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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= P5PG0186
         (729 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_52788| Best HMM Match : No HMM Matches (HMM E-Value=.)             191   6e-49
SB_24934| Best HMM Match : No HMM Matches (HMM E-Value=.)             111   4e-25
SB_11283| Best HMM Match : No HMM Matches (HMM E-Value=.)             104   6e-23
SB_1643| Best HMM Match : No HMM Matches (HMM E-Value=.)               99   3e-21
SB_29968| Best HMM Match : No HMM Matches (HMM E-Value=.)              93   2e-19
SB_15646| Best HMM Match : Acyl-CoA_dh_M (HMM E-Value=8.3e-27)         69   4e-12
SB_7379| Best HMM Match : No HMM Matches (HMM E-Value=.)               52   5e-07
SB_10343| Best HMM Match : Acyl-CoA_dh_M (HMM E-Value=0.00044)         50   2e-06
SB_47347| Best HMM Match : Acyl-CoA_dh_1 (HMM E-Value=1e-17)           48   8e-06
SB_6242| Best HMM Match : No HMM Matches (HMM E-Value=.)               36   0.044
SB_55185| Best HMM Match : Acyl-CoA_dh_M (HMM E-Value=4.5e-16)         33   0.24 
SB_7831| Best HMM Match : RNA_pol_Rpb1_7 (HMM E-Value=0)               31   0.72 
SB_42487| Best HMM Match : Extensin_2 (HMM E-Value=0.084)              31   1.3  
SB_6033| Best HMM Match : RNA_pol_Rpb1_R (HMM E-Value=1.6e-37)         31   1.3  
SB_664| Best HMM Match : No HMM Matches (HMM E-Value=.)                30   1.7  
SB_50233| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.7  
SB_23753| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.7  
SB_23499| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   8.9  

>SB_52788| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 500

 Score =  191 bits (465), Expect = 6e-49
 Identities = 83/112 (74%), Positives = 101/112 (90%)
 Frame = +1

Query: 394 GGMDMGVFDGCLVAEELAYGCTGIMTAMEASGLGQTPIIIAGNKEQQKKYLGRLIDEPLV 573
           GG+ +GV DGC+V EEL+Y C+GI TA+EA+ L + P+I+AGN+EQ+KKYLGR+I EP++
Sbjct: 175 GGIGLGVLDGCVVTEELSYACSGIQTAIEANSLAEMPVILAGNEEQKKKYLGRMIKEPIM 234

Query: 574 AAYGVTEPGAGSDVAGIKTRAEKKGDEWILNGQKMWITNGGVANWYFVLART 729
           AAY VTEP AGSDVAGIKTRAEKKGDE+++NGQKMWITNGGVANWYFVLART
Sbjct: 235 AAYCVTEPTAGSDVAGIKTRAEKKGDEYVINGQKMWITNGGVANWYFVLART 286



 Score = 50.8 bits (116), Expect = 1e-06
 Identities = 20/38 (52%), Positives = 31/38 (81%)
 Frame = +1

Query: 211 TGFCFELTDEQKALQELARKFTKDEIIPVAGQYDKTGE 324
           +G  F ++DE +ALQ++ARKF ++EI+P A ++DKTGE
Sbjct: 32  SGISFAMSDELRALQDMARKFAREEIMPKAAEHDKTGE 69


>SB_24934| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 484

 Score =  111 bits (268), Expect = 4e-25
 Identities = 66/170 (38%), Positives = 94/170 (55%), Gaps = 3/170 (1%)
 Frame = +1

Query: 229 LTDEQKALQELARKFTKDEIIPVAGQYDKTGEYPW--PVVKKAWEVGLMNGHIPEHCGGM 402
           LT EQ  L+E+A  F ++E+ P A Q DK   +P      KK  ++G     +P   GG+
Sbjct: 41  LTSEQIHLREVAYNFCQNELAPYADQIDKENNFPKLREFWKKLGDLGFHGITVPVDDGGI 100

Query: 403 DMGVFDGCLVAEELAYGCTGIMTAMEA-SGLGQTPIIIAGNKEQQKKYLGRLIDEPLVAA 579
             G  +  +V EE++     I  +  A S L    I   GNKEQ++KYL +L+    + A
Sbjct: 101 GSGYMEHIIVLEEMSRVSGSIALSYGAHSNLCINQIARNGNKEQKRKYLPKLLSGEHIGA 160

Query: 580 YGVTEPGAGSDVAGIKTRAEKKGDEWILNGQKMWITNGGVANWYFVLART 729
             ++E  AGSDV  +K RA+K GD ++LNG KMWITNG  A+   V A+T
Sbjct: 161 LAMSEANAGSDVVSMKLRADKDGDHYVLNGHKMWITNGPDADVLIVYAKT 210


>SB_11283| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 631

 Score =  104 bits (250), Expect = 6e-23
 Identities = 62/169 (36%), Positives = 92/169 (54%), Gaps = 3/169 (1%)
 Frame = +1

Query: 232 TDEQKALQELARKFTKDEIIPVAGQYDKTGEYP-WPVVKKAWEVGLMNGHIPEHCGGMDM 408
           TD+   L+E   KF + EI P   +++K   +P   + KK  + G    + P   GGM +
Sbjct: 259 TDDHVQLRENLNKFIEKEINPFVDEWEKAKMFPAHELFKKFGKAGYFGVNKPVKYGGMGL 318

Query: 409 GVFDGCLVAEEL-AYGCTGIMTAMEASGLGQTPIIIA-GNKEQQKKYLGRLIDEPLVAAY 582
                  +AEEL +  C G+  A+       TP ++  G++E ++++L   I    V   
Sbjct: 319 DYSFSVAMAEELGSIKCGGVPMAIGVQADMATPALVRFGSEELKQQFLVPTIAGDFVTCL 378

Query: 583 GVTEPGAGSDVAGIKTRAEKKGDEWILNGQKMWITNGGVANWYFVLART 729
           GV+E GAGSDVA IKT A K GD++++NG KMW TNG  A+W  +LA T
Sbjct: 379 GVSETGAGSDVASIKTTAVKDGDDYVINGGKMWTTNGIQADWMCLLANT 427


>SB_1643| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 314

 Score = 99.1 bits (236), Expect = 3e-21
 Identities = 50/156 (32%), Positives = 80/156 (51%)
 Frame = +1

Query: 226 ELTDEQKALQELARKFTKDEIIPVAGQYDKTGEYPWPVVKKAWEVGLMNGHIPEHCGGMD 405
           +L +  + L++  R F  +E+ PVAG++DK   YP   VK   E+GL+   +PE  GG  
Sbjct: 40  KLPETHEMLRKTCRDFADNELTPVAGKFDKEHRYPQEKVKMLGELGLLGVEVPESLGGTG 99

Query: 406 MGVFDGCLVAEELAYGCTGIMTAMEASGLGQTPIIIAGNKEQQKKYLGRLIDEPLVAAYG 585
           +  F   +  EE++ GC  +   M  +          G   Q+++++   ++   V  + 
Sbjct: 100 LDAFAYAIAMEEISRGCASLGCIMSVNNY--------GTDAQKEEWIKPFLNGDRVGCFA 151

Query: 586 VTEPGAGSDVAGIKTRAEKKGDEWILNGQKMWITNG 693
           ++EP  GSD    KT A   GD ++LNG K WITNG
Sbjct: 152 LSEPDNGSDAGAAKTTAVLDGDHYVLNGTKAWITNG 187


>SB_29968| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 489

 Score = 93.5 bits (222), Expect = 2e-19
 Identities = 53/167 (31%), Positives = 89/167 (53%), Gaps = 1/167 (0%)
 Frame = +1

Query: 226 ELTDEQKALQELARKFTKDEIIPVAGQYDKTGEYPWPVVKKAWEVGLMNGHIPEHCGGMD 405
           +L++E++ +++   +F K++I P+  + D+  +    ++K  +E GLM   I    GG D
Sbjct: 41  QLSEEEEMMKQSVARFAKEKIQPLVSEMDQNSQMDPGIIKGLFEQGLMGIEIGSEYGGTD 100

Query: 406 MGVFDGCLVAEELAYGCTGIMTAMEASG-LGQTPIIIAGNKEQQKKYLGRLIDEPLVAAY 582
              F   LV EELA     +    +    L    I+  G KEQ+++YL +L    +V  +
Sbjct: 101 STFFAANLVIEELAKVDPSVSVMCDVQNTLINNLIMTYGTKEQKQEYLPQLAQH-MVGGF 159

Query: 583 GVTEPGAGSDVAGIKTRAEKKGDEWILNGQKMWITNGGVANWYFVLA 723
            ++E  +GSD   +KT A +K   ++LNG KMWITN   A  + V+A
Sbjct: 160 CLSEESSGSDAFALKTVALEKDGHFVLNGSKMWITNAEYAGVFLVMA 206


>SB_15646| Best HMM Match : Acyl-CoA_dh_M (HMM E-Value=8.3e-27)
          Length = 257

 Score = 68.9 bits (161), Expect = 4e-12
 Identities = 30/54 (55%), Positives = 41/54 (75%)
 Frame = +1

Query: 568 LVAAYGVTEPGAGSDVAGIKTRAEKKGDEWILNGQKMWITNGGVANWYFVLART 729
           +V   GV+E GAGSDVAG+KT A+K GD++++NG KMWITN   A++  +LA T
Sbjct: 1   MVGCIGVSEVGAGSDVAGLKTTAKKDGDDYVINGSKMWITNSPSADFICLLANT 54


>SB_7379| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 413

 Score = 52.0 bits (119), Expect = 5e-07
 Identities = 22/61 (36%), Positives = 40/61 (65%), Gaps = 1/61 (1%)
 Frame = +1

Query: 517 GNKEQQKKYLGRLIDEPLVAAYGVTEPG-AGSDVAGIKTRAEKKGDEWILNGQKMWITNG 693
           G+ EQ+KK+L  L++  + +A+ +TEPG A SD   ++ +  + GD +++NG+K W +  
Sbjct: 138 GSPEQKKKWLDPLLNGEMRSAFCMTEPGVASSDATNMECKITRHGDHYVINGRKWWSSGA 197

Query: 694 G 696
           G
Sbjct: 198 G 198


>SB_10343| Best HMM Match : Acyl-CoA_dh_M (HMM E-Value=0.00044)
          Length = 156

 Score = 50.0 bits (114), Expect = 2e-06
 Identities = 21/56 (37%), Positives = 38/56 (67%), Gaps = 1/56 (1%)
 Frame = +1

Query: 517 GNKEQQKKYLGRLIDEPLVAAYGVTEPG-AGSDVAGIKTRAEKKGDEWILNGQKMW 681
           G+ EQ+KK+L  L++  + +A+ +TEPG A SD   ++ +  + GD +++NG+K W
Sbjct: 98  GSPEQKKKWLDPLLNGDIRSAFCMTEPGVASSDATNMECKITRHGDHYVINGRKWW 153


>SB_47347| Best HMM Match : Acyl-CoA_dh_1 (HMM E-Value=1e-17)
          Length = 551

 Score = 48.0 bits (109), Expect = 8e-06
 Identities = 23/62 (37%), Positives = 39/62 (62%), Gaps = 1/62 (1%)
 Frame = +1

Query: 505 IIIAGNKEQQKKYLGRLIDEPLVAAYGVTEPG-AGSDVAGIKTRAEKKGDEWILNGQKMW 681
           +I  G+  Q++K+L  L+D  + + + +TEP  A SD A I++     GD ++LNG+K W
Sbjct: 318 LIRYGSDAQKEKWLEPLLDGRIRSCFAMTEPQVASSDAANIESSIRVDGDHYVLNGRKWW 377

Query: 682 IT 687
           I+
Sbjct: 378 IS 379


>SB_6242| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 686

 Score = 35.5 bits (78), Expect = 0.044
 Identities = 25/83 (30%), Positives = 42/83 (50%), Gaps = 8/83 (9%)
 Frame = +1

Query: 505 IIIAGNKEQQKKYLGRLIDEPLVAAYGVTEPGAGSDVAGIKTRA--EKKGDEWILN---- 666
           ++  G +    K L  +     V  +G+TE G G++   ++T A  +K+  E+++N    
Sbjct: 169 VLKLGTERHHSKLLKGIDSLQEVGCFGLTELGYGNNAVQMETTAVYDKETKEFVVNTPSP 228

Query: 667 -GQKMWITNGGV-ANWYFVLART 729
             QK WITNG   A+   V A+T
Sbjct: 229 LAQKYWITNGACHAHHIVVFAQT 251


>SB_55185| Best HMM Match : Acyl-CoA_dh_M (HMM E-Value=4.5e-16)
          Length = 647

 Score = 33.1 bits (72), Expect = 0.24
 Identities = 30/96 (31%), Positives = 44/96 (45%), Gaps = 9/96 (9%)
 Frame = +1

Query: 433 AEELAYGCTGIM-TAMEAS-GLGQTPIIIAGNKEQQKKYLGRLIDEPLVAAYGVTEPGAG 606
           A ELA    G M T M     L    ++  G +    K L  +     V  +G+TE G G
Sbjct: 122 AHELAAVVDGSMATKMTVQFNLFGGTVLKLGTERHHSKLLKGIDSLEDVGCFGLTELGYG 181

Query: 607 SDVAGIKTRA--EKKGDEWILN-----GQKMWITNG 693
           ++   ++T A  + +  E+I+N      QK WITNG
Sbjct: 182 NNAVQMETTATYDNETQEFIVNTPSTLAQKYWITNG 217


>SB_7831| Best HMM Match : RNA_pol_Rpb1_7 (HMM E-Value=0)
          Length = 1467

 Score = 31.5 bits (68), Expect = 0.72
 Identities = 34/126 (26%), Positives = 51/126 (40%), Gaps = 1/126 (0%)
 Frame = -2

Query: 668 PFRIHSSPFFSALVFIPATSDPAPGSVTP*AATNGSSISLPRYFFCCSLFPAIMMGVWPS 489
           P  + SSP  S     P+ S  +PG  +P + ++G S + P Y      +        P+
Sbjct: 417 PAFMPSSPSMSPAS--PSYSPTSPG-YSPSSPSSGYSPASPSYSPTSPSYSPTSPSYSPT 473

Query: 488 PLASIAVIIPVHPYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL- 312
             +         P + S +   PS +P S  P  S   P   PTS ++  T   YSP   
Sbjct: 474 SPSYSPTSPSYSPTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSP 531

Query: 311 SY*PAT 294
           SY P +
Sbjct: 532 SYSPTS 537



 Score = 29.1 bits (62), Expect = 3.9
 Identities = 37/120 (30%), Positives = 52/120 (43%), Gaps = 11/120 (9%)
 Frame = -2

Query: 620 PATSDP-APGSVTP*AATNGSSISLPRYFFCC-SLFPA------IMMGVWPS-PLASIAV 468
           P +  P +P   +P AA+ G S S P +     S+ PA         G  PS P +  + 
Sbjct: 392 PVSPGPTSPYIPSPAAASPGYSPSSPAFMPSSPSMSPASPSYSPTSPGYSPSSPSSGYSP 451

Query: 467 IIPVH-PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PAT 294
             P + P + S +   PS +P S  P  S   P   PTS ++  T   YSP   SY P +
Sbjct: 452 ASPSYSPTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTS 509



 Score = 28.3 bits (60), Expect = 6.7
 Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = -2

Query: 452 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PAT 294
           P + S +   PS +P S  P  S   P   PTS ++  T   YSP   SY P +
Sbjct: 500 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTS 551



 Score = 28.3 bits (60), Expect = 6.7
 Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = -2

Query: 452 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PAT 294
           P + S +   PS +P S  P  S   P   PTS ++  T   YSP   SY P +
Sbjct: 514 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTS 565



 Score = 28.3 bits (60), Expect = 6.7
 Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = -2

Query: 452 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PAT 294
           P + S +   PS +P S  P  S   P   PTS ++  T   YSP   SY P +
Sbjct: 528 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTS 579



 Score = 27.9 bits (59), Expect = 8.9
 Identities = 19/54 (35%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
 Frame = -2

Query: 452 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PAT 294
           P + S +   PS +P S  P  S   P   PTS ++  T   YSP   SY P++
Sbjct: 542 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPSYSPTSPSYSPSSPSYSPSS 593


>SB_42487| Best HMM Match : Extensin_2 (HMM E-Value=0.084)
          Length = 635

 Score = 30.7 bits (66), Expect = 1.3
 Identities = 18/54 (33%), Positives = 23/54 (42%), Gaps = 3/54 (5%)
 Frame = -3

Query: 427 DNHQTHPCPYHRNVLECVRSLGQLPKPSLQR---ATGTPPSYHIDPPQE*FHPS 275
           D    HP  +HR V    R + Q   PS+ R      TPPS  I   +   HP+
Sbjct: 450 DASPLHPAGFHRRVTPPSRGISQTRHPSIPRDLTDVCTPPSRGISQTRSPLHPA 503



 Score = 30.3 bits (65), Expect = 1.7
 Identities = 17/49 (34%), Positives = 22/49 (44%), Gaps = 3/49 (6%)
 Frame = -3

Query: 412 HPCPYHRNVLECVRSLGQLPKPSLQR---ATGTPPSYHIDPPQE*FHPS 275
           HP  +HR V    R + Q   PS+ R      TPPS  I   +   HP+
Sbjct: 199 HPAGFHRRVTPPSRGISQTRHPSIPRDFTDAFTPPSRGISQTRSPLHPA 247


>SB_6033| Best HMM Match : RNA_pol_Rpb1_R (HMM E-Value=1.6e-37)
          Length = 1052

 Score = 30.7 bits (66), Expect = 1.3
 Identities = 34/105 (32%), Positives = 45/105 (42%), Gaps = 2/105 (1%)
 Frame = -2

Query: 626 FIPATSDPAPGSVTP*AATNGSSISLPRYFFCC-SLFPAIMMGVWPSPLASIAVIIPVHP 450
           + P +   +P S +  +A+   S + P Y     S  PAI      SP  S A   P + 
Sbjct: 689 YSPTSPSYSPASPSYTSASPSYSPASPSYSPTSPSYSPAIPSNSPASPSYSPAS--PSYT 746

Query: 449 YASSSAT-RQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSP 318
            AS S T   PS TP S  P  +   P   PTS ++  T   YSP
Sbjct: 747 PASPSYTPASPSYTPAS--PSYTPTIPSYSPTSPSYSPTSPSYSP 789


>SB_664| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1046

 Score = 30.3 bits (65), Expect = 1.7
 Identities = 16/45 (35%), Positives = 20/45 (44%)
 Frame = +1

Query: 229 LTDEQKALQELARKFTKDEIIPVAGQYDKTGEYPWPVVKKAWEVG 363
           LT+    L  +  K    E    A  Y+ TG   W VV +AW VG
Sbjct: 591 LTESALNLASICSKVHHGEANLAADYYEMTGLPLWEVVNRAWNVG 635


>SB_50233| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 967

 Score = 28.3 bits (60), Expect = 6.7
 Identities = 18/59 (30%), Positives = 25/59 (42%)
 Frame = -2

Query: 452 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVLSY*PATGMISSF 276
           P  S S  R+PS  P S+P      C  + PTS   +  G G S    + P +  +  F
Sbjct: 651 PKTSGSLAREPSTAP-SLP------CDLVAPTSAKMYLAGQGGSGKTEWAPDSQQLEVF 702


>SB_23753| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 517

 Score = 28.3 bits (60), Expect = 6.7
 Identities = 17/55 (30%), Positives = 26/55 (47%)
 Frame = -2

Query: 482 ASIAVIIPVHPYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSP 318
           A+ + ++P  P +S S   QPS+TP+  PP   G       TS +   + H   P
Sbjct: 379 ATSSSMLPNTPSSSPSTGTQPSSTPIHEPP-TQGTTLVTMETSTSHLKSSHHSRP 432


>SB_23499| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 142

 Score = 27.9 bits (59), Expect = 8.9
 Identities = 18/59 (30%), Positives = 25/59 (42%)
 Frame = -2

Query: 527 SLFPAIMMGVWPSPLASIAVIIPVHPYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQ 351
           S+ P+I   + PS   SI   I      +S    QP++ P S P       P  +P SQ
Sbjct: 20  SIHPSIQPSIQPSIHPSIHPSIQPASQPASQPASQPASQPASQPASQPASQPASQPASQ 78


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,290,739
Number of Sequences: 59808
Number of extensions: 538636
Number of successful extensions: 2120
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1893
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2096
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1949964354
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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