BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0548 (641 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 231 8e-62 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 113 2e-26 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 109 3e-25 SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 95 6e-21 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 94 2e-20 SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 93 3e-20 SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 84 2e-17 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 81 1e-16 SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 79 7e-16 SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 77 2e-15 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 66 4e-12 SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 59 6e-10 SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 56 6e-09 SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 38 0.001 SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ... 30 0.25 SPBC4B4.03 |rsc1||RSC complex subunit Rsc1 |Schizosaccharomyces ... 28 1.3 SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|... 28 1.3 SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizos... 27 3.0 SPAC824.03c |||conserved fungal protein|Schizosaccharomyces pomb... 25 9.3 SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||... 25 9.3 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 231 bits (564), Expect = 8e-62 Identities = 96/146 (65%), Positives = 120/146 (82%) Frame = +1 Query: 157 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKL 336 MS + RL EERK WR+DHPFGF A+P K+ DG L+LM W+ IPGK T WEGGLYKL Sbjct: 1 MSSLCKTRLQEERKQWRRDHPFGFYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKL 60 Query: 337 RMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLL 516 M F ++YP+ PPKC+F PPLFHPNVYPSGTVCLS+L+EE+ W+PAITIKQILLGIQDLL Sbjct: 61 TMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSILNEEEGWKPAITIKQILLGIQDLL 120 Query: 517 NEPNVKDPAQAEAYTIYCQNRLEYDK 594 ++PN+ PAQ EAYT++ ++++EY+K Sbjct: 121 DDPNIASPAQTEAYTMFKKDKVEYEK 146 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 113 bits (273), Expect = 2e-26 Identities = 58/144 (40%), Positives = 80/144 (55%) Frame = +1 Query: 157 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKL 336 MS A RL + K ++D P G A P+ + N+M W I G TP+E G +KL Sbjct: 1 MSTTARRRLMRDFKRMQQDPPAGVSASPVSD-----NVMLWNAVIIGPADTPFEDGTFKL 55 Query: 337 RMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLL 516 + F + YP+ PP KF +FHPNVY +G +CL +L + W P + IL IQ LL Sbjct: 56 VLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDIL--QNRWSPTYDVAAILTSIQSLL 113 Query: 517 NEPNVKDPAQAEAYTIYCQNRLEY 588 N+PN PA AEA ++ +N+ EY Sbjct: 114 NDPNNASPANAEAAQLHRENKKEY 137 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 109 bits (263), Expect = 3e-25 Identities = 59/152 (38%), Positives = 80/152 (52%), Gaps = 11/152 (7%) Frame = +1 Query: 166 IASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMI 345 +A RL +E K ++ P G A P D TW+C I G GTP+EGGLY + Sbjct: 5 MALRRLMKEYKELTENGPDGITAGPSNEDD----FFTWDCLIQGPDGTPFEGGLYPATLK 60 Query: 346 FKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKD-----------WRPAITIKQI 492 F DYP PP KFE FHPNVY GTVC+S+L D W P ++++I Sbjct: 61 FPSDYPLGPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKI 120 Query: 493 LLGIQDLLNEPNVKDPAQAEAYTIYCQNRLEY 588 LL + +L EPN + A +A ++ ++R EY Sbjct: 121 LLSVMSMLAEPNDESGANIDACKMWREDREEY 152 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 95.5 bits (227), Expect = 6e-21 Identities = 49/142 (34%), Positives = 76/142 (53%) Frame = +1 Query: 166 IASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMI 345 +A R+ E KD P A P+ + +L W+ I G +P+ GG++ L + Sbjct: 1 MALKRINRELADLGKDPPSSCSAGPVGD-----DLFHWQATIMGPADSPYAGGVFFLSIH 55 Query: 346 FKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLLNEP 525 F DYP PPK F ++HPN+ +G++CL +L ++ W PA+TI ++LL I LL +P Sbjct: 56 FPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQ--WSPALTISKVLLSICSLLTDP 113 Query: 526 NVKDPAQAEAYTIYCQNRLEYD 591 N DP E +Y +R Y+ Sbjct: 114 NPDDPLVPEIAHVYKTDRSRYE 135 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 93.9 bits (223), Expect = 2e-20 Identities = 44/115 (38%), Positives = 65/115 (56%) Frame = +1 Query: 250 PDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGT 429 PD NL+ W I G T +EG +K+ M F +YP SPP F P++HPNV SG Sbjct: 52 PDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGN 111 Query: 430 VCLSLLDEEKDWRPAITIKQILLGIQDLLNEPNVKDPAQAEAYTIYCQNRLEYDK 594 +CL +L ++ W ++ ILL +Q LL EPN P A+A ++ ++ +EY + Sbjct: 112 ICLDILKDK--WSAVYNVQTILLSLQSLLGEPNNASPLNAQAAELWSKDPIEYKR 164 >SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Schizosaccharomyces pombe|chr 1|||Manual Length = 148 Score = 93.1 bits (221), Expect = 3e-20 Identities = 47/133 (35%), Positives = 74/133 (55%) Frame = +1 Query: 178 RLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDD 357 R+ +E + +D P G VA P ++ NL ++ + G + + +EGG + L + D+ Sbjct: 6 RIIKEIETLTRDPPPGIVAAPTED-----NLRYFKITMEGPQQSAYEGGKFHLELFLPDE 60 Query: 358 YPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLLNEPNVKD 537 YP PP +F ++HPNV G +CLS L +KDW PA+ I+ +LL IQ L+ PN D Sbjct: 61 YPMMPPNVRFLTKIYHPNVDKLGRICLSTL--KKDWSPALQIRTVLLSIQALMGAPNPDD 118 Query: 538 PAQAEAYTIYCQN 576 P + I+ +N Sbjct: 119 PLDNDVAKIWKEN 131 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 83.8 bits (198), Expect = 2e-17 Identities = 48/156 (30%), Positives = 79/156 (50%), Gaps = 11/156 (7%) Frame = +1 Query: 160 SGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLR 339 S + L ++ K +K+ P GF + + ++ WE I G + T +EGG + Sbjct: 3 SSASEQLLRKQLKEIQKNPPQGFSVGLVDDK----SIFEWEVMIIGPEDTLYEGGFFHAT 58 Query: 340 MIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLL-----------DEEKDWRPAITIK 486 + F DYP PPK KF ++HPNV+P+G VC+S+L D + W P + + Sbjct: 59 LSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDAGERWLPVHSPE 118 Query: 487 QILLGIQDLLNEPNVKDPAQAEAYTIYCQNRLEYDK 594 IL+ + +L+ PN + PA +A + +N E+ K Sbjct: 119 TILISVISMLSSPNDESPANIDAAKEFRENPQEFKK 154 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 81.4 bits (192), Expect = 1e-16 Identities = 46/145 (31%), Positives = 73/145 (50%), Gaps = 1/145 (0%) Frame = +1 Query: 157 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKL 336 MS S R+A+E ++D G + + L M G +GTP+EGG + + Sbjct: 1 MSDNRSRRIAKELADVQQDKQAGIQVWTINDDISHLKGM-----FRGPEGTPYEGGYFVV 55 Query: 337 RMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCLSLLDEEKDWRPAITIKQILLGIQDL 513 + DYP PPK F+ ++HPNV +G +CL +L ++ W P T+K L+ +Q L Sbjct: 56 DIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQ--WSPVYTMKSALISLQSL 113 Query: 514 LNEPNVKDPAQAEAYTIYCQNRLEY 588 L P +P A+ +Y QN ++ Sbjct: 114 LCTPEPSNPQDAQVAQVYLQNYQQF 138 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 78.6 bits (185), Expect = 7e-16 Identities = 38/110 (34%), Positives = 58/110 (52%) Frame = +1 Query: 265 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 444 NL W C G + + GG + + F DYP PP +F ++HPN G VCL++ Sbjct: 35 NLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAI 94 Query: 445 LDEEKDWRPAITIKQILLGIQDLLNEPNVKDPAQAEAYTIYCQNRLEYDK 594 L +++ ++P+I ++ +L I LL EPN DP A Y +R +DK Sbjct: 95 L-KQQVFKPSIKLRSVLEQILQLLREPNPDDPLVASIAEQYRNDRPSFDK 143 >SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 77.0 bits (181), Expect = 2e-15 Identities = 33/81 (40%), Positives = 49/81 (60%) Frame = +1 Query: 313 WEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQI 492 ++GG +K R+ D+YP PPK K ++HPN+ G VCL++L +DW P + + I Sbjct: 65 YKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNIL--RQDWNPVLNLNSI 122 Query: 493 LLGIQDLLNEPNVKDPAQAEA 555 L+G+Q L PN +DP EA Sbjct: 123 LVGLQFLFLSPNAEDPLNKEA 143 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 66.1 bits (154), Expect = 4e-12 Identities = 32/103 (31%), Positives = 54/103 (52%), Gaps = 1/103 (0%) Frame = +1 Query: 250 PDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSG 426 P +++ W+ I G TP+EGG + L + + YP SPP F+ + HPN+ + +G Sbjct: 28 PVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNG 87 Query: 427 TVCLSLLDEEKDWRPAITIKQILLGIQDLLNEPNVKDPAQAEA 555 VC+ +L + W PA +++ L I LL+ + P +A Sbjct: 88 EVCMDIL--KTHWSPAWSLQSACLAIISLLSNYDASSPLNVDA 128 >SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 58.8 bits (136), Expect = 6e-10 Identities = 31/99 (31%), Positives = 49/99 (49%), Gaps = 2/99 (2%) Frame = +1 Query: 265 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYP-SGTVCLS 441 N+ + G TP+ GG++K+ + +YP P F +FHPN+ SG+VCL Sbjct: 28 NMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLD 87 Query: 442 LLDEEKDWRPAITIKQIL-LGIQDLLNEPNVKDPAQAEA 555 ++++ W P + I + + LL PN DP EA Sbjct: 88 VINQ--TWSPMFDMINIFEVFLPQLLRYPNASDPLNGEA 124 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 55.6 bits (128), Expect = 6e-09 Identities = 28/90 (31%), Positives = 41/90 (45%), Gaps = 1/90 (1%) Frame = +1 Query: 265 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 444 N++ W I G TP+EGG Y +IF DYP PP + P + +CLS Sbjct: 33 NILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP--SGRFQTNTRLCLSF 90 Query: 445 LD-EEKDWRPAITIKQILLGIQDLLNEPNV 531 D K W P+ + IL+G+ + + Sbjct: 91 SDFHPKSWNPSWMVSTILVGLVSFMTSDEI 120 >SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizosaccharomyces pombe|chr 3|||Manual Length = 139 Score = 37.9 bits (84), Expect = 0.001 Identities = 31/132 (23%), Positives = 57/132 (43%), Gaps = 2/132 (1%) Frame = +1 Query: 178 RLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDD 357 +L EE + K + + N D + L W I G + E +Y L++ + Sbjct: 9 KLLEELEKGEKGLGESSCSYGLTNAD-DITLSDWNATILGPAHSVHENRIYSLKIHCDAN 67 Query: 358 YPSSPPKCKFEPPLFHPNV-YPSGTVCLSLLDEEKDWRPAITIKQILLGI-QDLLNEPNV 531 YP +PP F + P V +G V +D + W+ +++ +LL + +++ + N Sbjct: 68 YPDAPPIVTFVSRINLPGVDGETGKVNPHKIDCLRHWKREYSMETVLLDLKKEMASSSNR 127 Query: 532 KDPAQAEAYTIY 567 K P E T + Sbjct: 128 KLPQPPEGSTFF 139 >SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 509 Score = 30.3 bits (65), Expect = 0.25 Identities = 20/58 (34%), Positives = 28/58 (48%), Gaps = 4/58 (6%) Frame = -1 Query: 386 NLHFGGLDG*SSLKIIRSLYSPPSHGVPFLPGIAHSQVI-KFNEPS---GFFIGLATK 225 N F G+ L + S SP HG P +PG+ H+ + + EPS F GL +K Sbjct: 442 NPQFSGVLNLPGLGTMPSFPSPYQHGQPQIPGMLHASLSNSYAEPSTQNSFIPGLTSK 499 >SPBC4B4.03 |rsc1||RSC complex subunit Rsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 27.9 bits (59), Expect = 1.3 Identities = 17/50 (34%), Positives = 23/50 (46%) Frame = -2 Query: 328 TAHLPMESPFYQVSHIPKSLNSMNHLDSS*ASRQNQRDGPYAKLSVLQLN 179 T+ +P PFY S +SLN+ H + + PYAKLS N Sbjct: 613 TSPIPAR-PFYAQSGSLQSLNTTQHSHQISGGHSGRMNVPYAKLSYTSHN 661 >SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 574 Score = 27.9 bits (59), Expect = 1.3 Identities = 12/36 (33%), Positives = 22/36 (61%) Frame = -1 Query: 215 WSLRQAFRSSAKRALAIPDIFYKNNSIYEPSFNQGL 108 W LR+ +S++ + ++F K NSIY +F++ L Sbjct: 71 WQLREQEQSNSHISRNENELFSKENSIYNGNFSENL 106 >SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 26.6 bits (56), Expect = 3.0 Identities = 12/39 (30%), Positives = 20/39 (51%) Frame = +1 Query: 217 PFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYK 333 P V+R + L+ TW C++ G+ G +G L+K Sbjct: 123 PSKVVSRKHAVVEYDLDDQTWNCSVYGRNGIKVDGKLFK 161 >SPAC824.03c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 25.0 bits (52), Expect = 9.3 Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 3/34 (8%) Frame = -1 Query: 548 ACAGSFTLGSLRRS*IPNK---ICFIVIAGRQSF 456 AC GSFT SL+ I N+ +C I + G S+ Sbjct: 137 ACLGSFTSSSLKTLSISNRPLALCRIYVDGFHSY 170 >SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2|||Manual Length = 1157 Score = 25.0 bits (52), Expect = 9.3 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = -1 Query: 473 AGRQSFSSSSSDKHTVPDGYTLGWNSGGSNL 381 A +QS+ + SSD ++P L +NS G + Sbjct: 249 ASQQSYINHSSDGASLPQSSFLNFNSSGKRV 279 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,616,593 Number of Sequences: 5004 Number of extensions: 57431 Number of successful extensions: 153 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 144 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 287744314 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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