Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= P5PG0249
         (648 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    49   7e-07
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    41   1e-04
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    38   0.001
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos...    30   0.33 
SPBC902.04 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    29   0.58 
SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po...    28   1.0  
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma...    28   1.3  
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa...    26   4.1  
SPBC1347.03 |meu14||sporulation protein Meu14|Schizosaccharomyce...    26   5.4  
SPAC22H10.07 |scd2|ral3|scaffold protein Scd2|Schizosaccharomyce...    25   7.1  
SPBP19A11.01 |||glycine decarboxylase complex subunit H|Schizosa...    25   9.4  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 48.8 bits (111), Expect = 7e-07
 Identities = 26/71 (36%), Positives = 41/71 (57%), Gaps = 1/71 (1%)
 Frame = +3

Query: 243 KEIKQYEVTFNKFDEGRDGFLDLTEVKRMMERLGAPQTHLGLKAMISEVDEDGDNKISFR 422
           ++I ++   F+ FD  +DG +   E+  +M  LG   T   L+ MI+EVD DG+  I F 
Sbjct: 9   EQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFT 68

Query: 423 EFL-LIYRKAR 452
           EFL ++ RK +
Sbjct: 69  EFLTMMARKMK 79



 Score = 37.9 bits (84), Expect = 0.001
 Identities = 23/77 (29%), Positives = 40/77 (51%), Gaps = 6/77 (7%)
 Frame = +3

Query: 216 YTEFHEFSRKEIK----QYEV--TFNKFDEGRDGFLDLTEVKRMMERLGAPQTHLGLKAM 377
           +TEF     +++K    + EV   F  FD+  +G++ + E+  ++  LG   +   +  M
Sbjct: 67  FTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADM 126

Query: 378 ISEVDEDGDNKISFREF 428
           I E D DGD  I++ EF
Sbjct: 127 IREADTDGDGVINYEEF 143


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 41.1 bits (92), Expect = 1e-04
 Identities = 25/78 (32%), Positives = 40/78 (51%)
 Frame = +3

Query: 204 FVNVYTEFHEFSRKEIKQYEVTFNKFDEGRDGFLDLTEVKRMMERLGAPQTHLGLKAMIS 383
           FV V TE     R  +++ +  F  FD+   G + L  ++R+ + L        L+AMI 
Sbjct: 95  FVRVMTE-KIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIE 153

Query: 384 EVDEDGDNKISFREFLLI 437
           E D D D +I+ +EF+ I
Sbjct: 154 EFDLDQDGEINEQEFIAI 171


>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 29/81 (35%), Positives = 40/81 (49%), Gaps = 5/81 (6%)
 Frame = +3

Query: 201 KFVNVYTEFHEFSRKEIKQYEVTFNKFDEGRDGFL---DLTEVKRMM--ERLGAPQTHLG 365
           +F+N  + F     KE K  +  F  +D  RDG++   +L  V +MM    L   Q    
Sbjct: 77  EFINSLSVFSVHGNKEEK-LKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQI 135

Query: 366 LKAMISEVDEDGDNKISFREF 428
           +   I EVD+D D KISF EF
Sbjct: 136 VDKTIMEVDKDRDGKISFEEF 156



 Score = 34.3 bits (75), Expect = 0.015
 Identities = 20/66 (30%), Positives = 30/66 (45%)
 Frame = +3

Query: 234 FSRKEIKQYEVTFNKFDEGRDGFLDLTEVKRMMERLGAPQTHLGLKAMISEVDEDGDNKI 413
           FS +EI++    F K D  + G +D  E   +      P        + S VDEDG   +
Sbjct: 18  FSNEEIERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA----SRLFSVVDEDGGGDV 73

Query: 414 SFREFL 431
            F+EF+
Sbjct: 74  DFQEFI 79


>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
           Plc1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 899

 Score = 29.9 bits (64), Expect = 0.33
 Identities = 16/53 (30%), Positives = 26/53 (49%)
 Frame = +3

Query: 270 FNKFDEGRDGFLDLTEVKRMMERLGAPQTHLGLKAMISEVDEDGDNKISFREF 428
           + K ++ +   LDL +V RM + L    +   L+    + D D   K+SF EF
Sbjct: 303 WEKLEKEQSAQLDLGDVHRMCQMLHLNASMEFLEETFQKADADHSGKLSFEEF 355


>SPBC902.04 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 589

 Score = 29.1 bits (62), Expect = 0.58
 Identities = 21/99 (21%), Positives = 40/99 (40%)
 Frame = +3

Query: 159 NDKLEEGVEIKPKYKFVNVYTEFHEFSRKEIKQYEVTFNKFDEGRDGFLDLTEVKRMMER 338
           +  +E   ++ P     N   EFH+   +  +Q+E    + +  +    +L + KR + +
Sbjct: 336 SSNVESSEDVDPASLLQN--EEFHKLIEERQRQHEERLKRINANKKALEELNQKKRELAQ 393

Query: 339 LGAPQTHLGLKAMISEVDEDGDNKISFREFLLIYRKARA 455
               +  L L   I E D  G+ ++   E      KA A
Sbjct: 394 QQLKEQEL-LMQKIKETDRSGNKRLMLLETQHSLLKAEA 431


>SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 2812

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
 Frame = +3

Query: 180  VEIKPKY-KFVNVYTEFHEFSRKEIKQYEVTFNKFDEGRD 296
            V+   KY + + V T +   S  E K+Y + FN  DE  D
Sbjct: 1199 VDFSEKYTQLIPVITTYTHLSEVENKKYSLRFNSIDEALD 1238


>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 614

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 18/79 (22%), Positives = 35/79 (44%)
 Frame = +3

Query: 222 EFHEFSRKEIKQYEVTFNKFDEGRDGFLDLTEVKRMMERLGAPQTHLGLKAMISEVDEDG 401
           ++ E + +EI      FNK D    G+LD     +  E      ++  ++  I EV+ D 
Sbjct: 9   KYPELTNEEILTLTDQFNKLDVDGKGYLDQPTTIKAFED-SKKGSYDEVREAIREVNVDS 67

Query: 402 DNKISFREFLLIYRKARAG 458
             ++   +F+ I+   + G
Sbjct: 68  SGRVEPEDFVGIFNVLKKG 86


>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1072

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 11/31 (35%), Positives = 18/31 (58%)
 Frame = +1

Query: 556 RKLKSSLKVISFTTRLFKNKKRSVGKQRKEL 648
           R LKS    +S   ++  +K+R  GK+RK +
Sbjct: 550 RPLKSQKNTVSINNKIDTSKRRRKGKRRKRV 580


>SPBC1347.03 |meu14||sporulation protein Meu14|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 335

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 14/60 (23%), Positives = 26/60 (43%)
 Frame = +3

Query: 231 EFSRKEIKQYEVTFNKFDEGRDGFLDLTEVKRMMERLGAPQTHLGLKAMISEVDEDGDNK 410
           EF+RK     E + +  D G     ++ +VK +   L  P+       ++S + E+   K
Sbjct: 266 EFTRKSSSSVEFSDHSQDSGDPSQQNILQVKNVQAVLSIPEAESYKAQLLSSIAEEQKKK 325


>SPAC22H10.07 |scd2|ral3|scaffold protein Scd2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 536

 Score = 25.4 bits (53), Expect = 7.1
 Identities = 14/48 (29%), Positives = 22/48 (45%)
 Frame = +3

Query: 369 KAMISEVDEDGDNKISFREFLLIYRKARAGELEMDSGLDALARLTEIN 512
           K + SE D DG  +ISF +        R+   E+ SG   L  + + +
Sbjct: 86  KTVKSERDSDGSGQISFTDLTTNSSTTRSSISELHSGSQPLFGIVQFD 133


>SPBP19A11.01 |||glycine decarboxylase complex subunit
           H|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 169

 Score = 25.0 bits (52), Expect = 9.4
 Identities = 9/25 (36%), Positives = 15/25 (60%)
 Frame = -3

Query: 529 TPTCSTLISVNLAKASKPESISSSP 455
           +P   T+ S+N +    P+ +SSSP
Sbjct: 108 SPVSGTVTSINESLGDSPDKVSSSP 132


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,408,685
Number of Sequences: 5004
Number of extensions: 46937
Number of successful extensions: 159
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)