Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0179
(694 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g47510.1 68415.m05930 fumarate hydratase, putative / fumarase... 234 4e-62
At5g50950.2 68418.m06319 fumarate hydratase, putative / fumarase... 231 3e-61
At5g50950.1 68418.m06318 fumarate hydratase, putative / fumarase... 231 3e-61
At1g13020.1 68414.m01510 eukaryotic translation initiation facto... 29 2.2
At5g13700.1 68418.m01595 polyamine oxidase, putative similar to ... 28 6.8
At4g11610.1 68417.m01859 C2 domain-containing protein contains I... 27 8.9
>At2g47510.1 68415.m05930 fumarate hydratase, putative / fumarase,
putative similar to SP|P55250 Fumarate hydratase,
mitochondrial precursor (EC 4.2.1.2) (Fumarase)
{Rhizopus oryzae}; contains Pfam profile PF00206: Lyase
Length = 492
Score = 234 bits (573), Expect = 4e-62
Identities = 114/179 (63%), Positives = 135/179 (75%)
Frame = +2
Query: 158 NISTTSVTARKEKDTFGELDVPDDKLYGAQTVRSVMNFPIGGIEERMPYPVIVAFGILKK 337
++ + S + R+E+DTFG + VP DKL+GAQT RS+ NF IGG ERMP P++ AFG+LKK
Sbjct: 24 SLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGERERMPEPIVRAFGVLKK 83
Query: 338 AAAKVNIEYGLEKKIADAIMQACDDVISGKLYREGHFPLVIWQTGSGTQSNMNTNEVIAN 517
AAKVN+EYGL+ I AIMQA +V GKL HFPLV+WQTGSGTQSNMN NEVIAN
Sbjct: 84 CAAKVNMEYGLDPTIGKAIMQAAQEVAEGKL--NDHFPLVVWQTGSGTQSNMNANEVIAN 141
Query: 518 RAIQILGGKLGSKDPVHPNDHVNKSQSSNDTYPTXMHIAVAMELRDRLMPGLVXLRDTL 694
RA +ILG K G K VHPNDHVN+SQSSNDT+PT MHIA A E+ RL+P L L TL
Sbjct: 142 RAAEILGRKRGEK-CVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRLIPSLKTLHSTL 199
>At5g50950.2 68418.m06319 fumarate hydratase, putative / fumarase,
putative similar to SP|P55250 Fumarate hydratase,
mitochondrial precursor (EC 4.2.1.2) (Fumarase)
{Rhizopus oryzae}; contains Pfam profile PF00206: Lyase
Length = 499
Score = 231 bits (565), Expect = 3e-61
Identities = 112/174 (64%), Positives = 132/174 (75%)
Frame = +2
Query: 173 SVTARKEKDTFGELDVPDDKLYGAQTVRSVMNFPIGGIEERMPYPVIVAFGILKKAAAKV 352
S R+E+DTFG + VP DKL+GAQT RS+ NF IGG ERMP P++ AFG+LKK AAKV
Sbjct: 36 STPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCAAKV 95
Query: 353 NIEYGLEKKIADAIMQACDDVISGKLYREGHFPLVIWQTGSGTQSNMNTNEVIANRAIQI 532
N+EYGL+ I +AIM+A +V GKL HFPLV+WQTGSGTQSNMN NEVIANRA +I
Sbjct: 96 NMEYGLDPMIGEAIMEAAQEVAEGKL--NDHFPLVVWQTGSGTQSNMNANEVIANRAAEI 153
Query: 533 LGGKLGSKDPVHPNDHVNKSQSSNDTYPTXMHIAVAMELRDRLMPGLVXLRDTL 694
LG K G K VHPNDHVN+SQSSNDT+PT MHIA A E+ RL+P L L +L
Sbjct: 154 LGHKRGEK-IVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKNLHSSL 206
>At5g50950.1 68418.m06318 fumarate hydratase, putative / fumarase,
putative similar to SP|P55250 Fumarate hydratase,
mitochondrial precursor (EC 4.2.1.2) (Fumarase)
{Rhizopus oryzae}; contains Pfam profile PF00206: Lyase
Length = 510
Score = 231 bits (565), Expect = 3e-61
Identities = 112/174 (64%), Positives = 132/174 (75%)
Frame = +2
Query: 173 SVTARKEKDTFGELDVPDDKLYGAQTVRSVMNFPIGGIEERMPYPVIVAFGILKKAAAKV 352
S R+E+DTFG + VP DKL+GAQT RS+ NF IGG ERMP P++ AFG+LKK AAKV
Sbjct: 36 STPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCAAKV 95
Query: 353 NIEYGLEKKIADAIMQACDDVISGKLYREGHFPLVIWQTGSGTQSNMNTNEVIANRAIQI 532
N+EYGL+ I +AIM+A +V GKL HFPLV+WQTGSGTQSNMN NEVIANRA +I
Sbjct: 96 NMEYGLDPMIGEAIMEAAQEVAEGKL--NDHFPLVVWQTGSGTQSNMNANEVIANRAAEI 153
Query: 533 LGGKLGSKDPVHPNDHVNKSQSSNDTYPTXMHIAVAMELRDRLMPGLVXLRDTL 694
LG K G K VHPNDHVN+SQSSNDT+PT MHIA A E+ RL+P L L +L
Sbjct: 154 LGHKRGEK-IVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKNLHSSL 206
>At1g13020.1 68414.m01510 eukaryotic translation initiation factor,
putative (EIF4B5) eukaryotic initiation factor 4B
(GI:6739522) {Arabidopsis thaliana}; EST gb|T22808 comes
from this gene
Length = 549
Score = 29.5 bits (63), Expect = 2.2
Identities = 18/52 (34%), Positives = 23/52 (44%)
Frame = +1
Query: 436 RGSLPPRHLADWLRYSVQHEHERGDCEPRHTNTGRETGQQGPGASQRPREQV 591
RG LP R + E + D E T T +T + P + RPREQV
Sbjct: 253 RGVLPSGGGVQEERRRLVFEPRKADTEVSETPTAVKTSKPSPFGAARPREQV 304
>At5g13700.1 68418.m01595 polyamine oxidase, putative similar to
SP|O64411 Polyamine oxidase precursor (EC 1.5.3.11) from
Zea mays
Length = 472
Score = 27.9 bits (59), Expect = 6.8
Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 6/67 (8%)
Frame = +2
Query: 308 VIVAFGILKKAAAKVNIEYGLEKKIADAIMQACDDVISGKLYRE--GHFPLVI---WQTG 472
+I+ GI +AAKV +E G+E D ++ D I G+++++ G P+ + W G
Sbjct: 7 IIIGAGISGISAAKVLVENGVE----DVLILEATDRIGGRIHKQNFGDVPVELGAGWIAG 62
Query: 473 -SGTQSN 490
G +SN
Sbjct: 63 VGGKESN 69
>At4g11610.1 68417.m01859 C2 domain-containing protein contains
INTERPRO:IPR000008 C2 domain
Length = 1011
Score = 27.5 bits (58), Expect = 8.9
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -2
Query: 399 CMIASAIFFSSPYSILTLAAAFFSI 325
C IA+ +FF +P I+ A FF++
Sbjct: 959 CFIAAIVFFITPIQIVVALAGFFTM 983
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,490,081
Number of Sequences: 28952
Number of extensions: 306532
Number of successful extensions: 874
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 849
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 868
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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