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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= P5PG0368
         (567 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC19G12.14 |its3||1-phosphatidylinositol-4-phosphate 5-kinase ...   107   8e-25
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho...    60   2e-10
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ...    28   0.83 
SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Pa...    27   2.5  
SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr...    27   2.5  
SPBC1347.07 |rex2||RNA exonuclease|Schizosaccharomyces pombe|chr...    26   3.4  
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha...    26   3.4  
SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharo...    26   4.4  
SPAC1F5.05c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    25   5.9  
SPAC1783.04c |hst4||Sir2 family histone deacetylase Hst4|Schizos...    25   5.9  
SPCC188.02 |par1||protein phosphatase regulatory subunit Par1 |S...    25   7.7  
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c...    25   7.7  

>SPAC19G12.14 |its3||1-phosphatidylinositol-4-phosphate 5-kinase
           Its3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 742

 Score =  107 bits (258), Expect = 8e-25
 Identities = 56/163 (34%), Positives = 96/163 (58%), Gaps = 2/163 (1%)
 Frame = +2

Query: 83  DSSGKSGAKFYQSYDRLFILKTLTSEEVERMHSFLKHYHPYIVERHGKTLLPQYLGMYRL 262
           DS GKSG+ FY S D  FI+KT+   E + +   L  Y+ + V+ +  TL+ Q+ G++R+
Sbjct: 359 DSPGKSGSFFYFSRDYRFIIKTIHHSEHKFLREILYDYYEH-VKNNPNTLISQFYGLHRV 417

Query: 263 TVD-GIEHYLVATRNVFSNHLNIHRKYDLKGSTVDREASXXXXXXXXP-TLKDNDFLKQG 436
            +  G + + V   N+F  H +IH+ +DLKGST+ RE            T+KD +++++ 
Sbjct: 418 KLPFGRKIHFVVMNNLFPPHRDIHQTFDLKGSTLGRELDENQPCQSPMCTMKDTNWIRRN 477

Query: 437 VRIDIGEAAKEKLLETLTADVEFLTKLHLMDYSLLLGVHECGR 565
           + +  G   ++  L  + AD++ L+ L +MDYSLL+G+H+  R
Sbjct: 478 MHLQFGPLKRQIFLTQVKADIDMLSSLGIMDYSLLVGIHDLSR 520


>SPBC3E7.01 |fab1|ste12,
            SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
            Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1932

 Score = 60.1 bits (139), Expect = 2e-10
 Identities = 48/166 (28%), Positives = 78/166 (46%), Gaps = 9/166 (5%)
 Frame = +2

Query: 80   DDSSGKSGAKFYQSYDRLFILKTLTSEEVERMHSFLKHYHPYIVE---RHGKTLLPQYLG 250
            + S GKSG+ F +++D+ +ILK L+  E + + +F   Y  YI +       T L +  G
Sbjct: 1692 ESSGGKSGSAFLKTFDKKYILKVLSRLESDSLLNFAPAYFDYISKVFFHELPTALTKIFG 1751

Query: 251  MYRLTVDG------IEHYLVATRNVFSNHLNIHRKYDLKGSTVDREASXXXXXXXXPTLK 412
             YR+ +         +  ++   NVF +     R +DLKGS  +R              +
Sbjct: 1752 FYRVDIRNPTTGTICKTDIMIMENVFYDECP-SRIFDLKGSMRNRHVESTGKVDEVLLDE 1810

Query: 413  DNDFLKQGVRIDIGEAAKEKLLETLTADVEFLTKLHLMDYSLLLGV 550
            +   L     I + E  K  L   L  D  FL+KL++MDYSL++G+
Sbjct: 1811 NLVELIYESPIFVSEQLKSLLHSCLWNDTLFLSKLNIMDYSLIVGI 1856


>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1044

 Score = 28.3 bits (60), Expect = 0.83
 Identities = 22/76 (28%), Positives = 36/76 (47%), Gaps = 3/76 (3%)
 Frame = +2

Query: 122 YDRLFILKTLTSEEVERMHSFLKH-YHPYIVERHGK-TLLPQYLGMYRLT-VDGIEHYLV 292
           Y   F L++LTSE  E    FLK   H Y  ++  +  ++  YL +  L+  D  +++  
Sbjct: 376 YSVAFFLRSLTSENDELSSKFLKQIIHAYTHKQDNRLNIIQGYLDLVHLSDQDQYDNWFT 435

Query: 293 ATRNVFSNHLNIHRKY 340
           +T   +    N  RKY
Sbjct: 436 STILTYLVIDNDQRKY 451


>SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit
           Par2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 627

 Score = 26.6 bits (56), Expect = 2.5
 Identities = 15/40 (37%), Positives = 20/40 (50%)
 Frame = -3

Query: 229 GLAVPLHDVWMIVLQKRMHPLHLFRSQGLQDEQAIVALVE 110
           G AVPL +   I L K + PLH  +S  L   Q    +V+
Sbjct: 414 GFAVPLKEEHKIFLSKVLIPLHQTKSVFLYHPQLTYCIVQ 453


>SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1471

 Score = 26.6 bits (56), Expect = 2.5
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +2

Query: 221 GKTLLPQYLGMYRLTVDGIEHYLVATRNV 307
           GKT+  +Y+  Y  +V G++H  V  R+V
Sbjct: 164 GKTVAAKYIMRYLTSVQGVDHNGVVKRSV 192


>SPBC1347.07 |rex2||RNA exonuclease|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 180

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 12/37 (32%), Positives = 21/37 (56%)
 Frame = +2

Query: 185 LKHYHPYIVERHGKTLLPQYLGMYRLTVDGIEHYLVA 295
           L   + + +E+HGK+ L +      LTV  +E+ L+A
Sbjct: 55  LSEMNDWCIEQHGKSGLTERCRQSNLTVKDVENQLLA 91


>SPAPJ696.01c |vps17||retromer complex subunit
           Vps17|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 549

 Score = 26.2 bits (55), Expect = 3.4
 Identities = 13/56 (23%), Positives = 25/56 (44%)
 Frame = +2

Query: 158 EEVERMHSFLKHYHPYIVERHGKTLLPQYLGMYRLTVDGIEHYLVATRNVFSNHLN 325
           + V R H+  K +  Y++  H + L+P           GI+  L+  ++   + LN
Sbjct: 133 KNVRRTHAEFKKFAKYLISTHPECLIPAVPEAKTSVSSGIKEDLIYLKSGLQSWLN 188


>SPAC4F10.09c |||ribosome biogenesis protein Noc1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 860

 Score = 25.8 bits (54), Expect = 4.4
 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 2/41 (4%)
 Frame = +2

Query: 173 MHSFLKHYHPYIVERHGKTLL--PQYLGMYRLTVDGIEHYL 289
           +H FL H+HP  V    K+L+   + LG   L++  + H+L
Sbjct: 593 IHPFLNHFHP-TVSLLAKSLVYGEKILGKPNLSLHTLNHFL 632


>SPAC1F5.05c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 151

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 14/32 (43%), Positives = 21/32 (65%)
 Frame = -2

Query: 248 PSTAGAGSCRASPRCMDDSASETNASSPPLQK 153
           PS   A    A+PR +  S+S ++ASSPPL++
Sbjct: 73  PSNLKASYIPANPRRL--SSSTSSASSPPLRR 102


>SPAC1783.04c |hst4||Sir2 family histone deacetylase
           Hst4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 415

 Score = 25.4 bits (53), Expect = 5.9
 Identities = 10/20 (50%), Positives = 15/20 (75%)
 Frame = +2

Query: 179 SFLKHYHPYIVERHGKTLLP 238
           SFLK ++P I +R+G T+ P
Sbjct: 196 SFLKKFNPDIFDRNGVTVCP 215


>SPCC188.02 |par1||protein phosphatase regulatory subunit Par1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 548

 Score = 25.0 bits (52), Expect = 7.7
 Identities = 18/53 (33%), Positives = 26/53 (49%), Gaps = 3/53 (5%)
 Frame = -3

Query: 229 GLAVPLHDVWMIVLQKRMHPLHLFRSQGL---QDEQAIVALVELRTRLTGRIV 80
           G A+PL +   I L + + PLH  +S  L   Q    IV  VE  + +T  +V
Sbjct: 313 GFALPLKEEHKIFLSRVLIPLHKAKSLPLYYPQIAYGIVQFVEKDSSVTEEVV 365


>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1111

 Score = 25.0 bits (52), Expect = 7.7
 Identities = 8/34 (23%), Positives = 21/34 (61%)
 Frame = +2

Query: 146 TLTSEEVERMHSFLKHYHPYIVERHGKTLLPQYL 247
           +++ E + R+    ++++P+ +   GK L+P +L
Sbjct: 672 SISDEALARLCREFQYFYPFDIRASGKDLVPNHL 705


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,967,040
Number of Sequences: 5004
Number of extensions: 38689
Number of successful extensions: 130
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 240047038
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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