Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0507
(423 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 119 9e-28
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 113 4e-26
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 113 5e-26
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 112 8e-26
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 58 2e-09
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 58 2e-09
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 40 7e-04
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 40 7e-04
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 39 0.001
At3g63520.1 68416.m07155 9-cis-epoxycarotenoid dioxygenase / neo... 29 1.3
At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 29 1.7
At3g16480.1 68416.m02103 mitochondrial processing peptidase alph... 28 3.0
At4g05490.1 68417.m00830 F-box family protein (FBL22) contains s... 27 5.2
At3g59050.1 68416.m06582 amine oxidase family protein similar to... 27 5.2
At1g51980.1 68414.m05863 mitochondrial processing peptidase alph... 27 5.2
At3g48880.2 68416.m05340 F-box family protein N7 protein - Medic... 27 6.9
At3g48880.1 68416.m05339 F-box family protein N7 protein - Medic... 27 6.9
At3g48110.2 68416.m05246 aminoacyl-t-RNA synthetase, putative si... 26 9.1
At3g48110.1 68416.m05245 aminoacyl-t-RNA synthetase, putative si... 26 9.1
At2g45540.1 68415.m05663 WD-40 repeat family protein / beige-rel... 26 9.1
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 119 bits (286), Expect = 9e-28
Identities = 61/89 (68%), Positives = 74/89 (83%)
Frame = -2
Query: 422 VETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGIDEDE 243
+E LR++AEADKNDK+VKDLV+LLYETALL+SGF+LDEP A+RI+RM+KLGL IDEDE
Sbjct: 621 MEELRKRAEADKNDKSVKDLVMLLYETALLTSGFSLDEPNTFAARIHRMLKLGLSIDEDE 680
Query: 242 PIQVEEPASGDVPPLEGDADDASRMEEVD 156
VEE GD+P LE DA + S+MEEVD
Sbjct: 681 --NVEE--DGDMPELEEDAAEESKMEEVD 705
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 113 bits (273), Expect = 4e-26
Identities = 55/90 (61%), Positives = 74/90 (82%), Gaps = 1/90 (1%)
Frame = -2
Query: 422 VETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGIDEDE 243
++ LR++A+ADKNDK+VKDLV+LL+ETALL+SGF+LDEP SRI+RM+KLGL ID+D+
Sbjct: 614 MDELRKRADADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFGSRIHRMLKLGLSIDDDD 673
Query: 242 PIQVEEPASGDVPPLEGDAD-DASRMEEVD 156
++ A D+PPLE DAD + S+MEEVD
Sbjct: 674 VVE----ADADMPPLEDDADAEGSKMEEVD 699
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 113 bits (272), Expect = 5e-26
Identities = 55/90 (61%), Positives = 74/90 (82%), Gaps = 1/90 (1%)
Frame = -2
Query: 422 VETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGIDEDE 243
++ LR++AEADKNDK+VKDLV+LL+ETALL+SGF+LDEP SRI+RM+KLGL I+ED+
Sbjct: 614 MDELRKRAEADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFGSRIHRMLKLGLSIEEDD 673
Query: 242 PIQVEEPASGDVPPLEGDAD-DASRMEEVD 156
++ A ++PPLE DAD + S+MEEVD
Sbjct: 674 AVE----ADAEMPPLEDDADAEGSKMEEVD 699
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 112 bits (270), Expect = 8e-26
Identities = 54/90 (60%), Positives = 74/90 (82%), Gaps = 1/90 (1%)
Frame = -2
Query: 422 VETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGIDEDE 243
++ LR++A+ADKNDK+VKDLV+LL+ETALL+SGF+LDEP SRI+RM+KLGL ID+D+
Sbjct: 614 MDELRKRADADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFGSRIHRMLKLGLSIDDDD 673
Query: 242 PIQVEEPASGDVPPLEGDAD-DASRMEEVD 156
++ A ++PPLE DAD + S+MEEVD
Sbjct: 674 AVE----ADAEMPPLEDDADAEGSKMEEVD 699
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 58.0 bits (134), Expect = 2e-09
Identities = 30/83 (36%), Positives = 49/83 (59%), Gaps = 5/83 (6%)
Frame = -2
Query: 422 VETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGID--- 252
++ L+ + +D D++VK+ L+Y+TAL+ SGF L +P+ A+RIY +K GL I
Sbjct: 717 IKELKDRIASDPEDESVKETAQLMYQTALIESGFILTDPKDFAARIYNSVKSGLNISPDA 776
Query: 251 -EDEPIQ-VEEPASGDVPPLEGD 189
DE I+ EEP + + + D
Sbjct: 777 VADEEIEAAEEPETSEATETKSD 799
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 58.0 bits (134), Expect = 2e-09
Identities = 30/83 (36%), Positives = 49/83 (59%), Gaps = 5/83 (6%)
Frame = -2
Query: 422 VETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGID--- 252
++ L+ + +D D++VK+ L+Y+TAL+ SGF L +P+ A+RIY +K GL I
Sbjct: 717 IKELKDRIASDPEDESVKETAQLMYQTALIESGFILTDPKDFAARIYNSVKSGLNISPDA 776
Query: 251 -EDEPIQ-VEEPASGDVPPLEGD 189
DE I+ EEP + + + D
Sbjct: 777 VADEEIEAAEEPETSEATETKSD 799
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 39.9 bits (89), Expect = 7e-04
Identities = 17/36 (47%), Positives = 26/36 (72%)
Frame = -2
Query: 365 LVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLG 258
+V LLY+TA++SSGFT D P ++IY M+ + +G
Sbjct: 700 VVDLLYDTAIISSGFTPDSPAELGNKIYEMMAMAVG 735
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 39.9 bits (89), Expect = 7e-04
Identities = 17/36 (47%), Positives = 26/36 (72%)
Frame = -2
Query: 365 LVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLG 258
+V LLY+TA++SSGFT D P ++IY M+ + +G
Sbjct: 703 VVDLLYDTAIISSGFTPDSPAELGNKIYEMMAMAVG 738
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 39.1 bits (87), Expect = 0.001
Identities = 23/82 (28%), Positives = 39/82 (47%), Gaps = 4/82 (4%)
Frame = -2
Query: 422 VETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGL----GI 255
++ + ++ ND+ + L+Y+ AL+SSGFT D P +IY M+ + L
Sbjct: 711 IKNINAAYNSNPNDEDAMRAIDLMYDAALVSSGFTPDNPAELGGKIYEMMDVALSGKWSS 770
Query: 254 DEDEPIQVEEPASGDVPPLEGD 189
E +P Q + S D E +
Sbjct: 771 PEVQPQQQQMAHSHDAETFEAE 792
>At3g63520.1 68416.m07155 9-cis-epoxycarotenoid dioxygenase /
neoxanthin cleavage enzyme / NCED1 / carotenoid cleavage
dioxygenase 1 (CCD1) identical to putative
9-cis-epoxy-carotenoid dioxygenase [GI:3096910];
contains Pfam profile PF03055: Retinal pigment
epithelial membrane protein
Length = 538
Score = 29.1 bits (62), Expect = 1.3
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -1
Query: 126 CHNGLFLCIGVQPKTDLVQKFHYFN 52
C NG F+ +G PK D V +H+F+
Sbjct: 74 CLNGEFVRVGPNPKFDAVAGYHWFD 98
>At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative
similar to delta-8 sphingolipid desaturase GI:3819708
from [Brassica napus]
Length = 449
Score = 28.7 bits (61), Expect = 1.7
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = +3
Query: 111 TNHYDMVPTVKVEILINLLHARCIIGITLQW 203
+ HY + T LI LL C+ GI++ W
Sbjct: 162 SGHYTVTSTKPCNKLIQLLSGNCLTGISIAW 192
>At3g16480.1 68416.m02103 mitochondrial processing peptidase alpha
subunit, putative similar to mitochondrial processing
peptidase alpha subunit, mitochondrial precursor,
Alpha-MPP (Ubiquinol-cytochrome C reductase subunit II)
[Potato] SWISS-PROT:P29677
Length = 499
Score = 27.9 bits (59), Expect = 3.0
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = -2
Query: 281 RMIKLGLGIDEDEPIQVEEPASGDVPPLEGDADDASR 171
RM+ G+D +E ++V EP D+P + A+ S+
Sbjct: 253 RMVLAASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQ 289
>At4g05490.1 68417.m00830 F-box family protein (FBL22) contains
similarity to N7 protein GI:3273101 from [Medicago
truncatula]
Length = 307
Score = 27.1 bits (57), Expect = 5.2
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = -3
Query: 259 VLMKMSRFRLKSLLLEMCRHWRVMPMMHLAWRRL 158
+L+++S + ++CR WR + L WRR+
Sbjct: 38 ILLRLSPLEILENARKVCRSWRRVSKDPLIWRRI 71
>At3g59050.1 68416.m06582 amine oxidase family protein similar to
polyamine oxidase (EC 1.5.3.11) precursor - Zea mays
[SP|O64411]; contains Pfam profile PF01593 amine
oxidase, flavin-containing
Length = 488
Score = 27.1 bits (57), Expect = 5.2
Identities = 14/51 (27%), Positives = 24/51 (47%)
Frame = -2
Query: 404 KAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASRIYRMIKLGLGID 252
K +K D V D ++ +L SG EP++ + + LG+GI+
Sbjct: 258 KVTTEKGDTFVADAAVIALPLGVLKSGMITFEPKLPQWKQEAINDLGVGIE 308
>At1g51980.1 68414.m05863 mitochondrial processing peptidase alpha
subunit, putative similar to mitochondrial processing
peptidase alpha subunit, mitochondrial precursor,
Alpha-MPP (Ubiquinol-cytochrome C reductase subunit II)
[Potato] SWISS-PROT:P29677
Length = 503
Score = 27.1 bits (57), Expect = 5.2
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = -2
Query: 329 SGFTLDEPQVHASRIYRMIKLGLGIDEDEPIQVEEPASGDVP 204
+G L+E RM+ G++ +E ++V EP + D+P
Sbjct: 241 NGELLEEFMTENFTAARMVLAASGVEHEELLKVAEPLTSDLP 282
>At3g48880.2 68416.m05340 F-box family protein N7 protein - Medicago
truncatula, EMBL:CAA76808 ; similar to SKP1 interacting
partner 2 (SKIP2) TIGR_Ath1:At5g67250
Length = 309
Score = 26.6 bits (56), Expect = 6.9
Identities = 12/34 (35%), Positives = 16/34 (47%)
Frame = -3
Query: 265 VLVLMKMSRFRLKSLLLEMCRHWRVMPMMHLAWR 164
V + K S F L S L +CR WR + W+
Sbjct: 22 VRIFQKFSVFELTSGLAHVCRGWRAACCDPILWK 55
>At3g48880.1 68416.m05339 F-box family protein N7 protein - Medicago
truncatula, EMBL:CAA76808 ; similar to SKP1 interacting
partner 2 (SKIP2) TIGR_Ath1:At5g67250
Length = 309
Score = 26.6 bits (56), Expect = 6.9
Identities = 12/34 (35%), Positives = 16/34 (47%)
Frame = -3
Query: 265 VLVLMKMSRFRLKSLLLEMCRHWRVMPMMHLAWR 164
V + K S F L S L +CR WR + W+
Sbjct: 22 VRIFQKFSVFELTSGLAHVCRGWRAACCDPILWK 55
>At3g48110.2 68416.m05246 aminoacyl-t-RNA synthetase, putative
similar to aminoacyl-t-RNA synthetase GI:2654226 from
[Arabidopsis thaliana]; contains Pfam profiles: PF02092
glycyl-tRNA synthetase beta subunit,PF02091 glycyl-tRNA
synthetase alpha subunit
Length = 1063
Score = 26.2 bits (55), Expect = 9.1
Identities = 25/99 (25%), Positives = 44/99 (44%), Gaps = 2/99 (2%)
Frame = +3
Query: 120 YDMVPTVKVEILINLLHARCIIGITLQWRHISRSR-LFNLNRLIFINTKTQFDHSIDTGS 296
YD + +K N+L AR IG+T + R+ R R L +++ T+ H + S
Sbjct: 303 YDQL--LKTSHAFNILDARGFIGVTERARYFGRMRSLARQCAQLWLATRESLGHPLGVAS 360
Query: 297 MDL-GLIKREARRQQGSFIQ*DNKILNSFVILVGFRFLP 410
+ + R A + + D + SF+I +G +P
Sbjct: 361 EPVPPVCHRAALEKVAEKVSEDPR---SFIIEIGTEEMP 396
>At3g48110.1 68416.m05245 aminoacyl-t-RNA synthetase, putative
similar to aminoacyl-t-RNA synthetase GI:2654226 from
[Arabidopsis thaliana]; contains Pfam profiles: PF02092
glycyl-tRNA synthetase beta subunit,PF02091 glycyl-tRNA
synthetase alpha subunit
Length = 1064
Score = 26.2 bits (55), Expect = 9.1
Identities = 25/99 (25%), Positives = 44/99 (44%), Gaps = 2/99 (2%)
Frame = +3
Query: 120 YDMVPTVKVEILINLLHARCIIGITLQWRHISRSR-LFNLNRLIFINTKTQFDHSIDTGS 296
YD + +K N+L AR IG+T + R+ R R L +++ T+ H + S
Sbjct: 303 YDQL--LKTSHAFNILDARGFIGVTERARYFGRMRSLARQCAQLWLATRESLGHPLGVAS 360
Query: 297 MDL-GLIKREARRQQGSFIQ*DNKILNSFVILVGFRFLP 410
+ + R A + + D + SF+I +G +P
Sbjct: 361 EPVPPVCHRAALEKVAEKVSEDPR---SFIIEIGTEEMP 396
>At2g45540.1 68415.m05663 WD-40 repeat family protein / beige-related
contains Pfam PF02138: Beige/BEACH domain; contains Pfam
PF00400: WD domain, G-beta repeat (3 copies)
Length = 2946
Score = 26.2 bits (55), Expect = 9.1
Identities = 13/35 (37%), Positives = 23/35 (65%), Gaps = 2/35 (5%)
Frame = +2
Query: 215 QKQALQPE--SAHLHQYQDPV*SFYRYGKHGLGAH 313
Q++AL+ E SAHLH++ D + + + GK + A+
Sbjct: 2427 QRRALESEHVSAHLHEWIDLIFGYKQRGKEAIMAN 2461
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,101,601
Number of Sequences: 28952
Number of extensions: 152539
Number of successful extensions: 382
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 366
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 378
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 655255392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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