Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0256
(582 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g11980.1 68416.m01482 male sterility protein 2 (MS2) identica... 73 2e-13
At5g22500.1 68418.m02626 acyl CoA reductase, putative / male-ste... 60 1e-09
At3g44540.1 68416.m04787 acyl CoA reductase, putative similar to... 59 2e-09
At3g44550.1 68416.m04788 acyl CoA reductase, putative similar to... 58 4e-09
At3g44560.1 68416.m04789 acyl CoA reductase, putative similar to... 56 1e-08
At3g56700.1 68416.m06307 male sterility protein, putative simila... 55 4e-08
At3g60060.1 68416.m06707 hypothetical protein male sterility pro... 37 0.009
At4g33360.1 68417.m04743 terpene cyclase/mutase-related low simi... 32 0.24
At5g22420.1 68418.m02615 acyl CoA reductase, putative similar to... 31 0.56
At4g38850.1 68417.m05500 auxin-responsive protein / small auxin ... 28 5.2
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 27 9.1
>At3g11980.1 68416.m01482 male sterility protein 2 (MS2) identical
to male sterility protein 2 (MS2) SP:Q08891 (Arabidopsis
thaliana)
Length = 616
Score = 72.5 bits (170), Expect = 2e-13
Identities = 41/143 (28%), Positives = 71/143 (49%), Gaps = 6/143 (4%)
Frame = +2
Query: 170 RFYYRERPGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMR-KYLDQPIFSRIKY 346
+F GFL K +EK+ P + IY+LI+ K ER++ + LD +F+ +K
Sbjct: 132 KFLITGSTGFLAKVLIEKVLRMAPDVSKIYLLIKAKSKEAAIERLKNEVLDAELFNTLKE 191
Query: 347 EHPEYFK-----KIIPISGDITAPKLGLCDEERNILINEVSIVIHSAASVKLNDHLKFTL 511
H + K+IP++G+I +GL + + EV ++I+SAA+ N+ L
Sbjct: 192 THGASYMSFMLTKLIPVTGNICDSNIGLQADSAEEIAKEVDVIINSAANTTFNERYDVAL 251
Query: 512 NTNVGGTMKVLELVKEMKNLAMF 580
+ N G ++ K+ K L +F
Sbjct: 252 DINTRGPGNLMGFAKKCKKLKLF 274
>At5g22500.1 68418.m02626 acyl CoA reductase, putative /
male-sterility protein, putative similar to acyl CoA
reductase [Simmondsia chinensis] GI:5020215; contains
Pfam profile PF03015: Male sterility protein; identical
to cDNA male sterility 2-like protein GI:1491614
Length = 491
Score = 59.7 bits (138), Expect = 1e-09
Identities = 38/131 (29%), Positives = 66/131 (50%), Gaps = 8/131 (6%)
Frame = +2
Query: 191 PGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMR-KYLDQPIFSRIKYEHPE--- 358
PGFL K VEK+ P + IY+L+R + +R+R + ++ +F ++ E
Sbjct: 20 PGFLAKVLVEKILRLQPNVKKIYLLLRAPDEKSAMQRLRSEVMEIDLFKVLRNNLGEDNL 79
Query: 359 ---YFKKIIPISGDITAPKLGLCDEER-NILINEVSIVIHSAASVKLNDHLKFTLNTNVG 526
+KI+P+ GDI+ LGL D + + +E+ I+I+ AA+ ++ L N
Sbjct: 80 NALMREKIVPVPGDISIDNLGLKDTDLIQRMWSEIDIIINIAATTNFDERYDIGLGINTF 139
Query: 527 GTMKVLELVKE 559
G + VL K+
Sbjct: 140 GALNVLNFAKK 150
>At3g44540.1 68416.m04787 acyl CoA reductase, putative similar to
acyl CoA reductase [Simmondsia chinensis] GI:5020215;
contains Pfam profile PF03015: Male sterility protein
Length = 433
Score = 58.8 bits (136), Expect = 2e-09
Identities = 36/131 (27%), Positives = 69/131 (52%), Gaps = 8/131 (6%)
Frame = +2
Query: 191 PGFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMR-KYLDQPIFSRIKY----EHP 355
PGFL K +VEK+ P + +++L+R + +R + L++ +F +K E+
Sbjct: 20 PGFLAKVFVEKILRIQPKVKKLFLLLRAADNESAMQRFHSEVLEKDLFRVLKNALGDENL 79
Query: 356 EYF--KKIIPISGDITAPKLGLCDEE-RNILINEVSIVIHSAASVKLNDHLKFTLNTNVG 526
+ F +K++PI GDI+ LG+ + + NE+ I+++ AA+ ++ L+ N
Sbjct: 80 KAFITEKVVPIPGDISVDNLGVKGSDLLQHMWNEIDIIVNVAATTNFDERYDVGLSVNTF 139
Query: 527 GTMKVLELVKE 559
G + VL K+
Sbjct: 140 GPLNVLNFAKK 150
>At3g44550.1 68416.m04788 acyl CoA reductase, putative similar to
acyl CoA reductase [Simmondsia chinensis] GI:5020215;
contains Pfam profile PF03015: Male sterility protein
Length = 402
Score = 58.0 bits (134), Expect = 4e-09
Identities = 33/130 (25%), Positives = 64/130 (49%), Gaps = 8/130 (6%)
Frame = +2
Query: 194 GFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMR-KYLDQPIFSRIKYE------H 352
GFL K +VEK+ P + +Y+L+R +R+R + ++ +F ++ +
Sbjct: 21 GFLAKVFVEKILRVQPNVKKLYLLVRASDNEAATKRLRTEVFEKELFKVLRQNLGDEKLN 80
Query: 353 PEYFKKIIPISGDITAPKLGLCDEE-RNILINEVSIVIHSAASVKLNDHLKFTLNTNVGG 529
++K++ + GDI +LG+ D R + E+ IV++ AA+ ++ L N G
Sbjct: 81 TLLYEKVVSVPGDIATDQLGINDSHLRERMQKEIDIVVNVAATTNFDERYDVGLGINTFG 140
Query: 530 TMKVLELVKE 559
+ VL K+
Sbjct: 141 ALNVLNFAKK 150
>At3g44560.1 68416.m04789 acyl CoA reductase, putative similar to
acyl CoA reductase [Simmondsia chinensis] GI:5020215;
contains Pfam profile PF03015: Male sterility protein
Length = 449
Score = 56.4 bits (130), Expect = 1e-08
Identities = 33/130 (25%), Positives = 64/130 (49%), Gaps = 8/130 (6%)
Frame = +2
Query: 194 GFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMR-KYLDQPIFSRIKYE------H 352
GFL K +VEK+ P + +Y+++R +R+R + ++ +F ++ +
Sbjct: 21 GFLAKVFVEKILRVQPNVNKLYLVVRASDNEAATKRLRTEAFEKDLFKVLRDNLGDEKLN 80
Query: 353 PEYFKKIIPISGDITAPKLGLCDEE-RNILINEVSIVIHSAASVKLNDHLKFTLNTNVGG 529
+K++P++GDI LG+ D R + E+ IV++ AA+ ++ L N G
Sbjct: 81 TLLSEKVVPVAGDIAMDHLGMKDSNLRERMQKEIDIVVNVAATTNFDERYDIGLGINTFG 140
Query: 530 TMKVLELVKE 559
+ VL K+
Sbjct: 141 ALNVLNFAKK 150
>At3g56700.1 68416.m06307 male sterility protein, putative similar
to SP|Q08891 Male sterility protein 2 {Arabidopsis
thaliana}; contains Pfam profile PF03015: Male sterility
protein
Length = 527
Score = 54.8 bits (126), Expect = 4e-08
Identities = 32/118 (27%), Positives = 55/118 (46%), Gaps = 6/118 (5%)
Frame = +2
Query: 194 GFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMR-KYLDQPIFSRIKYEHPEYF-- 364
GFL K +EKL I I++L+R K + +R+ + + +F +K H +
Sbjct: 93 GFLAKVLIEKLLRESLEIGKIFLLMRSKDQESANKRLYDEIISSDLFKLLKQMHGSSYEA 152
Query: 365 ---KKIIPISGDITAPKLGLCDEERNILINEVSIVIHSAASVKLNDHLKFTLNTNVGG 529
+K+IP+ GDI LG+ E N++ E+ ++I +D L+ N G
Sbjct: 153 FMKRKLIPVIGDIEEDNLGIKSEIANMISEEIDVIISCGGRTTFDDRYDSALSVNALG 210
>At3g60060.1 68416.m06707 hypothetical protein male sterility
protein 2 - Brassica napus, PIR:T08096
Length = 154
Score = 37.1 bits (82), Expect = 0.009
Identities = 17/50 (34%), Positives = 26/50 (52%)
Frame = +2
Query: 350 HPEYFKKIIPISGDITAPKLGLCDEERNILINEVSIVIHSAASVKLNDHL 499
HP K++P+ DI LG+ E + E+ I+I SAA+ +D L
Sbjct: 49 HPSILSKLLPVVEDIAEDNLGVDSETSLKISEEIDIIISSAATTTFDDSL 98
>At4g33360.1 68417.m04743 terpene cyclase/mutase-related low
similarity to squalene-hopene cyclase from Zymomonas
mobilis [SP|P33990]
Length = 344
Score = 32.3 bits (70), Expect = 0.24
Identities = 20/41 (48%), Positives = 26/41 (63%), Gaps = 2/41 (4%)
Frame = +2
Query: 455 IVIHSAASVK--LNDHLKFTLNTNVGGTMKVLELVKEMKNL 571
IV H+AA V+ L D +F ++ NVGG VLE VKE K +
Sbjct: 78 IVFHAAALVEPWLPDPSRF-ISVNVGGLKNVLEAVKETKTV 117
>At5g22420.1 68418.m02615 acyl CoA reductase, putative similar to
acyl CoA reductase [Simmondsia chinensis] GI:5020215;
contains Pfam profile PF03015: Male sterility protein
Length = 409
Score = 31.1 bits (67), Expect = 0.56
Identities = 26/108 (24%), Positives = 44/108 (40%)
Frame = +2
Query: 194 GFLGKAYVEKLAYSCPGIVSIYILIRDKKGSNTEERMRKYLDQPIFSRIKYEHPEYFKKI 373
GFL K VE++ P + +Y+L+R + E+R L F I + + KK
Sbjct: 21 GFLAKVLVERILRLQPNVKRLYLLVRASDKKSAEQRYDVALGINTFGAINVLN--FAKKC 78
Query: 374 IPISGDITAPKLGLCDEERNILINEVSIVIHSAASVKLNDHLKFTLNT 517
+ + + +C E ++ + H A LN K +NT
Sbjct: 79 VKPKLLLHVSTVYVCGERPGHIVEK-----HFAMGESLNGKNKVDINT 121
>At4g38850.1 68417.m05500 auxin-responsive protein / small auxin up
RNA (SAUR-AC1) identical to GP:546362 small auxin up RNA
{Arabidopsis thaliana}; belongs to auxin-induced
(indole-3-acetic acid induced) protein family
Length = 89
Score = 27.9 bits (59), Expect = 5.2
Identities = 21/71 (29%), Positives = 37/71 (52%), Gaps = 2/71 (2%)
Frame = +2
Query: 197 FLG-KAYVEKLAYSCP-GIVSIYILIRDKKGSNTEERMRKYLDQPIFSRIKYEHPEYFKK 370
FLG K + + + S P G +++Y+ D+K + YL+QP+F ++ + E F
Sbjct: 7 FLGAKQIIRRESSSTPRGFMAVYVGENDQKKKRYVVPV-SYLNQPLFQQLLSKSEEEFGY 65
Query: 371 IIPISGDITAP 403
P+ G +T P
Sbjct: 66 DHPMGG-LTIP 75
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 27.1 bits (57), Expect = 9.1
Identities = 17/38 (44%), Positives = 20/38 (52%)
Frame = +2
Query: 380 ISGDITAPKLGLCDEERNILINEVSIVIHSAASVKLND 493
++GDI A GL E+R LI VS V A LND
Sbjct: 253 VAGDIPADMEGLVAEKRRELIETVSEVDDVLAEKFLND 290
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,292,243
Number of Sequences: 28952
Number of extensions: 254780
Number of successful extensions: 599
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 585
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 593
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1141585696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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