BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0144 (501 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 224 5e-60 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 135 3e-33 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 132 4e-32 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 126 2e-30 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 83 2e-17 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 81 7e-17 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 68 7e-13 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 55 7e-09 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.056 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 0.30 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 27 1.2 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.1 SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces... 27 2.1 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 2.8 SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineur... 26 2.8 SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|... 25 4.8 SPBC14F5.05c |sam1||S-adenosylmethionine synthetase |Schizosacch... 25 4.8 SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosacc... 25 6.4 SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-... 25 8.4 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 8.4 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 224 bits (548), Expect = 5e-60 Identities = 104/115 (90%), Positives = 108/115 (93%), Gaps = 1/115 (0%) Frame = +1 Query: 43 EAEQKR-YLTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 219 EA+ KR LTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NR Sbjct: 57 EAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNR 116 Query: 220 EKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYAL 384 EKMTQI+FETFN PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYAL Sbjct: 117 EKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYAL 171 Score = 72.1 bits (169), Expect = 4e-14 Identities = 33/37 (89%), Positives = 36/37 (97%) Frame = +2 Query: 389 HAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIV 499 HAI+RLDLAGRDLTDYLMKIL ERGY+F+TTAEREIV Sbjct: 173 HAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIV 209 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 135 bits (327), Expect = 3e-33 Identities = 60/115 (52%), Positives = 86/115 (74%), Gaps = 1/115 (0%) Frame = +1 Query: 43 EAEQKR-YLTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 219 EAE R L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANR Sbjct: 57 EAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANR 116 Query: 220 EKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYAL 384 EKM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L Sbjct: 117 EKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVL 171 Score = 44.0 bits (99), Expect = 1e-05 Identities = 19/37 (51%), Positives = 26/37 (70%) Frame = +2 Query: 389 HAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIV 499 H + RLD+AGRD T YL+ +L +GY+F TA+ E V Sbjct: 173 HLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETV 209 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 132 bits (318), Expect = 4e-32 Identities = 60/118 (50%), Positives = 86/118 (72%), Gaps = 2/118 (1%) Frame = +1 Query: 43 EAEQKR-YLTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKAN 216 EA+ R L ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N Sbjct: 61 EAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRN 120 Query: 217 REKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALXS 390 +EK+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++ S Sbjct: 121 KEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPS 178 Score = 36.3 bits (80), Expect = 0.003 Identities = 16/36 (44%), Positives = 25/36 (69%) Frame = +2 Query: 392 AILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIV 499 AI + ++ GRD+TDYL L + G+ ++AE+EIV Sbjct: 179 AIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIV 214 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 126 bits (303), Expect = 2e-30 Identities = 58/116 (50%), Positives = 76/116 (65%), Gaps = 8/116 (6%) Frame = +1 Query: 67 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 246 +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148 Query: 247 TFNTPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTVPIYEGYALXS 390 +FN +Y+A+QAVL+L AS + TG V+DSGDGV+H +P+ EGY + S Sbjct: 149 SFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGS 204 Score = 24.6 bits (51), Expect = 8.4 Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 2/35 (5%) Frame = +2 Query: 392 AILRLDLAGRDLTDYLMKILTERGY--SFTTTAER 490 +I + LAGRD+T ++ +L +R S TAER Sbjct: 205 SIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER 239 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 83.4 bits (197), Expect = 2e-17 Identities = 40/107 (37%), Positives = 59/107 (55%) Frame = +1 Query: 64 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 243 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 244 ETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYAL 384 E+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 81.4 bits (192), Expect = 7e-17 Identities = 39/117 (33%), Positives = 66/117 (56%), Gaps = 2/117 (1%) Frame = +1 Query: 79 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 258 P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 259 PAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEGYALXSRHPASGLGRSR 423 P++ I + S Y + + ++GIVL+ G+ SH +P+ G + S G S+ Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAKRISWGGSQ 201 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 68.1 bits (159), Expect = 7e-13 Identities = 27/96 (28%), Positives = 55/96 (57%) Frame = +1 Query: 88 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 267 +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 268 YVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEG 375 Y+ QAV +A+ ++T +++D G + P+ +G Sbjct: 134 YLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDG 169 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 54.8 bits (126), Expect = 7e-09 Identities = 34/116 (29%), Positives = 53/116 (45%), Gaps = 4/116 (3%) Frame = +1 Query: 79 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 246 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 247 TFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALXSRHPASGLG 414 P +A + ++ LYA G G+V+D G + PI +G + + LG Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLG 227 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.9 bits (69), Expect = 0.056 Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +1 Query: 139 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 282 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.5 bits (63), Expect = 0.30 Identities = 26/81 (32%), Positives = 32/81 (39%) Frame = +2 Query: 137 PSTMSSVSPPRNIPSFSXXXXXXXXXXXXXXXXXCSKHSTRPPCTSPSKPCSRCTRPVVP 316 P++ SS S P IP S C+ ++ PP T S S P VP Sbjct: 140 PTSTSSTSIP--IPPTSTSSTDTNSNPLPTTSTSCTTSTSIPP-TGGSSSLSTPITPTVP 196 Query: 317 PVSCWTPATVSPTPYPSTRDT 379 P S T +T P P ST T Sbjct: 197 PTS--TSSTSIPIPPTSTSST 215 Score = 27.1 bits (57), Expect = 1.6 Identities = 18/47 (38%), Positives = 22/47 (46%) Frame = +2 Query: 239 CSKHSTRPPCTSPSKPCSRCTRPVVPPVSCWTPATVSPTPYPSTRDT 379 C+ ++ PP T S S P VPP S T +T P P ST T Sbjct: 115 CTTSTSIPP-TGGSSSLSTPITPTVPPTS--TSSTSIPIPPTSTSST 158 Score = 25.8 bits (54), Expect = 3.7 Identities = 25/81 (30%), Positives = 31/81 (38%) Frame = +2 Query: 137 PSTMSSVSPPRNIPSFSXXXXXXXXXXXXXXXXXCSKHSTRPPCTSPSKPCSRCTRPVVP 316 P++ SS S P IP S C+ ++ P T S S P VP Sbjct: 197 PTSTSSTSIP--IPPTSTSSTDTNSSPLPTTSTSCTTSTSIP--TGGSSSLSTPITPTVP 252 Query: 317 PVSCWTPATVSPTPYPSTRDT 379 P S T +T P P ST T Sbjct: 253 PTS--TSSTSIPIPPTSTSST 271 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 27.5 bits (58), Expect = 1.2 Identities = 10/26 (38%), Positives = 20/26 (76%) Frame = +2 Query: 392 AILRLDLAGRDLTDYLMKILTERGYS 469 A+ R+D+ G+ LT+YL ++++ R Y+ Sbjct: 182 AVKRVDVGGKLLTNYLKEVISYRKYN 207 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 26.6 bits (56), Expect = 2.1 Identities = 14/36 (38%), Positives = 17/36 (47%) Frame = +2 Query: 263 PCTSPSKPCSRCTRPVVPPVSCWTPATVSPTPYPST 370 P T+P P T PV P S P+ P P PS+ Sbjct: 1037 PSTAPPVPIPTSTPPV-PKSSSGAPSAPPPVPAPSS 1071 >SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 26.6 bits (56), Expect = 2.1 Identities = 16/59 (27%), Positives = 22/59 (37%), Gaps = 1/59 (1%) Frame = +2 Query: 260 PPCTSPSKPCSRCTRPVVPPVSCWTPATVSPTPYPS-TRDTHSXHAILRLDLAGRDLTD 433 PP P + T PV PV+ A P P P+ + H + D A + D Sbjct: 367 PPPVMPQPAAAAVTTPVAAPVAAAAAAVPVPPPAPAPAAAPAAEHVVALYDYAAQAAGD 425 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 2.8 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 181 LLTEAPLNPKANREKMTQIMFETFN 255 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineurin deletion Rnc1|Schizosaccharomyces pombe|chr 3|||Manual Length = 398 Score = 26.2 bits (55), Expect = 2.8 Identities = 13/29 (44%), Positives = 16/29 (55%) Frame = +2 Query: 284 PCSRCTRPVVPPVSCWTPATVSPTPYPST 370 P SR T+P+ S +P VSP PST Sbjct: 262 PVSRLTQPLPSLASTASPQQVSPPAAPST 290 >SPAC6G10.10c |||human hmmtag2 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 194 Score = 25.4 bits (53), Expect = 4.8 Identities = 13/23 (56%), Positives = 15/23 (65%) Frame = +3 Query: 300 RVRSYHRYRAGLRRRCLPHRTHL 368 R RS HR+ G RR P+RTHL Sbjct: 142 RTRSNHRH--GSHRRHEPYRTHL 162 >SPBC14F5.05c |sam1||S-adenosylmethionine synthetase |Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 25.4 bits (53), Expect = 4.8 Identities = 14/37 (37%), Positives = 19/37 (51%) Frame = +3 Query: 282 SRALAVRVRSYHRYRAGLRRRCLPHRTHLRGIRTPLT 392 S A A R + AGL RRCL ++ G+ PL+ Sbjct: 280 SAAYAARWIAKSLVAAGLARRCLVQLSYAIGVAEPLS 316 >SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 663 Score = 25.0 bits (52), Expect = 6.4 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = +2 Query: 281 KPCSRCTRPVVPPVSCWTPATVSPTPYP 364 KP S+ TRP +P + T +V P P P Sbjct: 65 KPSSKATRPYIPSYTRLT-YSVPPLPIP 91 >SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-forming]|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 24.6 bits (51), Expect = 8.4 Identities = 15/49 (30%), Positives = 20/49 (40%) Frame = +1 Query: 22 PGCRNRHEAEQKRYLTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPE 168 PG N E + Y Y IV N D + IW ++ L V P+ Sbjct: 120 PGEINFDEENWEAYRAANYAFAEAIVKNLQDGDLIWVQDYH--LMVLPQ 166 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 24.6 bits (51), Expect = 8.4 Identities = 13/27 (48%), Positives = 13/27 (48%) Frame = +2 Query: 299 TRPVVPPVSCWTPATVSPTPYPSTRDT 379 T P S TPA V P P PST T Sbjct: 833 TSPYATTSSHITPADVHPLPPPSTSTT 859 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,100,910 Number of Sequences: 5004 Number of extensions: 43118 Number of successful extensions: 171 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 166 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 198176188 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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