Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0258
(639 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 180 9e-46
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 177 6e-45
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 157 5e-39
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 157 9e-39
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 101 7e-22
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 89 2e-18
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 87 9e-18
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 70 1e-12
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 6e-04
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.79
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.4
SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37) 29 3.2
SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9) 29 3.2
SB_34580| Best HMM Match : Gp-FAR-1 (HMM E-Value=0.88) 28 7.4
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 180 bits (438), Expect = 9e-46
Identities = 83/103 (80%), Positives = 94/103 (91%)
Frame = +3
Query: 330 DMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVIT 509
DM HWPF V+ +G +PKI+V +KGE K+FFPEE+SSMVLTKMKETAEAYLG V +AV+T
Sbjct: 86 DMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSMVLTKMKETAEAYLGAKVTDAVVT 145
Query: 510 VPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 638
VPAYF+DSQRQATKDAGTI+GLNVLRIINEPTAAAIAYGLDKK
Sbjct: 146 VPAYFHDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKK 188
Score = 161 bits (390), Expect = 6e-40
Identities = 74/82 (90%), Positives = 78/82 (95%)
Frame = +2
Query: 80 EAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 259
+ PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVAMN
Sbjct: 3 KTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVAMN 62
Query: 260 PNNTIFDAKRLIGRKFEDATVA 325
P NT+FDAKRLIGRKF+D VA
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVA 84
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 177 bits (431), Expect = 6e-45
Identities = 83/104 (79%), Positives = 94/104 (90%)
Frame = +3
Query: 324 QADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAV 503
Q+DMK WPF+++SD KPKI+V YKGE KTF EE+SSMVL KMKETAEAYLG+ V +AV
Sbjct: 84 QSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSMVLGKMKETAEAYLGQKVTSAV 143
Query: 504 ITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDK 635
ITVPAYFNDSQRQATKDAGTI+GLNVLR+INEPTAAA+AYGLDK
Sbjct: 144 ITVPAYFNDSQRQATKDAGTIAGLNVLRVINEPTAAALAYGLDK 187
Score = 159 bits (387), Expect = 1e-39
Identities = 73/91 (80%), Positives = 83/91 (91%)
Frame = +2
Query: 80 EAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 259
+APA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA+N
Sbjct: 3 KAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVALN 62
Query: 260 PNNTIFDAKRLIGRKFEDATVASRHEALAFR 352
P NTIFDAKRLIGR+++DATV S + F+
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFK 93
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 157 bits (382), Expect = 5e-39
Identities = 72/81 (88%), Positives = 77/81 (95%)
Frame = +2
Query: 80 EAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 259
+APA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVAMN
Sbjct: 3 KAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVAMN 62
Query: 260 PNNTIFDAKRLIGRKFEDATV 322
P NTIFDAKRLIGR+F+D V
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGV 83
Score = 127 bits (307), Expect = 7e-30
Identities = 62/71 (87%), Positives = 66/71 (92%)
Frame = +3
Query: 426 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPT 605
++SSMVL KMKETAEAYLG V +AV+TVPAYFNDSQRQATKDAG ISGLNVLRIINEPT
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAGVISGLNVLRIINEPT 270
Query: 606 AAAIAYGLDKK 638
AAAIAYGLDKK
Sbjct: 271 AAAIAYGLDKK 281
Score = 118 bits (284), Expect = 4e-27
Identities = 55/70 (78%), Positives = 62/70 (88%)
Frame = +3
Query: 351 EVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFND 530
EVV++ G+PK+KV YKGE KTFF EE+SSMVL KMKETAEAYLG V +AV+TVPAYFND
Sbjct: 118 EVVNEAGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFND 177
Query: 531 SQRQATKDAG 560
SQRQATKDAG
Sbjct: 178 SQRQATKDAG 187
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 157 bits (380), Expect = 9e-39
Identities = 75/105 (71%), Positives = 88/105 (83%)
Frame = +3
Query: 324 QADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAV 503
Q D+K +PF+VV KP ++V K E KTF EE+S+MVLTKMKETAEAYLGK V +AV
Sbjct: 782 QNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAMVLTKMKETAEAYLGKKVTHAV 841
Query: 504 ITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 638
+TVPAYFND+QRQATKDAG I+GL V+RIINEPTAAAIAYG+DKK
Sbjct: 842 VTVPAYFNDAQRQATKDAGAIAGLTVMRIINEPTAAAIAYGMDKK 886
Score = 131 bits (316), Expect = 5e-31
Identities = 62/98 (63%), Positives = 76/98 (77%), Gaps = 1/98 (1%)
Frame = +2
Query: 62 KIQNGREAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAA 238
K Q + +GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD A
Sbjct: 694 KEQREKVGTVIGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGA 753
Query: 239 KNQVAMNPNNTIFDAKRLIGRKFEDATVASRHEALAFR 352
KNQ+ NP NT+FDAKRLIGR ++D +V + + F+
Sbjct: 754 KNQLTTNPENTVFDAKRLIGRSWDDTSVQNDLKFFPFK 791
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 101 bits (241), Expect = 7e-22
Identities = 59/109 (54%), Positives = 74/109 (67%), Gaps = 24/109 (22%)
Frame = +3
Query: 381 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAG 560
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+FND+QRQATKDAG
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATKDAG 61
Query: 561 TISGLN------------------------VLRIINEPTAAAIAYGLDK 635
TI+GL+ VLRIINEPTAAAIAYGLDK
Sbjct: 62 TIAGLDVLSRTASERPPSRFRRRRPHGRSRVLRIINEPTAAAIAYGLDK 110
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 89.4 bits (212), Expect = 2e-18
Identities = 43/106 (40%), Positives = 68/106 (64%), Gaps = 1/106 (0%)
Frame = +3
Query: 324 QADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNA 500
Q ++ H P++VV I+V Y G+ + F PE+V +M+ T++K TAE L V +
Sbjct: 140 QKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAMLFTRLKTTAEIALKTKVTDC 199
Query: 501 VITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 638
VI+VP+Y+ D QR+ DA +GLN LR++N+ TA ++AYG+ K+
Sbjct: 200 VISVPSYYTDRQRRCMLDASATAGLNCLRLMNDTTAVSLAYGIYKQ 245
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +2
Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 247
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
Score = 28.3 bits (60), Expect = 5.6
Identities = 14/32 (43%), Positives = 16/32 (50%)
Frame = +2
Query: 257 NPNNTIFDAKRLIGRKFEDATVASRHEALAFR 352
N NTI KR IGRKF D V L ++
Sbjct: 118 NLKNTISQFKRFIGRKFSDPAVQKEIPHLPYK 149
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 87.4 bits (207), Expect = 9e-18
Identities = 47/102 (46%), Positives = 62/102 (60%), Gaps = 1/102 (0%)
Frame = +3
Query: 336 KHWPFEVVSDG-GKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITV 512
K + F V +D G V KG PEE+ V+ K++ AE L TV AV++V
Sbjct: 117 KLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGGYVIKKLRTMAERNLTTTVTKAVMSV 176
Query: 513 PAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKK 638
PA F+ QR AT A +++G+ VLR+INEPTAAA+AYGL KK
Sbjct: 177 PAEFDIKQRNATVKAASLAGITVLRVINEPTAAALAYGLHKK 218
Score = 86.6 bits (205), Expect = 2e-17
Identities = 38/74 (51%), Positives = 54/74 (72%), Gaps = 2/74 (2%)
Frame = +2
Query: 92 VGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPN 265
+GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q +NP
Sbjct: 34 IGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAEINPK 93
Query: 266 NTIFDAKRLIGRKF 307
NTI+DAKR IG+ F
Sbjct: 94 NTIYDAKRFIGKTF 107
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 70.1 bits (164), Expect = 1e-12
Identities = 34/85 (40%), Positives = 52/85 (61%)
Frame = +3
Query: 375 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKD 554
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP +FN ++R+A
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAERRALLR 126
Query: 555 AGTISGLNVLRIINEPTAAAIAYGL 629
A + GLNVL+I+N TA A+ YGL
Sbjct: 127 AAELVGLNVLQIMNSNTAVALNYGL 151
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 41.5 bits (93), Expect = 6e-04
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +2
Query: 143 GKVEIIANDQGNRTTPSYVAFTDTERLI 226
G IIAND G+R TP+ VAF+DTE+LI
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLI 110
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.79
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +2
Query: 152 EIIANDQGNRTTPSYVAFT-DTERL 223
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 2.4
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -2
Query: 260 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 162
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37)
Length = 420
Score = 29.1 bits (62), Expect = 3.2
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -2
Query: 278 RILCCWGSSPPGSWRH 231
R++CC G SP WRH
Sbjct: 395 RVVCCQGDSPSPRWRH 410
>SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9)
Length = 340
Score = 29.1 bits (62), Expect = 3.2
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -2
Query: 278 RILCCWGSSPPGSWRH 231
R++CC G SP WRH
Sbjct: 170 RVVCCQGDSPSPRWRH 185
>SB_34580| Best HMM Match : Gp-FAR-1 (HMM E-Value=0.88)
Length = 953
Score = 27.9 bits (59), Expect = 7.4
Identities = 15/59 (25%), Positives = 26/59 (44%), Gaps = 3/59 (5%)
Frame = +1
Query: 160 RQRPGQQDHSVLCCVHRHRASHRRCRQEPGG---DEPQQHNIRCQTSHRT*VRRCYCGK 327
RQ + +++ H R +R+ ++ GG +EP + R T T C+C K
Sbjct: 267 RQSAPSRSMAIINKTHGKRHGYRKLSRDCGGRRPNEPYERTARVHTILSTDTETCFCAK 325
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,687,683
Number of Sequences: 59808
Number of extensions: 490632
Number of successful extensions: 1371
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1247
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1369
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1608851125
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -