Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0373
(448 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g60930.1 68416.m06816 expressed protein 33 0.088
At4g10070.1 68417.m01647 KH domain-containing protein DNA-direct... 33 0.12
At1g79400.1 68414.m09253 cation/proton exchanger, putative (CHX2... 29 1.4
At3g48740.1 68416.m05322 nodulin MtN3 family protein similar to ... 29 1.9
At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64... 28 2.5
At3g21220.1 68416.m02682 mitogen-activated protein kinase kinase... 28 3.3
At5g08580.1 68418.m01021 calcium-binding EF hand family protein ... 27 4.4
At4g11720.1 68417.m01870 hypothetical protein 27 4.4
At3g60620.1 68416.m06783 phosphatidate cytidylyltransferase fami... 27 4.4
At2g45150.3 68415.m05621 phosphatidate cytidylyltransferase fami... 27 5.8
At2g45150.2 68415.m05619 phosphatidate cytidylyltransferase fami... 27 5.8
At2g45150.1 68415.m05620 phosphatidate cytidylyltransferase fami... 27 5.8
At2g25870.1 68415.m03105 haloacid dehalogenase-like hydrolase fa... 27 5.8
At1g24490.1 68414.m03084 60 kDa inner membrane family protein si... 27 5.8
At5g20580.2 68418.m02444 expressed protein predicted protein, Ar... 27 7.6
At1g09830.1 68414.m01105 phosphoribosylamine--glycine ligase (PU... 27 7.6
>At3g60930.1 68416.m06816 expressed protein
Length = 798
Score = 33.1 bits (72), Expect = 0.088
Identities = 25/84 (29%), Positives = 40/84 (47%), Gaps = 3/84 (3%)
Frame = +1
Query: 160 RVRDIGRLRARAERDPAGLGFVLPHAAAERGDLAHRRDALDPALRVPL---PAPHPRHEL 330
R+ + G + RA+ + G V+P A A + RD ++P P+ PA E
Sbjct: 413 RLVNTGLISPRADPEATQDGNVIPDATAPQTAPEASRDGVNPYTAGPVDAAPAEAQGAEP 472
Query: 331 AAVHPDAGLALHGLGRVHGLRVDV 402
+A P+A LAL + + G R+ V
Sbjct: 473 SAAAPEAVLALPAIDKAAGKRIRV 496
>At4g10070.1 68417.m01647 KH domain-containing protein DNA-directed
RNA polymerase (EC 2.7.7.6) II largestchain - mouse,
PIR2:A28490
Length = 725
Score = 32.7 bits (71), Expect = 0.12
Identities = 12/53 (22%), Positives = 28/53 (52%)
Frame = -1
Query: 160 EAHAAQTLQQSQHDNEDDVPIILDAEHRYHEDEHQRRLPAHHHELGDDVREQN 2
E + LQ+ + +N+D VP++ + + H DE + ++ + D V+ ++
Sbjct: 68 EVKEEEQLQEPKEENQDSVPLVEEVQDPIHADESENKICSVDQPTDDQVKPED 120
>At1g79400.1 68414.m09253 cation/proton exchanger, putative (CHX2)
monovalent cation:proton antiporter family 2 (CPA2)
member, PMID:11500563
Length = 783
Score = 29.1 bits (62), Expect = 1.4
Identities = 36/147 (24%), Positives = 62/147 (42%), Gaps = 6/147 (4%)
Frame = +2
Query: 20 VTQFVVMCGQTALVLIFMISVFGVKNNGNIVFVIMLTLLQGLCGMCFGFVIS----AACE 187
++ FVV + L+ I +FG+K + F+++L L I+ CE
Sbjct: 118 LSSFVVSGLLSFASLMLFIPLFGIKEDYFTFFLVLLVTLSNTASPVVVRSIADWKLNTCE 177
Query: 188 LERNAIQLALGSFYPTLLLSGVIWP-IEGMPWILRYVSLCLPLTLATNSLRSILTRGWPF 364
+ R I AL ++L +I I G I+ + L L T+A + +L P
Sbjct: 178 IGRLTISCALFIELTNVVLYTIIMAFISGT--IILELFLFLLATVALILINMVLAPWLPK 235
Query: 365 TD-SDVYMGFVSTLAWIALFLIVTLTI 442
+ + Y+ TL + LI+ +TI
Sbjct: 236 RNPKEKYLSKAETLVFFIFLLIIGITI 262
>At3g48740.1 68416.m05322 nodulin MtN3 family protein similar to
MtN3 GI:1619602 (root nodule development) from [Medicago
truncatula]
Length = 289
Score = 28.7 bits (61), Expect = 1.9
Identities = 24/88 (27%), Positives = 40/88 (45%), Gaps = 8/88 (9%)
Frame = +2
Query: 149 GMCFGF---VISAACELERNAIQLALGSFYP-----TLLLSGVIWPIEGMPWILRYVSLC 304
G+C GF V +A + R I+ + P TL +S VIW + G+ YV+
Sbjct: 137 GICVGFSVCVFAAPLSIIRTVIKTRSVEYMPFSLSLTLTISAVIWLLYGLALKDIYVAFP 196
Query: 305 LPLTLATNSLRSILTRGWPFTDSDVYMG 388
L A +L+ IL + + + ++G
Sbjct: 197 NVLGFALGALQMILYVVYKYCKTSPHLG 224
>At5g14180.1 68418.m01658 lipase family protein similar to SP|Q64194
Lysosomal acid lipase/cholesteryl ester hydrolase
precursor (EC 3.1.1.13) {Rattus norvegicus}; contains
Pfam profile PF04083: ab-hydrolase associated lipase
region
Length = 418
Score = 28.3 bits (60), Expect = 2.5
Identities = 19/66 (28%), Positives = 28/66 (42%), Gaps = 2/66 (3%)
Frame = +2
Query: 218 GSFYPTLLLSGVIWPIEGMPWILRYVSLCLPLTLATNSLRSIL--TRGWPFTDSDVYMGF 391
G P L+ G++ ++GM W+L LPL LA + TRG F+ Y+
Sbjct: 94 GKRQPVLIQHGIL--VDGMSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNP 151
Query: 392 VSTLAW 409
W
Sbjct: 152 SQRAFW 157
>At3g21220.1 68416.m02682 mitogen-activated protein kinase kinase
(MAPKK), putative (MKK5) identical to GB:BAA28831 from
[Arabidopsis thaliana]; mitogen-activated protein kinase
kinase (MAPKK) family, PMID:12119167
Length = 348
Score = 27.9 bits (59), Expect = 3.3
Identities = 15/46 (32%), Positives = 23/46 (50%)
Frame = +1
Query: 199 RDPAGLGFVLPHAAAERGDLAHRRDALDPALRVPLPAPHPRHELAA 336
+ P+G+ + + +R DL+ D AL VPLP P P +A
Sbjct: 5 QSPSGVASPMKNRLRKRPDLSLPLPHRDVALAVPLPLPPPSSSSSA 50
>At5g08580.1 68418.m01021 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 391
Score = 27.5 bits (58), Expect = 4.4
Identities = 11/48 (22%), Positives = 24/48 (50%)
Frame = -1
Query: 175 RYHEPEAHAAQTLQQSQHDNEDDVPIILDAEHRYHEDEHQRRLPAHHH 32
++H + ++ + ++HD P++ D E R + E +R+ H H
Sbjct: 39 QHHRLKLRSSFNFKPTRHDPVPFDPLVADMERRREDKEWERQYIEHSH 86
>At4g11720.1 68417.m01870 hypothetical protein
Length = 658
Score = 27.5 bits (58), Expect = 4.4
Identities = 11/29 (37%), Positives = 18/29 (62%), Gaps = 2/29 (6%)
Frame = -1
Query: 85 EHRYHEDEHQR--RLPAHHHELGDDVREQ 5
+HR+H + H+R + HHH DDV ++
Sbjct: 576 KHRHHHNHHRRTHQRHKHHHGQDDDVLQK 604
>At3g60620.1 68416.m06783 phosphatidate cytidylyltransferase family
protein contains Pfam profile: PF01148 phosphatidate
cytidylyltransferase
Length = 399
Score = 27.5 bits (58), Expect = 4.4
Identities = 21/81 (25%), Positives = 37/81 (45%), Gaps = 2/81 (2%)
Frame = +2
Query: 68 FMISVFGVKNNGNIVFVIMLTLLQGL--CGMCFGFVISAACELERNAIQLALGSFYPTLL 241
F++++ + GN F + + + GL CG F + C L + +G +PT+L
Sbjct: 199 FVVAMALLLQRGNPRFSQLSSTMFGLFYCGYLPCFWVKLRCGLTAPVLNTGIGRSWPTIL 258
Query: 242 LSGVIWPIEGMPWILRYVSLC 304
W + G+ IL +S C
Sbjct: 259 GGQAHWTV-GLVAIL--ISFC 276
>At2g45150.3 68415.m05621 phosphatidate cytidylyltransferase family
protein contains Pfam profile: PF01148 phosphatidate
cytidylyltransferase
Length = 382
Score = 27.1 bits (57), Expect = 5.8
Identities = 16/68 (23%), Positives = 30/68 (44%), Gaps = 2/68 (2%)
Frame = +2
Query: 68 FMISVFGVKNNGNIVFVIMLTLLQGL--CGMCFGFVISAACELERNAIQLALGSFYPTLL 241
F++++ + G+ F + + + GL CG F + C L A+ +G +P LL
Sbjct: 178 FVVAIALLVQRGSPRFAQLSSTMFGLFYCGYLPSFWVKLRCGLAAPALNTGIGRTWPILL 237
Query: 242 LSGVIWPI 265
W +
Sbjct: 238 GGQAHWTV 245
>At2g45150.2 68415.m05619 phosphatidate cytidylyltransferase family
protein contains Pfam profile: PF01148 phosphatidate
cytidylyltransferase
Length = 287
Score = 27.1 bits (57), Expect = 5.8
Identities = 16/68 (23%), Positives = 30/68 (44%), Gaps = 2/68 (2%)
Frame = +2
Query: 68 FMISVFGVKNNGNIVFVIMLTLLQGL--CGMCFGFVISAACELERNAIQLALGSFYPTLL 241
F++++ + G+ F + + + GL CG F + C L A+ +G +P LL
Sbjct: 187 FVVAIALLVQRGSPRFAQLSSTMFGLFYCGYLPSFWVKLRCGLAAPALNTGIGRTWPILL 246
Query: 242 LSGVIWPI 265
W +
Sbjct: 247 GGQAHWTV 254
>At2g45150.1 68415.m05620 phosphatidate cytidylyltransferase family
protein contains Pfam profile: PF01148 phosphatidate
cytidylyltransferase
Length = 391
Score = 27.1 bits (57), Expect = 5.8
Identities = 16/68 (23%), Positives = 30/68 (44%), Gaps = 2/68 (2%)
Frame = +2
Query: 68 FMISVFGVKNNGNIVFVIMLTLLQGL--CGMCFGFVISAACELERNAIQLALGSFYPTLL 241
F++++ + G+ F + + + GL CG F + C L A+ +G +P LL
Sbjct: 187 FVVAIALLVQRGSPRFAQLSSTMFGLFYCGYLPSFWVKLRCGLAAPALNTGIGRTWPILL 246
Query: 242 LSGVIWPI 265
W +
Sbjct: 247 GGQAHWTV 254
>At2g25870.1 68415.m03105 haloacid dehalogenase-like hydrolase
family protein contains Pfam profiles PF00702: haloacid
dehalogenase-like hydrolase, PF02130: Uncharacterized
protein family UPF0054
Length = 584
Score = 27.1 bits (57), Expect = 5.8
Identities = 14/50 (28%), Positives = 23/50 (46%)
Frame = -1
Query: 160 EAHAAQTLQQSQHDNEDDVPIILDAEHRYHEDEHQRRLPAHHHELGDDVR 11
E HA L SQH E +P+++ + + R+ H L D++R
Sbjct: 186 EDHATDVLSMSQHVPELKLPVLMMGDLVISVETAARQAAERGHTLLDEIR 235
>At1g24490.1 68414.m03084 60 kDa inner membrane family protein
similar to chloroplast membrane protein (ALBINO3)
(GI:3927828) [Arabidopsis thaliana]
Length = 1013
Score = 27.1 bits (57), Expect = 5.8
Identities = 18/58 (31%), Positives = 30/58 (51%)
Frame = +2
Query: 266 EGMPWILRYVSLCLPLTLATNSLRSILTRGWPFTDSDVYMGFVSTLAWIALFLIVTLT 439
EG WI SL P T+A S ++ +PF + +G+ TLA++ L L++ +
Sbjct: 737 EGFFWI---PSLAGPTTVAARQNGSGISWLFPFIEGHPPLGWPDTLAYLVLPLLLVFS 791
>At5g20580.2 68418.m02444 expressed protein predicted protein,
Arabidopsis thaliana
Length = 348
Score = 26.6 bits (56), Expect = 7.6
Identities = 13/47 (27%), Positives = 24/47 (51%)
Frame = +2
Query: 215 LGSFYPTLLLSGVIWPIEGMPWILRYVSLCLPLTLATNSLRSILTRG 355
+GS ++L+G G PWIL+ V +P L + ++ ++ G
Sbjct: 40 VGSCIYLIILAGYAILAAGAPWILQSVDYLIPSLLCSCNVALLMLTG 86
>At1g09830.1 68414.m01105 phosphoribosylamine--glycine ligase (PUR2)
Identical to phosphoribosylamine--glycine ligase,
chloroplast [precursor] SP:P52420 from [Arabidopsis
thaliana]
Length = 532
Score = 26.6 bits (56), Expect = 7.6
Identities = 16/54 (29%), Positives = 26/54 (48%)
Frame = -2
Query: 276 GIPSMGQITPLSSSVG*NEPKASWIAFRSSSQAADITNPKHMPHRPCSKVSMIT 115
G+ +G PL + + + KA + F SSQAA + K+ C K ++ T
Sbjct: 161 GLVVVGPEVPLVAGLANDLVKAGILTFGPSSQAAALEGSKNFMKNLCHKYNIPT 214
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,641,752
Number of Sequences: 28952
Number of extensions: 175872
Number of successful extensions: 742
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 709
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 735
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 722638680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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