Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0443
(534 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g20360.1 68415.m02377 expressed protein 110 7e-25
At1g19540.1 68414.m02434 isoflavone reductase, putative similar ... 36 0.017
At1g75290.1 68414.m08746 isoflavone reductase, putative similar ... 33 0.12
At1g75280.1 68414.m08745 isoflavone reductase, putative identica... 33 0.12
At2g44040.1 68415.m05475 dihydrodipicolinate reductase family pr... 32 0.21
At1g75300.1 68414.m08747 isoflavone reductase, putative identica... 32 0.21
At2g45310.1 68415.m05639 NAD-dependent epimerase/dehydratase fam... 31 0.64
At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondri... 29 1.5
At3g59890.2 68416.m06683 dihydrodipicolinate reductase family pr... 29 1.5
At3g59890.1 68416.m06684 dihydrodipicolinate reductase family pr... 29 1.5
At3g03980.1 68416.m00419 short-chain dehydrogenase/reductase (SD... 29 1.9
At4g31600.1 68417.m04489 UDP-glucuronic acid/UDP-N-acetylgalacto... 29 2.6
At2g34070.1 68415.m04171 expressed protein 29 2.6
At5g19060.1 68418.m02266 expressed protein ; expression supporte... 28 3.4
At4g11370.1 68417.m01833 zinc finger (C3HC4-type RING finger) fa... 28 3.4
At3g46810.1 68416.m05081 DC1 domain-containing protein contains ... 28 4.5
At1g02000.1 68414.m00118 NAD-dependent epimerase/dehydratase fam... 28 4.5
At5g54960.1 68418.m06845 pyruvate decarboxylase, putative strong... 27 7.9
At5g50310.1 68418.m06229 kelch repeat-containing protein similar... 27 7.9
At5g44400.1 68418.m05436 FAD-binding domain-containing protein s... 27 7.9
At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family pr... 27 7.9
>At2g20360.1 68415.m02377 expressed protein
Length = 402
Score = 110 bits (264), Expect = 7e-25
Identities = 56/120 (46%), Positives = 85/120 (70%)
Frame = +2
Query: 173 HLNGSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYVCNKLG 352
HLNG+ + Y +++ ++ +LA ++GTGGRSS +GIVATVFG TGF+GRY+ +L
Sbjct: 34 HLNGTDNCRY--SSSLATKGVGHLA--RKGTGGRSSVSGIVATVFGATGFLGRYLVQQLA 89
Query: 353 KIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAKAVRYSNVVINLVGRDY 532
K+G+Q+++P+RG + LK+ GDLGQV+ + DE+SI + +NVVINL+GR+Y
Sbjct: 90 KMGSQVLVPFRGSEDSPRHLKLMGDLGQVVPMKFDPRDEDSIKAVMAKANVVINLIGREY 149
>At1g19540.1 68414.m02434 isoflavone reductase, putative similar to
SP|P52577; contains isoflavone reductase domain PF02716
Length = 310
Score = 35.9 bits (79), Expect = 0.017
Identities = 26/79 (32%), Positives = 39/79 (49%), Gaps = 4/79 (5%)
Frame = +2
Query: 302 VFGCTGFVGRYVCNKLGKIGTQLILPYR----GDFYDAQRLKVCGDLGQVLFTPYHLLDE 469
V G TG +G+ + + K G R D AQ ++ DLG + L D+
Sbjct: 7 VIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYG-SLSDK 65
Query: 470 ESIAKAVRYSNVVINLVGR 526
ES+ KA++ +VVI+ VGR
Sbjct: 66 ESLVKAIKQVDVVISAVGR 84
>At1g75290.1 68414.m08746 isoflavone reductase, putative similar to
SP|P52577 Isoflavone reductase homolog P3 (EC 1.3.1.-)
{Arabidopsis thaliana}; contains Pfam profile PF02716:
Isoflavone reductase
Length = 323
Score = 33.1 bits (72), Expect = 0.12
Identities = 24/77 (31%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +2
Query: 302 VFGCTGFVGRYVCNKLGKIG-TQLILPYRGDFYDAQRLKVCGDLGQVLFTPYH--LLDEE 472
V G TG +G+ + K G + L L D + K + T H L D E
Sbjct: 10 VIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGDLNDHE 69
Query: 473 SIAKAVRYSNVVINLVG 523
S+ KA++ ++VVI+ VG
Sbjct: 70 SLVKAIKQADVVISTVG 86
>At1g75280.1 68414.m08745 isoflavone reductase, putative identical
to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: isoflavone reductase
Length = 310
Score = 33.1 bits (72), Expect = 0.12
Identities = 23/77 (29%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +2
Query: 302 VFGCTGFVGRYVCNKLGKIG-TQLILPYRGDFYDAQRLKVCGDLGQVLFTPYH--LLDEE 472
V G TG++G+++ K G + L D + K + T H L D E
Sbjct: 10 VIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLNDHE 69
Query: 473 SIAKAVRYSNVVINLVG 523
S+ KA++ +VVI+ VG
Sbjct: 70 SLVKAIKQVDVVISTVG 86
>At2g44040.1 68415.m05475 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae} ;
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 347
Score = 32.3 bits (70), Expect = 0.21
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +2
Query: 284 NGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYDAQRLKVCG 424
NGI V GC+G +G+ V G ++ + + D QR++VCG
Sbjct: 71 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPISFGSAGEDGQRVEVCG 118
>At1g75300.1 68414.m08747 isoflavone reductase, putative identical
to SP|P52577 Isoflavone reductase homolog P3 (EC
1.3.1.-) {Arabidopsis thaliana}; contains Pfam profile
PF02716: Isoflavone reductase
Length = 322
Score = 32.3 bits (70), Expect = 0.21
Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 4/78 (5%)
Frame = +2
Query: 302 VFGCTGFVGRYVCNKLGKIGTQLILPYR----GDFYDAQRLKVCGDLGQVLFTPYHLLDE 469
V G TG++G ++ K G R D ++ ++ DLG V L D
Sbjct: 10 VIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLG-VTILHGDLNDH 68
Query: 470 ESIAKAVRYSNVVINLVG 523
ES+ KA++ +VVI+ +G
Sbjct: 69 ESLVKAIKQVDVVISTIG 86
>At2g45310.1 68415.m05639 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam profile
PF01370 NAD dependent epimerase/dehydratase family
Length = 437
Score = 30.7 bits (66), Expect = 0.64
Identities = 18/43 (41%), Positives = 23/43 (53%), Gaps = 1/43 (2%)
Frame = +2
Query: 275 SSFNGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYD 400
S+ NGI V G GFVG +V L + G +I L D+YD
Sbjct: 92 STNNGITVLVTGAAGFVGTHVSAALKRRGDGVIGLDNFNDYYD 134
>At5g13490.1 68418.m01556 ADP, ATP carrier protein 2, mitochondrial
/ ADP/ATP translocase 2 / adenine nucleotide
translocator 2 (ANT2) identical to SWISS-PROT:P40941
ADP,ATP carrier protein 2, mitochondrial precursor
(Adenine nucleotide translocator 2) [Arabidopsis
thaliana]
Length = 385
Score = 29.5 bits (63), Expect = 1.5
Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 7/100 (7%)
Frame = +2
Query: 182 GSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIV---ATVFGCTGFVGRYVCNKLG 352
G+ S++++ + +Y+ R N + + GG FNG+V G G Y +
Sbjct: 200 GASSLLFVYSLDYARTRLANDSKSAKKGGGERQFNGLVDVYKKTLKSDGIAGLYRGFNIS 259
Query: 353 KIGTQLILPYRG---DFYDAQR-LKVCGDLGQVLFTPYHL 460
G I+ YRG YD+ + + + GDL F + L
Sbjct: 260 CAG---IIVYRGLYFGLYDSVKPVLLTGDLQDSFFASFAL 296
>At3g59890.2 68416.m06683 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae};
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 343
Score = 29.5 bits (63), Expect = 1.5
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +2
Query: 284 NGIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDA-QRLKVCG 424
NGI V GC+G +G+ V G ++ G +A Q ++VCG
Sbjct: 67 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPTSFGSVEEAGQTVEVCG 114
>At3g59890.1 68416.m06684 dihydrodipicolinate reductase family
protein weak similarity to SP|Q52419 Dihydrodipicolinate
reductase (EC 1.3.1.26) (DHPR) {Pseudomonas syringae};
contains Pfam profiles PF01113: Dihydrodipicolinate
reductase N-terminus, PF05173: Dihydrodipicolinate
reductase C-terminus
Length = 349
Score = 29.5 bits (63), Expect = 1.5
Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +2
Query: 284 NGIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDA-QRLKVCG 424
NGI V GC+G +G+ V G ++ G +A Q ++VCG
Sbjct: 73 NGISIMVNGCSGKMGKAVIKAADSAGVNIVPTSFGSVEEAGQTVEVCG 120
>At3g03980.1 68416.m00419 short-chain dehydrogenase/reductase (SDR)
family protein similar to short-chain type
dehydrogenase/reductase SP:Q08632 [Picea abies]
Length = 270
Score = 29.1 bits (62), Expect = 1.9
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = +2
Query: 287 GIVATVFGCTGFVGRYVCNKLGKIGTQLILPYRGDFYDAQRL 412
G VA V G + +GR + L ++G ++++ Y DA+R+
Sbjct: 16 GRVAIVTGSSRGIGRAIAIHLAELGARIVINYTSKAADAERV 57
>At4g31600.1 68417.m04489 UDP-glucuronic
acid/UDP-N-acetylgalactosamine transporter-related
contains weak similarity to UDP-glucuronic
acid/UDP-N-acetylgalactosamine transporter (UDP-
GlcA/UDP-GalNAc transporter) (Swiss-Prot:Q9NTN3) [Homo
sapiens]
Length = 323
Score = 28.7 bits (61), Expect = 2.6
Identities = 17/62 (27%), Positives = 31/62 (50%)
Frame = +2
Query: 50 ALSLSILNGSIMKVYK*ELSQYLSHKMAAIALKTQATSKLLHLNGSMSVVYIKAANYSSD 229
A+S I + +++ + K + QY H M + L+ ATS L+H M K + ++
Sbjct: 18 AVSYGIASMAMVFINKAVIMQY-PHSMTVLTLQQLATSLLIHFGRRMGYTRAKGIDMATA 76
Query: 230 RK 235
+K
Sbjct: 77 KK 78
>At2g34070.1 68415.m04171 expressed protein
Length = 385
Score = 28.7 bits (61), Expect = 2.6
Identities = 19/55 (34%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
Frame = +2
Query: 176 LNGS-MSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYV 337
L+G+ ++ Y+K + S K LAA GG+ G T GC F GR+V
Sbjct: 20 LSGADQALAYVKKPHVSQRNKTALAAVAGRGGGKEMLKGRKQT-SGCNLFQGRWV 73
>At5g19060.1 68418.m02266 expressed protein ; expression supported
by MPSS
Length = 551
Score = 28.3 bits (60), Expect = 3.4
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -2
Query: 293 RCR*SYCGHQYLAYMLQGWVYDPNCS*RL 207
RCR +C A GWVY +CS +L
Sbjct: 272 RCREPWCDGALSALESNGWVYSSHCSFKL 300
>At4g11370.1 68417.m01833 zinc finger (C3HC4-type RING finger)
family protein strong similarity to RING-H2 finger
protein RHA1a [Arabidopsis thaliana] GI:3790554;
contains Pfam profile PF00097: Zinc finger, C3HC4 type
(RING finger)
Length = 159
Score = 28.3 bits (60), Expect = 3.4
Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 2/81 (2%)
Frame = -2
Query: 458 DGKE*TGPGLNLRTLSTFGHHRNRLC-MVKLTGYQFFPI-CCTHSVRQIRCNRKLWLRCR 285
D E + P L LST N L +V+ + P CCT + + K+ +
Sbjct: 45 DHNETSAPDLTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPK 104
Query: 284 *SYCGHQYLAYMLQGWVYDPN 222
CGH + Y L W+ D N
Sbjct: 105 ---CGHVFHHYCLDRWIVDYN 122
>At3g46810.1 68416.m05081 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 686
Score = 27.9 bits (59), Expect = 4.5
Identities = 16/66 (24%), Positives = 28/66 (42%)
Frame = +2
Query: 317 GFVGRYVCNKLGKIGTQLILPYRGDFYDAQRLKVCGDLGQVLFTPYHLLDEESIAKAVRY 496
GF G Y C+K + R D +D L+ G + P+ ++DE +I
Sbjct: 328 GFYGAYSCSKCSSYVVHSLCATRKDVWDMVELE--GTPEEEEIAPFEVVDENTIKHMSHD 385
Query: 497 SNVVIN 514
+++ N
Sbjct: 386 HDLIFN 391
>At1g02000.1 68414.m00118 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam profile
PF01370 NAD dependent epimerase/dehydratase family
Length = 434
Score = 27.9 bits (59), Expect = 4.5
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = +2
Query: 284 NGIVATVFGCTGFVGRYVCNKLGKIGTQLI-LPYRGDFYD 400
NG+ V G GFVG +V L + G ++ L D+YD
Sbjct: 90 NGVSVLVTGAAGFVGTHVSAALKRRGDGVLGLDNFNDYYD 129
>At5g54960.1 68418.m06845 pyruvate decarboxylase, putative strong
similarity to pyruvate decarboxylase 1 [Vitis vinifera]
GI:10732644; contains InterPro entry IPR000399: Pyruvate
decarboxylase
Length = 607
Score = 27.1 bits (57), Expect = 7.9
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = +2
Query: 299 TVFGCTGFVGRYVCNKLGKIGTQLILPYRGDF 394
TV C +GRY+ +L +IG + GDF
Sbjct: 39 TVSPCDATLGRYLARRLVEIGVTDVFSVPGDF 70
>At5g50310.1 68418.m06229 kelch repeat-containing protein similar to
Kelch repeats protein 3 (SP:Q08979) [Saccharomyces
cerevisiae]; contains Pfam PF01344: Kelch motif (6
repeats)
Length = 666
Score = 27.1 bits (57), Expect = 7.9
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +2
Query: 362 TQLILPYRGDFYDAQRLKVCGDL 430
T+LIL Y G+FY+ Q+ V GDL
Sbjct: 82 TELIL-YGGEFYNGQKTYVYGDL 103
>At5g44400.1 68418.m05436 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 537
Score = 27.1 bits (57), Expect = 7.9
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = -2
Query: 365 GYQFFPICCTHSVRQIRCNRKLWLRCR*SYCGHQY--LAYMLQ 243
G+ F PI +H I C++KL + R GH Y L+Y+ Q
Sbjct: 85 GFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQ 127
>At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain ;annotation temporarily based on
supporting cDNA gi|17224394|gb|AF246291.1|AF246291
Length = 637
Score = 27.1 bits (57), Expect = 7.9
Identities = 16/68 (23%), Positives = 29/68 (42%)
Frame = +2
Query: 182 GSMSVVYIKAANYSSDRKPNLAAYKRGTGGRSSFNGIVATVFGCTGFVGRYVCNKLGKIG 361
G+M++ K + N A + G G + S N + TGF G ++G G
Sbjct: 506 GTMTMKRKKPCDAGERTSANCANNETGNGKKVSVNNVGEAEPADTGFTGLTDNLRIGSFG 565
Query: 362 TQLILPYR 385
++++ R
Sbjct: 566 NEVVIELR 573
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,471,846
Number of Sequences: 28952
Number of extensions: 229640
Number of successful extensions: 560
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 531
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 560
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 984125600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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