BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0368 (567 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19G12.14 |its3||1-phosphatidylinositol-4-phosphate 5-kinase ... 107 8e-25 SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 60 2e-10 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 28 0.83 SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Pa... 27 2.5 SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr... 27 2.5 SPBC1347.07 |rex2||RNA exonuclease|Schizosaccharomyces pombe|chr... 26 3.4 SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 26 3.4 SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharo... 26 4.4 SPAC1F5.05c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 5.9 SPAC1783.04c |hst4||Sir2 family histone deacetylase Hst4|Schizos... 25 5.9 SPCC188.02 |par1||protein phosphatase regulatory subunit Par1 |S... 25 7.7 SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c... 25 7.7 >SPAC19G12.14 |its3||1-phosphatidylinositol-4-phosphate 5-kinase Its3|Schizosaccharomyces pombe|chr 1|||Manual Length = 742 Score = 107 bits (258), Expect = 8e-25 Identities = 56/163 (34%), Positives = 96/163 (58%), Gaps = 2/163 (1%) Frame = +2 Query: 83 DSSGKSGAKFYQSYDRLFILKTLTSEEVERMHSFLKHYHPYIVERHGKTLLPQYLGMYRL 262 DS GKSG+ FY S D FI+KT+ E + + L Y+ + V+ + TL+ Q+ G++R+ Sbjct: 359 DSPGKSGSFFYFSRDYRFIIKTIHHSEHKFLREILYDYYEH-VKNNPNTLISQFYGLHRV 417 Query: 263 TVD-GIEHYLVATRNVFSNHLNIHRKYDLKGSTVDREASXXXXXXXXP-TLKDNDFLKQG 436 + G + + V N+F H +IH+ +DLKGST+ RE T+KD +++++ Sbjct: 418 KLPFGRKIHFVVMNNLFPPHRDIHQTFDLKGSTLGRELDENQPCQSPMCTMKDTNWIRRN 477 Query: 437 VRIDIGEAAKEKLLETLTADVEFLTKLHLMDYSLLLGVHECGR 565 + + G ++ L + AD++ L+ L +MDYSLL+G+H+ R Sbjct: 478 MHLQFGPLKRQIFLTQVKADIDMLSSLGIMDYSLLVGIHDLSR 520 >SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase Fab1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1932 Score = 60.1 bits (139), Expect = 2e-10 Identities = 48/166 (28%), Positives = 78/166 (46%), Gaps = 9/166 (5%) Frame = +2 Query: 80 DDSSGKSGAKFYQSYDRLFILKTLTSEEVERMHSFLKHYHPYIVE---RHGKTLLPQYLG 250 + S GKSG+ F +++D+ +ILK L+ E + + +F Y YI + T L + G Sbjct: 1692 ESSGGKSGSAFLKTFDKKYILKVLSRLESDSLLNFAPAYFDYISKVFFHELPTALTKIFG 1751 Query: 251 MYRLTVDG------IEHYLVATRNVFSNHLNIHRKYDLKGSTVDREASXXXXXXXXPTLK 412 YR+ + + ++ NVF + R +DLKGS +R + Sbjct: 1752 FYRVDIRNPTTGTICKTDIMIMENVFYDECP-SRIFDLKGSMRNRHVESTGKVDEVLLDE 1810 Query: 413 DNDFLKQGVRIDIGEAAKEKLLETLTADVEFLTKLHLMDYSLLLGV 550 + L I + E K L L D FL+KL++MDYSL++G+ Sbjct: 1811 NLVELIYESPIFVSEQLKSLLHSCLWNDTLFLSKLNIMDYSLIVGI 1856 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 28.3 bits (60), Expect = 0.83 Identities = 22/76 (28%), Positives = 36/76 (47%), Gaps = 3/76 (3%) Frame = +2 Query: 122 YDRLFILKTLTSEEVERMHSFLKH-YHPYIVERHGK-TLLPQYLGMYRLT-VDGIEHYLV 292 Y F L++LTSE E FLK H Y ++ + ++ YL + L+ D +++ Sbjct: 376 YSVAFFLRSLTSENDELSSKFLKQIIHAYTHKQDNRLNIIQGYLDLVHLSDQDQYDNWFT 435 Query: 293 ATRNVFSNHLNIHRKY 340 +T + N RKY Sbjct: 436 STILTYLVIDNDQRKY 451 >SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Par2|Schizosaccharomyces pombe|chr 1|||Manual Length = 627 Score = 26.6 bits (56), Expect = 2.5 Identities = 15/40 (37%), Positives = 20/40 (50%) Frame = -3 Query: 229 GLAVPLHDVWMIVLQKRMHPLHLFRSQGLQDEQAIVALVE 110 G AVPL + I L K + PLH +S L Q +V+ Sbjct: 414 GFAVPLKEEHKIFLSKVLIPLHQTKSVFLYHPQLTYCIVQ 453 >SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr 2|||Manual Length = 1471 Score = 26.6 bits (56), Expect = 2.5 Identities = 11/29 (37%), Positives = 18/29 (62%) Frame = +2 Query: 221 GKTLLPQYLGMYRLTVDGIEHYLVATRNV 307 GKT+ +Y+ Y +V G++H V R+V Sbjct: 164 GKTVAAKYIMRYLTSVQGVDHNGVVKRSV 192 >SPBC1347.07 |rex2||RNA exonuclease|Schizosaccharomyces pombe|chr 2|||Manual Length = 180 Score = 26.2 bits (55), Expect = 3.4 Identities = 12/37 (32%), Positives = 21/37 (56%) Frame = +2 Query: 185 LKHYHPYIVERHGKTLLPQYLGMYRLTVDGIEHYLVA 295 L + + +E+HGK+ L + LTV +E+ L+A Sbjct: 55 LSEMNDWCIEQHGKSGLTERCRQSNLTVKDVENQLLA 91 >SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccharomyces pombe|chr 1|||Manual Length = 549 Score = 26.2 bits (55), Expect = 3.4 Identities = 13/56 (23%), Positives = 25/56 (44%) Frame = +2 Query: 158 EEVERMHSFLKHYHPYIVERHGKTLLPQYLGMYRLTVDGIEHYLVATRNVFSNHLN 325 + V R H+ K + Y++ H + L+P GI+ L+ ++ + LN Sbjct: 133 KNVRRTHAEFKKFAKYLISTHPECLIPAVPEAKTSVSSGIKEDLIYLKSGLQSWLN 188 >SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 860 Score = 25.8 bits (54), Expect = 4.4 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 2/41 (4%) Frame = +2 Query: 173 MHSFLKHYHPYIVERHGKTLL--PQYLGMYRLTVDGIEHYL 289 +H FL H+HP V K+L+ + LG L++ + H+L Sbjct: 593 IHPFLNHFHP-TVSLLAKSLVYGEKILGKPNLSLHTLNHFL 632 >SPAC1F5.05c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 25.4 bits (53), Expect = 5.9 Identities = 14/32 (43%), Positives = 21/32 (65%) Frame = -2 Query: 248 PSTAGAGSCRASPRCMDDSASETNASSPPLQK 153 PS A A+PR + S+S ++ASSPPL++ Sbjct: 73 PSNLKASYIPANPRRL--SSSTSSASSPPLRR 102 >SPAC1783.04c |hst4||Sir2 family histone deacetylase Hst4|Schizosaccharomyces pombe|chr 1|||Manual Length = 415 Score = 25.4 bits (53), Expect = 5.9 Identities = 10/20 (50%), Positives = 15/20 (75%) Frame = +2 Query: 179 SFLKHYHPYIVERHGKTLLP 238 SFLK ++P I +R+G T+ P Sbjct: 196 SFLKKFNPDIFDRNGVTVCP 215 >SPCC188.02 |par1||protein phosphatase regulatory subunit Par1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 548 Score = 25.0 bits (52), Expect = 7.7 Identities = 18/53 (33%), Positives = 26/53 (49%), Gaps = 3/53 (5%) Frame = -3 Query: 229 GLAVPLHDVWMIVLQKRMHPLHLFRSQGL---QDEQAIVALVELRTRLTGRIV 80 G A+PL + I L + + PLH +S L Q IV VE + +T +V Sbjct: 313 GFALPLKEEHKIFLSRVLIPLHKAKSLPLYYPQIAYGIVQFVEKDSSVTEEVV 365 >SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1111 Score = 25.0 bits (52), Expect = 7.7 Identities = 8/34 (23%), Positives = 21/34 (61%) Frame = +2 Query: 146 TLTSEEVERMHSFLKHYHPYIVERHGKTLLPQYL 247 +++ E + R+ ++++P+ + GK L+P +L Sbjct: 672 SISDEALARLCREFQYFYPFDIRASGKDLVPNHL 705 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,967,040 Number of Sequences: 5004 Number of extensions: 38689 Number of successful extensions: 130 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 126 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 127 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 240047038 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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