Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0213
(540 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2) id... 177 5e-45
At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1) (... 175 1e-44
At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1) 68 5e-12
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 44 9e-05
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 42 2e-04
At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2) ... 37 0.010
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 36 0.017
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 36 0.017
At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1) 36 0.017
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 36 0.023
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 36 0.023
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 30 0.86
At5g54080.2 68418.m06733 homogentisate 1,2-dioxygenase / homogen... 28 4.6
At5g54080.1 68418.m06732 homogentisate 1,2-dioxygenase / homogen... 28 4.6
At1g01130.1 68414.m00016 expressed protein ; expression supporte... 28 4.6
At5g57035.1 68418.m07119 protein kinase family protein contains ... 27 6.1
At5g52530.2 68418.m06518 dentin sialophosphoprotein-related cont... 27 6.1
At5g52530.1 68418.m06517 dentin sialophosphoprotein-related cont... 27 6.1
At3g49300.1 68416.m05388 proline-rich family protein contains pr... 27 6.1
At1g17270.1 68414.m02103 expressed protein 27 8.0
At1g09520.1 68414.m01067 expressed protein 27 8.0
>At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2)
identical to residues 14-204 of 20S proteasome beta
subunit PBC2 GB:AAC32069 [Arabidopsis thaliana]
Length = 204
Score = 177 bits (430), Expect = 5e-45
Identities = 80/146 (54%), Positives = 112/146 (76%)
Frame = +1
Query: 103 MSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDT 282
MSI YNG AVVAM G++C AIA+D+R G+Q QT++T+F ++ ++ L++GL GLATD
Sbjct: 1 MSIFEYNGSAVVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDHLFIGLSGLATDV 60
Query: 283 QTVFQRLKFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPY 462
QT++QRL FR LY+L+E R M+P+TF++++S +LYE+RFGP+ +PVIAGL DN+P+
Sbjct: 61 QTLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPFLCQPVIAGLGD-DNKPF 119
Query: 463 VCNMDLIGCPNEPEDFVVSGTCSEQL 540
+C MD IG +DFVVSGT SE L
Sbjct: 120 ICTMDSIGAKELAKDFVVSGTASESL 145
>At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1)
(PRCT) almost identical to GB:AAC32069 from [Arabidopsis
thaliana], EST gb|T76747 comes from this gene; identical
to cDNA proteasome subunit prct GI:2511567
Length = 204
Score = 175 bits (427), Expect = 1e-44
Identities = 79/146 (54%), Positives = 112/146 (76%)
Frame = +1
Query: 103 MSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDT 282
MSI YNG AVVAM G++C AIA+D+R G+Q QT++T+F ++ ++ +++GL GLATD
Sbjct: 1 MSIFEYNGSAVVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDRVFIGLSGLATDV 60
Query: 283 QTVFQRLKFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPY 462
QT++QRL FR LY+L+E R M+P+TF++++S +LYE+RFGPY +PVIAGL D++P+
Sbjct: 61 QTLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPYLCQPVIAGLGD-DDKPF 119
Query: 463 VCNMDLIGCPNEPEDFVVSGTCSEQL 540
+C MD IG +DFVVSGT SE L
Sbjct: 120 ICTMDSIGAKELAKDFVVSGTASESL 145
>At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1)
Length = 223
Score = 67.7 bits (158), Expect = 5e-12
Identities = 39/129 (30%), Positives = 62/129 (48%)
Frame = +1
Query: 100 NMSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATD 279
N S NGG VA+ G D IA D R +S ++ K+ ++ + G D
Sbjct: 7 NWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVLSSSGFQAD 66
Query: 280 TQTVFQRLKFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQP 459
+ + + LK R +Y+ + N+ M + +LSN LY +RF PY+ V+ GLD + +
Sbjct: 67 VKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLDE-EGKG 125
Query: 460 YVCNMDLIG 486
V D +G
Sbjct: 126 CVFTYDAVG 134
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 43.6 bits (98), Expect = 9e-05
Identities = 28/106 (26%), Positives = 48/106 (45%)
Frame = +1
Query: 124 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 303
G +A ++ V +A D R + S + K+ ++ P + + G A D Q + L
Sbjct: 57 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 116
Query: 304 KFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLD 441
+ L+EL R + S +L+N+LY R + +IAG D
Sbjct: 117 GIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWD 162
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 42.3 bits (95), Expect = 2e-04
Identities = 26/94 (27%), Positives = 43/94 (45%)
Frame = +1
Query: 160 VAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRLKFRMNLYELKEN 339
V +A D R + S + K+ ++ P + + G A D Q + L + L+EL
Sbjct: 69 VMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANK 128
Query: 340 RMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLD 441
R + S +L+N+LY R + +IAG D
Sbjct: 129 RRISVSGASKLLANMLYSYRGMGLSVGTMIAGWD 162
>At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2)
(PRS1) (PRC6) identical to SP|O24616 Proteasome subunit
alpha type 7-2 (EC 3.4.25.1) (20S proteasome alpha
subunit D2) {Arabidopsis thaliana}
Length = 250
Score = 36.7 bits (81), Expect = 0.010
Identities = 27/125 (21%), Positives = 53/125 (42%), Gaps = 4/125 (3%)
Frame = +1
Query: 124 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 303
G A V ++G D V +A +K+ + Q S + K+ + + + GL D + + +
Sbjct: 30 GNAAVGVRGTDTVVLAVEKKSTPKLQD-SRSARKIVSLDNHIALACAGLKADARVLINKA 88
Query: 304 KFRMNLYELKENRMMRPKTFSAMLSNLL--YERRFG--PYFIEPVIAGLDPYDNQPYVCN 471
+ + L + + + ++ L Y + G P+ + +I G DPY P +
Sbjct: 89 RIECQSHRLTLEDPVTVEYITRYIAGLQQKYTQSGGVRPFGLSTLIVGFDPYSRLPSLYQ 148
Query: 472 MDLIG 486
D G
Sbjct: 149 TDPSG 153
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 35.9 bits (79), Expect = 0.017
Identities = 26/106 (24%), Positives = 43/106 (40%)
Frame = +1
Query: 124 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 303
G +V + +D V + D R N K+ M P +Y G A DT+ V +
Sbjct: 39 GTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMV 98
Query: 304 KFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLD 441
++ L+ + R R T +L L+ + G V+ G+D
Sbjct: 99 SSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQ-GHVSAALVLGGVD 143
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 35.9 bits (79), Expect = 0.017
Identities = 26/106 (24%), Positives = 43/106 (40%)
Frame = +1
Query: 124 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 303
G +V + +D V + D R N K+ M P +Y G A DT+ V +
Sbjct: 39 GTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMV 98
Query: 304 KFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLD 441
++ L+ + R R T +L L+ + G V+ G+D
Sbjct: 99 SSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQ-GHVSAALVLGGVD 143
>At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1)
Length = 250
Score = 35.9 bits (79), Expect = 0.017
Identities = 27/125 (21%), Positives = 53/125 (42%), Gaps = 4/125 (3%)
Frame = +1
Query: 124 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 303
G A V ++G D V +A +K+ + Q S + K+ + + + GL D + + +
Sbjct: 30 GNAAVGVRGTDTVVLAVEKKSTPKLQD-SRSARKIVSLDNHIALACAGLKADARVLINKA 88
Query: 304 KFRMNLYELKENRMMRPKTFSAMLSNLL--YERRFG--PYFIEPVIAGLDPYDNQPYVCN 471
+ + L + + + ++ L Y + G P+ + +I G DPY P +
Sbjct: 89 RIECQSHRLTLEDPVTVEYITRYIAGLQQKYTQSGGVRPFGLSTLIVGFDPYTRIPALYQ 148
Query: 472 MDLIG 486
D G
Sbjct: 149 TDPSG 153
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 35.5 bits (78), Expect = 0.023
Identities = 26/106 (24%), Positives = 43/106 (40%)
Frame = +1
Query: 124 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 303
G +V + +D V + D R N K+ M P +Y G A DT+ V +
Sbjct: 39 GTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMV 98
Query: 304 KFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLD 441
++ L+ + R R T +L L+ + G V+ G+D
Sbjct: 99 SSQLRLHRYQTGRDSRVITALTLLKKHLFSYQ-GHVSAALVLGGVD 143
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 35.5 bits (78), Expect = 0.023
Identities = 26/106 (24%), Positives = 43/106 (40%)
Frame = +1
Query: 124 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 303
G +V + +D V + D R N K+ M P +Y G A DT+ V +
Sbjct: 39 GTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMV 98
Query: 304 KFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLD 441
++ L+ + R R T +L L+ + G V+ G+D
Sbjct: 99 SSQLRLHRYQTGRDSRVITALTLLKKHLFSYQ-GHVSAALVLGGVD 143
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 30.3 bits (65), Expect = 0.86
Identities = 26/139 (18%), Positives = 56/139 (40%)
Frame = +1
Query: 124 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 303
G ++ + V + D R + K+ Q+ +YV G A D+Q V +
Sbjct: 12 GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYV 71
Query: 304 KFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPYVCNMDLI 483
++ ++ + ++ + K + ++ L Y + ++ G D Y+ + + L
Sbjct: 72 RYFLHQHTIQHGQPATVKVSANLIRMLAYNNK-NMLQTGLIVGGWDKYEGGK-IYGIPLG 129
Query: 484 GCPNEPEDFVVSGTCSEQL 540
G E + F + G+ S L
Sbjct: 130 GTVVE-QPFAIGGSGSSYL 147
>At5g54080.2 68418.m06733 homogentisate 1,2-dioxygenase /
homogentisicase/homogentisate oxygenase / homogentisic
acid oxidase (HGO) identical to SP|Q9ZRA2 Homogentisate
1,2-dioxygenase (EC 1.13.11.5) (Homogentisicase)
(Homogentisate oxygenase) (Homogentisic acid oxidase)
{Arabidopsis thaliana}; contains Pfam profile PF04209:
homogentisate 1,2-dioxygenase
Length = 461
Score = 27.9 bits (59), Expect = 4.6
Identities = 14/33 (42%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = +1
Query: 373 LSNLLYERRFGPYFIEPVIAGLDPYD-NQPYVC 468
L L Y+ FG +F IAG P D N P +C
Sbjct: 8 LEELKYQSGFGNHFSSEAIAGALPLDQNSPLLC 40
>At5g54080.1 68418.m06732 homogentisate 1,2-dioxygenase /
homogentisicase/homogentisate oxygenase / homogentisic
acid oxidase (HGO) identical to SP|Q9ZRA2 Homogentisate
1,2-dioxygenase (EC 1.13.11.5) (Homogentisicase)
(Homogentisate oxygenase) (Homogentisic acid oxidase)
{Arabidopsis thaliana}; contains Pfam profile PF04209:
homogentisate 1,2-dioxygenase
Length = 461
Score = 27.9 bits (59), Expect = 4.6
Identities = 14/33 (42%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = +1
Query: 373 LSNLLYERRFGPYFIEPVIAGLDPYD-NQPYVC 468
L L Y+ FG +F IAG P D N P +C
Sbjct: 8 LEELKYQSGFGNHFSSEAIAGALPLDQNSPLLC 40
>At1g01130.1 68414.m00016 expressed protein ; expression supported
by MPSS
Length = 180
Score = 27.9 bits (59), Expect = 4.6
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +1
Query: 223 KVFQMGPTLYVGLPGLATDTQTVFQRLKFRMNLYE 327
KVF++ P+L+V D F ++ FR NLY+
Sbjct: 127 KVFEVAPSLHVVELRKTGDDTLEFHKVYFRFNLYQ 161
>At5g57035.1 68418.m07119 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain
Length = 786
Score = 27.5 bits (58), Expect = 6.1
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = -3
Query: 217 SWLILFELVYQTACP*RWPHSLGP 146
SW I F ++Y+TAC + H+ P
Sbjct: 514 SWFIRFRIIYETACGLAFLHNSKP 537
>At5g52530.2 68418.m06518 dentin sialophosphoprotein-related contains
weak similarity to dentin sialophosphoprotein precursor
(Dentin matrix protein-3) (DMP- 3) (Swiss-Prot:P97399)
[Mus musculus]
Length = 828
Score = 27.5 bits (58), Expect = 6.1
Identities = 28/115 (24%), Positives = 43/115 (37%)
Frame = +1
Query: 58 SNSKSRRCLSFYITNMSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQM 237
S S+SR + + N S GG + + V + KRF + + P+
Sbjct: 658 SRSRSRSPVLWNGRNRSPPPPPGGFRADERMTERVRLPFQKRFPADQEMGYMSPPRNRMS 717
Query: 238 GPTLYVGLPGLATDTQTVFQRLKFRMNLYELKENRMMRPKTFSAMLSNLLYERRF 402
P + G A + F+ KFR N M RP + + L + RRF
Sbjct: 718 SPRFFEGRNNGAGENYNSFRERKFRPGQRFYVGNSMRRPNSDNNNLRPFIPNRRF 772
>At5g52530.1 68418.m06517 dentin sialophosphoprotein-related contains
weak similarity to dentin sialophosphoprotein precursor
(Dentin matrix protein-3) (DMP- 3) (Swiss-Prot:P97399)
[Mus musculus]
Length = 828
Score = 27.5 bits (58), Expect = 6.1
Identities = 28/115 (24%), Positives = 43/115 (37%)
Frame = +1
Query: 58 SNSKSRRCLSFYITNMSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQM 237
S S+SR + + N S GG + + V + KRF + + P+
Sbjct: 658 SRSRSRSPVLWNGRNRSPPPPPGGFRADERMTERVRLPFQKRFPADQEMGYMSPPRNRMS 717
Query: 238 GPTLYVGLPGLATDTQTVFQRLKFRMNLYELKENRMMRPKTFSAMLSNLLYERRF 402
P + G A + F+ KFR N M RP + + L + RRF
Sbjct: 718 SPRFFEGRNNGAGENYNSFRERKFRPGQRFYVGNSMRRPNSDNNNLRPFIPNRRF 772
>At3g49300.1 68416.m05388 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 86
Score = 27.5 bits (58), Expect = 6.1
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +3
Query: 162 GHRYGQAVWYTSSNSINQLPKSIP 233
G R G V +T NS+N++ +SIP
Sbjct: 23 GFREGHGVTHTDQNSLNKVEESIP 46
>At1g17270.1 68414.m02103 expressed protein
Length = 564
Score = 27.1 bits (57), Expect = 8.0
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +1
Query: 244 TLYVGLPGLATDTQTVFQRLKFRMNLYELKENRM 345
TL+VGL L TDT+T F K +KE+ +
Sbjct: 78 TLFVGLLFLFTDTRTFFSSFKLDPMSSRVKESEL 111
>At1g09520.1 68414.m01067 expressed protein
Length = 260
Score = 27.1 bits (57), Expect = 8.0
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -3
Query: 397 VVHTISLTTSLRMF*AASFCFLLIHTSSF 311
V+HT+ L SLR F + C L H +SF
Sbjct: 36 VIHTVRLRASLRFF--CTHCLLRNHPASF 62
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,226,217
Number of Sequences: 28952
Number of extensions: 251666
Number of successful extensions: 564
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 554
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 562
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1003808112
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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