Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0224
(606 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09870.1 68414.m01111 histidine acid phosphatase family prote... 32 0.26
At3g10470.1 68416.m01255 zinc finger (C2H2 type) family protein ... 29 1.8
At2g21550.1 68415.m02565 bifunctional dihydrofolate reductase-th... 29 1.8
At4g25540.1 68417.m03682 DNA mismatch repair protein MSH3 (MSH3)... 29 2.4
At5g45560.1 68418.m05595 pleckstrin homology (PH) domain-contain... 29 3.2
At5g23580.1 68418.m02767 calcium-dependent protein kinase 9 (CDP... 29 3.2
At5g02940.1 68418.m00237 expressed protein 29 3.2
At2g45670.1 68415.m05678 calcineurin B subunit-related contains ... 28 4.2
At1g76550.1 68414.m08908 pyrophosphate--fructose-6-phosphate 1-p... 28 5.5
At1g52630.2 68414.m05942 expressed protein contains Pfam PF03138... 28 5.5
At1g52630.1 68414.m05943 expressed protein contains Pfam PF03138... 28 5.5
At5g04870.1 68418.m00510 calcium-dependent protein kinase isofor... 27 9.6
At3g28080.2 68416.m03506 nodulin MtN21 family protein similar to... 27 9.6
At3g28080.1 68416.m03505 nodulin MtN21 family protein similar to... 27 9.6
At2g47990.1 68415.m06006 transducin family protein / WD-40 repea... 27 9.6
At2g29560.1 68415.m03590 enolase, putative similar to enolase [S... 27 9.6
At2g07320.1 68415.m00839 SWIM zinc finger family protein contain... 27 9.6
>At1g09870.1 68414.m01111 histidine acid phosphatase family protein
contains Pfam profile PF00328: Histidine acid
phosphatase; similar to multiple inositol polyphosphate
phosphatase (GI:4105496)[Mus musculus]; EST gb|R64758
comes from this gene
Length = 487
Score = 32.3 bits (70), Expect = 0.26
Identities = 38/147 (25%), Positives = 58/147 (39%), Gaps = 8/147 (5%)
Frame = +2
Query: 182 TKTAYIFSNRGIPNA-KAHEVPG-CQPVAFWLLSRHGSHNPEANETAELQQLVDLKNNIV 355
T T Y S N + VP C P+ L++RHG+ +P EL+ L +V
Sbjct: 31 TVTRYSTSKDVTQNLIEGSNVPSECTPIHLNLVARHGTRSPTKKRLRELESLAGRFKELV 90
Query: 356 TNYKNGNFRNTNHRICTADF--NLLERWN--WKQNSSTAGDLTSDGYVTTQQLAQAWKQR 523
R+ R +D L +W W + G+L G QL ++R
Sbjct: 91 --------RDAEARKLPSDKIPGWLGQWKSPW-EGKVKGGELIRQGEDELYQLGIRVRER 141
Query: 524 FPGLLTPNTYN--YLFKFVEEPRSSNS 598
FP L + + Y + + PR+S S
Sbjct: 142 FPSLFEEDYHPDVYTIRATQIPRASAS 168
>At3g10470.1 68416.m01255 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 398
Score = 29.5 bits (63), Expect = 1.8
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = +2
Query: 254 PVAFWLLSRHGSHNPEANETAELQQLVDLKNNIVTNYKNGNFRNTNHRI 400
P+ F ++ SH P+A E E LV + ++ N N +N N+ +
Sbjct: 31 PIPFSIIPPMSSHEPDAEE--ESTSLVSKEKSLNDEINNNNIKNNNNTL 77
>At2g21550.1 68415.m02565 bifunctional dihydrofolate
reductase-thymidylate synthase, putative / DHFR-TS,
putative similar to THY-1 [SP| Q05762] and THY-2
[SP|Q05763] from Arabidopsis thaliana; contains Pfam
profiles PF00303 thymidylate synthase and PF00186
dihydrofolate reductase
Length = 492
Score = 29.5 bits (63), Expect = 1.8
Identities = 14/42 (33%), Positives = 24/42 (57%)
Frame = +1
Query: 460 RRSHQRRIRDDAAVGTGLEAAVPWTIDAEHLQLFVQIRRRTT 585
RRS+Q I +G G++ +PW + +E+ Q F + RT+
Sbjct: 21 RRSYQVVIAATRDMGLGMDMKLPWDLPSEY-QFFQDVTTRTS 61
>At4g25540.1 68417.m03682 DNA mismatch repair protein MSH3 (MSH3)
identical to SP|O65607 DNA mismatch repair protein MSH3
(AtMsh3) {Arabidopsis thaliana}
Length = 1081
Score = 29.1 bits (62), Expect = 2.4
Identities = 18/50 (36%), Positives = 22/50 (44%), Gaps = 1/50 (2%)
Frame = +2
Query: 287 SHNPEANETAE-LQQLVDLKNNIVTNYKNGNFRNTNHRICTADFNLLERW 433
S N E +A LQQL +KNN + F N NH + LL W
Sbjct: 415 SSNTEMTLSANTLQQLEVVKNNSDGSESGSLFHNMNHTLTVYGSRLLRHW 464
>At5g45560.1 68418.m05595 pleckstrin homology (PH) domain-containing
protein / lipid-binding START domain-containing protein
weak similarity to SP|P79245 Steroidogenic acute
regulatory protein, mitochondrial precursor (StAR) {Ovis
aries}; contains Pfam profiles PF01852: START domain,
PF00169: PH domain
Length = 719
Score = 28.7 bits (61), Expect = 3.2
Identities = 16/54 (29%), Positives = 26/54 (48%)
Frame = +2
Query: 374 NFRNTNHRICTADFNLLERWNWKQNSSTAGDLTSDGYVTTQQLAQAWKQRFPGL 535
N + +HRI A FN+ E WK+ D D V + Q +++ + PG+
Sbjct: 80 NKKEKHHRITMAAFNIQEALMWKEKIECVIDQHQDSLVPSGQQYVSFEYK-PGM 132
>At5g23580.1 68418.m02767 calcium-dependent protein kinase 9 (CDPK9)
identical to calcium-dependent protein kinase
[Arabidopsis thaliana] gi|836938|gb|AAA67653; contains
protein kinase domain, Pfam:PF00069; contains EF hand
domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 490
Score = 28.7 bits (61), Expect = 3.2
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +2
Query: 404 TADFNLLERWNWKQNSSTAGDLTSDGYVTTQQLAQAWKQ 520
T N LER + + D + GY+T ++L QAWK+
Sbjct: 388 TIHLNKLEREENLVAAFSFFDKDASGYITIEELQQAWKE 426
>At5g02940.1 68418.m00237 expressed protein
Length = 813
Score = 28.7 bits (61), Expect = 3.2
Identities = 15/41 (36%), Positives = 24/41 (58%)
Frame = -3
Query: 496 LRRHVSVAGEIAGRATVLFPVPTFQQVKVGRADSMISVPEV 374
LRR V++ G I G ++ PVP + V + DS+I + E+
Sbjct: 766 LRREVAI-GYIKGGKKIINPVPKTEPVSLEMEDSLIVISEL 805
>At2g45670.1 68415.m05678 calcineurin B subunit-related contains
Pfam PF00036: EF hand domain and Prosite PS00018:
EF-hand calcium-binding domain; contains Pfam profile
PF01553: Acyltransferase; weak similarity to Calcineurin
B subunit isoform 2 (Protein phosphatase 2B regulatory
subunit 2) (Protein phosphatase 3 regulatory subunit B
alpha isoform 2) (Swiss-Prot:Q63811) [Mus musculus]
Length = 539
Score = 28.3 bits (60), Expect = 4.2
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +2
Query: 464 DLTSDGYVTTQQLAQAWKQRFPGL 535
D DGY+T Q+L +A K P L
Sbjct: 470 DADGDGYITIQELGEALKNTIPNL 493
>At1g76550.1 68414.m08908 pyrophosphate--fructose-6-phosphate
1-phosphotransferase alpha subunit, putative /
pyrophosphate-dependent 6-phosphofructose-1-kinase,
putative strong similarity to SP|Q41140
Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
alpha subunit (EC 2.7.1.90) (PFP) (PPI-PFK) {Ricinus
communis}; contains Pfam profile PF00365:
Phosphofructokinase
Length = 617
Score = 27.9 bits (59), Expect = 5.5
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +3
Query: 399 SARPTLTCWNVGTGNRTVARPAISPAT 479
SA T+ W+ +G+ T+ RP I PA+
Sbjct: 485 SAMMTVKRWSQNSGSTTIGRPVIHPAS 511
>At1g52630.2 68414.m05942 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown;
Length = 351
Score = 27.9 bits (59), Expect = 5.5
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 491 TQQLAQAWKQRFPGLLTPNTYNYLFKFVEEPRSSNSF 601
T +LAQ W++R LTPN + + + P S+N +
Sbjct: 187 TGELAQTWRKRGKCPLTPNETVLMLQSLNIPTSTNIY 223
>At1g52630.1 68414.m05943 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown;
Length = 439
Score = 27.9 bits (59), Expect = 5.5
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 491 TQQLAQAWKQRFPGLLTPNTYNYLFKFVEEPRSSNSF 601
T +LAQ W++R LTPN + + + P S+N +
Sbjct: 275 TGELAQTWRKRGKCPLTPNETVLMLQSLNIPTSTNIY 311
>At5g04870.1 68418.m00510 calcium-dependent protein kinase isoform
AK1 (AK1) identical to calcium-dependent protein kinase,
isoform AK1 (CDPK) [Arabidopsis thaliana]
SWISS-PROT:Q06850; contains protein kinase domain,
Pfam:PF00069; contains EF hand domain (calcium-binding
EF-hand), Pfam:PF00036, INTERPRO:IPR002048
Length = 610
Score = 27.1 bits (57), Expect = 9.6
Identities = 23/83 (27%), Positives = 37/83 (44%)
Frame = +2
Query: 272 LSRHGSHNPEANETAELQQLVDLKNNIVTNYKNGNFRNTNHRICTADFNLLERWNWKQNS 451
L R G+ N + +E +L Q D+ N+ +YK T N +ER + +
Sbjct: 480 LKRVGA-NLKESEILDLMQAADVDNSGTIDYKE-------FIAATLHLNKIEREDHLFAA 531
Query: 452 STAGDLTSDGYVTTQQLAQAWKQ 520
T D GY+T +L QA ++
Sbjct: 532 FTYFDKDGSGYITPDELQQACEE 554
>At3g28080.2 68416.m03506 nodulin MtN21 family protein similar to
MtN21 GI:2598575 (root nodule development) from
[Medicago truncatula]
Length = 350
Score = 27.1 bits (57), Expect = 9.6
Identities = 13/50 (26%), Positives = 27/50 (54%)
Frame = -3
Query: 307 VSFRVMRAVTTEEPKGYGLASWYFMCLSVWDATIGEDVRGLRSE*QIWVL 158
VSF + + E P+ + ++ Y +C+S+ + IG V ++ IW++
Sbjct: 195 VSFILQTHIMREYPEAFTVSILYILCISIVTSMIGLVVE--KNNPSIWII 242
>At3g28080.1 68416.m03505 nodulin MtN21 family protein similar to
MtN21 GI:2598575 (root nodule development) from
[Medicago truncatula]
Length = 358
Score = 27.1 bits (57), Expect = 9.6
Identities = 13/50 (26%), Positives = 27/50 (54%)
Frame = -3
Query: 307 VSFRVMRAVTTEEPKGYGLASWYFMCLSVWDATIGEDVRGLRSE*QIWVL 158
VSF + + E P+ + ++ Y +C+S+ + IG V ++ IW++
Sbjct: 203 VSFILQTHIMREYPEAFTVSILYILCISIVTSMIGLVVE--KNNPSIWII 250
>At2g47990.1 68415.m06006 transducin family protein / WD-40 repeat
family protein similar to Vegetatible incompatibility
protein HET-E-1 (SP:Q00808) {Podospora anserina};
contains 5 WD-40 repeats (PF00400); similar to beta
transducin-like protein HET-E2C*4
(GP:17225206)[Podospora anserina]
Length = 530
Score = 27.1 bits (57), Expect = 9.6
Identities = 16/35 (45%), Positives = 17/35 (48%)
Frame = -2
Query: 116 LPSAG*RHTASFRGINVWD*LICGPKTARDATSHN 12
LPS G TA + VWD LI G K SHN
Sbjct: 231 LPSGGLIATAGGNSVKVWD-LIGGGKMVCSMESHN 264
>At2g29560.1 68415.m03590 enolase, putative similar to enolase
[Spinacia oleracea] gi|8919731|emb|CAB96173
Length = 475
Score = 27.1 bits (57), Expect = 9.6
Identities = 14/44 (31%), Positives = 27/44 (61%), Gaps = 1/44 (2%)
Frame = -3
Query: 496 LRRHVSVAGEIAGRATVLFPVPTFQQVKVGR-ADSMISVPEVSI 368
L +H+S +++GRA ++ PVP F + G+ A + ++ E+ I
Sbjct: 172 LCKHLS---DLSGRANMVLPVPAFTVLSGGKHASNTFAIQEIMI 212
>At2g07320.1 68415.m00839 SWIM zinc finger family protein contains
Pfam profile PF04434: SWIM zinc finger
Length = 534
Score = 27.1 bits (57), Expect = 9.6
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +2
Query: 248 CQPVAFWLLSRHGSHNPEANETAELQQLVDLKNNIV 355
C W+L+ G NPEAN EL Q ++K ++
Sbjct: 182 CFKTKTWVLTYKGIVNPEAN-ALELDQSEEVKERVI 216
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,101,939
Number of Sequences: 28952
Number of extensions: 261930
Number of successful extensions: 714
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 697
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 714
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1206913392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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