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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= P5PG0430
         (557 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g67280.1 68414.m07657 lactoylglutathione lyase, putative / gl...    82   2e-16
At1g11840.3 68414.m01360 lactoylglutathione lyase, putative / gl...    73   1e-13
At1g11840.2 68414.m01362 lactoylglutathione lyase, putative / gl...    73   1e-13
At1g11840.1 68414.m01361 lactoylglutathione lyase, putative / gl...    73   1e-13
At1g08110.2 68414.m00889 lactoylglutathione lyase, putative / gl...    32   0.30 
At1g08110.1 68414.m00888 lactoylglutathione lyase, putative / gl...    32   0.30 
At2g26680.1 68415.m03200 expressed protein similar to NLPE1 (GI:...    29   2.1  
At4g23090.1 68417.m03328 hypothetical protein                          28   4.9  
At1g31530.1 68414.m03867 endonuclease/exonuclease/phosphatase fa...    27   8.5  

>At1g67280.1 68414.m07657 lactoylglutathione lyase, putative /
           glyoxalase I, putative similar to putative
           lactoylglutathione lyase SP:Q39366, GI:2494843 from
           [Brassica oleracea]
          Length = 350

 Score = 82.2 bits (194), Expect = 2e-16
 Identities = 48/131 (36%), Positives = 69/131 (52%), Gaps = 11/131 (8%)
 Frame = +2

Query: 194 RALHFVFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPED 373
           R LH V++V D   T KFY E LGMK+LR  +  E            +++   +GYGPED
Sbjct: 88  RMLHVVYRVGDMDRTIKFYTECLGMKLLRKRDIPE-----------EKYTNAFLGYGPED 136

Query: 374 THFVVELTYNYGVTHYEQGNDF--LGITVQS-SESLKRAQTNNWPIKEHNG--------L 520
           +HFV+ELTYNYGV  Y+ G  F   GI V   +++++  +     +    G        +
Sbjct: 137 SHFVIELTYNYGVDKYDIGAGFGHFGIAVDDVAKTVELVKAKGGKVSREPGPVKGGKTVI 196

Query: 521 KYVEAPGGYKF 553
            ++E P GYKF
Sbjct: 197 AFIEDPDGYKF 207



 Score = 62.5 bits (145), Expect = 2e-10
 Identities = 34/92 (36%), Positives = 52/92 (56%)
 Frame = +2

Query: 209 VFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPEDTHFVV 388
           + +V D     KFY +  GM++LR  +         N  Y  +++  M+GYGPED   V+
Sbjct: 223 MLRVGDLDRAIKFYEKAFGMELLRTRD---------NPEY--KYTIAMMGYGPEDKFPVL 271

Query: 389 ELTYNYGVTHYEQGNDFLGITVQSSESLKRAQ 484
           ELTYNYGVT Y++GN +  I + + +  K A+
Sbjct: 272 ELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAE 303


>At1g11840.3 68414.m01360 lactoylglutathione lyase, putative /
           glyoxalase I, putative highly similar to putative
           lactoylglutathione lyase SP:Q39366 from [Brassica
           oleracea]
          Length = 232

 Score = 72.9 bits (171), Expect = 1e-13
 Identities = 48/131 (36%), Positives = 68/131 (51%), Gaps = 11/131 (8%)
 Frame = +2

Query: 194 RALHFVFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPED 373
           R LH V++V D   T +FY E+ GMK+LR  +  E            ++S   +G+GPE 
Sbjct: 17  RFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPE-----------EKYSNAFLGFGPET 65

Query: 374 THFVVELTYNYGVTHYEQGNDF--LGITVQSSESL---KRAQTNN-----WPIKEHNG-L 520
           ++FVVELTYNYGV+ Y+ G  F    I+ Q    L    RA+  N      P+K     +
Sbjct: 66  SNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVI 125

Query: 521 KYVEAPGGYKF 553
            +V+ P GY F
Sbjct: 126 AFVKDPDGYTF 136



 Score = 48.4 bits (110), Expect = 3e-06
 Identities = 29/89 (32%), Positives = 46/89 (51%)
 Frame = +2

Query: 209 VFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPEDTHFVV 388
           + +V D     KFY + LGM++LR  E  E            +++  M+GY  E    V+
Sbjct: 152 MLRVGDLDRAIKFYEKALGMRLLRKIERPE-----------YKYTIGMMGYAEEYESIVL 200

Query: 389 ELTYNYGVTHYEQGNDFLGITVQSSESLK 475
           ELTYNY VT Y +GN +    +  ++++K
Sbjct: 201 ELTYNYDVTEYTKGNAYAQAQMMCTKAVK 229


>At1g11840.2 68414.m01362 lactoylglutathione lyase, putative /
           glyoxalase I, putative highly similar to putative
           lactoylglutathione lyase SP:Q39366 from [Brassica
           oleracea]
          Length = 283

 Score = 72.9 bits (171), Expect = 1e-13
 Identities = 48/131 (36%), Positives = 68/131 (51%), Gaps = 11/131 (8%)
 Frame = +2

Query: 194 RALHFVFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPED 373
           R LH V++V D   T +FY E+ GMK+LR  +  E            ++S   +G+GPE 
Sbjct: 17  RFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPE-----------EKYSNAFLGFGPET 65

Query: 374 THFVVELTYNYGVTHYEQGNDF--LGITVQSSESL---KRAQTNN-----WPIKEHNG-L 520
           ++FVVELTYNYGV+ Y+ G  F    I+ Q    L    RA+  N      P+K     +
Sbjct: 66  SNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVI 125

Query: 521 KYVEAPGGYKF 553
            +V+ P GY F
Sbjct: 126 AFVKDPDGYTF 136



 Score = 50.0 bits (114), Expect = 1e-06
 Identities = 30/92 (32%), Positives = 46/92 (50%)
 Frame = +2

Query: 209 VFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPEDTHFVV 388
           + +V D     KFY + LGM++LR  E  E            +++  M+GY  E    V+
Sbjct: 152 MLRVGDLDRAIKFYEKALGMRLLRKIERPE-----------YKYTIGMMGYAEEYESIVL 200

Query: 389 ELTYNYGVTHYEQGNDFLGITVQSSESLKRAQ 484
           ELTYNY VT Y +GN +  I + + +  K  +
Sbjct: 201 ELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 232


>At1g11840.1 68414.m01361 lactoylglutathione lyase, putative /
           glyoxalase I, putative highly similar to putative
           lactoylglutathione lyase SP:Q39366 from [Brassica
           oleracea]
          Length = 283

 Score = 72.9 bits (171), Expect = 1e-13
 Identities = 48/131 (36%), Positives = 68/131 (51%), Gaps = 11/131 (8%)
 Frame = +2

Query: 194 RALHFVFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPED 373
           R LH V++V D   T +FY E+ GMK+LR  +  E            ++S   +G+GPE 
Sbjct: 17  RFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPE-----------EKYSNAFLGFGPET 65

Query: 374 THFVVELTYNYGVTHYEQGNDF--LGITVQSSESL---KRAQTNN-----WPIKEHNG-L 520
           ++FVVELTYNYGV+ Y+ G  F    I+ Q    L    RA+  N      P+K     +
Sbjct: 66  SNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVI 125

Query: 521 KYVEAPGGYKF 553
            +V+ P GY F
Sbjct: 126 AFVKDPDGYTF 136



 Score = 50.0 bits (114), Expect = 1e-06
 Identities = 30/92 (32%), Positives = 46/92 (50%)
 Frame = +2

Query: 209 VFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANRWSKTMVGYGPEDTHFVV 388
           + +V D     KFY + LGM++LR  E  E            +++  M+GY  E    V+
Sbjct: 152 MLRVGDLDRAIKFYEKALGMRLLRKIERPE-----------YKYTIGMMGYAEEYESIVL 200

Query: 389 ELTYNYGVTHYEQGNDFLGITVQSSESLKRAQ 484
           ELTYNY VT Y +GN +  I + + +  K  +
Sbjct: 201 ELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 232


>At1g08110.2 68414.m00889 lactoylglutathione lyase, putative /
           glyoxalase I, putative similar to lactoylglutathione
           lyase SP:O04885 from [Brassica juncea]
          Length = 185

 Score = 31.9 bits (69), Expect = 0.30
 Identities = 27/94 (28%), Positives = 44/94 (46%), Gaps = 8/94 (8%)
 Frame = +2

Query: 209 VFKVADRTLTAKFYREILGMKVLRHEEFSE--------GCEAACNGPYANRWSKTMVGYG 364
           +F++ D   +  FY  +LGM +L+  +FSE        G E     P  +   +T+  +G
Sbjct: 32  MFRIKDPKASLDFYSRVLGMSLLKRLDFSEMKFSLYFLGYEDTTTAP-TDPTERTVWTFG 90

Query: 365 PEDTHFVVELTYNYGVTHYEQGNDFLGITVQSSE 466
              T   +ELT+N+G    E   +F G    +SE
Sbjct: 91  QPAT---IELTHNWGT---ESDPEFKGYHNGNSE 118


>At1g08110.1 68414.m00888 lactoylglutathione lyase, putative /
           glyoxalase I, putative similar to lactoylglutathione
           lyase SP:O04885 from [Brassica juncea]
          Length = 185

 Score = 31.9 bits (69), Expect = 0.30
 Identities = 27/94 (28%), Positives = 44/94 (46%), Gaps = 8/94 (8%)
 Frame = +2

Query: 209 VFKVADRTLTAKFYREILGMKVLRHEEFSE--------GCEAACNGPYANRWSKTMVGYG 364
           +F++ D   +  FY  +LGM +L+  +FSE        G E     P  +   +T+  +G
Sbjct: 32  MFRIKDPKASLDFYSRVLGMSLLKRLDFSEMKFSLYFLGYEDTTTAP-TDPTERTVWTFG 90

Query: 365 PEDTHFVVELTYNYGVTHYEQGNDFLGITVQSSE 466
              T   +ELT+N+G    E   +F G    +SE
Sbjct: 91  QPAT---IELTHNWGT---ESDPEFKGYHNGNSE 118


>At2g26680.1 68415.m03200 expressed protein similar to NLPE1
           (GI:13022100) [Rhizobium etli];
          Length = 319

 Score = 29.1 bits (62), Expect = 2.1
 Identities = 18/58 (31%), Positives = 27/58 (46%)
 Frame = +2

Query: 164 KLLNSKMVSGRALHFVFKVADRTLTAKFYREILGMKVLRHEEFSEGCEAACNGPYANR 337
           ++L+S   SG+    V  V      A F   ++G KVL  E   E  +  C+G + NR
Sbjct: 121 EVLDSMRASGKN-GIVVDVGANVGMASFAAAVMGFKVLAFEPVFENLQRICDGIWFNR 177


>At4g23090.1 68417.m03328 hypothetical protein 
          Length = 179

 Score = 27.9 bits (59), Expect = 4.9
 Identities = 16/38 (42%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = -1

Query: 377 VYLQGHIQPWFCSIGLHKVHCKLPR-ILH*TLHGGEPS 267
           +YL G  QP    +G+  VH   P  ILH +L G  PS
Sbjct: 114 LYLSGWFQPMLTDVGV-AVHSSRPNGILHVSLSGVSPS 150


>At1g31530.1 68414.m03867 endonuclease/exonuclease/phosphatase
           family protein contains similarity to nocturnin [Homo
           sapiens] GI:9885288; contains Pfam profile PF03372:
           Endonuclease/Exonuclease/phosphatase family
          Length = 283

 Score = 27.1 bits (57), Expect = 8.5
 Identities = 17/63 (26%), Positives = 29/63 (46%), Gaps = 2/63 (3%)
 Frame = -2

Query: 499 WPVVSLRPLQTLT*LYCDAKEIISLFV--MRYTIVVGQFNNKMCIFRAISNHGFAPSVCI 326
           W  + L  ++TL       KEIIS         I+ G FN+K  + + I++      + +
Sbjct: 152 WDDLKLAQVKTLMTELASFKEIISALTNCSPSVILAGDFNSKPYVHKYINSDNIPSDIDL 211

Query: 325 RSI 317
           RS+
Sbjct: 212 RSV 214


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,435,238
Number of Sequences: 28952
Number of extensions: 225396
Number of successful extensions: 547
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 533
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 539
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1062855648
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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