BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0242 (553 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 60 2e-10 SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 57 2e-09 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 48 7e-07 SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 42 8e-05 SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual 27 1.8 SPAC18B11.03c |||N-acetyltransferase |Schizosaccharomyces pombe|... 25 7.4 SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schiz... 25 9.8 SPAC2F3.08 |sut1||alpha-glucoside transporter |Schizosaccharomyc... 25 9.8 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 25 9.8 SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha su... 25 9.8 >SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pombe|chr 1|||Manual Length = 141 Score = 60.5 bits (140), Expect = 2e-10 Identities = 23/65 (35%), Positives = 48/65 (73%) Frame = +2 Query: 2 DPEDVIKNAFGCFDEENDGVIGEERLRELLTTMGDRFTDDDVDEMLREAPIRDGLFDYVE 181 DPE+ +K F FD++ G+IG LR +LT++G++ +++++DE+L+ P++DG+ +Y + Sbjct: 75 DPEEFVKG-FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVNYHD 133 Query: 182 FTRIL 196 F +++ Sbjct: 134 FVQMI 138 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 57.2 bits (132), Expect = 2e-09 Identities = 25/66 (37%), Positives = 45/66 (68%), Gaps = 1/66 (1%) Frame = +2 Query: 2 DPEDVIKNAFGCFDEENDGVIGEERLRELLTTMGDRFTDDDVDEMLREAPI-RDGLFDYV 178 D E+ ++ AF FD++ +G I E L +LT++G+R + ++V +M+REA DG+ +Y Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYE 141 Query: 179 EFTRIL 196 EF+R++ Sbjct: 142 EFSRVI 147 Score = 38.7 bits (86), Expect = 6e-04 Identities = 19/70 (27%), Positives = 34/70 (48%), Gaps = 1/70 (1%) Frame = +2 Query: 20 KNAFGCFDEENDGVIGEERLRELLTTMGDRFTDDDVDEMLREAPI-RDGLFDYVEFTRIL 196 + AF FD + DG I L ++ ++G T ++ +M+ E +G D+ EF ++ Sbjct: 15 REAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMM 74 Query: 197 KHGAKDKDEQ 226 KD D + Sbjct: 75 ARKMKDTDNE 84 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 48.4 bits (110), Expect = 7e-07 Identities = 23/62 (37%), Positives = 39/62 (62%), Gaps = 1/62 (1%) Frame = +2 Query: 8 EDVIKNAFGCFDEENDGVIGEERLRELLTTMGDRFTDDDVDEMLREA-PIRDGLFDYVEF 184 E+ IK AF FD++N G I + + + T+G++ +D++V M++EA P G FDY +F Sbjct: 78 EEYIK-AFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136 Query: 185 TR 190 + Sbjct: 137 VQ 138 >SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 41.5 bits (93), Expect = 8e-05 Identities = 23/72 (31%), Positives = 39/72 (54%) Frame = +2 Query: 5 PEDVIKNAFGCFDEENDGVIGEERLRELLTTMGDRFTDDDVDEMLREAPIRDGLFDYVEF 184 P + + AF FD+ G I +R+ L++MGDR +V+ +LR + G+F Y +F Sbjct: 115 PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILR-SYTSHGVFYYEKF 173 Query: 185 TRILKHGAKDKD 220 + G+KD + Sbjct: 174 VDAIA-GSKDSN 184 >SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual Length = 426 Score = 27.1 bits (57), Expect = 1.8 Identities = 12/44 (27%), Positives = 22/44 (50%), Gaps = 1/44 (2%) Frame = -1 Query: 148 CFPEHFIHIIVCKAIAHCGKQL-AESLLTNHTIVLFIEAPKCIF 20 CF F H I C A+ K + + +T +T++ + +P I+ Sbjct: 137 CFASIFFHAIACYALYLLTKSIFSNQKMTAYTVIFYCFSPSGIY 180 >SPAC18B11.03c |||N-acetyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 440 Score = 25.0 bits (52), Expect = 7.4 Identities = 10/34 (29%), Positives = 24/34 (70%) Frame = -1 Query: 304 NFSWLFHSAYLLHQLKSAARHGKLNLLLVLVFSA 203 +F+ +F+S ++L + A++GK+NL +++ +A Sbjct: 237 SFTSIFYSVFMLSIYYAIAKNGKVNLDMLIDVNA 270 >SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 24.6 bits (51), Expect = 9.8 Identities = 9/33 (27%), Positives = 22/33 (66%) Frame = +2 Query: 44 EENDGVIGEERLRELLTTMGDRFTDDDVDEMLR 142 E+N VI +++ + + ++G+R + D++E +R Sbjct: 111 EKNAYVINLKQIAKFVVSLGERVSPTDIEEGMR 143 >SPAC2F3.08 |sut1||alpha-glucoside transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 553 Score = 24.6 bits (51), Expect = 9.8 Identities = 8/27 (29%), Positives = 17/27 (62%) Frame = +3 Query: 111 LQTMMWMKCSGKHQSAMDSSIMWNSRV 191 + T+++++ G H+ D+SI W R+ Sbjct: 507 IATIVFIQSEGSHRDIADNSIAWVLRI 533 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 24.6 bits (51), Expect = 9.8 Identities = 15/68 (22%), Positives = 31/68 (45%) Frame = +2 Query: 17 IKNAFGCFDEENDGVIGEERLRELLTTMGDRFTDDDVDEMLREAPIRDGLFDYVEFTRIL 196 ++NA+ + ND + E++ +L+TT F ++ D + E P G+ E + Sbjct: 202 VENAYITNSQYNDTLDMEDQTSDLITT----FEHENEDHEVHEVPSIHGMEGTFETVNLT 257 Query: 197 KHGAKDKD 220 +K+ Sbjct: 258 SEAGNEKE 265 >SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha subunit Tfa1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 448 Score = 24.6 bits (51), Expect = 9.8 Identities = 9/20 (45%), Positives = 15/20 (75%) Frame = -1 Query: 292 LFHSAYLLHQLKSAARHGKL 233 L HSAY+LH++++ G+L Sbjct: 32 LRHSAYVLHKIRTILTFGRL 51 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,265,091 Number of Sequences: 5004 Number of extensions: 44660 Number of successful extensions: 134 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 133 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 229961028 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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