Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= P5PG0287
         (458 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA...   189   9e-49
At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA...   187   3e-48
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA...   186   5e-48
At5g14460.1 68418.m01692 pseudouridylate synthase TruB family pr...    31   0.28 
At1g15870.1 68414.m01904 mitochondrial glycoprotein family prote...    31   0.28 
At5g56100.1 68418.m06999 glycine-rich protein / oleosin contains...    29   2.0  
At5g08390.1 68418.m00988 transducin family protein / WD-40 repea...    28   3.5  
At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138...    28   3.5  
At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50...    27   8.1  
At1g12300.1 68414.m01422 pentatricopeptide (PPR) repeat-containi...    27   8.1  

>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
           limon]; Nicotiana tabacum SP|P50218
          Length = 416

 Score =  189 bits (460), Expect = 9e-49
 Identities = 87/122 (71%), Positives = 101/122 (82%), Gaps = 1/122 (0%)
 Frame = +1

Query: 91  KIKA-GPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 267
           KIK   PVV++ GDEMTR+IW  IK+KLI PFL++++  +DLG+ NRD TDD+VTI+ AE
Sbjct: 5   KIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETAE 64

Query: 268 AIKKYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVT 447
           A  KYNV IKCATITPDE RV EF LKKMW+SPNGTIRNIL GTVFRE IIC+NIPRLV 
Sbjct: 65  ATLKYNVAIKCATITPDEARVREFGLKKMWRSPNGTIRNILNGTVFREPIICRNIPRLVP 124

Query: 448 GW 453
           GW
Sbjct: 125 GW 126


>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
           GI:3021512; contains Pfam domain PF00180: dehydrogenase,
           isocitrate/isopropylmalate family
          Length = 485

 Score =  187 bits (456), Expect = 3e-48
 Identities = 82/117 (70%), Positives = 100/117 (85%)
 Frame = +1

Query: 106 PVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYN 285
           P+V++ GDEMTR+IW +IKEKLILP+LD+++  +DLG+ NRD TDD+VT++ AEA  KYN
Sbjct: 81  PIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAEAALKYN 140

Query: 286 VGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWD 456
           V IKCATITPDE RV+EF LK MW+SPNGTIRNIL GTVFRE I+C NIPRLV GW+
Sbjct: 141 VAIKCATITPDEGRVKEFGLKSMWRSPNGTIRNILDGTVFREPIMCSNIPRLVPGWE 197


>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase SP|Q40345 from [Medicago
           sativa]
          Length = 410

 Score =  186 bits (454), Expect = 5e-48
 Identities = 84/122 (68%), Positives = 103/122 (84%), Gaps = 1/122 (0%)
 Frame = +1

Query: 91  KIK-AGPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 267
           KIK A P+V++ GDEMTR+IW  IK+KLI PF+++++  +DLG+ +RD TDD+VTI+ AE
Sbjct: 5   KIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESAE 64

Query: 268 AIKKYNVGIKCATITPDEKRVEEFKLKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVT 447
           A KKYNV IKCATITPDE RV EF LK+MW+SPNGTIRNIL GTVFRE IICKN+P+LV 
Sbjct: 65  ATKKYNVAIKCATITPDEGRVTEFGLKQMWRSPNGTIRNILNGTVFREPIICKNVPKLVP 124

Query: 448 GW 453
           GW
Sbjct: 125 GW 126


>At5g14460.1 68418.m01692 pseudouridylate synthase TruB family
           protein similar to SP|P09171 tRNA pseudouridine synthase
           B (EC 4.2.1.70) (tRNA pseudouridine 55 synthase)
           (Pseudouridylate synthase) (Uracil hydrolyase)
           {Escherichia coli O157:H7}; contains Pfam profile
           PF01509: TruB family pseudouridylate synthase (N
           terminal domain)
          Length = 540

 Score = 31.5 bits (68), Expect = 0.28
 Identities = 21/70 (30%), Positives = 34/70 (48%)
 Frame = +1

Query: 160 KEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYNVGIKCATITPDEKRVEEF 339
           K+K++         V  +GMEN D+ DD++        K+     +   +   EK+VE+F
Sbjct: 222 KKKVVAALGGKRSEVKRIGMENCDEDDDKLVFFDEVKEKEEKKKSEDDVVVVTEKKVEQF 281

Query: 340 KLKKMWKSPN 369
             K + KSPN
Sbjct: 282 -FKGLTKSPN 290


>At1g15870.1 68414.m01904 mitochondrial glycoprotein family protein
           / MAM33 family protein similar to SUAPRGA1 [Emericella
           nidulans] GI:6562379; contains Pfam profile PF02330:
           Mitochondrial glycoprotein
          Length = 242

 Score = 31.5 bits (68), Expect = 0.28
 Identities = 22/83 (26%), Positives = 37/83 (44%)
 Frame = +1

Query: 34  PTNLKSTRLFIEVERYNMSKIKAGPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDL 213
           P  ++ T+ F+     N      GP    + DE+   ++  ++E+ I   L   LH Y  
Sbjct: 162 PDTIQITKFFVRKSSQNSPNAYIGPEFQEMEDELQDSVYRFLEERGISDDLAEFLHQY-- 219

Query: 214 GMENRDKTDDQVTIDCAEAIKKY 282
            M+N+DK +    I   E +K Y
Sbjct: 220 -MKNKDKAE---YIRWMETVKSY 238


>At5g56100.1 68418.m06999 glycine-rich protein / oleosin contains
           Pfam PF01277: Oleosin domain
          Length = 150

 Score = 28.7 bits (61), Expect = 2.0
 Identities = 12/39 (30%), Positives = 24/39 (61%)
 Frame = -1

Query: 179 GRISFSLIRSQIILVISSPRMSTTGPAFILDILYLSTSM 63
           G +SFS + +  + +I+SP +    PAF++ +  L ++M
Sbjct: 41  GLMSFSFVATVTLFLIASPLLLIFAPAFMVTVAVLVSAM 79


>At5g08390.1 68418.m00988 transducin family protein / WD-40 repeat
           family protein similar to katanin p80 subunit
           [Strongylocentrotus purpuratus] GI:3005601; contains
           Pfam profile PF00400: WD domain, G-beta repeat
          Length = 871

 Score = 27.9 bits (59), Expect = 3.5
 Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 12/81 (14%)
 Frame = +1

Query: 124 GDEMTRIIWDLIKEKLILPF---------LDIELHVYDLGMENRDKTDDQVTIDCAEAIK 276
           G++    +WDL   KL+  F         LD   H + L   + DKT     ++  E I 
Sbjct: 255 GEDNVVKVWDLTAGKLLHEFKSHEGKIQSLDFHPHEFLLATGSADKTVKFWDLETFELIG 314

Query: 277 K---YNVGIKCATITPDEKRV 330
                  G++C T  PD K V
Sbjct: 315 SGGTETTGVRCLTFNPDGKSV 335


>At3g02250.1 68416.m00206 expressed protein contains Pfam PF03138:
           Plant protein family. The function of this family of
           plant proteins is unknown; previously annotated as
           'auxin-independent growth promoter -related'  based on
           similarity to axi 1 protein (GB:X80301) (GI:559920) from
           [Nicotiana tabacum], which, due to scienitific fraud was
           retracted. Retraction in: Schell J. EMBO J 1999 May
           17;18(10):2908. PMID:10400497.
          Length = 512

 Score = 27.9 bits (59), Expect = 3.5
 Identities = 11/18 (61%), Positives = 14/18 (77%)
 Frame = -1

Query: 407 LKTVPPRILRMVPLGLFH 354
           LK +PPR+ R V LGL+H
Sbjct: 176 LKELPPRLKRRVRLGLYH 193


>At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50)
           identical to DNA repair-recombination protein GI:7110148
           from [Arabidopsis thaliana]
          Length = 1316

 Score = 26.6 bits (56), Expect = 8.1
 Identities = 17/60 (28%), Positives = 30/60 (50%)
 Frame = +1

Query: 184 LDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYNVGIKCATITPDEKRVEEFKLKKMWKS 363
           +D E+H  ++ +++  K  DQV+I  AE    +    +     P+E      +LK+ WKS
Sbjct: 252 VDAEVHNKEMMLKDLRKLQDQVSIKTAERSTLFKEQQRQYAALPEENEDTIEELKE-WKS 310


>At1g12300.1 68414.m01422 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 637

 Score = 26.6 bits (56), Expect = 8.1
 Identities = 15/54 (27%), Positives = 28/54 (51%), Gaps = 3/54 (5%)
 Frame = +1

Query: 157 IKEKLILPFLDIELHVYDL---GMENRDKTDDQVTIDCAEAIKKYNVGIKCATI 309
           I EK+    +++++ +Y++   GM N  K DD   + C+  +K    G+K   I
Sbjct: 495 IFEKIEKSKMELDIGIYNIIIHGMCNASKVDDAWDLFCSLPLKGVKPGVKTYNI 548


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,336,527
Number of Sequences: 28952
Number of extensions: 209390
Number of successful extensions: 513
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 507
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 513
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 762235320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)