Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0591
(527 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) / phosph... 37 0.010
At4g34580.1 68417.m04913 SEC14 cytosolic factor, putative / phos... 33 0.090
At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /... 32 0.21
At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein /... 32 0.27
At2g21540.1 68415.m02563 SEC14 cytosolic factor, putative / phos... 31 0.63
At4g39180.1 68417.m05548 SEC14 cytosolic factor, putative / phos... 30 0.84
At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /... 30 0.84
At5g47510.1 68418.m05866 SEC14 cytosolic factor family protein /... 30 1.1
At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative / poly... 29 1.9
At3g42060.1 68416.m04315 myosin heavy chain-related 29 1.9
At5g44550.1 68418.m05457 integral membrane family protein simila... 29 2.6
At4g08690.1 68417.m01432 SEC14 cytosolic factor family protein /... 29 2.6
At2g16380.1 68415.m01874 SEC14 cytosolic factor family protein /... 29 2.6
At1g01630.1 68414.m00080 SEC14 cytosolic factor, putative / phos... 28 3.4
At4g31540.1 68417.m04478 exocyst subunit EXO70 family protein (E... 28 4.5
At1g22180.2 68414.m02774 SEC14 cytosolic factor family protein /... 28 4.5
At1g56020.1 68414.m06431 expressed protein 27 5.9
At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /... 27 7.8
>At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) /
phosphoglyceride transfer protein similar to
polyphosphoinositide binding protein SEC14 homolog Ssh1p
(GB:AAB94598) [Glycine max]; identified in Eur J Biochem
1998 Dec 1;258(2):402-10 as AtSEC14, characterized by
functional complementation in S. cerevisiae.
Length = 325
Score = 36.7 bits (81), Expect = 0.010
Identities = 29/92 (31%), Positives = 49/92 (53%), Gaps = 5/92 (5%)
Frame = +2
Query: 161 EDPNTRLQAIYE-LRDMIYERGECTPHRMDDEFLIRFLRARNFIPQKAHRLLVNYYQFRA 337
E+ +L+A+ E + D + E E L+RFL+AR+ QKAH++L+ ++R
Sbjct: 6 EEAVKQLRALMEDVDDSLRESYRNIHQGYPTENLLRFLKARDGNVQKAHKMLLECLEWRT 65
Query: 338 EN---PDLFENIHPMDLH-AIGDADIMTVPPY 421
+N L + I P+DL+ I D ++ V Y
Sbjct: 66 QNEIDKILTKPIVPVDLYRGIRDTQLVGVSGY 97
>At4g34580.1 68417.m04913 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein III
(GI:14486705) [Lotus japonicus]; similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; similar SEC14 protein,
Saccharomyces cerevisiae, PIR2:A30106; contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus
Length = 560
Score = 33.5 bits (73), Expect = 0.090
Identities = 31/102 (30%), Positives = 46/102 (45%), Gaps = 4/102 (3%)
Frame = +2
Query: 179 LQAIYELRDMIYERGECTPHRMDD-EFLIRFLRARNFIPQKAHRLLVNYYQFRAE-NPDL 352
LQA+ R + E P ++DD ++RFLRAR F +KA ++ + Q+R + D
Sbjct: 63 LQALDAFRQALI-LDELLPSKLDDLHMMLRFLRARKFDIEKAKQMWSDMIQWRKDFGADT 121
Query: 353 FENIHPMDLHAIGDADIMTVPPYRGPD--GRRLIIYKFGNWD 472
I D I + Y G D GR + I + G D
Sbjct: 122 I--IEDFDFEEIDEVMKHYPQGYHGVDKEGRPVYIERLGQID 161
>At1g22530.1 68414.m02814 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to SEC14-like protein 2
(Alpha-tocopherol associated protein) (TAP) (bTAP)
(Fragment) (SP:P58875) {Bos taurus}
Length = 683
Score = 32.3 bits (70), Expect = 0.21
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +2
Query: 248 DEFLIRFLRARNFIPQKAHRLLVNYYQFRAEN 343
D L++FLRAR+F ++A +L N Q+R EN
Sbjct: 365 DVILLKFLRARDFKVKEAFTMLKNTVQWRKEN 396
>At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
polyphosphoinositide binding protein Ssh1p (GI:2739044)
{Glycine max}; similar to polyphosphoinositide binding
protein Ssh2, Glycine max, gb:T05953; contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus
Length = 668
Score = 31.9 bits (69), Expect = 0.27
Identities = 14/31 (45%), Positives = 22/31 (70%)
Frame = +2
Query: 248 DEFLIRFLRARNFIPQKAHRLLVNYYQFRAE 340
D L++FLRAR+F PQ+A+ +L Q+R +
Sbjct: 339 DVVLLKFLRARDFKPQEAYSMLNKTLQWRID 369
>At2g21540.1 68415.m02563 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; contains Pfam PF00650
: CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO,
N-terminus
Length = 548
Score = 30.7 bits (66), Expect = 0.63
Identities = 19/55 (34%), Positives = 29/55 (52%), Gaps = 1/55 (1%)
Frame = +2
Query: 179 LQAIYELRDMIYERGECTPHRMDDE-FLIRFLRARNFIPQKAHRLLVNYYQFRAE 340
LQA+ R + E P + DD ++RFLRAR F +KA ++ + +R E
Sbjct: 70 LQAVDAFRQALI-LDELLPSKHDDHHMMLRFLRARKFDLEKAKQMWTDMIHWRKE 123
>At4g39180.1 68417.m05548 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative
phosphatidylinositol-phosphatidylcholine transfer
protein SEC14, Yarrowia lipolytica, PIR2:S43745;
contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam
PF03765 : CRAL/TRIO, N-terminus
Length = 554
Score = 30.3 bits (65), Expect = 0.84
Identities = 30/102 (29%), Positives = 44/102 (43%), Gaps = 4/102 (3%)
Frame = +2
Query: 179 LQAIYELRDMIYERGECTPHRMDDE-FLIRFLRARNFIPQKAHRLLVNYYQFRAE-NPDL 352
LQA+ R + E P + DD ++RFLRAR F +KA ++ + +R E D
Sbjct: 71 LQAVDAFRQALI-LDELLPSKHDDHHMMLRFLRARKFDLEKAKQMWSDMLNWRKEYGADT 129
Query: 353 FENIHPMDLHAIGDADIMTVPPYRGPD--GRRLIIYKFGNWD 472
+ D I + Y G D GR + I + G D
Sbjct: 130 I--MEDFDFKEIEEVVKYYPQGYHGVDKEGRPIYIERLGQVD 169
>At1g72150.1 68414.m08342 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
SEC14-like protein 2 (Alpha-tocopherol associated
protein) (TAP) (bTAP) (Fragment) (SP:P58875) {Bos
taurus}; similar to GI:807956 from [Saccharomyces
cerevisiae]; contains Pfam PF00650 : CRAL/TRIO domain;
contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 573
Score = 30.3 bits (65), Expect = 0.84
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = +2
Query: 248 DEFLIRFLRARNFIPQKAHRLLVNYYQFRAEN 343
D L +FLRAR+F ++A +L N Q+R EN
Sbjct: 256 DVILTKFLRARDFKVKEALTMLKNTVQWRKEN 287
>At5g47510.1 68418.m05866 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus], SEC14 cytosolic factor
(Phosphatidylinositol/phosphatidylcholine transfer
protein) (PI/PCTP) (SP:P24859) [Kluyveromyces lactis]
and to SEC14 cytosolic factor (SP:P53989) [Candida
glabrata]
Length = 376
Score = 29.9 bits (64), Expect = 1.1
Identities = 15/60 (25%), Positives = 29/60 (48%)
Frame = +2
Query: 161 EDPNTRLQAIYELRDMIYERGECTPHRMDDEFLIRFLRARNFIPQKAHRLLVNYYQFRAE 340
+ PN + + R+++ G D L RFL+ R+F +K+ +NY ++R +
Sbjct: 20 QSPNNE-EMVEAFRNLLLLHGHLPDKHGDHNTLRRFLKMRDFDLEKSKEAFLNYMKWRVD 78
>At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative /
polyphosphoinositide-binding protein, putative similar
to polyphosphoinositide binding protein SEC14 homolog
Ssh1p (GI:2739044) {Glycine max}
Length = 341
Score = 29.1 bits (62), Expect = 1.9
Identities = 19/54 (35%), Positives = 30/54 (55%), Gaps = 4/54 (7%)
Frame = +2
Query: 194 ELRDMIYERGECTPHRMDDEFLI----RFLRARNFIPQKAHRLLVNYYQFRAEN 343
EL D + E + T R+ +L RFL+AR++ KAH +LV ++R +N
Sbjct: 14 ELMDQVEEPLKKTYERVHQGYLRENLGRFLKARDWNVCKAHTMLVECLRWRVDN 67
>At3g42060.1 68416.m04315 myosin heavy chain-related
Length = 712
Score = 29.1 bits (62), Expect = 1.9
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = -2
Query: 511 CCFEQFFHANGLWVPITKFI 452
C FE +F GLW P+ +F+
Sbjct: 199 CLFESYFTEGGLWFPLPEFL 218
>At5g44550.1 68418.m05457 integral membrane family protein similar
to unknown protein (pir||T10581) This family of plant
proteins contains a domain that may have a catalytic
activity. It has a conserved arginine and aspartate that
could form an active site. These proteins are predicted
to contain 3 or 4 transmembrane helices; contains
TIGRFAM TIGR01569 : plant integral membrane protein
TIGR01569; contains Pfam PF04535 : Domain of unknown
function (DUF588)
Length = 197
Score = 28.7 bits (61), Expect = 2.6
Identities = 18/63 (28%), Positives = 30/63 (47%)
Frame = -2
Query: 241 PVRGTFTALINHVP*FVDGLKSSIGVLAAMLTSVVLDLWRGLAEVQVHAVIRSRHVGRLN 62
P++ TFTA +H P FV + ++ V L + L ++ G E ++ + LN
Sbjct: 55 PIQATFTAKFDHTPAFVFFVVANAMVSFHNLLMIALQIFGGKMEFTGFRLLSVAILDMLN 114
Query: 61 STL 53
TL
Sbjct: 115 VTL 117
>At4g08690.1 68417.m01432 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650: CRAL/TRIO domain; similar to
polyphosphoinositide binding protein Ssh2p (GI:2739046)
[Glycine max]; similar to SEC14-like protein (GB:U82515)
[D. discoideum]
Length = 301
Score = 28.7 bits (61), Expect = 2.6
Identities = 18/59 (30%), Positives = 32/59 (54%), Gaps = 1/59 (1%)
Frame = +2
Query: 167 PNTRLQA-IYELRDMIYERGECTPHRMDDEFLIRFLRARNFIPQKAHRLLVNYYQFRAE 340
P QA I E+R ++ E D+ ++R+LRARN+ +KA ++L ++R +
Sbjct: 15 PTEEEQAKIEEVRKLLGPLPEKLSSFCSDDAVLRYLRARNWHVKKATKMLKETLKWRVQ 73
>At2g16380.1 68415.m01874 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to phosphatidylinositol
transfer-like protein III (GI:14486705) [Lotus
japonicus]
Length = 547
Score = 28.7 bits (61), Expect = 2.6
Identities = 14/37 (37%), Positives = 23/37 (62%), Gaps = 1/37 (2%)
Frame = +2
Query: 233 PHRMDD-EFLIRFLRARNFIPQKAHRLLVNYYQFRAE 340
P + DD ++RFLRAR F +KA ++ + Q+R +
Sbjct: 80 PPKHDDLHMMLRFLRARKFDKEKAKQMWSDMLQWRMD 116
>At1g01630.1 68414.m00080 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative contains
Pfam PF00650 : CRAL/TRIO domain and PF03765 : CRAL/TRIO,
N-terminus; similar to polyphosphoinositide binding
protein Ssh2p GB:AAB94599 GI:2739046 from [Glycine max]
Length = 255
Score = 28.3 bits (60), Expect = 3.4
Identities = 13/40 (32%), Positives = 23/40 (57%)
Frame = +2
Query: 215 ERGECTPHRMDDEFLIRFLRARNFIPQKAHRLLVNYYQFR 334
+R + +DD + RFLRAR+ +KA + +NY ++
Sbjct: 39 DRQDPETKEVDDLMIRRFLRARDLDIEKASTMFLNYLTWK 78
>At4g31540.1 68417.m04478 exocyst subunit EXO70 family protein
(EXO70-G1) tomato leucine zipper-containing protein -
Lycopersicon esculentum, PIR2:S21495; contains Pfam
domain PF03081: Exo70 exocyst complex subunit;
Length = 687
Score = 27.9 bits (59), Expect = 4.5
Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = -1
Query: 212 KSCPLVRRWLE--VEYWGPRRNADERSLGSLAGARRGPS 102
++C L +WLE VEY ADE+ L +L + RG S
Sbjct: 125 ENCGLAIQWLEDIVEYLDDHHVADEKYLSNLKKSLRGLS 163
>At1g22180.2 68414.m02774 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
profile: PF00650 CRAL/TRIO domain; similar to
polyphosphoinositide binding protein Ssh1p (GI:|2739044)
{Glycine max}; similar to Phosphatidylinositol Transfer
Protein Sec14p (GI:2780955) [Saccharomyces cerevisiae]
Length = 314
Score = 27.9 bits (59), Expect = 4.5
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = +2
Query: 167 PNTRLQAIYELRDMIYERGECTPHRMDDEFLIRFLRARNFIPQKAHRLLVNYYQFRAE 340
P L I E+R ++ E + D + R+L ARN +KA ++L ++RA+
Sbjct: 19 PEEYLNKINEVRTLLGPLTEKSSEFCSDAAITRYLAARNGHVKKATKMLKETLKWRAQ 76
>At1g56020.1 68414.m06431 expressed protein
Length = 398
Score = 27.5 bits (58), Expect = 5.9
Identities = 19/49 (38%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Frame = +2
Query: 95 SVDLDLGEPPPEIQD--YARQHCGEDPNTRLQAIYELRDMIYERGECTP 235
SVD +P PEIQD + C EDP T L A D ++ G+ P
Sbjct: 38 SVDKQQSDPLPEIQDPVVDFEFCLEDPVTMLSA-----DELFSDGKLVP 81
>At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to cytosolic factor
(Phosphatidylinositol/phosphatidylcholine transfer
protein) (PI/PCTP) (SP:P24280) [Saccharomyces
cerevisiae]; ESTs gb|T76582, gb|N06574 and gb|Z25700
come from this gene
Length = 540
Score = 27.1 bits (57), Expect = 7.8
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +2
Query: 248 DEFLIRFLRARNFIPQKAHRLLVNYYQFRAEN 343
D L++FLRAR+F +A +L ++R +N
Sbjct: 220 DVILLKFLRARDFKVNEAFEMLKKTLKWRKQN 251
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,995,028
Number of Sequences: 28952
Number of extensions: 252558
Number of successful extensions: 614
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 603
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 614
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 977150592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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