Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0186
(729 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g45300.1 68416.m04891 isovaleryl-CoA-dehydrogenase (IVD) iden... 103 9e-23
At3g51840.1 68416.m05685 short-chain acyl-CoA oxidase identical ... 90 1e-18
At3g06810.1 68416.m00808 acyl-CoA dehydrogenase-related low simi... 46 2e-05
At4g16760.1 68417.m02531 acyl-CoA oxidase (ACX1) identical to ac... 40 0.002
At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest ... 32 0.45
At2g14050.1 68415.m01563 minichromosome maintenance family prote... 32 0.45
At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme... 31 0.78
At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger) fa... 30 1.4
At4g08400.1 68417.m01388 proline-rich extensin-like family prote... 30 1.8
At3g53860.1 68416.m05950 expressed protein several hypothetical ... 30 1.8
At4g23440.1 68417.m03379 expressed protein 29 3.2
At1g13960.2 68414.m01642 WRKY family transcription factor simila... 29 4.2
At1g13960.1 68414.m01641 WRKY family transcription factor simila... 29 4.2
At5g14240.1 68418.m01664 expressed protein 28 7.3
At4g27520.1 68417.m03952 plastocyanin-like domain-containing pro... 28 7.3
At1g50020.1 68414.m05613 expressed protein 27 9.6
>At3g45300.1 68416.m04891 isovaleryl-CoA-dehydrogenase (IVD)
identical to isovaleryl-CoA-dehydrogenase precursor
[Arabidopsis thaliana] GI:5596622
Length = 409
Score = 103 bits (248), Expect = 9e-23
Identities = 64/170 (37%), Positives = 88/170 (51%), Gaps = 5/170 (2%)
Frame = +1
Query: 235 DEQKALQELARKFTKDEIIPVAGQYDKTGEYPWPVVKKAWEV-GLMNGH---IPEHCGGM 402
D Q +E KF +D I P A + DKT +P V W++ G N H PE GG+
Sbjct: 33 DTQLQFKESVSKFAQDNIAPHAERIDKTNSFPKDV--NLWKLMGEFNLHGITAPEEYGGL 90
Query: 403 DMGVFDGCLVAEELAYGCTGIMTAMEA-SGLGQTPIIIAGNKEQQKKYLGRLIDEPLVAA 579
+G C+ EE++ + + A S L ++ G Q++KYL +LI V A
Sbjct: 91 GLGYLYHCIAMEEISRASGSVALSYGAHSNLCINQLVRNGTAAQKEKYLPKLISGEHVGA 150
Query: 580 YGVTEPGAGSDVAGIKTRAEKKGDEWILNGQKMWITNGGVANWYFVLART 729
++EP AGSDV G+K +AEK +ILNG KMW TNG A V A+T
Sbjct: 151 LAMSEPNAGSDVVGMKCKAEKVDGGYILNGNKMWCTNGPSAETLVVYAKT 200
>At3g51840.1 68416.m05685 short-chain acyl-CoA oxidase identical to
Short-chain acyl CoA oxidase [Arabidopsis thaliana]
GI:5478795; contains InterPro entry IPR006089: Acyl-CoA
dehydrogenase
Length = 436
Score = 90.2 bits (214), Expect = 1e-18
Identities = 51/167 (30%), Positives = 85/167 (50%), Gaps = 1/167 (0%)
Frame = +1
Query: 229 LTDEQKALQELARKFTKDEIIPVAGQYDKTGEYPWPVVKKAWEVGLMNGHIPEHCGGMDM 408
LT E++A+++ R+ + E+ P+ +Y + E+P+ + K +G+ G I + G +
Sbjct: 55 LTPEEQAIRKKVRECMEKEVAPIMTEYWEKAEFPFHITPKLGAMGVAGGSIKGY-GCPGL 113
Query: 409 GVFDGCLVAEELAYGCTGIMT-AMEASGLGQTPIIIAGNKEQQKKYLGRLIDEPLVAAYG 585
+ + E+A T + S LG I + G++ Q++KYL L VA +
Sbjct: 114 SITANAIATAEIARVDASCSTFILVHSSLGMLTIALCGSEAQKEKYLPSLAQLNTVACWA 173
Query: 586 VTEPGAGSDVAGIKTRAEKKGDEWILNGQKMWITNGGVANWYFVLAR 726
+TEP GSD +G+ T A K W +NGQK WI N A+ + AR
Sbjct: 174 LTEPDNGSDASGLGTTATKVEGGWKINGQKRWIGNSTFADLLIIFAR 220
>At3g06810.1 68416.m00808 acyl-CoA dehydrogenase-related low
similarity to acyl-CoA dehydrogenase [Acinetobacter sp.
NCIMB9871] GI:14587418; contains Pfam profiles PF01636:
Phosphotransferase enzyme family, PF00441: Acyl-CoA
dehydrogenase C-terminal domain, PF02770: Acyl-CoA
dehydrogenase middle domain
Length = 824
Score = 46.4 bits (105), Expect = 2e-05
Identities = 25/75 (33%), Positives = 43/75 (57%), Gaps = 2/75 (2%)
Frame = +1
Query: 481 ASGLGQTPIIIA-GNKEQQKKYLGRLIDEPLVAAYGVTEPG-AGSDVAGIKTRAEKKGDE 654
A G +I+ GNKEQ ++L L++ + + + +TEP A SD I+ ++GD
Sbjct: 521 APDTGNMEVILRYGNKEQISEWLIPLLEGRIRSGFAMTEPQVASSDATNIECSIRRQGDS 580
Query: 655 WILNGQKMWITNGGV 699
+++NG K W T+G +
Sbjct: 581 YVINGTKWW-TSGAM 594
>At4g16760.1 68417.m02531 acyl-CoA oxidase (ACX1) identical to
acyl-CoA oxidase [Arabidopsis thaliana] GI:3044214
Length = 675
Score = 39.9 bits (89), Expect = 0.002
Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +1
Query: 517 GNKEQQKKYLGRLIDEPLVAAYGVTEPGAGSDVAGIKTRA--EKKGDEWILN 666
G +EQQKK+L ++ Y TE G GS+V G++T A + K DE++++
Sbjct: 114 GTEEQQKKWLSLANKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIH 165
>At4g35800.1 68417.m05087 DNA-directed RNA polymerase II largest
subunit (RPB205) (RPII) (RPB1) nearly identical to
P|P18616 DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6) {Arabidopsis thaliana}
Length = 1840
Score = 31.9 bits (69), Expect = 0.45
Identities = 21/54 (38%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Frame = -2
Query: 452 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PAT 294
P + S + PS +P S P S P PTS A+ T GYSP SY P +
Sbjct: 1666 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTS 1717
Score = 29.5 bits (63), Expect = 2.4
Identities = 20/54 (37%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = -2
Query: 452 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSPVL-SY*PAT 294
P + S + PS +P S P S P PTS A+ T YSP SY P +
Sbjct: 1617 PTSPSYSPTSPSYSPTS--PSYSPTSPAYSPTSPAYSPTSPAYSPTSPSYSPTS 1668
Score = 28.7 bits (61), Expect = 4.2
Identities = 17/45 (37%), Positives = 21/45 (46%)
Frame = -2
Query: 452 PYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSP 318
P + S + PS +P S P S P PTS A+ T YSP
Sbjct: 1610 PTSPSYSPTSPSYSPTS--PSYSPTSPSYSPTSPAYSPTSPAYSP 1652
Score = 27.9 bits (59), Expect = 7.3
Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 2/62 (3%)
Frame = -2
Query: 461 PVHP-YASSSATRQPSNTPMS-IPPQCSGMCPFIRPTSQAFFTTGHGYSPVLSY*PATGM 288
P P Y+ +S P++ S P S P PTS ++ YSP ++Y P+
Sbjct: 1687 PTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYSPSIAYSPSNAR 1746
Query: 287 IS 282
+S
Sbjct: 1747 LS 1748
Score = 27.5 bits (58), Expect = 9.6
Identities = 18/47 (38%), Positives = 21/47 (44%), Gaps = 2/47 (4%)
Frame = -2
Query: 452 PYASSSATRQPSNTPMSIP--PQCSGMCPFIRPTSQAFFTTGHGYSP 318
PY A PS++P P P S P PTS + T GYSP
Sbjct: 1530 PYVGGMAF-SPSSSPGYSPSSPGYSPTSPGYSPTSPGYSPTSPGYSP 1575
>At2g14050.1 68415.m01563 minichromosome maintenance family protein
/ MCM family protein low similarity to SP|P49736 DNA
replication licensing factor MCM2 {Homo sapiens};
contains Pfam profile PF00493: MCM2/3/5 family
Length = 653
Score = 31.9 bits (69), Expect = 0.45
Identities = 27/103 (26%), Positives = 48/103 (46%), Gaps = 7/103 (6%)
Frame = +1
Query: 412 VFDGCLVAEELAYGCTGIMTAMEASGL---GQTPIIIAGN----KEQQKKYLGRLIDEPL 570
VF V +A G + ++ASG G++ +++ G+ K Q K+ +L + +
Sbjct: 320 VFGLFTVKLAVALTLIGGVQHVDASGTKVRGESHLLLIGDPGTGKSQFLKFAAKLSNRAV 379
Query: 571 VAAYGVTEPGAGSDVAGIKTRAEKKGDEWILNGQKMWITNGGV 699
+ G GS AG+ A K G EW+L + + +GG+
Sbjct: 380 ITT------GLGSTSAGLTVTAVKDGGEWMLEAGALVLADGGL 416
>At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme,
beta family protein similar to SP|P50867 Cysteine
synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase)
(O-acetylserine (Thiol)-lyase) [Aspergillus nidulans]
{Emericella nidulans}; contains Pfam profile PF00291:
Pyridoxal-phosphate dependent enzyme
Length = 421
Score = 31.1 bits (67), Expect = 0.78
Identities = 18/70 (25%), Positives = 29/70 (41%)
Frame = +1
Query: 418 DGCLVAEELAYGCTGIMTAMEASGLGQTPIIIAGNKEQQKKYLGRLIDEPLVAAYGVTEP 597
DG V A C G + + G G T + I + ++L + D + YG++
Sbjct: 354 DGLFVGSSSAMNCVGAVRVAQTLGPGHTIVTILCDSGM--RHLSKFHDPKYLNLYGLSPT 411
Query: 598 GAGSDVAGIK 627
G + GIK
Sbjct: 412 AIGLEFLGIK 421
>At5g42940.1 68418.m05235 zinc finger (C3HC4-type RING finger)
family protein similar to Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 691
Score = 30.3 bits (65), Expect = 1.4
Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = -2
Query: 464 IPVHPYASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFF-TTGHGYSPV 315
+ + P S +R P++TP+SIPP M P R T + T + +PV
Sbjct: 309 LDLRPGQSFVVSRNPNSTPVSIPPGSRTMLPPFRWTGSSLVGGTSNSTAPV 359
>At4g08400.1 68417.m01388 proline-rich extensin-like family protein
contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 513
Score = 29.9 bits (64), Expect = 1.8
Identities = 16/56 (28%), Positives = 27/56 (48%)
Frame = -3
Query: 442 APQPQDNHQTHPCPYHRNVLECVRSLGQLPKPSLQRATGTPPSYHIDPPQE*FHPS 275
+P P+ N+++ P PY+R+ S P P + + PP + PP + PS
Sbjct: 248 SPSPKVNYKSPPPPYYRSPPPPYYS----PSPKVDYKSPPPPYVYSSPPPPYYSPS 299
>At3g53860.1 68416.m05950 expressed protein several hypothetical
proteins - Arabidopsis thaliana
Length = 281
Score = 29.9 bits (64), Expect = 1.8
Identities = 13/27 (48%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
Frame = +3
Query: 597 WCWIRCSRYKDESGE--ERRRMDPERS 671
WC S++KDE + +RRR+DPE S
Sbjct: 113 WCKYGVSQFKDEQNQSNKRRRLDPEGS 139
>At4g23440.1 68417.m03379 expressed protein
Length = 964
Score = 29.1 bits (62), Expect = 3.2
Identities = 9/26 (34%), Positives = 17/26 (65%)
Frame = +1
Query: 622 IKTRAEKKGDEWILNGQKMWITNGGV 699
++ EK+GD W +G ++W+ GG+
Sbjct: 231 VRDIVEKRGDLWEKHGGELWVLYGGI 256
>At1g13960.2 68414.m01642 WRKY family transcription factor similar
to WKRY DNA-binding protein GB:AAD17441
Length = 487
Score = 28.7 bits (61), Expect = 4.2
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = -2
Query: 446 ASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSP 318
++S +T PS +S+PP RP S+ FF G G+SP
Sbjct: 9 STSKSTGAPSRPTLSLPP---------RPFSEMFFNGGVGFSP 42
>At1g13960.1 68414.m01641 WRKY family transcription factor similar
to WKRY DNA-binding protein GB:AAD17441
Length = 514
Score = 28.7 bits (61), Expect = 4.2
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = -2
Query: 446 ASSSATRQPSNTPMSIPPQCSGMCPFIRPTSQAFFTTGHGYSP 318
++S +T PS +S+PP RP S+ FF G G+SP
Sbjct: 9 STSKSTGAPSRPTLSLPP---------RPFSEMFFNGGVGFSP 42
>At5g14240.1 68418.m01664 expressed protein
Length = 256
Score = 27.9 bits (59), Expect = 7.3
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = +1
Query: 523 KEQQKKYLGRLIDEPLVAAYGVTEPGAGSDVAGIKTRAEKKGDEWIL 663
+E +KK L L + V YG P +GSD T+A ++W++
Sbjct: 80 EEYRKKRLSELREAAKVKRYGTVTPISGSDFVREVTQA--SAEDWVV 124
>At4g27520.1 68417.m03952 plastocyanin-like domain-containing
protein similar to PIR|JC7196 phytocyanin-related
protein Pn14 {Ipomoea nil}; contains Pfam profile
PF02298: Plastocyanin-like domain
Length = 349
Score = 27.9 bits (59), Expect = 7.3
Identities = 30/87 (34%), Positives = 40/87 (45%), Gaps = 1/87 (1%)
Frame = -2
Query: 650 SPFFSALVFIPATSDPAPGSVTP*AATNGSSISLPRYFFCCSLFPAIMMGVWPSPLASIA 471
SP S+ V PATS P GS+ P + + S + P PA P +S
Sbjct: 186 SPKSSSAVS-PATSPP--GSMAPKSGSPVSPTTSP---------PAPPKSTSPVSPSSAP 233
Query: 470 VIIPVHPYA-SSSATRQPSNTPMSIPP 393
+ P P A SS+T PS+ PM+ PP
Sbjct: 234 MTSPPAPMAPKSSSTIPPSSAPMTSPP 260
>At1g50020.1 68414.m05613 expressed protein
Length = 209
Score = 27.5 bits (58), Expect = 9.6
Identities = 12/53 (22%), Positives = 23/53 (43%)
Frame = -2
Query: 551 LPRYFFCCSLFPAIMMGVWPSPLASIAVIIPVHPYASSSATRQPSNTPMSIPP 393
+PR ++ +G WP + ++ ++ Y + S TP+S PP
Sbjct: 136 IPRARILLTIGGTFFLGFWPLIVLTLGAFSALYLYFGADFIHDGSRTPVSPPP 188
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,946,436
Number of Sequences: 28952
Number of extensions: 390462
Number of successful extensions: 1312
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1077
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1267
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -