Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0409
(520 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 144 3e-35
At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 139 1e-33
At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 138 2e-33
At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 138 3e-33
At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 78 3e-15
At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 78 3e-15
At3g07770.1 68416.m00947 heat shock protein-related strong simil... 62 3e-10
At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 59 2e-09
At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 59 2e-09
At3g59050.1 68416.m06582 amine oxidase family protein similar to... 30 0.81
At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 29 2.5
At3g16480.1 68416.m02103 mitochondrial processing peptidase alph... 28 4.3
At4g05490.1 68417.m00830 F-box family protein (FBL22) contains s... 27 7.6
At1g51980.1 68414.m05863 mitochondrial processing peptidase alph... 27 7.6
>At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat
shock protein 83 (HSP83) nearly identical to SP|P27323
Heat shock protein 81-1 (HSP81-1) (Heat shock protein
83) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 705
Score = 144 bits (349), Expect = 3e-35
Identities = 72/106 (67%), Positives = 89/106 (83%)
Frame = -3
Query: 518 GYMAAKKHLEINPDHSIVETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHA 339
GYM++KK +EINPD+ I+E LR++AEADKNDK+VKDLV+LLYETALL+SGF+LDEP A
Sbjct: 604 GYMSSKKTMEINPDNGIMEELRKRAEADKNDKSVKDLVMLLYETALLTSGFSLDEPNTFA 663
Query: 338 SRIYRMIKLGLGIDEDEPIQVEEPASGDVPPLEGDADDASRMEEVD 201
+RI+RM+KLGL IDEDE VEE GD+P LE DA + S+MEEVD
Sbjct: 664 ARIHRMLKLGLSIDEDE--NVEE--DGDMPELEEDAAEESKMEEVD 705
>At5g56010.1 68418.m06989 heat shock protein, putative strong
similarity to SP|P55737 Heat shock protein 81-2
(HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00183: Hsp90 protein
Length = 699
Score = 139 bits (336), Expect = 1e-33
Identities = 66/107 (61%), Positives = 90/107 (84%), Gaps = 1/107 (0%)
Frame = -3
Query: 518 GYMAAKKHLEINPDHSIVETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHA 339
GYM++KK +EINP++SI++ LR++A+ADKNDK+VKDLV+LL+ETALL+SGF+LDEP
Sbjct: 597 GYMSSKKTMEINPENSIMDELRKRADADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFG 656
Query: 338 SRIYRMIKLGLGIDEDEPIQVEEPASGDVPPLEGDAD-DASRMEEVD 201
SRI+RM+KLGL ID+D+ ++ A D+PPLE DAD + S+MEEVD
Sbjct: 657 SRIHRMLKLGLSIDDDDVVE----ADADMPPLEDDADAEGSKMEEVD 699
>At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly
identical to heat shock protein hsp81.4 [Arabidopsis
thaliana] GI:1906828; contains Pfam profiles PF02518:
ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like
domain protein, PF00183: Hsp90 protein
Length = 699
Score = 138 bits (335), Expect = 2e-33
Identities = 66/107 (61%), Positives = 90/107 (84%), Gaps = 1/107 (0%)
Frame = -3
Query: 518 GYMAAKKHLEINPDHSIVETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHA 339
GYM++KK +EINP++SI++ LR++AEADKNDK+VKDLV+LL+ETALL+SGF+LDEP
Sbjct: 597 GYMSSKKTMEINPENSIMDELRKRAEADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFG 656
Query: 338 SRIYRMIKLGLGIDEDEPIQVEEPASGDVPPLEGDAD-DASRMEEVD 201
SRI+RM+KLGL I+ED+ ++ A ++PPLE DAD + S+MEEVD
Sbjct: 657 SRIHRMLKLGLSIEEDDAVE----ADAEMPPLEDDADAEGSKMEEVD 699
>At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly
identical to SP|P55737 Heat shock protein 81-2 (HSP81-2)
{Arabidopsis thaliana}
Length = 699
Score = 138 bits (333), Expect = 3e-33
Identities = 65/107 (60%), Positives = 90/107 (84%), Gaps = 1/107 (0%)
Frame = -3
Query: 518 GYMAAKKHLEINPDHSIVETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHA 339
GYM++KK +EINP++SI++ LR++A+ADKNDK+VKDLV+LL+ETALL+SGF+LDEP
Sbjct: 597 GYMSSKKTMEINPENSIMDELRKRADADKNDKSVKDLVLLLFETALLTSGFSLDEPNTFG 656
Query: 338 SRIYRMIKLGLGIDEDEPIQVEEPASGDVPPLEGDAD-DASRMEEVD 201
SRI+RM+KLGL ID+D+ ++ A ++PPLE DAD + S+MEEVD
Sbjct: 657 SRIHRMLKLGLSIDDDDAVE----ADAEMPPLEDDADAEGSKMEEVD 699
>At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 78.2 bits (184), Expect = 3e-15
Identities = 40/99 (40%), Positives = 60/99 (60%), Gaps = 5/99 (5%)
Frame = -3
Query: 515 YMAAKKHLEINPDHSIVETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHAS 336
YM K+ LEINP H I++ L+ + +D D++VK+ L+Y+TAL+ SGF L +P+ A+
Sbjct: 701 YMRGKRVLEINPRHPIIKELKDRIASDPEDESVKETAQLMYQTALIESGFILTDPKDFAA 760
Query: 335 RIYRMIKLGLGID----EDEPIQ-VEEPASGDVPPLEGD 234
RIY +K GL I DE I+ EEP + + + D
Sbjct: 761 RIYNSVKSGLNISPDAVADEEIEAAEEPETSEATETKSD 799
>At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation
protein, putative nearly identical to SHEPHERD
[Arabidopsis thaliana] GI:19570872; contains Pfam
profiles PF02518: ATPase, histidine kinase-, DNA gyrase
B-, and HSP90-like domain protein, PF00183: Hsp90
protein
Length = 823
Score = 78.2 bits (184), Expect = 3e-15
Identities = 40/99 (40%), Positives = 60/99 (60%), Gaps = 5/99 (5%)
Frame = -3
Query: 515 YMAAKKHLEINPDHSIVETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHAS 336
YM K+ LEINP H I++ L+ + +D D++VK+ L+Y+TAL+ SGF L +P+ A+
Sbjct: 701 YMRGKRVLEINPRHPIIKELKDRIASDPEDESVKETAQLMYQTALIESGFILTDPKDFAA 760
Query: 335 RIYRMIKLGLGID----EDEPIQ-VEEPASGDVPPLEGD 234
RIY +K GL I DE I+ EEP + + + D
Sbjct: 761 RIYNSVKSGLNISPDAVADEEIEAAEEPETSEATETKSD 799
>At3g07770.1 68416.m00947 heat shock protein-related strong
similarity to heat-shock protein [Secale cereale]
GI:556673; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 803
Score = 61.7 bits (143), Expect = 3e-10
Identities = 33/98 (33%), Positives = 51/98 (52%), Gaps = 4/98 (4%)
Frame = -3
Query: 515 YMAAKKHLEINPDHSIVETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHAS 336
YM ++ EINPDHSI++ + ++ ND+ + L+Y+ AL+SSGFT D P
Sbjct: 695 YMKGRRVFEINPDHSIIKNINAAYNSNPNDEDAMRAIDLMYDAALVSSGFTPDNPAELGG 754
Query: 335 RIYRMIKLGL----GIDEDEPIQVEEPASGDVPPLEGD 234
+IY M+ + L E +P Q + S D E +
Sbjct: 755 KIYEMMDVALSGKWSSPEVQPQQQQMAHSHDAETFEAE 792
>At2g04030.2 68415.m00372 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 777
Score = 58.8 bits (136), Expect = 2e-09
Identities = 27/71 (38%), Positives = 42/71 (59%)
Frame = -3
Query: 515 YMAAKKHLEINPDHSIVETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHAS 336
+M ++ LEINPDH I++ L + +V LLY+TA++SSGFT D P +
Sbjct: 665 FMRGRRILEINPDHPIIKDLNAACKNAPESTEATRVVDLLYDTAIISSGFTPDSPAELGN 724
Query: 335 RIYRMIKLGLG 303
+IY M+ + +G
Sbjct: 725 KIYEMMAMAVG 735
>At2g04030.1 68415.m00371 heat shock protein, putative strong
similarity to heat shock protein [Arabidopsis thaliana]
GI:1906830; contains Pfam profiles PF02518: ATPase,
histidine kinase-, DNA gyrase B-, and HSP90-like domain
protein, PF00183: Hsp90 protein
Length = 780
Score = 58.8 bits (136), Expect = 2e-09
Identities = 27/71 (38%), Positives = 42/71 (59%)
Frame = -3
Query: 515 YMAAKKHLEINPDHSIVETLRQKAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHAS 336
+M ++ LEINPDH I++ L + +V LLY+TA++SSGFT D P +
Sbjct: 668 FMRGRRILEINPDHPIIKDLNAACKNAPESTEATRVVDLLYDTAIISSGFTPDSPAELGN 727
Query: 335 RIYRMIKLGLG 303
+IY M+ + +G
Sbjct: 728 KIYEMMAMAVG 738
>At3g59050.1 68416.m06582 amine oxidase family protein similar to
polyamine oxidase (EC 1.5.3.11) precursor - Zea mays
[SP|O64411]; contains Pfam profile PF01593 amine
oxidase, flavin-containing
Length = 488
Score = 30.3 bits (65), Expect = 0.81
Identities = 20/72 (27%), Positives = 33/72 (45%), Gaps = 4/72 (5%)
Frame = -3
Query: 500 KHLEINPDHSIVETLRQ----KAEADKNDKAVKDLVILLYETALLSSGFTLDEPQVHASR 333
K L+I H I + R+ K +K D V D ++ +L SG EP++ +
Sbjct: 237 KGLDIRLSHRITKISRRYSGVKVTTEKGDTFVADAAVIALPLGVLKSGMITFEPKLPQWK 296
Query: 332 IYRMIKLGLGID 297
+ LG+GI+
Sbjct: 297 QEAINDLGVGIE 308
>At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative
similar to delta-8 sphingolipid desaturase GI:3819708
from [Brassica napus]
Length = 449
Score = 28.7 bits (61), Expect = 2.5
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = +3
Query: 156 TNHYDMVPTVKVEILINLLHARCIIGITLQW 248
+ HY + T LI LL C+ GI++ W
Sbjct: 162 SGHYTVTSTKPCNKLIQLLSGNCLTGISIAW 192
>At3g16480.1 68416.m02103 mitochondrial processing peptidase alpha
subunit, putative similar to mitochondrial processing
peptidase alpha subunit, mitochondrial precursor,
Alpha-MPP (Ubiquinol-cytochrome C reductase subunit II)
[Potato] SWISS-PROT:P29677
Length = 499
Score = 27.9 bits (59), Expect = 4.3
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = -3
Query: 326 RMIKLGLGIDEDEPIQVEEPASGDVPPLEGDADDASR 216
RM+ G+D +E ++V EP D+P + A+ S+
Sbjct: 253 RMVLAASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQ 289
>At4g05490.1 68417.m00830 F-box family protein (FBL22) contains
similarity to N7 protein GI:3273101 from [Medicago
truncatula]
Length = 307
Score = 27.1 bits (57), Expect = 7.6
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = -1
Query: 304 VLMKMSRFRLKSLLLEMCRHWRVMPMMHLAWRRL 203
+L+++S + ++CR WR + L WRR+
Sbjct: 38 ILLRLSPLEILENARKVCRSWRRVSKDPLIWRRI 71
>At1g51980.1 68414.m05863 mitochondrial processing peptidase alpha
subunit, putative similar to mitochondrial processing
peptidase alpha subunit, mitochondrial precursor,
Alpha-MPP (Ubiquinol-cytochrome C reductase subunit II)
[Potato] SWISS-PROT:P29677
Length = 503
Score = 27.1 bits (57), Expect = 7.6
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = -3
Query: 374 SGFTLDEPQVHASRIYRMIKLGLGIDEDEPIQVEEPASGDVP 249
+G L+E RM+ G++ +E ++V EP + D+P
Sbjct: 241 NGELLEEFMTENFTAARMVLAASGVEHEELLKVAEPLTSDLP 282
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,455,268
Number of Sequences: 28952
Number of extensions: 190067
Number of successful extensions: 447
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 427
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 443
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 947539968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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