citrate synthase
Length = 433
Score = 109 bits (261), Expect = 1e-24
Identities = 46/78 (58%), Positives = 65/78 (83%)
Frame = +2
Query: 182 NLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGLVWETSVLDADEGI 361
+LKS +QE IP++Q+++++ + + G VG +TVDM+ GGMRG+ GL+WETS+LDADEGI
Sbjct: 5 DLKSQMQEIIPEQQDRLKKLKSEQGKVPVGNITVDMVLGGMRGMTGLLWETSLLDADEGI 64
Query: 362 RFRGLSIPECQQQLPKAK 415
RFRG+SIPECQ+ LP A+
Sbjct: 65 RFRGMSIPECQKILPSAE 82
>At2g11270.1 68415.m01208 citrate synthase-related contains
similarity to Swiss-Prot:P20115 citrate synthase,
mitochondrial precursor [Arabidopsis thaliana]
Length = 83
Score = 44.0 bits (99), Expect = 4e-05
Identities = 17/34 (50%), Positives = 25/34 (73%)
Frame = +2
Query: 221 QEKIREFRKKHGSTKVGEVTVDMMYGGMRGIKGL 322
Q++ ++ + KHG VG +TVDM+ GGMRG+ GL
Sbjct: 43 QDRSKKLKLKHGKVPVGNITVDMVLGGMRGMTGL 76
>At2g21720.1 68415.m02584 hypothetical protein contains Pfam
profile: PF04842 plant protein of unknown function
(DUF639)
Length = 703
Score = 29.1 bits (62), Expect = 1.3
Identities = 16/35 (45%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = -3
Query: 135 HAFCNSTSLEDVILNRATSLKA-AERKLPHKSRQL 34
H C + E VIL+ +TSLK A+RK KS++L
Sbjct: 13 HVACGEVASERVILSTSTSLKPNAKRKKNSKSKKL 47
>At2g20290.1 68415.m02370 myosin, putative similar to myosin
(GI:499047) [Arabidopsis thaliana]
Length = 1493
Score = 28.7 bits (61), Expect = 1.7
Identities = 21/62 (33%), Positives = 36/62 (58%), Gaps = 4/62 (6%)
Frame = +2
Query: 74 LREVALFRITSSRLVELQKACPTA-TVLLRGLSAEQTNLKSI---LQEKIPKEQEKIREF 241
L+EV + T++ +V + K P TVL+ L++E LKS+ L+ KI + ++K E
Sbjct: 950 LKEVEAAKKTAA-IVPVVKEVPVVDTVLMEKLTSENEKLKSLVTSLELKIDETEKKFEET 1008
Query: 242 RK 247
+K
Sbjct: 1009 KK 1010
>At5g41770.1 68418.m05086 crooked neck protein, putative / cell
cycle protein, putative similar to Swiss-Prot:P17886
crooked neck protein [Drosophila melanogaster]
Length = 705
Score = 28.3 bits (60), Expect = 2.2
Identities = 13/35 (37%), Positives = 23/35 (65%)
Frame = +2
Query: 191 SILQEKIPKEQEKIREFRKKHGSTKVGEVTVDMMY 295
SI+Q K+PK+ +K + ++ GST+ E +D +Y
Sbjct: 643 SIVQSKLPKKLKKRKAITREDGSTEYEEY-IDYLY 676
>At4g16442.1 68417.m02489 integral membrane family protein contains
TIGRFAM TIGR01569 : plant integral membrane protein
TIGR01569; contains Pfam PF04535 : Domain of unknown
function (DUF588)
Length = 182
Score = 28.3 bits (60), Expect = 2.2
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +3
Query: 279 QLI*CTVACVESKVWFGKPLCWMPMKESVSVVYPSLSA 392
Q + C V ++ KV F KPL W ++ Y +++A
Sbjct: 71 QSVRCVVGTMKGKVLFSKPLAWAFFSGDQAMAYLNVAA 108
>At3g15750.1 68416.m01995 expressed protein
Length = 186
Score = 28.3 bits (60), Expect = 2.2
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = +2
Query: 152 LLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDM 289
L+RG+S+ L L+EK+ +QE +F+K H S V M
Sbjct: 79 LVRGVSSALAFLPDELREKLIDKQETREKFQKLHSSVHALSTEVAM 124
>At5g17490.1 68418.m02052 gibberellin response modulator, putative /
gibberellin-responsive modulator, putative putative
member of the VHIID domain transcription factor family
RGAL - Arabidopsis thaliana, EMBL:AJ224957
Length = 523
Score = 27.9 bits (59), Expect = 2.9
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = +2
Query: 50 CGSLRSAALREVALFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSI 196
C S+ S + R V L T RLV+ AC A V L LS +K +
Sbjct: 136 CDSVTSESTRSVVLIEETGVRLVQALVACAEA-VQLENLSLADALVKRV 183
>At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical to
EIN2 [Arabidopsis thaliana] gi|5231113|gb|AAD41076;
member of the natural resistance-associated macrophage
protein (NRAMP) metal transporter family, PMID:11500563;
metal transport capacity has not been shown,
PMID:11500563, PMID:1038174
Length = 1294
Score = 27.5 bits (58), Expect = 3.8
Identities = 18/68 (26%), Positives = 29/68 (42%)
Frame = -1
Query: 251 ASFGIRGSSLVPWESFPGEYFLNSSVQRLDLEVELSQSDMLSAILRVLKM*S*IEPPLLK 72
A G+ G+S+VP + YF S D++ D L AI V S + L+
Sbjct: 198 ALMGLLGASIVPHNFYIHSYFAGESTSSSDVDKSSLCQDHLFAIFGVFSGLSLVNYVLMN 257
Query: 71 PPSANYHT 48
+ +H+
Sbjct: 258 AAANVFHS 265
>At1g75870.1 68414.m08812 hypothetical protein
Length = 165
Score = 27.5 bits (58), Expect = 3.8
Identities = 18/78 (23%), Positives = 36/78 (46%), Gaps = 3/78 (3%)
Frame = +2
Query: 41 RDLCGSLRSAALREVALFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKE 220
R+L G LRS R + R+ + +++K C + R AE + + EK
Sbjct: 52 RELYGQLRSLEERAIFCVRVKDKVIEDMKKQCGEMEIRTRKALAEAEFWRKMTNEKTDLC 111
Query: 221 QE---KIREFRKKHGSTK 265
++ ++ E +K+ +T+
Sbjct: 112 RDLAGRLIEMKKRERATR 129
>At3g61570.1 68416.m06896 intracellular protein transport protein
USO1-related contains weak similarity to intracellular
protein transport protein USO1 (Swiss-Prot:P25386)
[Saccharomyces cerevisiae]
Length = 712
Score = 27.1 bits (57), Expect = 5.1
Identities = 10/26 (38%), Positives = 20/26 (76%)
Frame = -1
Query: 212 ESFPGEYFLNSSVQRLDLEVELSQSD 135
ESFPG+ + S+QRL+++++ +Q +
Sbjct: 336 ESFPGKEEMEQSLQRLEMDLKETQRE 361
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 27.1 bits (57), Expect = 5.1
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -3
Query: 378 DRPRKRIPSSASSTEVSQTRPLIPRMP 298
DR R+R PSS+ S S++ P++ R P
Sbjct: 604 DRRRRRSPSSSRSPSRSRSPPVLHRSP 630
>At1g76950.1 68414.m08958 zinc finger protein (PRAF1) / regulator of
chromosome condensation (RCC1) family protein identical
to zinc finger protein PRAF1 [Arabidopsis thaliana]
gi|15811367|gb|AAL08940.
Length = 1103
Score = 27.1 bits (57), Expect = 5.1
Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 3/50 (6%)
Frame = -3
Query: 393 WHSG---MDRPRKRIPSSASSTEVSQTRPLIPRMPPYIISTVTSPTLVEP 253
W G +D R+ SS SS+ S +R P+ I +TSP EP
Sbjct: 136 WSGGGLSVDASRELTSSSPSSSSASASRGHSSPGTPFNIDPITSPKSAEP 185
>At1g15210.1 68414.m01818 ABC transporter family protein Similar to
gb|Z70524 GI:1514643 PDR5-like ABC transporter from
Spirodela polyrrhiza and is a member of the PF|00005 ABC
transporter family. ESTs gb|N97039 and gb|T43169 come
from this gene
Length = 1442
Score = 27.1 bits (57), Expect = 5.1
Identities = 15/37 (40%), Positives = 19/37 (51%)
Frame = -3
Query: 192 LFKFVCSALRPRSRTVAVGHAFCNSTSLEDVILNRAT 82
L K V SA RP T +G + T+L DV+ R T
Sbjct: 867 LLKGVTSAFRPGVLTALMGVSGAGKTTLMDVLAGRKT 903
>At5g55520.1 68418.m06915 expressed protein weak similarity to
phragmoplast-associated kinesin-related protein 1
[Arabidopsis thaliana] GI:8745333; expression supported
by MPSS
Length = 802
Score = 26.6 bits (56), Expect = 6.7
Identities = 25/81 (30%), Positives = 35/81 (43%), Gaps = 1/81 (1%)
Frame = +2
Query: 161 GLSAEQTNLKSILQEKIPKEQEKIREFRKK-HGSTKVGEVTVDMMYGGMRGIKGLVWETS 337
G+S + IL+E E KIR FRK STK ++ G + + T
Sbjct: 258 GISISLPHQSRILEEPPLSESPKIRNFRKSVAASTKFQASPRNVTESSSTGNRKPLSPTD 317
Query: 338 VLDADEGIRFRGLSIPECQQQ 400
L A RGL+I +C Q+
Sbjct: 318 SLAAS---LQRGLNIIDCHQR 335
>At4g04530.1 68417.m00660 Ulp1 protease family protein (snoR29)
contains Pfam profile PF02902: Ulp1 protease family,
C-terminal catalytic domain; snoR29 gene for small
nucleolar RNA GI:15706258
Length = 917
Score = 26.6 bits (56), Expect = 6.7
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = -3
Query: 354 SSASSTEVSQTRPLIPRMPPYIISTVTSPTLVEPCFFRNSRI 229
S+ S+ E S P+IPR+P + + C+ N RI
Sbjct: 35 SAKSTAEQSSDPPIIPRIPEFNRERLPKRLYATDCYPLNGRI 76
>At1g34570.1 68414.m04296 expressed protein
Length = 208
Score = 26.6 bits (56), Expect = 6.7
Identities = 15/46 (32%), Positives = 23/46 (50%)
Frame = +2
Query: 152 LLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGSTKVGEVTVDM 289
++RG+S+ L +EK+ EQE +F+K H S V M
Sbjct: 101 IVRGVSSALAFLPVEFREKLIDEQETREKFQKLHSSVHALSTEVAM 146
>At1g23050.1 68414.m02880 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 161
Score = 26.6 bits (56), Expect = 6.7
Identities = 11/24 (45%), Positives = 12/24 (50%)
Frame = -1
Query: 89 EPPLLKPPSANYHTNHDNLPAIDP 18
+PP PP NY T NLP P
Sbjct: 53 QPPPYPPPGVNYPTPAGNLPNYPP 76
>At1g09870.1 68414.m01111 histidine acid phosphatase family protein
contains Pfam profile PF00328: Histidine acid
phosphatase; similar to multiple inositol polyphosphate
phosphatase (GI:4105496)[Mus musculus]; EST gb|R64758
comes from this gene
Length = 487
Score = 26.6 bits (56), Expect = 6.7
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -3
Query: 111 LEDVILNRATSLKAAERKLPHKSRQLARDR 22
LEDV+ + ++KA E KLP S + AR R
Sbjct: 311 LEDVLHSMEEAIKAREEKLPPGSYEKARLR 340
>At4g04950.1 68417.m00719 thioredoxin family protein similar to
PKCq-interacting protein PICOT from [Mus musculus]
GI:6840949, [Rattus norvegicus] GI:6840951; contains
Pfam profile PF00085: Thioredoxin
Length = 488
Score = 26.2 bits (55), Expect = 8.9
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = -1
Query: 215 WESFPGEYFLNSSVQRLDLEVELSQSDMLSAIL 117
W +FP Y+ + D+ +ELS+S L A L
Sbjct: 454 WPTFPQLYYKGELIGGCDIIMELSESGDLKATL 486
>At2g35760.1 68415.m04388 integral membrane family protein contains
TIGRFAM TIGR01569 : plant integral membrane protein
TIGR01569; contains Pfam PF04535 : Domain of unknown
function (DUF588)
Length = 201
Score = 26.2 bits (55), Expect = 8.9
Identities = 11/33 (33%), Positives = 16/33 (48%)
Frame = +3
Query: 279 QLI*CTVACVESKVWFGKPLCWMPMKESVSVVY 377
Q + C V ++ +V F KPL W +V Y
Sbjct: 90 QAVRCVVGLMKGRVLFSKPLAWAIFFGDQAVAY 122
>At1g65630.1 68414.m07444 DegP protease, putative contains
similarity to DegP2 protease GI:13172275 from
[Arabidopsis thaliana]
Length = 559
Score = 26.2 bits (55), Expect = 8.9
Identities = 19/46 (41%), Positives = 25/46 (54%), Gaps = 3/46 (6%)
Frame = +2
Query: 95 RITSSRLVELQKACPTA--TVLLRGLSAE-QTNLKSILQEKIPKEQ 223
RIT LV ++K C TA VL G E ++LKS+ +PK Q
Sbjct: 366 RITFKHLVSMKKPCETALLKVLREGKEYEFNSSLKSV-PPLVPKRQ 410
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,405,177
Number of Sequences: 28952
Number of extensions: 187282
Number of successful extensions: 745
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 745
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 635399168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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