Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0304
(637 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 97 1e-20
At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 93 1e-19
At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 93 2e-19
At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 89 2e-18
At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 87 7e-18
At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 87 9e-18
At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 87 1e-17
At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 86 2e-17
At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 81 8e-16
At5g20270.1 68418.m02413 expressed protein contains Pfam domain,... 28 4.5
At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox... 28 6.0
At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase fam... 28 6.0
At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory /... 28 6.0
>At3g15870.1 68416.m02007 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 292
Score = 96.7 bits (230), Expect = 1e-20
Identities = 54/172 (31%), Positives = 83/172 (48%)
Frame = +3
Query: 105 LFFLGNFGITAGAHRLWSHNGYKVKLPLEILLMVFNSIAFQNTIFTWVRDHRLHHKYTDT 284
L F+ IT HR SH + + LE L +AFQ WV +HR HHK+ +T
Sbjct: 58 LVFINGICITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCET 117
Query: 285 DADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLRFQKKYAIPFIGTIC 464
DPH+ T+GF+FSH+ W+ + G+ ++ D+ P RF ++ + +
Sbjct: 118 QRDPHSPTQGFWFSHMAWIFDTSSILENCGGEE-NVDDLVRQPFYRFLQRTVLLHMMAYS 176
Query: 465 FIIPTLAPMYFWGESLNNAWHITVLRYIFSLNGTFLVNSAAHLWGYKPYDKS 620
F+ YF G W I + + L+ TFLVNS H+WG + ++ S
Sbjct: 177 FL------FYFCGGMPLLVWGIGITIAV-RLHLTFLVNSVCHIWGTRAWNTS 221
>At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to
delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from
[Arabidopsis thaliana]
Length = 307
Score = 93.1 bits (221), Expect = 1e-19
Identities = 56/172 (32%), Positives = 85/172 (49%), Gaps = 3/172 (1%)
Frame = +3
Query: 105 LFF-LGNFGITAGAHRLWSHNGYKVKLPLEILLMVFNSIAFQNTIFTWVRDHRLHHKYTD 281
LF+ +G GIT HR +H +KV LE LL +A Q WV HR HH++TD
Sbjct: 71 LFYTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTD 130
Query: 282 TDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLRFQKKYAIPFIGTI 461
++ DPH+ GF+FSH+ W+ + +V G+ ++ D+ RF +K + I +
Sbjct: 131 SERDPHSPKEGFWFSHLLWIYDSAY-LVSKCGRRANVEDLKRQWFYRFLQKTVLFHILGL 189
Query: 462 CFIIPTLAPMYF--WGESLNNAWHITVLRYIFSLNGTFLVNSAAHLWGYKPY 611
F + L M F WG + A + V T L+NS H+WG + +
Sbjct: 190 GFFLFYLGGMSFVTWGMGVGAALEVHV---------TCLINSLCHIWGTRTW 232
>At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to
delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180
GI:2970034 from [Arabidopsis thaliana]
Length = 305
Score = 92.7 bits (220), Expect = 2e-19
Identities = 50/172 (29%), Positives = 86/172 (50%)
Frame = +3
Query: 96 AIVLFFLGNFGITAGAHRLWSHNGYKVKLPLEILLMVFNSIAFQNTIFTWVRDHRLHHKY 275
A++++ +G GIT HR +H +KV LE +A Q WV HR HH++
Sbjct: 67 ALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQF 126
Query: 276 TDTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLRFQKKYAIPFIG 455
TD+D DPH+ GF+FSH+ WL + +V+ G+ ++ D+ +F ++ + I
Sbjct: 127 TDSDRDPHSPNEGFWFSHLLWLFDTGY-LVEKCGRRTNVEDLKRQWYYKFLQRTVLYHIL 185
Query: 456 TICFIIPTLAPMYFWGESLNNAWHITVLRYIFSLNGTFLVNSAAHLWGYKPY 611
T F+ +Y++G W + + + T L+NS H+WG + +
Sbjct: 186 TFGFL------LYYFGGLSFLTWGMGI-GVAMEHHVTCLINSLCHVWGSRTW 230
>At3g15850.1 68416.m02005 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 371
Score = 89.0 bits (211), Expect = 2e-18
Identities = 51/168 (30%), Positives = 79/168 (47%), Gaps = 2/168 (1%)
Frame = +3
Query: 117 GNFGITAGAHRLWSHNGYKVKLPLEILLMVFNSIAFQNTIFTWVRDHRLHHKYTDTDADP 296
G GIT HR SH +K+ LE L + A Q WV HR HH++ D+D DP
Sbjct: 139 GLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDP 198
Query: 297 HNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLRFQKKYAI--PFIGTICFI 470
H+ GF+FSH+ W+ + + + G+ ++ D+ P RF + I P +
Sbjct: 199 HSPLDGFWFSHMNWMF-DTNTITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLALAVALY 257
Query: 471 IPTLAPMYFWGESLNNAWHITVLRYIFSLNGTFLVNSAAHLWGYKPYD 614
P WG + W ++ + T+LVNSA H+WG + ++
Sbjct: 258 AMGGFPFIVWGMGVRIVW-------VYHI--TWLVNSACHVWGKQAWN 296
>At1g06360.1 68414.m00672 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 87.4 bits (207), Expect = 7e-18
Identities = 51/172 (29%), Positives = 82/172 (47%), Gaps = 2/172 (1%)
Frame = +3
Query: 102 VLFFLGNFGITAGAHRLWSHNGYKVKLPLEILLMVFNSIAFQNTIFTWVRDHRLHHKYTD 281
VL+ L + IT HR +H +K+ LE L F A Q WV HR HH++TD
Sbjct: 63 VLYALTSLSITFSYHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDWVSIHRFHHQFTD 122
Query: 282 TDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKS--LDMSDIYCNPLLRFQKKYAIPFIG 455
+D DPH+ GF+FSH+ W+ ++ K G++ +D+ + LR + +
Sbjct: 123 SDRDPHSPIEGFWFSHVWWICDTRYIKYKCGGRNNVMDLKQQWFYWFLRMTIGFHVLMFW 182
Query: 456 TICFIIPTLAPMYFWGESLNNAWHITVLRYIFSLNGTFLVNSAAHLWGYKPY 611
T+ ++ L P G + + + T+LVNSA H+WG + +
Sbjct: 183 TVLYLYGGL-PYLTCGGGVGG---------VIGYHVTWLVNSACHIWGSRSW 224
>At1g06100.1 68414.m00639 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 87.0 bits (206), Expect = 9e-18
Identities = 54/167 (32%), Positives = 78/167 (46%)
Frame = +3
Query: 99 IVLFFLGNFGITAGAHRLWSHNGYKVKLPLEILLMVFNSIAFQNTIFTWVRDHRLHHKYT 278
I+L L N IT HR +H +K+ LE +A Q WV HR HH++T
Sbjct: 62 IILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFT 121
Query: 279 DTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLRFQKKYAIPFIGT 458
D+D DPH+ GF+FSH+ W+ + K G+ ++ D+ RF KK + I
Sbjct: 122 DSDRDPHSPIEGFWFSHVLWIFDTDYIREK-CGRRNNVMDLKQQWFYRFLKKTLVLHILA 180
Query: 459 ICFIIPTLAPMYFWGESLNNAWHITVLRYIFSLNGTFLVNSAAHLWG 599
+I Y WG W + + +GT+LVNSA H+ G
Sbjct: 181 FWTLI------YLWGGLPYLTWTVG-FGGVIGYHGTWLVNSACHICG 220
>At1g06090.1 68414.m00638 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]
Length = 299
Score = 86.6 bits (205), Expect = 1e-17
Identities = 53/172 (30%), Positives = 81/172 (47%)
Frame = +3
Query: 99 IVLFFLGNFGITAGAHRLWSHNGYKVKLPLEILLMVFNSIAFQNTIFTWVRDHRLHHKYT 278
++L + + IT HR +H +K+ LE A Q WV HR HH++T
Sbjct: 62 VILAIVTSLSITFSYHRNLTHKSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFT 121
Query: 279 DTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLRFQKKYAIPFIGT 458
D+D DPH+ GF+FSH+ W+ + K G+ ++ D+ RF + IG
Sbjct: 122 DSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRFLRN----TIGL 176
Query: 459 ICFIIPTLAPMYFWGESLNNAWHITVLRYIFSLNGTFLVNSAAHLWGYKPYD 614
TL +Y WG + V I NGT+L+NSA H+WG + ++
Sbjct: 177 HILTFWTL--VYLWGGLPYLTCGVGVGGTI-GYNGTWLINSACHIWGSRAWN 225
>At1g06120.1 68414.m00641 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported
by cDNA:gi_12083275_gb_AF332434.1_AF332434
Length = 299
Score = 85.8 bits (203), Expect = 2e-17
Identities = 50/165 (30%), Positives = 76/165 (46%)
Frame = +3
Query: 120 NFGITAGAHRLWSHNGYKVKLPLEILLMVFNSIAFQNTIFTWVRDHRLHHKYTDTDADPH 299
N IT HR +H +K+ LE A Q WV HR HH++TD+D DPH
Sbjct: 69 NLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDPH 128
Query: 300 NATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLRFQKKYAIPFIGTICFIIPT 479
+ GF+FSH+ W+ + K G+ ++ D+ RF + I T ++
Sbjct: 129 SPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRFLQNTIGLHILTFWILV-- 185
Query: 480 LAPMYFWGESLNNAWHITVLRYIFSLNGTFLVNSAAHLWGYKPYD 614
Y WG W + V I + T+L+NSA H+WG + ++
Sbjct: 186 ----YLWGGLPYLTWSVGVGGAI-GYHATWLINSACHIWGSRAWN 225
>At1g06350.1 68414.m00671 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 300
Score = 80.6 bits (190), Expect = 8e-16
Identities = 54/174 (31%), Positives = 76/174 (43%), Gaps = 2/174 (1%)
Frame = +3
Query: 99 IVLFFLGNFGITAGAHRLWSHNGYKVKLPLEILLMVFNSIAFQNTIFTWVRDHRLHHKYT 278
+VLF L IT HR SH +K+ LE A Q WV HR HH++T
Sbjct: 63 LVLFALTTLSITFSFHRNLSHRSFKIPKWLEYPWAYSAVFALQGDPMDWVSIHRFHHQFT 122
Query: 279 DTDADPHNATRGFFFSHIGWLLVRKHPMVKIAGKS--LDMSDIYCNPLLRFQKKYAIPFI 452
D+D DPH+ G FSHI W+ ++ K G+ LD+ + LR I
Sbjct: 123 DSDRDPHSPKEGLLFSHILWIFDTQYIKYKCGGRDNVLDLKKQWFYKFLRRTIAVHILMF 182
Query: 453 GTICFIIPTLAPMYFWGESLNNAWHITVLRYIFSLNGTFLVNSAAHLWGYKPYD 614
TI ++ L P G + + T+LVNSA H+WG + ++
Sbjct: 183 WTILYLYGGL-PYLTCGGGVG---------IFIGYHVTWLVNSACHIWGSRSWN 226
>At5g20270.1 68418.m02413 expressed protein contains Pfam domain,
PF03006: Uncharacterised protein family (Hly-III /
UPF0073)
Length = 332
Score = 28.3 bits (60), Expect = 4.5
Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 3/61 (4%)
Frame = +3
Query: 36 WSIWTLSLFHLSKIGYIGCCAIVLFFLGNFGITAGAHRL---WSHNGYKVKLPLEILLMV 206
++I TL LS Y A++ +G FGI AH L W + V L E+L+ V
Sbjct: 214 FTIITLFTPSLSAPKYRAFRALLFASMGLFGIVPAAHALVVNWGNPQRNVTLVYELLMAV 273
Query: 207 F 209
F
Sbjct: 274 F 274
>At1g72520.1 68414.m08386 lipoxygenase, putative similar to
lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140
[Lycopersicon esculentum], GB:CAB56692 [Arabidopsis
thaliana]
Length = 926
Score = 27.9 bits (59), Expect = 6.0
Identities = 13/47 (27%), Positives = 17/47 (36%)
Frame = +2
Query: 194 PVDGLQQYCFSEHHFHMGERSQATSQVYGH*CRPS*CYERFFFLTHR 334
PVD + + H+G Q+ H R C E F HR
Sbjct: 556 PVDATSNWMWQLAKAHVGSNDAGVHQLVNHWLRTHACLEPFILAAHR 602
>At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase family
protein low similarity to dTDP-D-glucose-4,6-dehydratase
from Aneurinibacillus thermoaerophilus GI:16357461,
Sphingomonas sp. GI:1314581; contains Pfam profile
PF01370: NAD dependent epimerase/dehydratase family;
putative NDP-rhamnose synthase (rhm2 gene) GI:31559258
Length = 667
Score = 27.9 bits (59), Expect = 6.0
Identities = 20/60 (33%), Positives = 26/60 (43%), Gaps = 4/60 (6%)
Frame = +3
Query: 279 DTDADPHN----ATRGFFFSHIGWLLVRKHPMVKIAGKSLDMSDIYCNPLLRFQKKYAIP 446
DT P N GF SH+ L+R +P KI LD D YC+ L ++ P
Sbjct: 3 DTTYKPKNILITGAAGFIASHVANRLIRNYPDYKIV--VLDKLD-YCSDLKNLDPSFSSP 59
>At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory / CAF
identical to RNA helicase/RNAseIII CAF protein
GB:AAF03534 GI:6102610 from [Arabidopsis thaliana]
Length = 1909
Score = 27.9 bits (59), Expect = 6.0
Identities = 21/58 (36%), Positives = 29/58 (50%)
Frame = -1
Query: 544 YLKTVICQALFKLSPQKYIGANVGMIKHIVPINGIAYFF*NLKRGLQ*ISDMSRLFPA 371
YLK V+ + LF PQK+ G M + +V N + Y F L +GLQ R P+
Sbjct: 1384 YLKWVVSRFLFLKYPQKHEGQLTRMRQQMVS-NMVLYQF-ALVKGLQSYIQADRFAPS 1439
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,406,860
Number of Sequences: 28952
Number of extensions: 307702
Number of successful extensions: 720
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 699
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 711
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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