Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= P5PG0159
(316 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g08710.1 68417.m01439 hypothetical protein contains Pfam prof... 30 0.28
At4g36520.1 68417.m05185 trichohyalin-related low similarity to ... 29 0.50
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 29 0.65
At3g54290.1 68416.m06000 expressed protein contains Pfam PF03794... 29 0.87
At1g32750.1 68414.m04038 HAC13 protein (HAC13) identical to HAC1... 28 1.5
At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)... 27 2.0
At4g13780.1 68417.m02137 methionine--tRNA ligase, putative / met... 27 2.6
At5g22640.1 68418.m02645 MORN (Membrane Occupation and Recogniti... 27 3.5
At5g22340.1 68418.m02606 expressed protein 27 3.5
At4g09950.1 68417.m01628 avirulence-responsive family protein / ... 27 3.5
At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p... 27 3.5
At1g16730.1 68414.m02007 expressed protein 26 4.6
At5g15430.1 68418.m01806 calmodulin-binding protein-related has ... 26 6.1
At3g60320.1 68416.m06742 expressed protein contains Pfam profile... 26 6.1
At1g74700.1 68414.m08652 RNase Z identical to RNase Z (GI:209756... 26 6.1
At1g11790.1 68414.m01353 prephenate dehydratase family protein s... 26 6.1
At1g09470.1 68414.m01059 expressed protein ; expression supporte... 26 6.1
At1g08940.1 68414.m00995 phosphoglycerate/bisphosphoglycerate mu... 26 6.1
At5g16210.1 68418.m01894 HEAT repeat-containing protein contains... 25 8.1
At4g31805.1 68417.m04519 WRKY family transcription factor identi... 25 8.1
At4g14455.1 68417.m02229 Bet1-like SNARE 1-2 / Bet1 / Sft1-like ... 25 8.1
At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA methyltran... 25 8.1
At1g50820.1 68414.m05715 hypothetical protein 25 8.1
>At4g08710.1 68417.m01439 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 715
Score = 30.3 bits (65), Expect = 0.28
Identities = 17/48 (35%), Positives = 25/48 (52%)
Frame = +1
Query: 121 EDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKN 264
EDV EK D +++ D Q + ++EK +KD E EKEK+
Sbjct: 642 EDVVQEKDGDQEEDVVQEKDGDQEEDGVSKEKEQEKEKDPKEKEKEKD 689
>At4g36520.1 68417.m05185 trichohyalin-related low similarity to
SP|Q07283 Trichohyalin {Homo sapiens}
Length = 1400
Score = 29.5 bits (63), Expect = 0.50
Identities = 19/80 (23%), Positives = 38/80 (47%), Gaps = 1/80 (1%)
Frame = +1
Query: 79 RDVDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKE 258
R+ + NE+ + + E E+ K+ + EK Q+K + +E+N K+ +E +
Sbjct: 744 REKEENERRIKEAREKAELEQRLKATLEQEEK--ERQIKERQEREENERRAKEVLEQAEN 801
Query: 259 KNKFLNGIENFD-PTKLKHT 315
+ K +E + +LK T
Sbjct: 802 ERKLKEALEQKENERRLKET 821
Score = 29.1 bits (62), Expect = 0.65
Identities = 16/66 (24%), Positives = 32/66 (48%)
Frame = +1
Query: 88 DTNEKIVLPSAEDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNK 267
+ NE+ E E+ K + +K + +LK T +E+N ++AIE E+++ +
Sbjct: 786 EENERRAKEVLEQAENERKLKEALE--QKENERRLKETREKEENKKKLREAIELEEKEKR 843
Query: 268 FLNGIE 285
+ E
Sbjct: 844 LIEAFE 849
>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam
domain PF04931: DNA polymerase V
Length = 1306
Score = 29.1 bits (62), Expect = 0.65
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +1
Query: 199 TETQEKNPLPDKDAIEAEKEKNKFLNGIENFD 294
TE E + LPD ++ +K K + +N + N+D
Sbjct: 12 TELVENDNLPDSSIVKKKKSKTEKMNTVANYD 43
>At3g54290.1 68416.m06000 expressed protein contains Pfam PF03794:
Domain of Unknown function
Length = 350
Score = 28.7 bits (61), Expect = 0.87
Identities = 18/45 (40%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Frame = +1
Query: 148 KSLFDGIEKFDSSQLK---HTETQEKNPLPDKDAIEAEKEKNKFL 273
++LF +F S Q+ H E +EK+ LP +A E KEK K L
Sbjct: 252 EALFSLASRFKSLQMMCKTHFEEEEKDLLPMVEAAEMGKEKQKKL 296
>At1g32750.1 68414.m04038 HAC13 protein (HAC13) identical to HAC13
[Arabidopsis thaliana] gi|21105767|gb|AAM34782; contains
Pfam domains, PF00439: Bromodomain and PF00240:
Ubiquitin family
Length = 1919
Score = 27.9 bits (59), Expect = 1.5
Identities = 17/59 (28%), Positives = 25/59 (42%)
Frame = +1
Query: 85 VDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEK 261
V + E +LP E +T SL FD E QE+ P + ++E EK +
Sbjct: 112 VVSEEDHLLPKKEYFSTAVALGSLKSRASVFDDEDYDEEEEQEEEQAPVEKSLETEKRE 170
>At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)
nearly identical to SF2/ASF-like splicing modulator
Srp30 [Arabidopsis thaliana] GI:4775270
Length = 268
Score = 27.5 bits (58), Expect = 2.0
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = +2
Query: 122 KTSPLRRPRSLYSTVSRS 175
++ PL R RSLYS+VSRS
Sbjct: 229 RSRPLSRSRSLYSSVSRS 246
>At4g13780.1 68417.m02137 methionine--tRNA ligase, putative /
methionyl-tRNA synthetase, putative / MetRS, putative
similar to methionyl-tRNA synthetase [Oryza sativa]
GI:4091008; contains Pfam profiles PF00133: tRNA
synthetases class I (I, L, M and V), PF01588: Putative
tRNA binding domain
Length = 797
Score = 27.1 bits (57), Expect = 2.6
Identities = 19/58 (32%), Positives = 28/58 (48%), Gaps = 2/58 (3%)
Frame = +1
Query: 106 VLPSAEDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPD--KDAIEAEKEKNKFL 273
V+P A D + KSL + +EKF + ++ E EK L K A+ E N +L
Sbjct: 427 VIPDAHDAESHSLTKSLAEKVEKFVA---EYVEAMEKVKLKQGLKTAMLISSEGNYYL 481
>At5g22640.1 68418.m02645 MORN (Membrane Occupation and Recognition
Nexus) repeat-containing protein contains Pfam profile
PF02493: MORN repeat
Length = 871
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/39 (30%), Positives = 23/39 (58%)
Frame = +1
Query: 136 EKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAE 252
EK+ K + DG+EK+ + K E +++ + + +EAE
Sbjct: 581 EKSVKPMLDGLEKWTEEKKKAYEERKEMIQQELELVEAE 619
>At5g22340.1 68418.m02606 expressed protein
Length = 323
Score = 26.6 bits (56), Expect = 3.5
Identities = 17/46 (36%), Positives = 23/46 (50%)
Frame = +1
Query: 76 LRDVDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDSSQLKHTETQE 213
L +D N SA V + T++SL DG + +S LK TQE
Sbjct: 87 LESIDVNSNGASSSA--VELDDTRESLIDGADDDESGFLKLKPTQE 130
>At4g09950.1 68417.m01628 avirulence-responsive family protein /
avirulence induced gene (AIG1) family protein similar to
AIG1 protein SP:P54120 [Arabidopsis thaliana]; contains
Pfam PF04548: AIG1 family;
Length = 336
Score = 26.6 bits (56), Expect = 3.5
Identities = 16/58 (27%), Positives = 28/58 (48%)
Frame = +1
Query: 136 EKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLK 309
EK+ + +GI++ S+QLK + K L A E EK +N I+ +++
Sbjct: 254 EKSHNEMLEGIKEKISNQLKESLEDVKEQLAKAQAEREETEKK--MNEIQKLSSDEIR 309
>At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p80
subunit, putative contains 5 WD-40 repeats (PF00400);
similar to katanin p80 subunit (GI:3005601)
[Strongylocentrotus purpuratus]
Length = 1180
Score = 26.6 bits (56), Expect = 3.5
Identities = 18/60 (30%), Positives = 27/60 (45%)
Frame = +1
Query: 121 EDVATEKTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPT 300
+ VA +KS DG +K D+ +E +NP D+I + NKF + PT
Sbjct: 436 QSVAVGYLKKSGLDGEKKLDTETAFDSEMSGRNPYDADDSI-IKSITNKFEQALLPESPT 494
>At1g16730.1 68414.m02007 expressed protein
Length = 202
Score = 26.2 bits (55), Expect = 4.6
Identities = 16/59 (27%), Positives = 25/59 (42%), Gaps = 4/59 (6%)
Frame = +1
Query: 37 DLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATEK----TQKSLFDGIEKFDSSQLKHT 201
D +G L + T +++PS TEK +K DG E+ D S+ K +
Sbjct: 121 DFAGAAQGMTKDALHRIKTQLAVIVPSVSPAVTEKIVDDAEKEAIDG-EEEDESKSKES 178
>At5g15430.1 68418.m01806 calmodulin-binding protein-related has
weak similarity to calmodulin-binding proteins
Length = 478
Score = 25.8 bits (54), Expect = 6.1
Identities = 15/36 (41%), Positives = 17/36 (47%)
Frame = -1
Query: 292 RSSRCRSGICSFPSPLR*HLCPEAGSSPESRCASAG 185
R SR SG + SPLR + SP RC S G
Sbjct: 70 RVSRSFSGAINLDSPLRKKALTKPLLSPSRRCDSVG 105
>At3g60320.1 68416.m06742 expressed protein contains Pfam profiles:
PF04782: protein of unknown function (DUF632), PF04783:
protein of unknown function (DUF630)
Length = 796
Score = 25.8 bits (54), Expect = 6.1
Identities = 18/64 (28%), Positives = 27/64 (42%)
Frame = -1
Query: 199 CASAGSNQTSRYRRIKTSGSSQWRRLQQTEAQSFHWCRRHGDSWC*SLRADSSGLCDLQP 20
CA++ + RR K + R +++ H H D +C SLR S L
Sbjct: 3 CAASKLDNEDAVRRCK----DRRRLMKEAVYARHHLAAAHAD-YCRSLRITGSALSSFAS 57
Query: 19 GDPL 8
G+PL
Sbjct: 58 GEPL 61
>At1g74700.1 68414.m08652 RNase Z identical to RNase Z (GI:20975609)
[Arabidopsis thaliana]
Length = 280
Score = 25.8 bits (54), Expect = 6.1
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +1
Query: 136 EKTQKSLFDGIEKFDSSQLKH 198
++T +SLF+ K DSS+LKH
Sbjct: 87 KETVESLFEVHRKLDSSELKH 107
>At1g11790.1 68414.m01353 prephenate dehydratase family protein
similar to gi|2392772 and is a member of the PF|00800
Prephenate dehydratase family. ESTs gb|T21562 and
gb|T21062 come from this gene
Length = 392
Score = 25.8 bits (54), Expect = 6.1
Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 1/60 (1%)
Frame = -1
Query: 271 GICSFPSPLR*HLCP-EAGSSPESRCASAGSNQTSRYRRIKTSGSSQWRRLQQTEAQSFH 95
G+ F + R C E SS R +A + R +K S S + Q+T++ SFH
Sbjct: 23 GLAEFDADKRRRFCLWECSSSASQRAVTAIEGEIPFSRELKKS-SDELGLTQETQSLSFH 81
>At1g09470.1 68414.m01059 expressed protein ; expression supported
by MPSS
Length = 336
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = +1
Query: 88 DTNEKIVLPSAEDVATEKTQKSLFD 162
+ NEK+ A DVATEK K L D
Sbjct: 76 ELNEKVEQIRASDVATEKFVKELAD 100
>At1g08940.1 68414.m00995 phosphoglycerate/bisphosphoglycerate
mutase family protein contains Pfam profile PF00300:
phosphoglycerate mutase family
Length = 281
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/36 (36%), Positives = 17/36 (47%)
Frame = +1
Query: 16 PRAAGRTDLKSQLEGFNTSCLRDVDTNEKIVLPSAE 123
P D+ ++ F S RDVD N V PS+E
Sbjct: 139 PEGESAADVYDRVSSFLESMWRDVDMNRHQVDPSSE 174
>At5g16210.1 68418.m01894 HEAT repeat-containing protein contains
Pfam profile PF02985: HEAT repeat
Length = 1180
Score = 25.4 bits (53), Expect = 8.1
Identities = 19/66 (28%), Positives = 29/66 (43%)
Frame = +1
Query: 7 LVDPRAAGRTDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATEKTQKSLFDGIEKFDSS 186
L++ A GR++ E + C ++ +LP ED + K L E+F SS
Sbjct: 727 LLEIYAEGRSEWP-MFEWMHVDCFANL-LQLACMLPQKEDHLRNRITKFLLAVSERFGSS 784
Query: 187 QLKHTE 204
L H E
Sbjct: 785 YLTHIE 790
>At4g31805.1 68417.m04519 WRKY family transcription factor identical
to WRKY DNA-binding protein 18 (WRKY18) GI:13506730 from
[Arabidopsis thaliana]
Length = 344
Score = 25.4 bits (53), Expect = 8.1
Identities = 17/71 (23%), Positives = 25/71 (35%)
Frame = -1
Query: 277 RSGICSFPSPLR*HLCPEAGSSPESRCASAGSNQTSRYRRIKTSGSSQWRRLQQTEAQSF 98
R G+C + + H E E N+ R + K + S W+ + AQ
Sbjct: 246 RYGVCPYELDKKLHELLETRQQEELVKLETALNRVERRLQEKETEVSWWKDAARLLAQRV 305
Query: 97 HWCRRHGDSWC 65
R G WC
Sbjct: 306 PESSRSGLEWC 316
>At4g14455.1 68417.m02229 Bet1-like SNARE 1-2 / Bet1 / Sft1-like
SNARE 14b / BS14b (BET12) identical to Bet1/Sft1-like
SNARE BS14b (GP|14029182) {Arabidopsis thaliana}
Length = 130
Score = 25.4 bits (53), Expect = 8.1
Identities = 10/47 (21%), Positives = 27/47 (57%)
Frame = +1
Query: 142 TQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGI 282
++ SLFDG++ + +L+ + + + + +A+E +++ FL +
Sbjct: 10 SRTSLFDGLDGLEEGRLRASSSYAHDERDNDEALENLQDRVSFLKRV 56
>At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA
methyltransferase family protein similar to
N2,N2-dimethylguanosine tRNA methyltransferase [Homo
sapiens] GI:11066198; contains Pfam profile PF02005:
N2,N2-dimethylguanosine tRNA methyltransferase
Length = 599
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +1
Query: 205 TQEKNPLPDKDAIEAEKEKNKFLNGIENFD 294
T+ + + +KDA EA KE+ NG+ N D
Sbjct: 62 TRPASKVIEKDASEASKEETPSENGMNNGD 91
>At1g50820.1 68414.m05715 hypothetical protein
Length = 528
Score = 25.4 bits (53), Expect = 8.1
Identities = 10/32 (31%), Positives = 17/32 (53%)
Frame = -1
Query: 187 GSNQTSRYRRIKTSGSSQWRRLQQTEAQSFHW 92
G + +R+ +K S S+ WR ++ SF W
Sbjct: 287 GEPRLARWCDMKQSTSNAWRIFNNSKMDSFEW 318
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.307 0.127 0.345
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,433,840
Number of Sequences: 28952
Number of extensions: 119288
Number of successful extensions: 460
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 450
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 460
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 330493944
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.6 bits)
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