BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0279 (547 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.03c |med31|soh1, sep10|mediator complex subunit Med31|... 100 3e-22 SPBPB8B6.06c ||SPAPB8B6.06c, SPAPB8B6.06c|conserved fungal prote... 27 2.4 SPAC977.11 |||conserved fungal protein|Schizosaccharomyces pombe... 27 2.4 SPAC32A11.01 |mug8||conserved fungal protein|Schizosaccharomyces... 26 4.2 SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit |Schizosa... 26 4.2 SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 26 4.2 SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 25 9.6 SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pomb... 25 9.6 >SPCP31B10.03c |med31|soh1, sep10|mediator complex subunit Med31|Schizosaccharomyces pombe|chr 3|||Manual Length = 139 Score = 99.5 bits (237), Expect = 3e-22 Identities = 38/74 (51%), Positives = 58/74 (78%) Frame = +2 Query: 314 NRIRFQVELEFVQCLANPNYIHFLAQRGYLKEQTFVNYLKYLQYWREPEYARYLKYPMCL 493 ++ RF++ELEFVQ L+NP Y++FLAQ Y +++ F+ YL+Y++YWREPEY +++ YP CL Sbjct: 13 DKSRFEIELEFVQMLSNPWYLNFLAQHKYFEDEAFLQYLEYMEYWREPEYVKFIIYPTCL 72 Query: 494 HFLELLQHEAFRRE 535 H L LL++ FR + Sbjct: 73 HMLTLLKNPQFRND 86 >SPBPB8B6.06c ||SPAPB8B6.06c, SPAPB8B6.06c|conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 311 Score = 26.6 bits (56), Expect = 2.4 Identities = 12/32 (37%), Positives = 19/32 (59%) Frame = +1 Query: 121 SHIYIRIK*TVLCNLKICYYFGHRFVETYTTD 216 +HI +R+ +L L IC++ G F YTT+ Sbjct: 153 THIVVRV---LLLLLSICFFVGAAFYTAYTTN 181 >SPAC977.11 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 311 Score = 26.6 bits (56), Expect = 2.4 Identities = 12/32 (37%), Positives = 19/32 (59%) Frame = +1 Query: 121 SHIYIRIK*TVLCNLKICYYFGHRFVETYTTD 216 +HI +R+ +L L IC++ G F YTT+ Sbjct: 153 THIVVRV---LLLLLSICFFVGAAFYTAYTTN 181 >SPAC32A11.01 |mug8||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 25.8 bits (54), Expect = 4.2 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = +2 Query: 284 GKVMPETEEQNRIRFQVELEFVQCL 358 G V+P+ E +NRIR E + CL Sbjct: 81 GYVIPKHEIRNRIRLLSEASLISCL 105 >SPAC23A1.12c |||phenylalanine-tRNA ligase beta subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 25.8 bits (54), Expect = 4.2 Identities = 15/48 (31%), Positives = 26/48 (54%) Frame = -2 Query: 276 ILNPKYMQLEYLNYSKLNKLVSGVRLNKSMTKIITYFQVTQDSSFYPY 133 + NPK ++ + + S L ++ VR NK+ I F+V+ D +F Y Sbjct: 428 LANPKTLEFQVVRSSLLPGILKTVRENKNHALPIKIFEVS-DVAFCDY 474 >SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 1811 Score = 25.8 bits (54), Expect = 4.2 Identities = 18/54 (33%), Positives = 25/54 (46%) Frame = -1 Query: 232 KIKQVGQWCTSQQIYDQNNNIFSGYTRQFILSLYKYVIFKILSLLKCYTVLSKL 71 KIK + C Q I + NI SG+ F + Y I +L L +V+S L Sbjct: 1279 KIKDLVLRCIDQMIKARYQNIRSGWRTIFHILAYASKIENLLVLQCAISVVSSL 1332 >SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1233 Score = 24.6 bits (51), Expect = 9.6 Identities = 13/39 (33%), Positives = 19/39 (48%) Frame = +2 Query: 338 LEFVQCLANPNYIHFLAQRGYLKEQTFVNYLKYLQYWRE 454 LE+ +C+A + LA GY ++ L L Y RE Sbjct: 471 LEYNECVAAAQLLDHLAACGYFFFSKYIARLASLGYLRE 509 >SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pombe|chr 1|||Manual Length = 910 Score = 24.6 bits (51), Expect = 9.6 Identities = 8/18 (44%), Positives = 11/18 (61%) Frame = +3 Query: 333 WNLNLCSA*QIQITFTFW 386 WNL CSA + + +FW Sbjct: 390 WNLRKCSAAALDVLSSFW 407 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,328,134 Number of Sequences: 5004 Number of extensions: 47286 Number of successful extensions: 124 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 120 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 123 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 225926624 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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