Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0472
(398 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g26750.1 68416.m03346 expressed protein 29 1.5
At5g23350.1 68418.m02731 GRAM domain-containing protein / ABA-re... 28 2.0
At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein... 28 2.6
At5g26020.1 68418.m03096 hypothetical protein 27 3.5
At1g66980.1 68414.m07616 protein kinase family protein / glycero... 27 3.5
At4g34490.1 68417.m04903 cyclase-associated protein (cap1) ident... 27 4.6
At3g16830.1 68416.m02149 WD-40 repeat family protein contains 10... 27 4.6
At1g76920.1 68414.m08954 F-box family protein (FBX3) contains si... 27 4.6
At3g66652.1 68416.m00776 fip1 motif-containing protein contains ... 27 6.1
At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70... 26 8.0
At4g13050.1 68417.m02036 acyl-[acyl carrier protein] thioesteras... 26 8.0
>At3g26750.1 68416.m03346 expressed protein
Length = 526
Score = 28.7 bits (61), Expect = 1.5
Identities = 19/58 (32%), Positives = 30/58 (51%)
Frame = +1
Query: 208 TGSSTDTLRKLSDDLSRKHLSDLIEAEEDEVSSSLPDKPRTYRKASNHSVILTEDTDS 381
TG S+D RK + L R + L+E ++E+S + K T + + VIL +T S
Sbjct: 393 TGESSDVFRKYT--LERMFTNQLVECSKEELSFHVLVKDLTTKSPLFNIVILNPNTFS 448
>At5g23350.1 68418.m02731 GRAM domain-containing protein /
ABA-responsive protein-related contains similarity to
ABA-responsive protein in barley (GI:4103635) [Hordeum
vulgare] [J. Exp. Bot. 50, 727-728 (1999); contains Pfam
PF02893: GRAM domain
Length = 280
Score = 28.3 bits (60), Expect = 2.0
Identities = 15/57 (26%), Positives = 27/57 (47%)
Frame = +3
Query: 45 RICITQVGTTEASTPRYNHKLRFPNFKSRTNSIIQRNFFFENHKMPVETKISFSDGQ 215
RI IT + + + + FP K+ T S + NH++P +K+S+ G+
Sbjct: 53 RIKITTQSSKMTLSRVHQQVIAFPAAKTDTMSYLPDPASINNHQIPTSSKVSYLTGK 109
>At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein
contains Pfam domain PF00098: Zinc knuckle
Length = 474
Score = 27.9 bits (59), Expect = 2.6
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +3
Query: 153 NFFFENHKMPVETKISFSDGQLDGHA 230
N++FE H E K+S + GQL G A
Sbjct: 127 NYYFEFHSTAQEDKLSIALGQLKGSA 152
>At5g26020.1 68418.m03096 hypothetical protein
Length = 241
Score = 27.5 bits (58), Expect = 3.5
Identities = 17/54 (31%), Positives = 22/54 (40%)
Frame = +1
Query: 187 KPKLAFPTGSSTDTLRKLSDDLSRKHLSDLIEAEEDEVSSSLPDKPRTYRKASN 348
KPK F + DD D ++ +ED SS P+K R Y K N
Sbjct: 154 KPKKVFEISDEDEDDDDEEDDYGDNGADD-VDDDEDNGKSSKPNKRRQYSKVWN 206
>At1g66980.1 68414.m07616 protein kinase family protein /
glycerophosphoryl diester phosphodiesterase family
protein similar to leaf rust resistance kinase Lr10
GI:1680685 from [Triticum aestivum]; contains Pfam
profiles PF03009: Glycerophosphoryl diester
phosphodiesterase family, PF00069: Protein kinase domain
Length = 1109
Score = 27.5 bits (58), Expect = 3.5
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 7/63 (11%)
Frame = +1
Query: 145 SKGIFFLKIIRCPSKPKLAFPTGSSTDTL-------RKLSDDLSRKHLSDLIEAEEDEVS 303
S G+ L+II +K K S+T ++ R L S +H+ D I +EEDE++
Sbjct: 983 SYGMLVLEIIGARNKEKANQACASNTSSMYFPEWVYRDLESCKSGRHIEDGINSEEDELA 1042
Query: 304 SSL 312
+
Sbjct: 1043 KKM 1045
>At4g34490.1 68417.m04903 cyclase-associated protein (cap1)
identical to cyclase-associated protein (cap1)
GI:3169136 from [Arabidopsis thaliana]
Length = 476
Score = 27.1 bits (57), Expect = 4.6
Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 3/44 (6%)
Frame = +1
Query: 208 TGSSTDTLRKLSDDLSRKHLSD---LIEAEEDEVSSSLPDKPRT 330
+G+ T LRK++DD+ K+ +D + A E E +S P +T
Sbjct: 271 SGAVTSGLRKVTDDMKTKNRADRSGAVSAVEKETRTSKPAFSKT 314
>At3g16830.1 68416.m02149 WD-40 repeat family protein contains 10
WD-40 repeats (PF00400) (1 weak)
Length = 1131
Score = 27.1 bits (57), Expect = 4.6
Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 2/51 (3%)
Frame = +3
Query: 24 FNDTHDPR-ICITQVGTTEASTPRYNHKLRFPNFK-SRTNSIIQRNFFFEN 170
+ DT R I T++ + P + KL FP+FK SR ++I ++ +++
Sbjct: 133 YGDTKSARSIMYTELKKLIEANPLFREKLAFPSFKASRLRTLINQSLNWQH 183
>At1g76920.1 68414.m08954 F-box family protein (FBX3) contains
similarity to stamina pistilloidia GI:4101570, the pea
ortholog of Fim and UFO from [Pisum sativum]
Length = 374
Score = 27.1 bits (57), Expect = 4.6
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2
Query: 38 RPAHLHHAGRHHGSEHS 88
RP H HH+ RH G+ H+
Sbjct: 61 RPPHHHHSHRHSGNGHA 77
>At3g66652.1 68416.m00776 fip1 motif-containing protein contains
Pfam profile PF05182: Fip1 motif
Length = 980
Score = 26.6 bits (56), Expect = 6.1
Identities = 17/59 (28%), Positives = 29/59 (49%)
Frame = +1
Query: 205 PTGSSTDTLRKLSDDLSRKHLSDLIEAEEDEVSSSLPDKPRTYRKASNHSVILTEDTDS 381
PT SS+ + + SDD + L D ++ EVS+ P+ R A + ++ + DS
Sbjct: 349 PTSSSSVGINEESDDYETESLKDSATDDQREVST----PPQEARLAEHEAISIKRGEDS 403
>At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein 70
[Arabidopsis thaliana] GI:6746592; similar to heat shock
70 protein - Spinacia oleracea,PID:g2654208
Length = 718
Score = 26.2 bits (55), Expect = 8.0
Identities = 19/56 (33%), Positives = 24/56 (42%)
Frame = +1
Query: 172 IRCPSKPKLAFPTGSSTDTLRKLSDDLSRKHLSDLIEAEEDEVSSSLPDKPRTYRK 339
+ CP+ K S LRKL DD SR L+D + V + D RT K
Sbjct: 177 LECPAINKQFAAEEISAQVLRKLVDDASR-FLNDKVTKAVITVPAYFNDSQRTATK 231
>At4g13050.1 68417.m02036 acyl-[acyl carrier protein] thioesterase,
putative / acyl-ACP thioesterase, putative /
oleoyl-[acyl-carrier protein] hydrolase, putative /
S-acyl fatty acid synthase thioesterase, putative strong
similarity to acyl-ACP thioesterase;
oleoyl-[acyl-carrier protein] hydrolase [Brassica napus]
GI:435011; contains Pfam profile PF01643: Acyl-ACP
thioesterase
Length = 367
Score = 26.2 bits (55), Expect = 8.0
Identities = 12/28 (42%), Positives = 17/28 (60%), Gaps = 2/28 (7%)
Frame = +1
Query: 169 IIRCPSKPKLAFP--TGSSTDTLRKLSD 246
++ CP +P+LAFP SS + KL D
Sbjct: 217 LVFCPPEPRLAFPEENNSSLKKIPKLED 244
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,662,941
Number of Sequences: 28952
Number of extensions: 134154
Number of successful extensions: 488
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 471
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 485
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 575830496
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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