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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= P5PG0175
         (598 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar...   186   3e-48
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc...    87   2e-18
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar...    51   1e-07
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy...    46   4e-06
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch...    44   1e-05
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy...    35   0.008
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar...    34   0.018
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce...    32   0.073
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom...    29   0.68 
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces...    25   6.3  

>SPAC23D3.07 |pup1||20S proteasome component beta
           2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 267

 Score =  186 bits (452), Expect = 3e-48
 Identities = 83/140 (59%), Positives = 105/140 (75%)
 Frame = +1

Query: 175 GFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQK 354
           GF FE +QRN  L +KGFP PKAT TGTTIVG+I  D ++LGADTRAT   +++DKNC+K
Sbjct: 9   GFDFEYYQRNLLLQEKGFPTPKATSTGTTIVGVIAKDCIVLGADTRATAGPIIADKNCKK 68

Query: 355 IHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRYQGH 534
           +H ++ N++C GAGTAADTE  T  ++S +EL  L+T R   V TA T+LK+ LFRYQGH
Sbjct: 69  LHLISPNIWCAGAGTAADTEFVTSMISSNIELHSLYTNRKPRVVTALTMLKQHLFRYQGH 128

Query: 535 IGAALVLGGVDRTGPHIYCI 594
           IGA LVLGG D  GPH++ I
Sbjct: 129 IGAYLVLGGYDCKGPHLFTI 148


>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 87.0 bits (206), Expect = 2e-18
 Identities = 42/117 (35%), Positives = 66/117 (56%), Gaps = 1/117 (0%)
 Frame = +1

Query: 247 KTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQ 426
           + GTTI  + Y DGVIL AD+R T    ++++   K+  L  N++CC +G+AADT+    
Sbjct: 22  RMGTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVAD 81

Query: 427 SVASQLELQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVD-RTGPHIYCI 594
            +   L + R+  G    V TAATL   M ++ +  + A L++ G D +TG  +Y I
Sbjct: 82  LLKYYLSMYRIQFGHDPSVHTAATLASEMCYQNKNMLSAGLIVAGYDEKTGGDVYSI 138


>SPAC4A8.13c |pts1||20S proteasome component beta
           5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 272

 Score = 51.2 bits (117), Expect = 1e-07
 Identities = 36/147 (24%), Positives = 66/147 (44%), Gaps = 3/147 (2%)
 Frame = +1

Query: 163 IPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDK 342
           +PVP  S   + RN     K          GTT +   Y  G+++  D+RA+   +++ +
Sbjct: 33  VPVPQSSL--YLRNLTDETKNKHCLIKMNHGTTTLAFRYQHGIVVCVDSRASAGPLIASQ 90

Query: 343 NCQKIHYLASNMYCCG--AGTAADTEMTTQSVASQLELQRLHTGRTVPVETAATLLKRML 516
             +K+  +  N Y  G  AG AAD +     +  +  L +L     + V  A+ +L  + 
Sbjct: 91  TVKKV--IEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRNKELISVSAASKILSNIT 148

Query: 517 FRYQGH-IGAALVLGGVDRTGPHIYCI 594
           + Y+G+ +    +L G  + G  +Y I
Sbjct: 149 YSYKGYGLSMGTMLAGTGKGGTALYYI 175


>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 247

 Score = 46.0 bits (104), Expect = 4e-06
 Identities = 31/130 (23%), Positives = 59/130 (45%), Gaps = 11/130 (8%)
 Frame = +1

Query: 232 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 411
           A +A K G+T +G+   D V+LG + R T + ++   + +K+  + S++ C  +G  AD 
Sbjct: 27  AIEAIKLGSTAIGVKTKDAVVLGVEKRLT-SPLMESHSVEKLFEIDSHIGCAISGLTADA 85

Query: 412 EMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLFRY-QGH----------IGAALVLG 558
               +    Q +  R        +E+    +  +  R+ +G            G AL++ 
Sbjct: 86  RTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVALLIA 145

Query: 559 GVDRTGPHIY 588
           G+D  GP +Y
Sbjct: 146 GIDEHGPQLY 155


>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
           3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 25/91 (27%), Positives = 49/91 (53%), Gaps = 1/91 (1%)
 Frame = +1

Query: 265 VGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQL 444
           +GI+  DG++L A+ + T   +  +++ +K++++  NM C  AG  AD  +   + A ++
Sbjct: 35  LGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILI-NYARRV 93

Query: 445 ELQRLHT-GRTVPVETAATLLKRMLFRYQGH 534
             Q L T    +P E    L++R+    QG+
Sbjct: 94  GQQYLQTFNEEMPCE---QLVRRVCDLKQGY 121


>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 245

 Score = 35.1 bits (77), Expect = 0.008
 Identities = 31/143 (21%), Positives = 59/143 (41%), Gaps = 6/143 (4%)
 Frame = +1

Query: 178 FSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKI 357
           FS   F  N  L Q  + A  A   G T VGI   DGV+L  + + T    +   + +K+
Sbjct: 7   FSLTTFSPNGKLVQIEY-ALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIG-ASLEKV 64

Query: 358 HYLASNMYCCGAGTAADTEMTTQS--VASQLELQRLHT----GRTVPVETAATLLKRMLF 519
             +  ++    +G   D  +        +Q   ++++      + +  E A+ + +    
Sbjct: 65  CAITPDIGMVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASVMQESTQS 124

Query: 520 RYQGHIGAALVLGGVDRTGPHIY 588
                 G +L++ G+D  GP +Y
Sbjct: 125 GGVRPFGVSLLVAGMDEKGPSLY 147


>SPAC22F8.06 |pam1||20S proteasome component beta
           6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 225

 Score = 33.9 bits (74), Expect = 0.018
 Identities = 20/96 (20%), Positives = 45/96 (46%), Gaps = 1/96 (1%)
 Frame = +1

Query: 235 PKATKTGTTIVGIIYADG-VILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 411
           P     GTT+   I  DG  IL  DTR+     ++ +   ++H +  ++    +G  AD 
Sbjct: 8   PYVQNGGTTVA--IAGDGFAILAGDTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEADA 65

Query: 412 EMTTQSVASQLELQRLHTGRTVPVETAATLLKRMLF 519
               + +  +++L   +  R +  ++ A +++ +L+
Sbjct: 66  LALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLY 101


>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 262

 Score = 31.9 bits (69), Expect = 0.073
 Identities = 16/73 (21%), Positives = 39/73 (53%)
 Frame = +1

Query: 250 TGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQS 429
           TG++++ + +ADGV++ AD  A+  ++    + +++  +  N      G  +D +   Q 
Sbjct: 42  TGSSVLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQ-IQR 100

Query: 430 VASQLELQRLHTG 468
           +  +LE++  + G
Sbjct: 101 LLEKLEIKEGNYG 113


>SPCC1795.04c |||20S proteasome component alpha
           7|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 253

 Score = 28.7 bits (61), Expect = 0.68
 Identities = 29/126 (23%), Positives = 47/126 (37%), Gaps = 5/126 (3%)
 Frame = +1

Query: 232 APKATKTGTTIVGIIYADGVILGADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADT 411
           A KA +  +T +GI   DGVIL  +   T   +    N  +I  +  ++     G   D 
Sbjct: 26  AYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVN-NRIGSVDRHIGIATTGFIPDG 84

Query: 412 EMTTQSVASQLELQRLHTGRTVPVETAATLLKR--MLFRYQGHI---GAALVLGGVDRTG 576
           +   +    +    R + G  +P    A  L     LF     +   G    +   D  G
Sbjct: 85  QHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATYDSEG 144

Query: 577 PHIYCI 594
           PH+Y +
Sbjct: 145 PHLYMV 150


>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 995

 Score = 25.4 bits (53), Expect = 6.3
 Identities = 11/37 (29%), Positives = 20/37 (54%)
 Frame = -2

Query: 594 NAVNMRTGSVNTSQNQSSPYMALIAK*HSFQQCGSSF 484
           N + M+  S+ T + + +PY  L+A     + CGS +
Sbjct: 861 NIIPMQKPSLTTHETKQNPYDLLVALDSDRKACGSLY 897


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,346,925
Number of Sequences: 5004
Number of extensions: 45001
Number of successful extensions: 101
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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