BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= P5PG0132 (504 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC21E11.03c |pcr1|mts2|transcription factor Pcr1|Schizosacchar... 26 2.8 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 4.9 SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb... 25 4.9 SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc... 25 6.5 >SPAC21E11.03c |pcr1|mts2|transcription factor Pcr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 171 Score = 26.2 bits (55), Expect = 2.8 Identities = 12/31 (38%), Positives = 15/31 (48%) Frame = +2 Query: 5 RSKLVDPPGCRNRHEAKGVGIPPTQPPPSDN 97 RS L D N ++ + P QPPP DN Sbjct: 83 RSVLTDFQTAHNALHSQHMAYRPVQPPPGDN 113 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 25.4 bits (53), Expect = 4.9 Identities = 12/35 (34%), Positives = 22/35 (62%) Frame = +1 Query: 340 AVNNLDHQDEQE*KVKCKIYS*LNVLNNLFIQVLT 444 AVNN DH ++Q+ + + S L V++ L ++ +T Sbjct: 730 AVNNSDHVEKQKRLAQARFISELCVIHMLDVRAIT 764 >SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 695 Score = 25.4 bits (53), Expect = 4.9 Identities = 8/19 (42%), Positives = 15/19 (78%) Frame = +1 Query: 421 NLFIQVLTALQNFIPETCG 477 N FI ++ ++Q+F+ E+CG Sbjct: 427 NKFINLVNSIQSFLKESCG 445 >SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces pombe|chr 2|||Manual Length = 987 Score = 25.0 bits (52), Expect = 6.5 Identities = 18/62 (29%), Positives = 27/62 (43%), Gaps = 2/62 (3%) Frame = +2 Query: 80 PPPSDNTANN--RKICSKIPKHQVCTMDRAQIINGALESKELRSDTYELVRLRRCLRDQA 253 PP D TA + K + + K VCT+ + E + R+ Y + +R LRD Sbjct: 646 PPGLDATAYSSWEKGDNFVTKKNVCTVMNLWVQKYFFEDLKARNTLYLISEMRTFLRDHV 705 Query: 254 *P 259 P Sbjct: 706 VP 707 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,043,624 Number of Sequences: 5004 Number of extensions: 39714 Number of successful extensions: 92 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 89 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 91 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 200198394 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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