BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0315 (626 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 30 0.24 SPAP14E8.03 |bos1||SNARE Bos1|Schizosaccharomyces pombe|chr 1|||... 28 0.96 SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 25 6.8 >SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 30.3 bits (65), Expect = 0.24 Identities = 18/53 (33%), Positives = 27/53 (50%) Frame = +3 Query: 459 YIQTILGFLTLQV*L*KCLHYKTLSF*FYEVMNNS*IYKQLRRVKVSPNKNRN 617 +I +LG + + + L CLH+ FY ++N I Q R V + PNK N Sbjct: 384 FIGVLLGLIGV-IGLFICLHFSGAMNGFYRLLNRHGIPVQERIVNIGPNKPEN 435 >SPAP14E8.03 |bos1||SNARE Bos1|Schizosaccharomyces pombe|chr 1|||Manual Length = 235 Score = 28.3 bits (60), Expect = 0.96 Identities = 15/40 (37%), Positives = 26/40 (65%), Gaps = 1/40 (2%) Frame = +1 Query: 247 LSLSKSPIDNYGCQSDRQLTSATSR-IYLRVEKWKTKHLQ 363 LSLS+S ID+Y R+L A + +R+++++ KH+Q Sbjct: 48 LSLSRS-IDDYDSMVQRELVPAKKKKATIRIQEFRQKHVQ 86 >SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 1207 Score = 25.4 bits (53), Expect = 6.8 Identities = 13/47 (27%), Positives = 23/47 (48%) Frame = +2 Query: 254 CQSHPLTITAVRVTDSSRQQQVEFISGSKNGKQNICKKHFHTNSCVL 394 C HP T+ + R ++ ++ SK + + C+ HF+ SC L Sbjct: 218 CAFHP-TLPLILSAGDDRLVKLWRMTASKAWEVDTCRGHFNNVSCCL 263 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,215,650 Number of Sequences: 5004 Number of extensions: 40164 Number of successful extensions: 100 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 98 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 100 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 277683324 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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