Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0399
(395 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q7QAW0 Cluster: ENSANGP00000011026; n=2; Culicidae|Rep:... 98 5e-20
UniRef50_UPI0000E1FE2F Cluster: PREDICTED: similar to Nitrilase ... 94 8e-19
UniRef50_Q8WUF0 Cluster: Nitrilase family member 2; n=28; cellul... 94 8e-19
UniRef50_Q7QKM8 Cluster: ENSANGP00000017134; n=5; Culicidae|Rep:... 88 5e-17
UniRef50_Q6TGW8 Cluster: Nit protein 2; n=22; Fungi/Metazoa grou... 86 3e-16
UniRef50_Q54JM9 Cluster: Putative uncharacterized protein; n=1; ... 83 2e-15
UniRef50_Q8RUF8 Cluster: AT5g12040/F14F18_210; n=9; Magnoliophyt... 78 7e-14
UniRef50_Q5DC61 Cluster: SJCHGC06938 protein; n=1; Schistosoma j... 77 1e-13
UniRef50_A2XD42 Cluster: Putative uncharacterized protein; n=2; ... 75 7e-13
UniRef50_Q4Q8W4 Cluster: Nitrilase, putative; n=6; Trypanosomati... 75 7e-13
UniRef50_A7GE66 Cluster: Hydrolase, carbon-nitrogen family; n=13... 68 8e-11
UniRef50_Q2NHR0 Cluster: Predicted amidohydrolase; n=1; Methanos... 66 2e-10
UniRef50_Q5KJU9 Cluster: Hydrolase, putative; n=1; Filobasidiell... 61 9e-09
UniRef50_Q0AX54 Cluster: N-carbamoyl-D-amino acid amidohydrolase... 60 2e-08
UniRef50_Q2TYD8 Cluster: Carbon-nitrogen hydrolase; n=1; Aspergi... 53 2e-06
UniRef50_A7I5W9 Cluster: Porphyromonas-type peptidyl-arginine de... 47 1e-04
UniRef50_Q1IQA8 Cluster: Nitrilase/cyanide hydratase and apolipo... 47 2e-04
UniRef50_Q8NLZ3 Cluster: Predicted amidohydrolase; n=3; Coryneba... 46 2e-04
UniRef50_Q4P4D1 Cluster: Putative uncharacterized protein; n=1; ... 46 3e-04
UniRef50_A3ZLM3 Cluster: Putative nitrilase; n=1; Blastopirellul... 46 4e-04
UniRef50_Q1PXD4 Cluster: Similar to N-carbamoyl-D-amino acid hyd... 45 6e-04
UniRef50_Q82UY9 Cluster: Carbon-nitrogen hydrolase; n=50; Proteo... 44 8e-04
UniRef50_Q5L031 Cluster: Beta-alanine synthase; n=19; Bacteria|R... 44 8e-04
UniRef50_Q9UYV8 Cluster: Beta ureidopropionase; n=4; Thermococca... 44 0.001
UniRef50_Q97RA3 Cluster: Carbon-nitrogen hydrolase family protei... 43 0.002
UniRef50_Q46AW4 Cluster: Putative amidohydrolase; n=1; Methanosa... 43 0.002
UniRef50_Q8FM85 Cluster: Putative uncharacterized protein; n=2; ... 42 0.004
UniRef50_Q606Z9 Cluster: Hydrolase, carbon-nitrogen family; n=38... 42 0.004
UniRef50_A0BR54 Cluster: Chromosome undetermined scaffold_122, w... 42 0.006
UniRef50_Q8Y8V0 Cluster: Lmo0792 protein; n=12; Listeria|Rep: Lm... 41 0.008
UniRef50_A0TTW8 Cluster: Nitrilase/cyanide hydratase and apolipo... 41 0.010
UniRef50_Q6SHH5 Cluster: Carbon-nitrogen hydrolase family protei... 40 0.013
UniRef50_Q6N4F1 Cluster: Possible amidohydrolase; n=2; Rhodopseu... 40 0.017
UniRef50_Q5C342 Cluster: SJCHGC04680 protein; n=1; Schistosoma j... 40 0.017
UniRef50_Q92DM8 Cluster: Lin0785 protein; n=5; Bacteria|Rep: Lin... 40 0.023
UniRef50_Q69NN4 Cluster: Putative uncharacterized protein OSJNBb... 40 0.023
UniRef50_A5V962 Cluster: Nitrilase/cyanide hydratase and apolipo... 39 0.031
UniRef50_A5GU42 Cluster: Nitrilase-related protein; n=1; Synecho... 39 0.031
UniRef50_A0JSW0 Cluster: Nitrilase/cyanide hydratase and apolipo... 39 0.040
UniRef50_O76463 Cluster: Nitrilase and fragile histidine triad f... 39 0.040
UniRef50_Q0F1V1 Cluster: Hydrolase, carbon-nitrogen family prote... 38 0.053
UniRef50_A4J4S3 Cluster: Nitrilase/cyanide hydratase and apolipo... 38 0.053
UniRef50_Q9ZMC7 Cluster: Putative; n=6; Campylobacterales|Rep: P... 38 0.071
UniRef50_Q1AWK1 Cluster: Nitrilase/cyanide hydratase and apolipo... 37 0.12
UniRef50_Q4JAH2 Cluster: Conserved protein; n=4; Sulfolobaceae|R... 36 0.22
UniRef50_Q74H63 Cluster: Hydrolase, carbon-nitrogen family; n=8;... 36 0.28
UniRef50_Q9ABL5 Cluster: Hydrolase, carbon-nitrogen family; n=13... 36 0.38
UniRef50_Q6JHR5 Cluster: Aliphatic amidase; n=1; Saccharopolyspo... 36 0.38
UniRef50_Q12ZA5 Cluster: Nitrilase/cyanide hydratase and apolipo... 36 0.38
UniRef50_Q60BT4 Cluster: Hydrolase, carbon-nitrogen family; n=15... 35 0.50
UniRef50_Q1JW05 Cluster: Nitrilase/cyanide hydratase and apolipo... 35 0.50
UniRef50_Q8TLM7 Cluster: Carbon-nitrogen hydrolase; n=2; Methano... 35 0.50
UniRef50_Q6L0F7 Cluster: Carbon-nitrogen hydrolase family; n=2; ... 35 0.50
UniRef50_A6DN63 Cluster: Nitrilase/cyanide hydratase and apolipo... 35 0.66
UniRef50_A4B9A7 Cluster: Probable hydratase; n=2; Bacteria|Rep: ... 34 0.87
UniRef50_A0RYH6 Cluster: Amidohydrolase; n=1; Cenarchaeum symbio... 34 0.87
UniRef50_P55175 Cluster: UPF0012 hydrolase sll0601; n=40; Cyanob... 34 0.87
UniRef50_Q0AM99 Cluster: Peptidase S8 and S53, subtilisin, kexin... 34 1.1
UniRef50_A0BLB1 Cluster: Chromosome undetermined scaffold_114, w... 34 1.1
UniRef50_Q972L1 Cluster: 281aa long hypothetical beta-ureidoprop... 34 1.1
UniRef50_O76464 Cluster: Nitrilase and fragile histidine triad f... 34 1.1
UniRef50_Q2S196 Cluster: Hydrolase, carbon-nitrogen family; n=1;... 33 1.5
UniRef50_Q1GTC5 Cluster: Nitrilase/cyanide hydratase and apolipo... 33 1.5
UniRef50_O30121 Cluster: Putative uncharacterized protein; n=1; ... 33 1.5
UniRef50_Q6RWN7 Cluster: Nitrilase; n=21; root|Rep: Nitrilase - ... 33 2.0
UniRef50_A4U2A6 Cluster: Nitrilase/cyanide hydratase and apolipo... 33 2.0
UniRef50_Q183H2 Cluster: Putative carbon-nitrogen hydrolase; n=2... 32 3.5
UniRef50_Q4Q7M9 Cluster: Putative uncharacterized protein; n=6; ... 32 3.5
UniRef50_Q88EJ9 Cluster: Carbon-nitrogen hydrolase family protei... 32 4.6
UniRef50_Q7UWX1 Cluster: Beta-alanine synthetase; n=1; Pirellula... 32 4.6
UniRef50_Q025F3 Cluster: Nitrilase/cyanide hydratase and apolipo... 32 4.6
UniRef50_Q971J8 Cluster: Putative uncharacterized protein STS165... 32 4.6
UniRef50_A4SNH5 Cluster: Amidohydrolase family protein; n=2; Pro... 31 6.1
UniRef50_A4ALG5 Cluster: Putative hydrolase; n=2; Actinobacteria... 31 6.1
UniRef50_A2ICY3 Cluster: Cyanide hydratase; n=23; Gammaproteobac... 31 6.1
UniRef50_A0J1U1 Cluster: Nitrilase/cyanide hydratase and apolipo... 31 6.1
UniRef50_A7QG85 Cluster: Chromosome undetermined scaffold_91, wh... 31 6.1
UniRef50_Q0W654 Cluster: Putative amidohydrolase; n=1; unculture... 31 6.1
UniRef50_Q1YU23 Cluster: Hydrolase, carbon-nitrogen family prote... 31 8.1
UniRef50_A6Q8M5 Cluster: Carbon-nitrogen hydrolase family protei... 31 8.1
UniRef50_A4M5M1 Cluster: Nitrilase/cyanide hydratase and apolipo... 31 8.1
UniRef50_A1IEG0 Cluster: Amidohydrolase-like precursor; n=1; Can... 31 8.1
UniRef50_A0GGV1 Cluster: Nitrilase/cyanide hydratase and apolipo... 31 8.1
UniRef50_A1D103 Cluster: Hydrolase, carbon-nitrogen family prote... 31 8.1
>UniRef50_Q7QAW0 Cluster: ENSANGP00000011026; n=2; Culicidae|Rep:
ENSANGP00000011026 - Anopheles gambiae str. PEST
Length = 278
Score = 98.3 bits (234), Expect = 5e-20
Identities = 47/102 (46%), Positives = 63/102 (61%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPSG 265
++AL+QL P K + IA A+ +I AK++GA+L+ LPECFNSPY T F +AEE+P G
Sbjct: 7 RVALVQLYGRPTKQECIANAISQIRQAKDRGARLIILPECFNSPYSTAEFGRHAEEIPRG 66
Query: 266 ETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWDDTXE 391
ETS+AL+K PER +LYNTC V+ E
Sbjct: 67 ETSQALAKVAAELGVYLVGGTYPEREGTRLYNTCPVFGPKGE 108
>UniRef50_UPI0000E1FE2F Cluster: PREDICTED: similar to Nitrilase
family, member 2; n=2; Coelomata|Rep: PREDICTED: similar
to Nitrilase family, member 2 - Pan troglodytes
Length = 411
Score = 94.3 bits (224), Expect = 8e-19
Identities = 46/98 (46%), Positives = 62/98 (63%)
Frame = +2
Query: 83 FKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
F++ALIQL + KS N+ +A I A +GA++V+LPECFNSPYGTKYF EYAE++P
Sbjct: 139 FRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGTKYFPEYAEKIP- 197
Query: 263 GETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVW 376
GE+++ L + PE KLYNTC V+
Sbjct: 198 GESTQKLCEVAKECSIYLIGGSIPEEDAGKLYNTCAVF 235
>UniRef50_Q8WUF0 Cluster: Nitrilase family member 2; n=28; cellular
organisms|Rep: Nitrilase family member 2 - Homo sapiens
(Human)
Length = 276
Score = 94.3 bits (224), Expect = 8e-19
Identities = 46/98 (46%), Positives = 62/98 (63%)
Frame = +2
Query: 83 FKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
F++ALIQL + KS N+ +A I A +GA++V+LPECFNSPYG KYF EYAE++P
Sbjct: 4 FRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIP- 62
Query: 263 GETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVW 376
GE+++ LS+ PE KLYNTC V+
Sbjct: 63 GESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVF 100
>UniRef50_Q7QKM8 Cluster: ENSANGP00000017134; n=5; Culicidae|Rep:
ENSANGP00000017134 - Anopheles gambiae str. PEST
Length = 281
Score = 88.2 bits (209), Expect = 5e-17
Identities = 48/102 (47%), Positives = 56/102 (54%), Gaps = 1/102 (0%)
Frame = +2
Query: 74 KRGFKIALIQLSVGPDKSKNIAQAVKEIHLAK-EKGAQLVALPECFNSPYGTKYFDEYAE 250
K KIALIQL V K KN+ A+ I +AK EK A +V LPECFN+PY AE
Sbjct: 4 KMTIKIALIQLRVVDSKEKNLKNAIDLIRIAKKEKDANVVVLPECFNAPYTADTLLNVAE 63
Query: 251 EVPSGETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVW 376
E+P+GET RALS E +LYNTCTVW
Sbjct: 64 EIPTGETCRALSNAARDFGVHVVGGSIVESCSGRLYNTCTVW 105
>UniRef50_Q6TGW8 Cluster: Nit protein 2; n=22; Fungi/Metazoa
group|Rep: Nit protein 2 - Danio rerio (Zebrafish)
(Brachydanio rerio)
Length = 277
Score = 85.8 bits (203), Expect = 3e-16
Identities = 42/98 (42%), Positives = 62/98 (63%)
Frame = +2
Query: 83 FKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
F++A++QL V K+ N+ +A + A +GA++V LPECFNSPYGT +F EYAE++P
Sbjct: 4 FRLAVVQLHVSKIKADNLGRAQTLVTEAAGQGAKVVVLPECFNSPYGTGFFKEYAEKIP- 62
Query: 263 GETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVW 376
GE+++ LS+ PE KLYNTC+V+
Sbjct: 63 GESTQVLSETAKKCGIYLVGGSIPEEDGGKLYNTCSVF 100
>UniRef50_Q54JM9 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 328
Score = 83.0 bits (196), Expect = 2e-15
Identities = 43/105 (40%), Positives = 61/105 (58%), Gaps = 2/105 (1%)
Frame = +2
Query: 83 FKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
FK A IQL G +K +N+ A+K I A + GA+L++LPECFNSPY T F++Y+ E
Sbjct: 53 FKFAGIQLLCGDNKEENVQNAIKHIDEAAKNGAKLISLPECFNSPYSTSTFEKYS-ETED 111
Query: 263 GETSRALSKXXXXXXXXXXXXXXPE--RYEKKLYNTCTVWDDTXE 391
GET + LS+ PE + K+YNTC +++D E
Sbjct: 112 GETVKKLSEAAKRNQIFLVGGSIPEIDKATGKIYNTCFIFNDKGE 156
>UniRef50_Q8RUF8 Cluster: AT5g12040/F14F18_210; n=9;
Magnoliophyta|Rep: AT5g12040/F14F18_210 - Arabidopsis
thaliana (Mouse-ear cress)
Length = 369
Score = 77.8 bits (183), Expect = 7e-14
Identities = 45/107 (42%), Positives = 56/107 (52%), Gaps = 4/107 (3%)
Frame = +2
Query: 83 FKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
F I L QLSV DK +NI+ A K I A KGA+LV LPE +NSPY F YAEE+ +
Sbjct: 88 FNIGLCQLSVTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPYSNDSFPVYAEEIDA 147
Query: 263 G----ETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWDDTXE 391
G ++ LS+ PER +LYNTC V+ E
Sbjct: 148 GGDASPSTAMLSEVSKRLKITIIGGSIPERVGDRLYNTCCVFGSDGE 194
>UniRef50_Q5DC61 Cluster: SJCHGC06938 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC06938 protein - Schistosoma
japonicum (Blood fluke)
Length = 290
Score = 77.0 bits (181), Expect = 1e-13
Identities = 42/104 (40%), Positives = 56/104 (53%), Gaps = 2/104 (1%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLA-KEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
++AL+Q+ VG DK+ N+ +A I A E AQLV LPECF SP G KYF+ YAE VP+
Sbjct: 3 RLALVQMFVGTDKAANLKRASDLISRAVSEHSAQLVCLPECFTSPIGAKYFEPYAEPVPN 62
Query: 263 GETSRALSKXXXXXXXXXXXXXXPER-YEKKLYNTCTVWDDTXE 391
G + LS ER + K+YN C ++ E
Sbjct: 63 GPACQMLSNAAKSHKIWLVGGSISERGSDGKIYNCCATYNPDGE 106
>UniRef50_A2XD42 Cluster: Putative uncharacterized protein; n=2;
Oryza sativa|Rep: Putative uncharacterized protein -
Oryza sativa subsp. indica (Rice)
Length = 349
Score = 74.5 bits (175), Expect = 7e-13
Identities = 43/107 (40%), Positives = 57/107 (53%), Gaps = 4/107 (3%)
Frame = +2
Query: 83 FKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
FK+AL QLSV DK++NIA+A + I A GA+LV LPE +N PY F EYAE++ +
Sbjct: 46 FKVALCQLSVTADKARNIARAREAIEAAAAGGAKLVLLPEIWNGPYSNDSFPEYAEDIEA 105
Query: 263 G----ETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWDDTXE 391
G + +S+ ER KLYNTC V+ E
Sbjct: 106 GGDAAPSFSMMSEVARSLQITLVGGSISERSGNKLYNTCCVFGSDGE 152
>UniRef50_Q4Q8W4 Cluster: Nitrilase, putative; n=6;
Trypanosomatidae|Rep: Nitrilase, putative - Leishmania
major
Length = 279
Score = 74.5 bits (175), Expect = 7e-13
Identities = 39/94 (41%), Positives = 57/94 (60%), Gaps = 2/94 (2%)
Frame = +2
Query: 89 IALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEV-PSG 265
+ L Q++V +K+ NI +AV I A ++G++L LPECFN PYGTKYFDEY+E + P
Sbjct: 7 VTLCQMAVTREKAANIKKAVTMITEAAKRGSKLAVLPECFNCPYGTKYFDEYSEALAPGN 66
Query: 266 ETSRALSKXXXXXXXXXXXXXXPER-YEKKLYNT 364
ET A+S+ PE+ + KL+N+
Sbjct: 67 ETFDAMSQCAKANSIWIVAGSIPEKSADGKLFNS 100
>UniRef50_A7GE66 Cluster: Hydrolase, carbon-nitrogen family; n=13;
cellular organisms|Rep: Hydrolase, carbon-nitrogen
family - Clostridium botulinum (strain Langeland / NCTC
10281 / Type F)
Length = 278
Score = 67.7 bits (158), Expect = 8e-11
Identities = 38/102 (37%), Positives = 52/102 (50%), Gaps = 3/102 (2%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAE---EV 256
KIAL Q+ V +K KNI +A++ + AK++ + LPE FN PY K F Y E E
Sbjct: 5 KIALCQMQVQKEKKKNIKKAIEMLTKAKKENCNIAVLPEMFNCPYENKCFKPYGEIINEE 64
Query: 257 PSGETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWDD 382
GET +A+ K PE K+YNT V+D+
Sbjct: 65 NGGETVKAIKKAAKDLELYIVAGSIPEIEGDKIYNTSMVFDN 106
>UniRef50_Q2NHR0 Cluster: Predicted amidohydrolase; n=1;
Methanosphaera stadtmanae DSM 3091|Rep: Predicted
amidohydrolase - Methanosphaera stadtmanae (strain DSM
3091)
Length = 274
Score = 66.5 bits (155), Expect = 2e-10
Identities = 34/96 (35%), Positives = 48/96 (50%)
Frame = +2
Query: 77 RGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEV 256
+ FKIA Q++V +K NI A++ I A GA+L+ LPE FN+PY F EY EE
Sbjct: 2 KDFKIATCQMNVVDNKDTNIEHAIQLIKKASSNGAKLITLPEMFNTPYDNSKFIEYCEEE 61
Query: 257 PSGETSRALSKXXXXXXXXXXXXXXPERYEKKLYNT 364
+ +T ++ PE+ LYNT
Sbjct: 62 TTSKTLNSMQDIAREENIYLQSGSIPEKESNHLYNT 97
>UniRef50_Q5KJU9 Cluster: Hydrolase, putative; n=1; Filobasidiella
neoformans|Rep: Hydrolase, putative - Cryptococcus
neoformans (Filobasidiella neoformans)
Length = 301
Score = 60.9 bits (141), Expect = 9e-09
Identities = 43/117 (36%), Positives = 58/117 (49%), Gaps = 18/117 (15%)
Frame = +2
Query: 83 FKIALIQLS-VGPDKSKNIAQAVKEIHLAKEKGA--QLVALPECFNSPYGTKYFDEYAEE 253
F++AL+QL + K+ NI+ A K + A QL+ LPE +NSPY F EY+E+
Sbjct: 9 FRLALLQLGGLTASKASNISIAAKAVTSAAASSPKPQLIVLPEIWNSPYAVSSFREYSEK 68
Query: 254 VP-------------SGETSRALSKXXXXXXXXXXXXXXPERYEK--KLYNTCTVWD 379
VP GET +AL + PER EK +YNTCTV+D
Sbjct: 69 VPEVGSKWKSLKEGEEGETIKALREMARSSGCWLIGGSIPERDEKTDNIYNTCTVYD 125
>UniRef50_Q0AX54 Cluster: N-carbamoyl-D-amino acid amidohydrolase;
n=1; Syntrophomonas wolfei subsp. wolfei str.
Goettingen|Rep: N-carbamoyl-D-amino acid amidohydrolase
- Syntrophomonas wolfei subsp. wolfei (strain
Goettingen)
Length = 283
Score = 60.1 bits (139), Expect = 2e-08
Identities = 31/107 (28%), Positives = 51/107 (47%)
Frame = +2
Query: 71 LKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAE 250
+ + +++ Q+ G DK +N+ +A + I A +GA++V LPE FNSPY + F YAE
Sbjct: 1 MDKTLSLSICQMKTGNDKDENLKKAGEMIAAAAGEGAEMVVLPEVFNSPYQAELFPRYAE 60
Query: 251 EVPSGETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWDDTXE 391
P T + + K+YN+ V+D+ E
Sbjct: 61 PFPGPSTDFLAAAACKHGLCIVGGSIIERDSQGKIYNSSFVFDERGE 107
>UniRef50_Q2TYD8 Cluster: Carbon-nitrogen hydrolase; n=1;
Aspergillus oryzae|Rep: Carbon-nitrogen hydrolase -
Aspergillus oryzae
Length = 244
Score = 52.8 bits (121), Expect = 2e-06
Identities = 23/43 (53%), Positives = 29/43 (67%)
Frame = +2
Query: 122 KSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAE 250
K +N+A A +++ A KGA L+ LPECFNSPY F EYAE
Sbjct: 11 KVQNLANATQKVLQAASKGASLIILPECFNSPYSATKFREYAE 53
>UniRef50_A7I5W9 Cluster: Porphyromonas-type peptidyl-arginine
deiminase; n=1; Candidatus Methanoregula boonei 6A8|Rep:
Porphyromonas-type peptidyl-arginine deiminase -
Methanoregula boonei (strain 6A8)
Length = 640
Score = 47.2 bits (107), Expect = 1e-04
Identities = 19/44 (43%), Positives = 29/44 (65%)
Frame = +2
Query: 89 IALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY 220
IALIQ+ +GPD +N+ +A + + A + GAQ + LPE F + Y
Sbjct: 8 IALIQMEIGPDPDRNLNEARERVEKAAQNGAQFICLPELFRTRY 51
>UniRef50_Q1IQA8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=52; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Acidobacteria bacterium (strain
Ellin345)
Length = 303
Score = 46.8 bits (106), Expect = 2e-04
Identities = 25/69 (36%), Positives = 39/69 (56%), Gaps = 4/69 (5%)
Frame = +2
Query: 83 FKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFD----EYAE 250
F I LIQ+S GP +N+A+A+ + A ++GA ++ LPE F + Y + D E AE
Sbjct: 6 FTIGLIQMSCGPVPEENMAKALDRVRDAAKQGATVICLPELFQTQYFCQREDTALFELAE 65
Query: 251 EVPSGETSR 277
+P T +
Sbjct: 66 SIPGPATKK 74
>UniRef50_Q8NLZ3 Cluster: Predicted amidohydrolase; n=3;
Corynebacterium|Rep: Predicted amidohydrolase -
Corynebacterium glutamicum (Brevibacterium flavum)
Length = 266
Score = 46.4 bits (105), Expect = 2e-04
Identities = 26/68 (38%), Positives = 37/68 (54%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPSG 265
+IAL+Q+S DK N A A E+GA+++ PE + +GT D AEE+ G
Sbjct: 2 RIALLQISTNSDKMDNFALLRDAAEKAAEQGARVLVFPEATSQSFGTGRLDTQAEEL-DG 60
Query: 266 ETSRALSK 289
E S A+ K
Sbjct: 61 EFSTAVRK 68
>UniRef50_Q4P4D1 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 373
Score = 46.0 bits (104), Expect = 3e-04
Identities = 18/22 (81%), Positives = 19/22 (86%)
Frame = +2
Query: 185 LVALPECFNSPYGTKYFDEYAE 250
+V LPECFNSPYG KYF EYAE
Sbjct: 58 MVVLPECFNSPYGVKYFAEYAE 79
>UniRef50_A3ZLM3 Cluster: Putative nitrilase; n=1; Blastopirellula
marina DSM 3645|Rep: Putative nitrilase -
Blastopirellula marina DSM 3645
Length = 258
Score = 45.6 bits (103), Expect = 4e-04
Identities = 31/94 (32%), Positives = 46/94 (48%), Gaps = 2/94 (2%)
Frame = +2
Query: 104 LSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPSGETSRAL 283
++ G DK N+ A + I A E+GAQLV LPE FN + E+AE + SG T+ +
Sbjct: 1 MNAGEDKELNLQTAERLIAQAAERGAQLVVLPELFNYLGRLENLVEHAETI-SGPTAVRM 59
Query: 284 SKXXXXXXXXXXXXXXPER--YEKKLYNTCTVWD 379
K ER E +++NT ++D
Sbjct: 60 RKAALKHQIYLVAGSFAERSETESRVFNTSLIFD 93
>UniRef50_Q1PXD4 Cluster: Similar to N-carbamoyl-D-amino acid
hydrolase; n=1; Candidatus Kuenenia stuttgartiensis|Rep:
Similar to N-carbamoyl-D-amino acid hydrolase -
Candidatus Kuenenia stuttgartiensis
Length = 277
Score = 44.8 bits (101), Expect = 6e-04
Identities = 33/101 (32%), Positives = 49/101 (48%), Gaps = 2/101 (1%)
Frame = +2
Query: 89 IALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPSGE 268
IA IQ+ D++KN+ A + A +KGA+L+ALPE F+ + +AEE +GE
Sbjct: 6 IAAIQMCSVHDRNKNLNTARVLMEKAVQKGARLIALPENFSFIGQERENITFAEERETGE 65
Query: 269 TSRALSKXXXXXXXXXXXXXXPER--YEKKLYNTCTVWDDT 385
L K P R + K+ NTC V+D +
Sbjct: 66 IVHFLKKFSMKHSVAIIGGSVPLRSSSKAKVTNTCLVFDQS 106
>UniRef50_Q82UY9 Cluster: Carbon-nitrogen hydrolase; n=50;
Proteobacteria|Rep: Carbon-nitrogen hydrolase -
Nitrosomonas europaea
Length = 287
Score = 44.4 bits (100), Expect = 8e-04
Identities = 32/103 (31%), Positives = 48/103 (46%), Gaps = 4/103 (3%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYA--EEVP 259
++A +Q++ GP + N+ +A + I A K A+LV LPE F G K D+ A E
Sbjct: 22 RVAAVQMASGPSVAANLEEAFRLIEEAAAKQAKLVVLPEYF-CIMGMKDTDKLAVRENPG 80
Query: 260 SGETSRALSKXXXXXXXXXXXXXXP--ERYEKKLYNTCTVWDD 382
GE LS+ P K+YN+C V+D+
Sbjct: 81 EGEIQNFLSETAKRFGIWLAGGSVPLISPVSDKVYNSCLVYDE 123
>UniRef50_Q5L031 Cluster: Beta-alanine synthase; n=19; Bacteria|Rep:
Beta-alanine synthase - Geobacillus kaustophilus
Length = 296
Score = 44.4 bits (100), Expect = 8e-04
Identities = 29/91 (31%), Positives = 42/91 (46%), Gaps = 5/91 (5%)
Frame = +2
Query: 122 KSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY-----GTKYFDEYAEEVPSGETSRALS 286
K K I + VK + AK++GAQ++ L E F PY TK++ E AEE+P+G T++
Sbjct: 26 KEKAIEKHVKLVKEAKDRGAQIICLQEIFYGPYFCAEQNTKWY-EAAEEIPNGPTTKMFQ 84
Query: 287 KXXXXXXXXXXXXXXPERYEKKLYNTCTVWD 379
+ YNT V D
Sbjct: 85 EIAKQLGVVIVLPIYEREGIATYYNTAAVID 115
>UniRef50_Q9UYV8 Cluster: Beta ureidopropionase; n=4;
Thermococcaceae|Rep: Beta ureidopropionase - Pyrococcus
abyssi
Length = 262
Score = 44.0 bits (99), Expect = 0.001
Identities = 28/72 (38%), Positives = 45/72 (62%), Gaps = 6/72 (8%)
Frame = +2
Query: 86 KIALIQLS---VGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFD---EYA 247
K+A +Q++ + PDK N ++A K I A ++GAQLV LPE F++ Y + + E A
Sbjct: 3 KVAYVQMNPQILEPDK--NYSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEVFEIA 60
Query: 248 EEVPSGETSRAL 283
+++P GET+ L
Sbjct: 61 QKIPEGETTTFL 72
>UniRef50_Q97RA3 Cluster: Carbon-nitrogen hydrolase family protein;
n=24; Bacteria|Rep: Carbon-nitrogen hydrolase family
protein - Streptococcus pneumoniae
Length = 291
Score = 43.2 bits (97), Expect = 0.002
Identities = 19/48 (39%), Positives = 28/48 (58%)
Frame = +2
Query: 77 RGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY 220
R ++A IQ+ D + NI A + + A E+GAQ++ LPE F PY
Sbjct: 2 RNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPELFEHPY 49
>UniRef50_Q46AW4 Cluster: Putative amidohydrolase; n=1;
Methanosarcina barkeri str. Fusaro|Rep: Putative
amidohydrolase - Methanosarcina barkeri (strain Fusaro /
DSM 804)
Length = 287
Score = 42.7 bits (96), Expect = 0.002
Identities = 23/60 (38%), Positives = 37/60 (61%), Gaps = 1/60 (1%)
Frame = +2
Query: 86 KIALIQLSV-GPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
K+A IQ+ V K +N+ +A+ A KGA+L+ LPE F++ + ++FD AE +PS
Sbjct: 2 KVACIQMDVLHCRKQENLEKALHMALKAVRKGAELIVLPEVFSTGFCYEHFDHAAETLPS 61
>UniRef50_Q8FM85 Cluster: Putative uncharacterized protein; n=2;
Corynebacterium|Rep: Putative uncharacterized protein -
Corynebacterium efficiens
Length = 296
Score = 41.9 bits (94), Expect = 0.004
Identities = 22/60 (36%), Positives = 34/60 (56%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPSG 265
+IALIQ++ G DK N+ A +GA+L+ PE + +GT DE AE++ +G
Sbjct: 35 RIALIQITSGGDKMANLELVRTTATDAAAQGARLLIYPEATSQAFGTGRLDEQAEDLHTG 94
>UniRef50_Q606Z9 Cluster: Hydrolase, carbon-nitrogen family; n=38;
Bacteria|Rep: Hydrolase, carbon-nitrogen family -
Methylococcus capsulatus
Length = 295
Score = 41.9 bits (94), Expect = 0.004
Identities = 31/107 (28%), Positives = 47/107 (43%), Gaps = 4/107 (3%)
Frame = +2
Query: 71 LKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFD---- 238
+K ++AL+Q + + +N+A +V+ I +K KGA LV LPE PY + D
Sbjct: 1 MKSTIELALVQQACNGSREQNLAASVEGIRRSKAKGADLVMLPELHLGPYFCQTEDCSCF 60
Query: 239 EYAEEVPSGETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWD 379
+ AE +P G T+ L R +NT V D
Sbjct: 61 DGAETIP-GPTTAELGSVARELGVVVVASLFERRAPGLYHNTAVVLD 106
>UniRef50_A0BR54 Cluster: Chromosome undetermined scaffold_122,
whole genome shotgun sequence; n=2;
Oligohymenophorea|Rep: Chromosome undetermined
scaffold_122, whole genome shotgun sequence - Paramecium
tetraurelia
Length = 281
Score = 41.5 bits (93), Expect = 0.006
Identities = 32/101 (31%), Positives = 47/101 (46%), Gaps = 2/101 (1%)
Frame = +2
Query: 68 MLKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYA 247
MLK+ FKIA IQ ++ K++ +A +I A +G+++ L ECFNS Y A
Sbjct: 1 MLKK-FKIACIQNAITATKTQTLALVKDQIKEAAIQGSKVCILGECFNSYYVKAQLQNNA 59
Query: 248 EEV-PSGETSRA-LSKXXXXXXXXXXXXXXPERYEKKLYNT 364
E+ +GE L PE+ K+YNT
Sbjct: 60 EDFGKTGERQTLDLISEISKQFGIMIIGSIPEKSGDKMYNT 100
>UniRef50_Q8Y8V0 Cluster: Lmo0792 protein; n=12; Listeria|Rep:
Lmo0792 protein - Listeria monocytogenes
Length = 296
Score = 41.1 bits (92), Expect = 0.008
Identities = 23/61 (37%), Positives = 34/61 (55%), Gaps = 1/61 (1%)
Frame = +2
Query: 86 KIALIQLSVGPD-KSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
KIALIQ P+ K N+ A++ I A EKGA LV PE +++ Y + D + + +
Sbjct: 5 KIALIQQKAVPNNKEANLKLAIQYIKEAHEKGADLVLFPEMWSNGYAPPFEDAFNHPLAT 64
Query: 263 G 265
G
Sbjct: 65 G 65
>UniRef50_A0TTW8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=5;
Proteobacteria|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Burkholderia
cenocepacia MC0-3
Length = 299
Score = 40.7 bits (91), Expect = 0.010
Identities = 28/72 (38%), Positives = 38/72 (52%), Gaps = 4/72 (5%)
Frame = +2
Query: 77 RGFKIALIQLSVGPDKSK-NIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDE---Y 244
R F IA +Q+ ++ N+A A+ I A GA L+ LPE +S Y + DE
Sbjct: 15 RAFTIACVQMEPRIGAAQHNLATALDRIETAARNGAALIVLPELASSGYVFEDRDEALAL 74
Query: 245 AEEVPSGETSRA 280
AE VP G T+RA
Sbjct: 75 AELVPDGPTARA 86
>UniRef50_Q6SHH5 Cluster: Carbon-nitrogen hydrolase family protein;
n=2; environmental samples|Rep: Carbon-nitrogen
hydrolase family protein - uncultured bacterium 439
Length = 255
Score = 40.3 bits (90), Expect = 0.013
Identities = 29/99 (29%), Positives = 47/99 (47%), Gaps = 1/99 (1%)
Frame = +2
Query: 86 KIALIQLSVG-PDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
KIA I L++ D KN+ + K + AK G +V LPE FN+ + +YA E+P+
Sbjct: 2 KIATISLNIAWQDIEKNLERTEKFVRQAKADGCDVVVLPEVFNTGF-IADVGKYA-ELPN 59
Query: 263 GETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWD 379
+T AL + ++ +K +N V+D
Sbjct: 60 CKTHHALQQFALNNLINIVAGASEKQPNEKAHNIALVFD 98
>UniRef50_Q6N4F1 Cluster: Possible amidohydrolase; n=2;
Rhodopseudomonas palustris|Rep: Possible amidohydrolase
- Rhodopseudomonas palustris
Length = 557
Score = 39.9 bits (89), Expect = 0.017
Identities = 26/77 (33%), Positives = 41/77 (53%), Gaps = 4/77 (5%)
Frame = +2
Query: 68 MLKRGFKIALIQ-LSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY---GTKYF 235
M KR F+ A +Q L+ D NIA A + + A +GA+L+ PEC ++ Y ++
Sbjct: 1 MTKRRFRAAAVQTLAKLGDFDFNIALATRYVEDAARQGAELIVFPECMDTGYLFDSPEHC 60
Query: 236 DEYAEEVPSGETSRALS 286
E AE + G +AL+
Sbjct: 61 RELAETLTDGPFVKALA 77
>UniRef50_Q5C342 Cluster: SJCHGC04680 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC04680 protein - Schistosoma
japonicum (Blood fluke)
Length = 238
Score = 39.9 bits (89), Expect = 0.017
Identities = 22/57 (38%), Positives = 30/57 (52%)
Frame = +2
Query: 41 YITVLRRATMLKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFN 211
+I LR KI +IQ+ +K N QAVK I+ A G ++V LPECF+
Sbjct: 1 FIGKLREKLQYNMTTKIGVIQMQSTANKEWNFNQAVKYINKAIASGVKIVFLPECFD 57
>UniRef50_Q92DM8 Cluster: Lin0785 protein; n=5; Bacteria|Rep:
Lin0785 protein - Listeria innocua
Length = 296
Score = 39.5 bits (88), Expect = 0.023
Identities = 21/61 (34%), Positives = 33/61 (54%), Gaps = 1/61 (1%)
Frame = +2
Query: 86 KIALIQLSVGP-DKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
K+AL+Q P DK N+ ++K I A KGA LV PE +++ Y + + E + +
Sbjct: 5 KVALVQQQAVPNDKEANLNLSIKYIKEAHRKGADLVLFPEMWSNGYAPPFETAFDEPMDA 64
Query: 263 G 265
G
Sbjct: 65 G 65
>UniRef50_Q69NN4 Cluster: Putative uncharacterized protein
OSJNBb0015B15.28; n=1; Oryza sativa (japonica
cultivar-group)|Rep: Putative uncharacterized protein
OSJNBb0015B15.28 - Oryza sativa subsp. japonica (Rice)
Length = 87
Score = 39.5 bits (88), Expect = 0.023
Identities = 19/39 (48%), Positives = 25/39 (64%)
Frame = +2
Query: 83 FKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALP 199
+K+AL+QL V P K +NIA+A + A GA LV LP
Sbjct: 16 YKVALLQLPVSPRKEENIARARARLDAAAAAGATLVVLP 54
>UniRef50_A5V962 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Sphingomonas
wittichii RW1|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Sphingomonas
wittichii RW1
Length = 268
Score = 39.1 bits (87), Expect = 0.031
Identities = 27/102 (26%), Positives = 41/102 (40%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPSG 265
KI ++Q++VG DK NIA+ +++ G +V LPE + G + A E G
Sbjct: 2 KIGVVQINVGMDKEANIARLDRQVRRLAADGCDIVFLPEMAMALTGKPAALQAAAEAEDG 61
Query: 266 ETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWDDTXE 391
A+ ER + NT V+D E
Sbjct: 62 AYVTAMKALAKECGINLHLGSFMERRGDRFLNTSLVFDRQGE 103
>UniRef50_A5GU42 Cluster: Nitrilase-related protein; n=1;
Synechococcus sp. RCC307|Rep: Nitrilase-related protein
- Synechococcus sp. (strain RCC307)
Length = 305
Score = 39.1 bits (87), Expect = 0.031
Identities = 21/62 (33%), Positives = 32/62 (51%), Gaps = 5/62 (8%)
Frame = +2
Query: 89 IALIQLSVGPDKSKNIAQAVKEIHLAKEKGA-----QLVALPECFNSPYGTKYFDEYAEE 253
+AL+Q V P+ N Q + A + +L+ LPE +NSPY + F E+AE
Sbjct: 7 VALVQFQVSPEPQVNRQQVCHWLEQAMTQAGTSSSPKLLMLPEVWNSPYQAERFAEFAEP 66
Query: 254 VP 259
+P
Sbjct: 67 IP 68
>UniRef50_A0JSW0 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Arthrobacter sp.
FB24|Rep: Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Arthrobacter sp. (strain FB24)
Length = 294
Score = 38.7 bits (86), Expect = 0.040
Identities = 21/65 (32%), Positives = 32/65 (49%)
Frame = +2
Query: 44 ITVLRRATMLKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYG 223
+ +L+ L+ ++AL Q+ G D S+N+ K AK+ GAQLV PE +G
Sbjct: 18 VAILKGHAKLEVIVRVALAQIVTGRDISRNLDIVEKYARKAKKGGAQLVVFPEATMRAFG 77
Query: 224 TKYFD 238
D
Sbjct: 78 NSLLD 82
>UniRef50_O76463 Cluster: Nitrilase and fragile histidine triad
fusion protein NitFhit [Includes:
Bis(5'-adenosyl)-triphosphatase (EC 3.6.1.29)
(Diadenosine 5',5'''-P1,P3-triphosphate hydrolase)
(Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase);
Nitrilase homolog (EC 3.5.-.-)]; n=4; Bilateria|Rep:
Nitrilase and fragile histidine triad fusion protein
NitFhit [Includes: Bis(5'-adenosyl)-triphosphatase (EC
3.6.1.29) (Diadenosine 5',5'''-P1,P3-triphosphate
hydrolase) (Dinucleosidetriphosphatase) (AP3A hydrolase)
(AP3Aase); Nitrilase homolog (EC 3.5.-.-)] -
Caenorhabditis elegans
Length = 440
Score = 38.7 bits (86), Expect = 0.040
Identities = 23/55 (41%), Positives = 29/55 (52%)
Frame = +2
Query: 47 TVLRRATMLKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFN 211
TV RR R F IA+ Q++ D KN A I A EK ++V LPECF+
Sbjct: 4 TVFRRTMATGRHF-IAVCQMTSDNDLEKNFQAAKNMIERAGEKKCEMVFLPECFD 57
>UniRef50_Q0F1V1 Cluster: Hydrolase, carbon-nitrogen family protein;
n=1; Mariprofundus ferrooxydans PV-1|Rep: Hydrolase,
carbon-nitrogen family protein - Mariprofundus
ferrooxydans PV-1
Length = 272
Score = 38.3 bits (85), Expect = 0.053
Identities = 18/48 (37%), Positives = 27/48 (56%)
Frame = +2
Query: 68 MLKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFN 211
M G ++A IQ++ G D+ N+ QA + A GA+L LPE F+
Sbjct: 1 MSSAGMRVACIQMNSGADREANLEQASLLLQQAASAGAELAVLPENFS 48
>UniRef50_A4J4S3 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Desulfotomaculum
reducens MI-1|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Desulfotomaculum
reducens MI-1
Length = 273
Score = 38.3 bits (85), Expect = 0.053
Identities = 27/78 (34%), Positives = 40/78 (51%), Gaps = 5/78 (6%)
Frame = +2
Query: 71 LKRGFKIALIQLS-VGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFD--- 238
+KR I LIQ+ V D + N+A+A++ I A GAQ++ LPE + Y +
Sbjct: 1 MKRPLTIGLIQMDCVLGDVAANVAKAIERIRQAAAMGAQIICLPELCTTGYRPDLLEDKL 60
Query: 239 -EYAEEVPSGETSRALSK 289
E E VP G T+ S+
Sbjct: 61 WELTEPVP-GPTTDVFSQ 77
>UniRef50_Q9ZMC7 Cluster: Putative; n=6; Campylobacterales|Rep:
Putative - Helicobacter pylori J99 (Campylobacter pylori
J99)
Length = 294
Score = 37.9 bits (84), Expect = 0.071
Identities = 35/111 (31%), Positives = 48/111 (43%), Gaps = 11/111 (9%)
Frame = +2
Query: 74 KRGFKIALIQLSVGPDK-SKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFD---- 238
KR K A+IQ+ P ++N+ A+ A KGA L+ LPE F+S Y D
Sbjct: 8 KRILKTAVIQMQSKPYALNENLQLALNLAKEAHNKGANLIVLPELFDSGYCVNDKDADFG 67
Query: 239 ------EYAEEVPSGETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTV 373
E+ EE ET RALS E+ KKLY++ +
Sbjct: 68 LDFKAIEHGEETLKNETLRALSDFAKSSDTHIVACSI-EKNNKKLYDSAYI 117
>UniRef50_Q1AWK1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=4; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Rubrobacter xylanophilus (strain DSM
9941 / NBRC 16129)
Length = 276
Score = 37.1 bits (82), Expect = 0.12
Identities = 31/97 (31%), Positives = 44/97 (45%), Gaps = 2/97 (2%)
Frame = +2
Query: 92 ALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPSGET 271
A IQ+S PD+ +N A I A GA LVALPE ++ + + E AE +P G T
Sbjct: 9 AAIQMSSTPDRGENRRVAEALIREAAAAGATLVALPELWSCHGLEEVYRENAEPIP-GPT 67
Query: 272 SRALSKXXXXXXXXXXXXXXPERY--EKKLYNTCTVW 376
+ L ER ++L NT T++
Sbjct: 68 TEFLGSLARELGIYLLGGSILERVSGSERLGNTSTLY 104
>UniRef50_Q4JAH2 Cluster: Conserved protein; n=4; Sulfolobaceae|Rep:
Conserved protein - Sulfolobus acidocaldarius
Length = 297
Score = 36.3 bits (80), Expect = 0.22
Identities = 27/68 (39%), Positives = 36/68 (52%), Gaps = 1/68 (1%)
Frame = +2
Query: 89 IALIQLSVGPDKSK-NIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPSG 265
I L+ L + K NI +A + I +AKEKGA+LV LP F P G F+ Y E S
Sbjct: 3 IGLLHLRLKEASRKANIEKARRLIKVAKEKGAKLVVLPSLF--PIG-NLFEVYENEKKSR 59
Query: 266 ETSRALSK 289
R L++
Sbjct: 60 SVIRNLAE 67
>UniRef50_Q74H63 Cluster: Hydrolase, carbon-nitrogen family; n=8;
Desulfuromonadales|Rep: Hydrolase, carbon-nitrogen
family - Geobacter sulfurreducens
Length = 259
Score = 35.9 bits (79), Expect = 0.28
Identities = 27/104 (25%), Positives = 44/104 (42%), Gaps = 1/104 (0%)
Frame = +2
Query: 71 LKRGFKIALIQLSVG-PDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYA 247
+ R K +Q +V D N+A K + +G +L LPE +++ Y K +E A
Sbjct: 1 MNRTVKAGAVQFTVRLGDIDANVAYVQKALRRLASQGCRLAVLPEMWSTGYAYKELNELA 60
Query: 248 EEVPSGETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWD 379
+ P E L + PE + +K++NT V D
Sbjct: 61 KRTP--EVVAELGR-LSRELEMVIVGSMPEPHGEKVFNTAYVLD 101
>UniRef50_Q9ABL5 Cluster: Hydrolase, carbon-nitrogen family; n=13;
Bacteria|Rep: Hydrolase, carbon-nitrogen family -
Caulobacter crescentus (Caulobacter vibrioides)
Length = 292
Score = 35.5 bits (78), Expect = 0.38
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +2
Query: 71 LKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY 220
+ R +A IQ S G D NI + I A KGAQ++ E F PY
Sbjct: 1 MARTLSVAAIQTSYGMDLQANIKKTEGFIREAASKGAQVILPSELFQGPY 50
>UniRef50_Q6JHR5 Cluster: Aliphatic amidase; n=1; Saccharopolyspora
spinosa|Rep: Aliphatic amidase - Saccharopolyspora
spinosa
Length = 308
Score = 35.5 bits (78), Expect = 0.38
Identities = 19/46 (41%), Positives = 27/46 (58%), Gaps = 1/46 (2%)
Frame = +2
Query: 86 KIALIQL-SVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY 220
++ L+Q SV D + NI AV E+ A E+GA L+ PEC+ Y
Sbjct: 21 RVGLVQSGSVLGDVAANIDTAVNEVISAAERGADLLVFPECYLHGY 66
>UniRef50_Q12ZA5 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Methanococcoides
burtonii DSM 6242|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Methanococcoides
burtonii (strain DSM 6242)
Length = 270
Score = 35.5 bits (78), Expect = 0.38
Identities = 23/66 (34%), Positives = 37/66 (56%), Gaps = 6/66 (9%)
Frame = +2
Query: 71 LKRGFK-----IALIQLSVGP-DKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKY 232
+KRG+K IA IQ+ + +K KNI +A+ A KGA ++ LPE F++ + +
Sbjct: 1 MKRGYKVETIKIAAIQMDICHCNKQKNIKKALHFSEEAISKGADIIVLPEVFSTGFCYEE 60
Query: 233 FDEYAE 250
+ AE
Sbjct: 61 LENIAE 66
>UniRef50_Q60BT4 Cluster: Hydrolase, carbon-nitrogen family; n=15;
Proteobacteria|Rep: Hydrolase, carbon-nitrogen family -
Methylococcus capsulatus
Length = 273
Score = 35.1 bits (77), Expect = 0.50
Identities = 16/39 (41%), Positives = 24/39 (61%)
Frame = +2
Query: 92 ALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECF 208
A +Q++ GP N+ +A + + A E GA+LV LPE F
Sbjct: 7 AAVQMASGPQVGSNLLEAGRLVKQAAEAGARLVVLPENF 45
>UniRef50_Q1JW05 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Desulfuromonas
acetoxidans DSM 684|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Desulfuromonas
acetoxidans DSM 684
Length = 153
Score = 35.1 bits (77), Expect = 0.50
Identities = 28/105 (26%), Positives = 47/105 (44%), Gaps = 2/105 (1%)
Frame = +2
Query: 71 LKRGFKIALIQLSVGPDK-SKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYA 247
+K A +Q ++ + N+A A + + +GAQL LPE +++ Y ++ + A
Sbjct: 1 MKHDVTAAAVQFNISLGEIDHNLATASEGLRRVAAQGAQLAVLPEMWSTGYDYRHLSDLA 60
Query: 248 EEVPSG-ETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWD 379
E+ P T ++LS PE+ LYNT V D
Sbjct: 61 EQTPRVLITLQSLS----LELKLVIVGSLPEKDGNALYNTLYVID 101
>UniRef50_Q8TLM7 Cluster: Carbon-nitrogen hydrolase; n=2;
Methanosarcina|Rep: Carbon-nitrogen hydrolase -
Methanosarcina acetivorans
Length = 309
Score = 35.1 bits (77), Expect = 0.50
Identities = 20/60 (33%), Positives = 34/60 (56%), Gaps = 1/60 (1%)
Frame = +2
Query: 86 KIALIQLSVGP-DKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
K+A IQ+ V K +N+ +A+ A +KGA+L+ PE F + + + + AE +PS
Sbjct: 2 KVACIQMDVLQCKKQENLEKALYMALKAVKKGAELIVFPEVFTTGFCYEDLNHAAESLPS 61
>UniRef50_Q6L0F7 Cluster: Carbon-nitrogen hydrolase family; n=2;
Thermoplasmatales|Rep: Carbon-nitrogen hydrolase family
- Picrophilus torridus
Length = 256
Score = 35.1 bits (77), Expect = 0.50
Identities = 20/58 (34%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFN-SPYGTKYFDEYAEEV 256
+IAL Q+ DK N+ + K +A GA L+ PE F KY +E AE +
Sbjct: 3 RIALTQIHSSMDKESNLEKLRKYTEIAASNGADLIVFPEYFMFYSNDKKYLNENAEPI 60
>UniRef50_A6DN63 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Lentisphaera
araneosa HTCC2155|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Lentisphaera araneosa
HTCC2155
Length = 292
Score = 34.7 bits (76), Expect = 0.66
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPEC 205
++ L+Q+S PD +N+A A I A + +L+ PEC
Sbjct: 27 RVCLVQMSSSPDFEENLAHAKSIIEQASQNRDELIIFPEC 66
>UniRef50_A4B9A7 Cluster: Probable hydratase; n=2; Bacteria|Rep:
Probable hydratase - Reinekea sp. MED297
Length = 289
Score = 34.3 bits (75), Expect = 0.87
Identities = 17/48 (35%), Positives = 25/48 (52%)
Frame = +2
Query: 77 RGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY 220
R +A Q+ G D S+N+ A + + A GAQ++ L E F PY
Sbjct: 2 REVTVAATQMPCGWDVSENLKTAERLVREAAASGAQVILLQELFERPY 49
>UniRef50_A0RYH6 Cluster: Amidohydrolase; n=1; Cenarchaeum
symbiosum|Rep: Amidohydrolase - Cenarchaeum symbiosum
Length = 269
Score = 34.3 bits (75), Expect = 0.87
Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPE--CFNSPYG 223
++A+ QL DK +N+ + VK + A GA LVA PE F +P G
Sbjct: 3 RVAVAQLRASTDKDRNLRRIVKYVSEAAAGGAGLVAFPEFMMFYTPPG 50
>UniRef50_P55175 Cluster: UPF0012 hydrolase sll0601; n=40;
Cyanobacteria|Rep: UPF0012 hydrolase sll0601 -
Synechocystis sp. (strain PCC 6803)
Length = 272
Score = 34.3 bits (75), Expect = 0.87
Identities = 16/39 (41%), Positives = 27/39 (69%)
Frame = +2
Query: 92 ALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECF 208
A +Q++ P+ ++N+ +A + I LA +GA+LV LPE F
Sbjct: 7 AALQMTSRPNLTENLQEAEELIDLAVRQGAELVGLPENF 45
>UniRef50_Q0AM99 Cluster: Peptidase S8 and S53, subtilisin, kexin,
sedolisin precursor; n=1; Maricaulis maris MCS10|Rep:
Peptidase S8 and S53, subtilisin, kexin, sedolisin
precursor - Maricaulis maris (strain MCS10)
Length = 959
Score = 33.9 bits (74), Expect = 1.1
Identities = 19/54 (35%), Positives = 27/54 (50%)
Frame = +2
Query: 26 HSNATYITVLRRATMLKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQL 187
HSN Y+TV R F IA+ L VG S+++A A E ++ + A L
Sbjct: 558 HSNKHYVTVFEDVGNSFRDFTIAVDDLIVGSSLSESVANAYAEQYIYERTSASL 611
>UniRef50_A0BLB1 Cluster: Chromosome undetermined scaffold_114,
whole genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_114,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 284
Score = 33.9 bits (74), Expect = 1.1
Identities = 25/102 (24%), Positives = 43/102 (42%), Gaps = 2/102 (1%)
Frame = +2
Query: 83 FKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEV-- 256
+K+ALIQ +V K K + I +K +++ L E FN+ + T + AE+
Sbjct: 7 YKVALIQNAVFETKQKILEGVAASIRDCVQKECKVIFLGEFFNTIFETNQLKKNAEDFSD 66
Query: 257 PSGETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWDD 382
+ + L K PE + KL+N ++D
Sbjct: 67 KNNRETYELMKQLSEEFQIMIIGGLPEVADGKLFNAALAFND 108
>UniRef50_Q972L1 Cluster: 281aa long hypothetical
beta-ureidopropionase; n=1; Sulfolobus tokodaii|Rep:
281aa long hypothetical beta-ureidopropionase -
Sulfolobus tokodaii
Length = 281
Score = 33.9 bits (74), Expect = 1.1
Identities = 24/78 (30%), Positives = 37/78 (47%), Gaps = 5/78 (6%)
Frame = +2
Query: 71 LKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY-----GTKYF 235
+K KIA+IQ+ K NI +A++ A + GA+L+ E F + Y K+F
Sbjct: 1 MKHMVKIAMIQMGSVESKEANIQKALEYTKAAVKDGAELIVYNELFTTQYFPATEDPKFF 60
Query: 236 DEYAEEVPSGETSRALSK 289
D E G T R ++
Sbjct: 61 D--LAEPEDGPTVRVFAE 76
>UniRef50_O76464 Cluster: Nitrilase and fragile histidine triad
fusion protein NitFhit (NFT-1 protein) [Includes:
Bis(5'-adenosyl)-triphosphatase (EC 3.6.1.29)
(Diadenosine 5',5'''-P1,P3-triphosphate hydrolase)
(Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase);
Nitrilase homolog (EC 3.5.-.-)]; n=18; Eumetazoa|Rep:
Nitrilase and fragile histidine triad fusion protein
NitFhit (NFT-1 protein) [Includes:
Bis(5'-adenosyl)-triphosphatase (EC 3.6.1.29)
(Diadenosine 5',5'''-P1,P3-triphosphate hydrolase)
(Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3Aase);
Nitrilase homolog (EC 3.5.-.-)] - Drosophila
melanogaster (Fruit fly)
Length = 460
Score = 33.9 bits (74), Expect = 1.1
Identities = 19/54 (35%), Positives = 32/54 (59%), Gaps = 3/54 (5%)
Frame = +2
Query: 53 LRRATMLKR---GFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPEC 205
LRR ++ KR IA+ Q+ DK+ N++Q ++ + AK + A ++ LPEC
Sbjct: 20 LRRMSVQKRKDQSATIAVGQMRSTSDKAANLSQVIELVDRAKSQNACMLFLPEC 73
>UniRef50_Q2S196 Cluster: Hydrolase, carbon-nitrogen family; n=1;
Salinibacter ruber DSM 13855|Rep: Hydrolase,
carbon-nitrogen family - Salinibacter ruber (strain DSM
13855)
Length = 283
Score = 33.5 bits (73), Expect = 1.5
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = +2
Query: 89 IALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY 220
IAL+Q +V P + + V+ + A + GA LV PE +P+
Sbjct: 3 IALVQHAVSPASPPRVDRGVRAVQAAADAGADLVVFPELSFTPF 46
>UniRef50_Q1GTC5 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=9; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Sphingopyxis alaskensis
(Sphingomonas alaskensis)
Length = 300
Score = 33.5 bits (73), Expect = 1.5
Identities = 19/68 (27%), Positives = 32/68 (47%), Gaps = 2/68 (2%)
Frame = +2
Query: 71 LKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDE--Y 244
+ R +A +QL++ NI + A +GAQ++ PE F PY + +E +
Sbjct: 18 MTRTITVAALQLALPGPVEPNIKAVTALVEAAAARGAQIILPPELFEGPYFCQVEEEELF 77
Query: 245 AEEVPSGE 268
A P+ E
Sbjct: 78 ATARPTAE 85
>UniRef50_O30121 Cluster: Putative uncharacterized protein; n=1;
Archaeoglobus fulgidus|Rep: Putative uncharacterized
protein - Archaeoglobus fulgidus
Length = 257
Score = 33.5 bits (73), Expect = 1.5
Identities = 17/45 (37%), Positives = 26/45 (57%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY 220
+IAL Q + PD+ NI + + I A + A +V LPE FN+ +
Sbjct: 8 RIALAQQRILPDREVNIMKGMSLIKRAIQVRADMVILPEVFNTGF 52
>UniRef50_Q6RWN7 Cluster: Nitrilase; n=21; root|Rep: Nitrilase -
uncultured organism
Length = 353
Score = 33.1 bits (72), Expect = 2.0
Identities = 20/52 (38%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = +2
Query: 68 MLKRGFKIALIQLS-VGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY 220
M + K+A IQ++ V D+ IA+A + I A GA+LV PE F Y
Sbjct: 1 MAESKLKVAAIQVAPVFMDRDATIARACERIAEAARAGAELVVFPEAFVPGY 52
>UniRef50_A4U2A6 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=3;
Magnetospirillum|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Magnetospirillum
gryphiswaldense
Length = 279
Score = 33.1 bits (72), Expect = 2.0
Identities = 16/45 (35%), Positives = 26/45 (57%)
Frame = +2
Query: 68 MLKRGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPE 202
M+ FK A +Q++ G D + NI A + A+ GA+L+ +PE
Sbjct: 2 MIGDTFKAACLQVNAGTDMTDNIDAAARLAVEARAAGAELILMPE 46
>UniRef50_Q183H2 Cluster: Putative carbon-nitrogen hydrolase; n=2;
Clostridium difficile|Rep: Putative carbon-nitrogen
hydrolase - Clostridium difficile (strain 630)
Length = 268
Score = 32.3 bits (70), Expect = 3.5
Identities = 20/49 (40%), Positives = 29/49 (59%), Gaps = 1/49 (2%)
Frame = +2
Query: 77 RGFKIALIQL-SVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY 220
R KI +IQ SV + KNI +AV+ I ++GA ++ LPE F + Y
Sbjct: 3 RKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLPELFATGY 51
>UniRef50_Q4Q7M9 Cluster: Putative uncharacterized protein; n=6;
Trypanosomatidae|Rep: Putative uncharacterized protein -
Leishmania major
Length = 1057
Score = 32.3 bits (70), Expect = 3.5
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = -1
Query: 269 SPPMALPLHIHQNTWFRKES*STPARPPIE 180
SPP+ P+ I Q W R E+ + P+ PP E
Sbjct: 814 SPPIVSPMPIQQTPWLRYENPAPPSPPPQE 843
>UniRef50_Q88EJ9 Cluster: Carbon-nitrogen hydrolase family protein;
n=1; Pseudomonas putida KT2440|Rep: Carbon-nitrogen
hydrolase family protein - Pseudomonas putida (strain
KT2440)
Length = 273
Score = 31.9 bits (69), Expect = 4.6
Identities = 23/69 (33%), Positives = 38/69 (55%), Gaps = 1/69 (1%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEK-GAQLVALPECFNSPYGTKYFDEYAEEVPS 262
K++LIQ++ DK+ N+A+A + A ++ G++LV PE F+ GT A E S
Sbjct: 2 KVSLIQVNSVQDKAFNLAEADRLAREAIDRDGSRLVVFPEHFDWAGGTPEQKIAAGEPHS 61
Query: 263 GETSRALSK 289
G + + K
Sbjct: 62 GGPAYEMCK 70
>UniRef50_Q7UWX1 Cluster: Beta-alanine synthetase; n=1; Pirellula
sp.|Rep: Beta-alanine synthetase - Rhodopirellula
baltica
Length = 303
Score = 31.9 bits (69), Expect = 4.6
Identities = 26/107 (24%), Positives = 48/107 (44%), Gaps = 1/107 (0%)
Frame = +2
Query: 74 KRGFKIALIQL-SVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAE 250
K+ ++A+ Q+ + D+ N+ + + A KGA++V LPE + E A
Sbjct: 52 KKVCRVAMAQIYCIDGDREGNLRRIENAVEEASAKGAEIVCLPETCLYGWVNAKAHELAH 111
Query: 251 EVPSGETSRALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWDDTXE 391
+P G+ + ALS+ E+ +LY++ + DD E
Sbjct: 112 PIP-GKDTDALSE-IAKKNRVFLSVGLSEKEGDQLYDSVVLIDDEGE 156
>UniRef50_Q025F3 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Solibacter
usitatus Ellin6076|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Solibacter usitatus
(strain Ellin6076)
Length = 263
Score = 31.9 bits (69), Expect = 4.6
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = +2
Query: 83 FKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY 220
F+IAL + +++A A + I A +GA ++ PECF Y
Sbjct: 4 FRIALANIRFPATPDESVALAEQAIAQASREGAGIICFPECFVPGY 49
>UniRef50_Q971J8 Cluster: Putative uncharacterized protein STS165;
n=1; Sulfolobus tokodaii|Rep: Putative uncharacterized
protein STS165 - Sulfolobus tokodaii
Length = 73
Score = 31.9 bits (69), Expect = 4.6
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +2
Query: 146 VKEIHLAKEKGAQLVALPECFNSPYGTKYFDEY 244
V E+HL K+ Q+ +P C + P T YF Y
Sbjct: 14 VTEVHLTKKSYNQINVMPRCSDFPITTTYFTTY 46
>UniRef50_A4SNH5 Cluster: Amidohydrolase family protein; n=2;
Proteobacteria|Rep: Amidohydrolase family protein -
Aeromonas salmonicida (strain A449)
Length = 284
Score = 31.5 bits (68), Expect = 6.1
Identities = 14/41 (34%), Positives = 23/41 (56%)
Frame = +2
Query: 86 KIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECF 208
++A++Q+ G D N+ QA + A +GA+ LPE F
Sbjct: 12 RVAVLQMVSGDDLDHNLTQAEALLRQAAAEGAEFALLPEYF 52
>UniRef50_A4ALG5 Cluster: Putative hydrolase; n=2; Actinobacteria
(class)|Rep: Putative hydrolase - marine actinobacterium
PHSC20C1
Length = 271
Score = 31.5 bits (68), Expect = 6.1
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2
Query: 89 IALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPE 202
+A+ Q + G D+ +NIA + A E+GA V PE
Sbjct: 7 VAVAQFAPGADRDENIATVTQLAERAVERGANFVVFPE 44
>UniRef50_A2ICY3 Cluster: Cyanide hydratase; n=23;
Gammaproteobacteria|Rep: Cyanide hydratase - Pseudomonas
aeruginosa
Length = 282
Score = 31.5 bits (68), Expect = 6.1
Identities = 17/40 (42%), Positives = 24/40 (60%)
Frame = +2
Query: 89 IALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECF 208
IA+IQ+ D + N+A A + + A E GA+L LPE F
Sbjct: 3 IAVIQMVSQDDVTANLAAARRLLEQAAEGGARLAVLPENF 42
>UniRef50_A0J1U1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Shewanella woodyi
ATCC 51908|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Shewanella woodyi
ATCC 51908
Length = 288
Score = 31.5 bits (68), Expect = 6.1
Identities = 20/48 (41%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = +2
Query: 68 MLKRGFKIALIQLSVGPDK-SKNIAQAVKEIHLAKEKGAQLVALPECF 208
M+ K A+IQL + S N+ +A + I A + GAQLV LPE F
Sbjct: 1 MITEPIKTAVIQLECKLSRESGNMRRAERYIKKAIKDGAQLVCLPESF 48
>UniRef50_A7QG85 Cluster: Chromosome undetermined scaffold_91, whole
genome shotgun sequence; n=4; core eudicotyledons|Rep:
Chromosome undetermined scaffold_91, whole genome
shotgun sequence - Vitis vinifera (Grape)
Length = 165
Score = 31.5 bits (68), Expect = 6.1
Identities = 20/54 (37%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +2
Query: 104 LSVGPDKS-KNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
LSVGP K + + K+ K++ A LV + +N Y T YF AEE P+
Sbjct: 107 LSVGPAKEPEKKKEEPKKKEDPKDQWADLVKAYQAYNPHYTTHYFVRSAEEDPN 160
>UniRef50_Q0W654 Cluster: Putative amidohydrolase; n=1; uncultured
methanogenic archaeon RC-I|Rep: Putative amidohydrolase
- Uncultured methanogenic archaeon RC-I
Length = 330
Score = 31.5 bits (68), Expect = 6.1
Identities = 22/70 (31%), Positives = 35/70 (50%), Gaps = 2/70 (2%)
Frame = +2
Query: 86 KIALIQL--SVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVP 259
++A +Q+ +G ++ N+ +A I A +GAQLV LPE S Y + A E
Sbjct: 13 RVAAVQMRSEIG-ERESNLKRATPLIEKAAREGAQLVVLPEMAASGYSIENSMWIAAEPV 71
Query: 260 SGETSRALSK 289
G T + L +
Sbjct: 72 DGPTVQWLKE 81
>UniRef50_Q1YU23 Cluster: Hydrolase, carbon-nitrogen family protein;
n=1; gamma proteobacterium HTCC2207|Rep: Hydrolase,
carbon-nitrogen family protein - gamma proteobacterium
HTCC2207
Length = 281
Score = 31.1 bits (67), Expect = 8.1
Identities = 18/44 (40%), Positives = 23/44 (52%)
Frame = +2
Query: 77 RGFKIALIQLSVGPDKSKNIAQAVKEIHLAKEKGAQLVALPECF 208
R F A +QL +N+A A I A E G++LV LPE F
Sbjct: 5 RSFIAAAVQLRPQQSLQQNLAAAGALIEQAAEAGSRLVVLPENF 48
>UniRef50_A6Q8M5 Cluster: Carbon-nitrogen hydrolase family protein;
n=1; Sulfurovum sp. NBC37-1|Rep: Carbon-nitrogen
hydrolase family protein - Sulfurovum sp. (strain
NBC37-1)
Length = 377
Score = 31.1 bits (67), Expect = 8.1
Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
Frame = +2
Query: 47 TVLRRATMLKRGFKIALIQLSVGPDK---SKNIAQAVKEIHLAKEKGAQLVALPECFNSP 217
+V +A L +G ++ + Q + +KN+ + I LAKEK QL++ PE +
Sbjct: 51 SVYEKAPKLGKGIRLGIYQAQAVSGEGATAKNLKRMEHAIRLAKEKHIQLLSFPELYIPG 110
Query: 218 Y 220
Y
Sbjct: 111 Y 111
>UniRef50_A4M5M1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Petrotoga mobilis
SJ95|Rep: Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Petrotoga mobilis SJ95
Length = 276
Score = 31.1 bits (67), Expect = 8.1
Identities = 25/67 (37%), Positives = 36/67 (53%), Gaps = 6/67 (8%)
Frame = +2
Query: 95 LIQL-SVGPDKSKNIAQAVKEIHLAKE-KGAQLVALPECFNSPYGTKYFDEYAEE----V 256
L+QL S DK N+ + + ++KE K A L LPE FN Y + + YAE +
Sbjct: 7 LVQLNSKLNDKGTNLKKL--DSLISKEVKKADLYILPEFFNIGYDLESINNYAENLAEII 64
Query: 257 PSGETSR 277
P GET++
Sbjct: 65 PDGETTQ 71
>UniRef50_A1IEG0 Cluster: Amidohydrolase-like precursor; n=1;
Candidatus Desulfococcus oleovorans Hxd3|Rep:
Amidohydrolase-like precursor - Candidatus Desulfococcus
oleovorans Hxd3
Length = 329
Score = 31.1 bits (67), Expect = 8.1
Identities = 22/69 (31%), Positives = 32/69 (46%), Gaps = 1/69 (1%)
Frame = +2
Query: 86 KIALIQLSVGPDK-SKNIAQAVKEIHLAKEKGAQLVALPECFNSPYGTKYFDEYAEEVPS 262
K+A +Q++ + KN+ A A ++GAQ+V LPE F S G + A
Sbjct: 17 KVAAVQMTALVGRVEKNMRAAEALAKEAFDQGAQMVILPEFFTSAMGFSQKMDRAARPVD 76
Query: 263 GETSRALSK 289
G R L K
Sbjct: 77 GAPMRLLQK 85
>UniRef50_A0GGV1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase precursor; n=1;
Burkholderia phytofirmans PsJN|Rep: Nitrilase/cyanide
hydratase and apolipoprotein N-acyltransferase precursor
- Burkholderia phytofirmans PsJN
Length = 292
Score = 31.1 bits (67), Expect = 8.1
Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 1/55 (1%)
Frame = +2
Query: 59 RATMLKRGFKIALIQLS-VGPDKSKNIAQAVKEIHLAKEKGAQLVALPECFNSPY 220
+A + + +IA Q + D + NIA+ V+ LA + GA+LV PE F + Y
Sbjct: 6 QALLPQTSLRIAAAQAQPISGDVTGNIARTVELTALAADAGAKLVVFPEKFLTGY 60
>UniRef50_A1D103 Cluster: Hydrolase, carbon-nitrogen family protein;
n=4; Pezizomycotina|Rep: Hydrolase, carbon-nitrogen
family protein - Neosartorya fischeri (strain ATCC 1020
/ DSM 3700 / NRRL 181)(Aspergillus fischerianus (strain
ATCC 1020 / DSM 3700 / NRRL 181))
Length = 311
Score = 31.1 bits (67), Expect = 8.1
Identities = 20/40 (50%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +2
Query: 86 KIALIQLSVGPDK-SKNIAQAVKEIHLAKEKGAQLVALPE 202
KIA+IQL V P K + N A+AVK I A + L LPE
Sbjct: 6 KIAVIQLYVKPLKPADNFARAVKFIREAAAQECHLAVLPE 45
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 370,385,082
Number of Sequences: 1657284
Number of extensions: 6653509
Number of successful extensions: 14722
Number of sequences better than 10.0: 84
Number of HSP's better than 10.0 without gapping: 14435
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 14708
length of database: 575,637,011
effective HSP length: 92
effective length of database: 423,166,883
effective search space used: 16503508437
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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