Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0308
(640 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 157 2e-39
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 156 2e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 87 2e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 66 4e-12
SPCC1450.14c |ero12||ER oxidoreductin Ero1b|Schizosaccharomyces ... 27 2.3
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 3.0
SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c... 26 5.3
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.3
SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 25 7.0
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.2
SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10 |Schizos... 25 9.2
SPBC3E7.02c |hsp16||heat shock protein Hsp16|Schizosaccharomyces... 25 9.2
SPAC22H10.07 |scd2|ral3|scaffold protein Scd2|Schizosaccharomyce... 25 9.2
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 157 bits (380), Expect = 2e-39
Identities = 73/132 (55%), Positives = 94/132 (71%)
Frame = +1
Query: 244 PQTRPSGVETILSTLSSARPELASTYPGAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGK 423
P+T + ST S + P +++VDLEP V+D+VRTG YR LFHPEQLITGK
Sbjct: 38 PETASQNSDGGFSTFFSETGQ-GKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGK 96
Query: 424 EDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMER 603
EDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH LL+ER
Sbjct: 97 EDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLER 156
Query: 604 LSVDYGKKSKLE 639
L+++Y KKSKL+
Sbjct: 157 LAMEYTKKSKLQ 168
Score = 104 bits (249), Expect = 1e-23
Identities = 45/64 (70%), Positives = 50/64 (78%)
Frame = +3
Query: 135 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 314
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 315 HVPR 326
+VPR
Sbjct: 61 YVPR 64
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 156 bits (379), Expect = 2e-39
Identities = 65/106 (61%), Positives = 87/106 (82%)
Frame = +1
Query: 322 PGAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 501
P +++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RI
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 502 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLE 639
R++AD C+GLQGFL+FH LL+ERL+++YGKKS L+
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQ 172
Score = 97.1 bits (231), Expect = 2e-21
Identities = 49/69 (71%), Positives = 51/69 (73%), Gaps = 5/69 (7%)
Frame = +3
Query: 135 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 299
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 300 TGAGKHVPR 326
TG GK VPR
Sbjct: 60 TGQGKFVPR 68
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 87.0 bits (206), Expect = 2e-18
Identities = 40/99 (40%), Positives = 58/99 (58%)
Frame = +1
Query: 322 PGAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 501
P AV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 502 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDY 618
R+ A+ C LQGF + H LL+ ++ +Y
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEY 159
Score = 47.6 bits (108), Expect = 2e-06
Identities = 24/67 (35%), Positives = 32/67 (47%)
Frame = +3
Query: 135 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 314
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 315 HVPRCCL 335
+VPR L
Sbjct: 59 YVPRAVL 65
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 66.1 bits (154), Expect = 4e-12
Identities = 36/104 (34%), Positives = 56/104 (53%), Gaps = 2/104 (1%)
Frame = +1
Query: 322 PGAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLD 495
P A+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D
Sbjct: 62 PRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMD 120
Query: 496 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKK 627
I + AD L+GF + H L+ERL+ Y KK
Sbjct: 121 MIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKK 164
Score = 55.6 bits (128), Expect = 6e-09
Identities = 27/66 (40%), Positives = 39/66 (59%)
Frame = +3
Query: 138 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 317
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 318 VPRCCL 335
+PR L
Sbjct: 61 IPRAIL 66
>SPCC1450.14c |ero12||ER oxidoreductin Ero1b|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 571
Score = 27.1 bits (57), Expect = 2.3
Identities = 14/33 (42%), Positives = 22/33 (66%)
Frame = -3
Query: 149 DALTHFDSTSFSMLKCRIKL*SVVVQRDRLSGS 51
D +T+FDS SFS+ R ++ +++ DRLS S
Sbjct: 422 DEVTNFDSRSFSLRLRRSEIVALINTFDRLSRS 454
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.6 bits (56), Expect = 3.0
Identities = 21/65 (32%), Positives = 30/65 (46%)
Frame = +1
Query: 124 VESKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCPQTRPSGVETILSTLSSARP 303
V S + +S S S+ + T+ ST SSL + ++PS T ST SSA P
Sbjct: 144 VSSAILPSSTSVEVSISSSSLSSSDPLTS-STFSSLSSSTSSSQPSVSSTSSSTFSSAAP 202
Query: 304 ELAST 318
S+
Sbjct: 203 TSTSS 207
>SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 649
Score = 25.8 bits (54), Expect = 5.3
Identities = 19/77 (24%), Positives = 35/77 (45%), Gaps = 3/77 (3%)
Frame = +1
Query: 268 ETILSTLSSARPELASTYPGAVFVDLE-PTVVDEVRTGTYRQLFHPEQLITG--KEDAAN 438
ET+L ++ + +A + A+ + L + + Y + E+ I +E AN
Sbjct: 545 ETLLLSVVRGKSGIAFLFFMAIMLKLLLGKAILAITQSRYESMQQREEKINSWERERKAN 604
Query: 439 NYARGHYTIGKEIVDLV 489
N+ RGH I K+ D +
Sbjct: 605 NFFRGHIEIDKKTKDFL 621
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 5.3
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 233 WPDAHRQDHRGWRRFFQHFLQR 298
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 317
Score = 25.4 bits (53), Expect = 7.0
Identities = 24/83 (28%), Positives = 36/83 (43%)
Frame = +1
Query: 130 SKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCPQTRPSGVETILSTLSSARPEL 309
SK S+S T P S S A A TAS+ + + S V +++S+ SSA
Sbjct: 219 SKFSSSSFITSTTPASSSSSGAIVSNA-KTASTDDSSSASSATSSVSSVVSSASSALSAS 277
Query: 310 ASTYPGAVFVDLEPTVVDEVRTG 378
AS+ +V ++TG
Sbjct: 278 ASSASASVSSSASSDASPALKTG 300
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.0 bits (52), Expect = 9.2
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +1
Query: 157 TLAKPESRSVMPAGSFTAWSTASSLMARCP 246
+L+ P SR++ P S + STASSL P
Sbjct: 181 SLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1649
Score = 25.0 bits (52), Expect = 9.2
Identities = 15/36 (41%), Positives = 18/36 (50%)
Frame = -1
Query: 169 AWPTCTEMHSRILIRLPFLCLNVELNYNQSSFSATG 62
AWP C + S +RL L +EL SS SA G
Sbjct: 1349 AWPECARLGSTAALRLLEL---IELALQNSSRSAIG 1381
>SPBC3E7.02c |hsp16||heat shock protein Hsp16|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 143
Score = 25.0 bits (52), Expect = 9.2
Identities = 22/76 (28%), Positives = 31/76 (40%)
Frame = +1
Query: 256 PSGVETILSTLSSARPELASTYPGAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAA 435
P V + S S P L + PG +L P++ +V G E KED
Sbjct: 10 PPTVNDLFSDFVSYSPRLNNQIPG----ELSPSI--DVHEGKDTVSVDVELPGVKKEDVQ 63
Query: 436 NNYARGHYTIGKEIVD 483
+Y G TI E+V+
Sbjct: 64 VHYDSGKLTISGEVVN 79
>SPAC22H10.07 |scd2|ral3|scaffold protein Scd2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 536
Score = 25.0 bits (52), Expect = 9.2
Identities = 18/68 (26%), Positives = 33/68 (48%)
Frame = +1
Query: 178 RSVMPAGSFTAWSTASSLMARCPQTRPSGVETILSTLSSARPELASTYPGAVFVDLEPTV 357
+S +P G F+ T ++ + P T + ++ A + +ST+PG+V +L P
Sbjct: 219 KSSIPLGKFSDGET-QTMPSLSPSTENLQINNDVTY--QAATDNSSTFPGSVANELTPLQ 275
Query: 358 VDEVRTGT 381
E RT +
Sbjct: 276 TLESRTAS 283
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,703,352
Number of Sequences: 5004
Number of extensions: 55914
Number of successful extensions: 170
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 167
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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